5,978 research outputs found
A Project Based Approach to Statistics and Data Science
In an increasingly data-driven world, facility with statistics is more
important than ever for our students. At institutions without a statistician,
it often falls to the mathematics faculty to teach statistics courses. This
paper presents a model that a mathematician asked to teach statistics can
follow. This model entails connecting with faculty from numerous departments on
campus to develop a list of topics, building a repository of real-world
datasets from these faculty, and creating projects where students interface
with these datasets to write lab reports aimed at consumers of statistics in
other disciplines. The end result is students who are well prepared for
interdisciplinary research, who are accustomed to coping with the
idiosyncrasies of real data, and who have sharpened their technical writing and
speaking skills
Gait analysis in a <i>Mecp2</i> knockout mouse model of Rett syndrome reveals early-onset and progressive motor deficits
Rett syndrome (RTT) is a genetic disorder characterized by a range of features including cognitive impairment, gait abnormalities and a reduction in purposeful hand skills. Mice harbouring knockout mutations in the <i>Mecp2</i> gene display many RTT-like characteristics and are central to efforts to find novel therapies for the disorder. As hand stereotypies and gait abnormalities constitute major diagnostic criteria in RTT, it is clear that motor and gait-related phenotypes will be of importance in assessing preclinical therapeutic outcomes. We therefore aimed to assess gait properties over the prodromal phase in a functional knockout mouse model of RTT. In male <i>Mecp2</i> knockout mice, we observed alterations in stride, coordination and balance parameters at 4 weeks of age, before the onset of other overt phenotypic changes as revealed by observational scoring. These data suggest that gait measures may be used as a robust and early marker of <i>Mecp2</i>-dysfunction in future preclinical therapeutic studies
Role of Dicer Enzyme in the Regulation of Store Operated Calcium Entry (SOCE) in CD4+ T Cells
Background/Aims: Activation of T cell receptors (TCRs) in CD4+ T cells leads to a cascade of signalling reactions including increase of intracellular calcium (Ca2+) levels with subsequent Ca2+ dependent stimulation of gene expression, proliferation, cell motility and cytokine release. The increase of cytosolic Ca2+ results from intracellular Ca2+ release with subsequent activation of store-operated Ca2+ entry (SOCE). Previous studies suggested miRNAs are required for the development and functions of CD4+ T cells. An enzyme called Dicer is required during the process of manufacturing mature miRNAs from the precursor miRNAs. In this study, we explored whether loss of Dicer in CD4+ T cells affects SOCE and thus Ca2+ dependent regulation of cellular functions. Methods: We tested the expression of Orai1 by q-RT-PCR and flow cytometry. Further, we measured SOCE by an inverted phase-contrast microscope with the Incident-light fluorescence illumination system using Fura-2. Intracellular Ca2+ was also measured by flow cytometry using Ca2+ sensitive dye Fluo-4. Results: We found that in Dicer deficient (DicerΔ/Δ) mice Orai1 was downregulated at mRNA and protein level in CD4+ T cells. Further, SOCE was significantly smaller in DicerΔ/Δ CD4+ T cells than in CD4+ T cells isolated from wild-type (Dicerfl/fl) mice. Conclusion: Our data suggest that miRNAs are required for adequate Ca2+ entry into CD4+ T cells and thus triggering of Ca2+ sensitive immune functions
Ab Initio Liquid Hydrogen Muon Cooling Simulations with ELMS in ICOOL
This paper presents new theoretical results on the passage of muons through
liquid hydrogen which have been confirmed in a recent experiment. These are
used to demonstrate that muon bunches may be compressed by ionisation cooling
more effectively than suggested by previous calculations.
