Regeneração de plantas a partir do cultivo de óvulos de Citrus reticulata cv. Dancy

Undeveloped ovules of Citrus reticulata Blanco cv. Dancy were cultivated with the objective to determinate the effect of pollination, harvesting time and culture conditions appropiated for induction of embryogenesis and embryo production. It was verified the effect of in vivo grafting of cotiledonary embryos, in ten-month-old rootstock obtained from seeds, on the rapidity of obtention of disease-free plants and on the reduction of the juvenility stage. Ovule originated from pollinated and non-pollinated flowers collected 7, 14, 21 and 28 days after anthesis grown under light and dark conditions were cultured in basic medium MT, added with 500 mg.L-1 of malt extract. Cotiledonary embryos, 4-6 mm long, obtained in vitro were grafted in vivo on rootstocks. Embryos were directly obtained from ovule culture, however it was observed the formation of a high number of abnormal embryos in all treatments. Whole plants were developed on the same basic medium. It was not observed the formation of callus until 120 days. Differences related to the percentage of ovules that induced embryos and the number of embryos were observed in all treatments. The success rate of embryo grafting was 23%, however, it was observed a fast seedling development, which is a promissing technique for breeding programs and maybe in the mass propagation of virus-free plants, in case the juvenile phase be reduced.Este trabalho foi desenvolvido com o objetivo de verificar o efeito da polinização, época de coleta, condição de cultivo de óvulos não desenvolvidos de Citrus reticulata Blanco cv. Dancy, quanto à indução de embriogênese e o número de embriões desenvolvidos e verificar o efeito da enxertia in vivo de embriões cotiledonares, em porta-enxertos obtidos a partir de sementes, sobre a rapidez de obtenção de plantas sadias e redução do período de juvenilidade. Óvulos originados de flores com e sem polinização, coletados aos 7, 14, 21 e 28 dias, mantidos sob luz e no escuro foram cultivados em meio básico MT, suplementado com 500 mg.L-1 de extrato de malte. Embriões cotiledonares, obtidos diretamente do cultivo de óvulos, com 4-6 mm de comprimento foram enxertados diretamente em porta-enxertos com 10 meses de idade. Não houve formação de nenhum calo até os 120 dias após o início do cultivo. Entre os tratamentos observaram-se diferenças quanto à porcentagem de óvulos que induziram embriões e ao número de embriões formados. Plantas inteiras foram desenvolvidas no mesmo meio básico. A porcentagem de pegamento dos enxertos foi de 23%, entretanto, foi observado um rápido desenvolvimento das mudas, mostrando boas perspectivas desta técnica em programas de melhoramento e talvez na propagação de plantas livres de viroses, caso a fase juvenil seja reduzida

Como promover um envelhecer saudável durante a pandemia de COVID-19? Uma revisão integrativa

COVID-19 was a disease that stopped the world in mid-March 2020 and continues with alarming data so far, making it necessary to propose preventive measures, including social isolation. The article aims to analyze the possibilities of promoting healthy aging in the context of the COVID-19 pandemic. The research refers to an integrative review that includes the identification and analysis of scientific publications about the promotion of healthy aging in the face of the challenges of the pandemic of COVID-19. Eight (08) articles were selected from those surveyed, which culminated in the approach of three categories: SOCIAL ISOLATION X VULNERABILITY OF THE ELDERLY, MENTAL HEALTH X COVID-19 and NURSING ACTIONS FOR HEALTHY AGING, where it was possible to understand the importance of promoting healthy aging mainly in the context of the COVID-19 pandemic, where social isolation creates barriers in communication, in activities of daily living and in nursing care.O COVID-19 foi uma doença que parou o mundo em meados de março de 2020 e segue com dados alarmantes até o momento, tornando necessárias propostas de medidas preventivas, dentre elas o isolamento social. O artigo tem por objetivo analisar as possibilidades de promoção do envelhecer saudável no contexto da pandemia de COVID-19. A pesquisa refere-se a uma revisão integrativa que engloba a identificação e análise de publicações científicas acerca da promoção do envelhecer saudável diante dos desafios da pandemia de COVID-19. Foram selecionados oito (08) artigos dentre os pesquisados, que culminaram na abordagem de três categorias: relações ISOLAMENTO SOCIAL X VULNERABILIDADE DO IDOSO, SAÚDE MENTAL X COVID-19 e AÇÕES DE ENFERMAGEM PARA O ENVELHECER SAUDÁVEL, onde pôde-se entender a importância da promoção do envelhecer saudável principalmente no contexto da pandemia do COVID-19, onde o isolamento social cria barreiras na comunicação, nas atividades de vida diária e no cuidar em enfermagem

Atypical Copy Number Abnormalities In 22q11.2 Region: Report Of Three Cases.

The 22q11.2 Deletion Syndrome (22q11.2DS) is the most common microdeletion syndrome in humans, with a highly variable phenotype. This chromosomal region contains low copy repeat (LCR) sequences that mediate non-allelic homologous recombination which predispose to copy number abnormalities at this locus. This article describes three patients investigated for suspicion of 22q11.2DS presenting atypical copy number abnormalities overlapping or not with the common ∼3 Mb deletion. They were investigated by G-banding karyotype, Multiplex-ligation dependent probe amplification (MLPA) and array Genomic Hibridization (aGH). Clinical and molecular data were compared with literature, in order to contribute to genotype-phenotype correlation. Atypical chromosomal abnormalities were detected: 3.6 Mb deletion at 22q11.21-q11.23 between LCRs B-F in patient 1 and approximately 1.5 Mb deletion at 22q11.21-q11.22 between LCRs D-E in patients 2 and 3. The breakpoints detected in patient 1 have not been previously described. These findings exemplify the complexity and genetic heterogeneity observed in 22q11.2 region and corroborates the idea that genetic modifiers contribute to the phenotypic variability observed in proximal and distal 22q11.2 deletion syndromes.56515-2

NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics

Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data

Growing knowledge: an overview of Seed Plant diversity in Brazil

Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora