Effect of Processing Parameters on the Density and Microstructure of Direct Laser Sintered Al-12Si Powders

The effect of processing parameters on the sintering behaviour of gas atomised Al-12Si powders has been investigated. Laser power, scanning rate, scan spacing and layer thickness are found to control the densification and the resultant microstructural characteristics of the laser sintered parts. It was found that sintered density increased as the energy density increased reaching a maximum of 80.2% at an energy input per unit volume of 67 J mm-3. For parts produced with a slightly lower power density (50 J mm-3), the microstructure consisted of fine dendrites with interconnected porosity while parts fabricated with a slightly higher power density (100 J mm-3) were noted to have a preponderance of coarse dendrites with a discontinuous network of irregular shaped pores surrounded by a fully dense aluminium-silicon matrix.Mechanical Engineerin

Selective Laser Sintering of Polymer Nanocomposites

This paper describes the fabrication and characterization of polymer nanocomposite (PNC) materials for use in the selective laser sintering (SLS) process. PNC materials are of great interest generally because of their excellent physical properties, and offer excellent potential in rapid manufacturing of structural polymeric parts. Three different nano additive materials have been used: cerium oxide IV, yttrium stabilized zirconia, and layered Hectorite clay. These materials have been used to reinforce PA6 polymer using solution blending and spray drying to create powder with particle sizes in the range of 5-40 µm. The mechanical properties and microstructure of the PNC materials have been evaluated and the results compared to those of unfilled polymer.Mechanical Engineerin

The luminosity-dependent clustering of Hα emitters from z~0.8 to z~2.2 with HiZELS

Poster presented at the conference Galaxy evoltion Across Time, 12-16 June, Paris, France We study the clustering of star-forming galaxies, using halo models to derive dark matter halo masses. Typical galaxies in our samples are star-forming centrals, residing in host halos of mass 10^12 M_solar. We find strong trends between galaxy Hα luminosity and dark matter halo mass at all redshifts. See also https://arxiv.org/abs/1704.0547

No dependence of radio properties of brightest group galaxies on the luminosity gap

We study the radio and optical properties of the brightest group galaxies (BGGs) in a sample of galaxy groups from the SDSS DR7. The luminosity difference between the BGG and the second ranked galaxy in the group (known as the luminosity, or magnitude, gap) has been used as a probe for the level of galaxy interaction for the BGG within the group. We study the properties of BGGs with magnitude gaps in the range 0-2.7 magnitudes, in order to investigate any relation between luminosity gap and the radio properties of the BGG. In order to eliminate selection biases, we ensure that all variations in stellar mass are accounted for. We then confirm that, at fixed stellar mass, there are no significant variations in the optical properties of the BGGs over the full range of luminosity gaps studied. We compare these optical results with the EAGLE hydrodynamical simulations and find broad consistency with the observational data. Using EAGLE we also confirm that no trends begin to arise in the simulated data at luminosity gaps beyond our observational limits. Finally, we find that, at fixed stellar mass, the fraction of BGGs that are radio-loud also shows no trends as a function of luminosity gap. We examine how the BGG offset from the center of group may affect the radio results and find no significant trend for the fraction of radio-loud BGGs with magnitude gap in either the BGG samples with greater or less than 100kpc offset from the center of group.Comment: Accepted for publication in A

Use of modified U1 snRNAs to inhibit HIV-1 replication

Control of RNA processing plays a central role in regulating the replication of HIV-1, in particular the 3′ polyadenylation of viral RNA. Based on the demonstration that polyadenylation of mRNAs can be disrupted by the targeted binding of modified U1 snRNA, we examined whether binding of U1 snRNAs to conserved 10 nt regions within the terminal exon of HIV-1 was able to inhibit viral structural protein expression. In this report, we demonstrate that U1 snRNAs complementary to 5 of the 15 regions targeted result in significant suppression of HIV-1 protein expression and viral replication coincident with loss of viral RNA. Suppression of viral gene expression is dependent upon appropriate assembly of a U1 snRNP particle as mutations of U1 snRNA that affect binding of U1 70K or Sm proteins significantly reduced efficacy. However, constructs lacking U1A binding sites retained significant anti-viral activity. This finding suggests a role for these mutants in situations where the wild-type constructs cause toxic effects. The conserved nature of the sequences targeted and the high efficacy of the constructs suggests that this strategy has significant potential as an HIV therapeutic