Muon cooling depends on the differential cross section for energy loss and
scattering of muons. We have calculated this cross section for liquid H2 from
first principles and atomic data, avoiding traditional assumptions. Thence, 2-D
probability maps of energy loss and scattering in mm-scale thicknesses are
derived by folding, and stored in a database. Large first-order correlations
between energy loss and scattering are found for H2, which are absent in other
simulations. This code is named ELMS, Energy Loss & Multiple Scattering. Single
particle trajectories may then be tracked by Monte Carlo sampling from this
database on a scale of 1 mm or less. This processor has been inserted into the
cooling code ICOOL. Significant improvements in 6-D muon cooling are predicted
compared with previous predictions based on GEANT. This is examined in various
geometries. The large correlation effect is found to have only a small effect
on cooling. The experimental scattering observed for liquid H2 in the MUSCAT
experiment has recently been reported to be in good agreement with the ELMS
prediction, but in poor agreement with GEANT simulation.Comment: 6 pages, 3 figure
Initial development of a high-pressure crystal growth facility: Center director's discretionary fund
A low-cost, flexible, high-pressure (600 psi) system for crystal growth and related thermophysical properties measurements was designed, assembled, and tested. The furnace system includes a magnetically coupled translation mechanism that eliminates the need for a high-pressure mechanical feedthru. The system is currently being used for continuing crystal growth experiments and thermophysical properties measurements on several material systems including Hg(1-x)Cd(x)Te, Hg(1-x)Zn(x)Te, and Hg(1-x)Zn(x)Se
From chemical Langevin equations to Fokker-Planck equation: application of Hodge decomposition and Klein-Kramers equation
The stochastic systems without detailed balance are common in various
chemical reaction systems, such as metabolic network systems. In studies of
these systems, the concept of potential landscape is useful. However, what are
the sufficient and necessary conditions of the existence of the potential
function is still an open problem. Use Hodge decomposition theorem in
differential form theory, we focus on the general chemical Langevin equations,
which reflect complex chemical reaction systems. We analysis the conditions for
the existence of potential landscape of the systems. By mapping the stochastic
differential equations to a Hamiltonian mechanical system, we obtain the
Fokker-Planck equation of the chemical reaction systems. The obtained
Fokker-Planck equation can be used in further studies of other steady
properties of complex chemical reaction systems, such as their steady state
entropies.Comment: 6 pages, 0 figure, submitted to J. Phys. A: Math. Theo
A Cost-Effective Design for a Neutrino Factory
There have been active efforts in the U.S., Europe, and Japan on the design
of a Neutrino Factory. This type of facility produces intense beams of
neutrinos from the decay of muons in a high energy storage ring. In the U.S., a
second detailed Feasibility Study (FS2) for a Neutrino Factory was completed in
2001. Since that report was published, new ideas in bunching, cooling and
acceleration of muon beams have been developed. We have incorporated these
ideas into a new facility design, which we designate as Study 2B (ST2B), that
should lead to significant cost savings over the FS2 design.Comment: 46 pages, 38 figures; to be submitted to Physical Review Special
Topics: Accelerators and Beam
Pamela: development of the RF system for a non-relativistic non-scaling FFAG
The PAMELA project(Particle Accelerator For MEdical
Applications) currently consists of the design of a particle
therapy facility. The project, which is in the design phase,
contains Non-Scaling FFAG, particle accelerator capable
of rapid beam acceleration, giving a pulse repetition rate of
1kHz, far beyond that of a conventional synchrotron. To
realise the repetition rate, a key component of the accelerator
is the rf accelerating system. The combination of a high
energy gain per turn and a high repetition rate is a significant
challenge. In this paper, options for the rf system of
the proton ring and the status of development are presented
Structured evaluation of virtual environments for special-needs education
This paper describes the development of a structured approach to evaluate experiential and communication virtual learning environments (VLEs) designed specifically for use in the education of children with severe learning difficulties at the Shepherd special needs school in Nottingham, UK. Constructivist learning theory was used as a basis for the production of an evaluation framework, used to evaluate the design of three VLEs and how they were used by students with respect to this learning theory. From an observational field study of student-teacher pairs using the VLEs, 18 behaviour categories were identified as relevant to five of the seven constructivist principles defined by Jonassen (1994). Analysis of student-teacher behaviour was used to provide support for, or against, the constructivist principles. The results show that the three VLEs meet the constructivist principles in very different ways and recommendations for design modifications are put forward
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