Disappearing galaxies: the orientation dependence of JWST-bright, HST-dark, star-forming galaxy selection

Galaxies that are invisible in deep optical-NIR imaging but detected at longer wavelengths have been the focus of several recent observational studies, with speculation that they could constitute a substantial missing population and even dominate the cosmic star formation rate density at $z\gtrsim4$. The depths now achievable with JWST at the longest wavelengths probed by HST, coupled with the transformative resolution at longer wavelengths, are already enabling detailed, spatially-resolved characterisation of sources that were invisible to HST, often known as `HST-dark' galaxies. However, until now, there has been little theoretical work to compare against. We present the first simulation-based study of this population, using highly-resolved galaxies from the Feedback in Realistic Environments (FIRE) project, with multi-wavelength images along several lines of sight forward-modelled using radiative transfer. We naturally recover a population of modelled sources that meet commonly-used selection criteria ($H_{\rm{AB}}>27\,\rm{mag}$ and $H_{\rm{AB}}-\rm{F444W}>2.3$). These simulated HST-dark galaxies lie at high redshifts ($z=4-7$), have high levels of dust attenuation ($A_{V}=2-4$), and display compact recent star formation ($R_{1/2,\,\rm{4.4\,\mu\rm{m}}}\lesssim1\,\rm{kpc}$). Orientation is very important: for all but one of the 17 simulated galaxy snapshots with HST-dark sightlines, there exist other sightlines that do not meet the criteria. This result has important implications for comparisons between observations and models that do not resolve the detailed star-dust geometry, such as semi-analytic models or coarsely-resolved hydrodynamical simulations. Critically, we demonstrate that HST-dark sources are not an unexpected or exotic population, but a subset of high-redshift, highly-dust-attenuated sources viewed along certain lines of sight.Comment: 12 pages, 8 figures. Accepted for publication in Ap

Loss of miR-200c: A Marker of Aggressiveness and Chemoresistance in Female Reproductive Cancers

We focus on unique roles of miR-200c in breast, ovarian, and endometrial cancers. Members of the miR-200 family target ZEB1, a transcription factor which represses E-cadherin and other genes involved in polarity. We demonstrate that the double negative feedback loop between miR-200c and ZEB1 is functional in some, but not all cell lines. Restoration of miR-200c to aggressive cancer cells causes a decrease in migration and invasion. These effects are independent of E-cadherin status. Additionally, we observe that restoration of miR-200c to ovarian cancer cells causes a decrease in adhesion to laminin. We have previously reported that reintroduction of miR-200c to aggressive cells that lack miR-200c expression restores sensitivity to paclitaxel. We now prove that this ability is a result of direct targeting of class III beta-tubulin (TUBB3). Introduction of a TUBB3 expression construct lacking the miR-200c target site into cells transfected with miR-200c mimic results in no change in sensitivity to paclitaxel. Lastly, we observe a decrease in proliferation in cells transfected with miR-200c mimic, and cells where ZEB1 is knocked down stably, demonstrating that the ability of miR-200c to enhance sensitivity to paclitaxel is not due to an increased proliferation rate

Field measurements of trace gases and aerosols emitted by peat fires in Central Kalimantan, Indonesia, during the 2015 El Nino

Abstract. Peat fires in Southeast Asia have become a major annual source of trace gases and particles to the regional–global atmosphere. The assessment of their influence on atmospheric chemistry, climate, air quality, and health has been uncertain partly due to a lack of field measurements of the smoke characteristics. During the strong 2015 El Niño event we deployed a mobile smoke sampling team in the Indonesian province of Central Kalimantan on the island of Borneo and made the first, or rare, field measurements of trace gases, aerosol optical properties, and aerosol mass emissions for authentic peat fires burning at various depths in different peat types. This paper reports the trace gas and aerosol measurements obtained by Fourier transform infrared spectroscopy, whole air sampling, photoacoustic extinctiometers (405 and 870 nm), and a small subset of the data from analyses of particulate filters. The trace gas measurements provide emission factors (EFs; grams of a compound per kilogram biomass burned) for up to  ∼  90 gases, including CO2, CO, CH4, non-methane hydrocarbons up to C10, 15 oxygenated organic compounds, NH3, HCN, NOx, OCS, HCl, etc. The modified combustion efficiency (MCE) of the smoke sources ranged from 0.693 to 0.835 with an average of 0.772 ± 0.053 (n  =  35), indicating essentially pure smoldering combustion, and the emissions were not initially strongly lofted. The major trace gas emissions by mass (EF as g kg−1) were carbon dioxide (1564 ± 77), carbon monoxide (291 ± 49), methane (9.51 ± 4.74), hydrogen cyanide (5.75 ± 1.60), acetic acid (3.89 ± 1.65), ammonia (2.86 ± 1.00), methanol (2.14 ± 1.22), ethane (1.52 ± 0.66), dihydrogen (1.22 ± 1.01), propylene (1.07 ± 0.53), propane (0.989 ± 0.644), ethylene (0.961 ± 0.528), benzene (0.954 ± 0.394), formaldehyde (0.867 ± 0.479), hydroxyacetone (0.860 ± 0.433), furan (0.772 ± 0.035), acetaldehyde (0.697 ± 0.460), and acetone (0.691 ± 0.356). These field data support significant revision of the EFs for CO2 (−8 %), CH4 (−55 %), NH3 (−86 %), CO (+39 %), and other gases compared with widely used recommendations for tropical peat fires based on a lab study of a single sample published in 2003. BTEX compounds (benzene, toluene, ethylbenzene, xylenes) are important air toxics and aerosol precursors and were emitted in total at 1.5 ± 0.6 g kg−1. Formaldehyde is probably the air toxic gas most likely to cause local exposures that exceed recommended levels. The field results from Kalimantan were in reasonable agreement with recent lab measurements of smoldering Kalimantan peat for “overlap species,” lending importance to the lab finding that burning peat produces large emissions of acetamide, acrolein, methylglyoxal, etc., which were not measurable in the field with the deployed equipment and implying value in continued similar efforts. The aerosol optical data measured include EFs for the scattering and absorption coefficients (EF Bscat and EF Babs, m2 kg−1 fuel burned) and the single scattering albedo (SSA) at 870 and 405 nm, as well as the absorption Ångström exponents (AAE). By coupling the absorption and co-located trace gas and filter data we estimated black carbon (BC) EFs (g kg−1) and the mass absorption coefficient (MAC, m2 g−1) for the bulk organic carbon (OC) due to brown carbon (BrC). Consistent with the minimal flaming, the emissions of BC were negligible (0.0055 ± 0.0016 g kg−1). Aerosol absorption at 405 nm was  ∼  52 times larger than at 870 nm and BrC contributed  ∼  96 % of the absorption at 405 nm. Average AAE was 4.97 ± 0.65 (range, 4.29–6.23). The average SSA at 405 nm (0.974 ± 0.016) was marginally lower than the average SSA at 870 nm (0.998 ± 0.001). These data facilitate modeling climate-relevant aerosol optical properties across much of the UV/visible spectrum and the high AAE and lower SSA at 405 nm demonstrate the dominance of absorption by the organic aerosol. Comparing the Babs at 405 nm to the simultaneously measured OC mass on filters suggests a low MAC ( ∼  0.1) for the bulk OC, as expected for the low BC/OC ratio in the aerosol. The importance of pyrolysis (at lower MCE), as opposed to glowing (at higher MCE), in producing BrC is seen in the increase of AAE with lower MCE (r2 =  0.65)

Solid-state and solution-phase conformations of pseudoproline-containing dipeptides

The conformations of 14 threonine-derived pseudoproline-containing dipeptides (including four d-allo-Thr derivatives) have been investigated by NMR. In solution, the major conformer observed for all dipeptides is that in which the amide bond between the pseudoproline and the preceding amino acid is cis. For dipeptides in which the N-terminus is protected, the ratio of cis- to trans-conformers does not depend significantly on the side chain of the N-terminal amino acid, or the stereochemistry of the Thr residue. However, for dipeptides bearing a free N-terminus, there are significant differences in the ratios of cis- to trans-conformers depending on the side chain present. Three dipeptides were crystallized and their X-ray structures determined. In two cases, (benzyloxycarbonyl (Cbz)-Val-Thr(ΨMe,Mepro)-OMe and Cbz-Val-Thr(ΨMe,Mepro)-OH), the dipeptides adopt a trans-conformation in the solid state, in contrast to the structures observed in solution. In the third case, (9-fluorenylmethoxycarbonyl (Fmoc)-Val-d-allo-Thr(ΨMe,Mepro)-OH), a cis-amide geometry is observed. These structural differences are attributed to crystal-packing interactions