Conceptual Ecological Modelling of Shallow Sublittoral Sand Habitats to Inform Indicator Selection

The purpose of this study is to produce a series of conceptual ecological models (CEMs) which represent shallow sublittoral sand habitats in the UK. CEMs are diagrammatic representations of the influences and processes which occur within an ecosystem. They can be used to identify critical aspects of an ecosystem which may be taken forward for further study, or serve as the basis for the selection of indicators for environmental monitoring purposes. The models produced by this project are control diagrams, representing the unimpacted state of the environment free from anthropogenic pressures. The project scope included the Marine Strategy Framework Directive (MSFD) predominant habitat type ‘shallow sublittoral sand’. This definition includes those habitats which fall into the EUNIS Level 4 classifications A5.23 Infralittoral Fine Sand, A5.24 Infralittoral Muddy Sand, A5.25 Circalittoral Fine Sand and A5.26 Circalittoral Muddy Sand, along with their constituent Level 5 biotopes which are relevant to UK waters. A species list of characterising fauna to be included within the scope of the models was identified using an iterative process to refine the full list of species found within the relevant Level 5 biotopes. A literature review was conducted to gather evidence regarding species traits and information to inform the models. All information gathered during the literature review was entered into a data logging pro forma spreadsheet which accompanies this report. Wherever possible, attempts were made to collect information from UK-specific peer-reviewed studies, although other sources were used where necessary. All data gathered was subject to a detailed confidence assessment. Expert judgement by the project team was utilised to provide information for aspects of the models for which references could not be sourced within the project timeframe. A model hierarchy was developed based on groups of fauna with similar species traits which aligned with previous sensitivity studies of ecological groups. A general model was produced to indicate the high level drivers, inputs, biological assemblages, ecosystem processes and outputs which occur in shallow sublittoral sand habitats. In addition to this, four detailed sub-models were produced. Each focussed on a particular functional group of fauna within the habitat: “suspension and deposit feeding infauna”, “small mobile fauna and tube dwelling species”, “mobile epifauna, scavengers and predators”, and “attached epifauna and macroalgae”. Each sub-model is accompanied by an associated confidence model which presents confidence in the links between each model component. The models are split into seven levels and take spatial and temporal scale into account through their design, as well as magnitude and direction of influence. The seven levels include regional to global drivers, water column processes, local inputs/processes at the seabed, habitat and biological assemblage, output processes, local ecosystem functions, and regional to global ecosystem functions. The models indicate that whilst the high level drivers which affect each functional group are largely similar, the output processes performed by the biota and the resulting ecosystem functions vary both in number and importance between groups. Confidence within the models as a whole is generally high, reflecting the level of information gathered during the literature review. Important drivers which influence the ecosystem include factors such as wave exposure, depth, water currents, climate and propagule supply. These factors, in combination with seabed and water column processes such as primary production, seabed mobility, suspended sediments, water chemistry and temperature and recruitment define and influence the biological assemblages. In addition, the habitat sediment type plays an important factor in shaping the biology of the habitat. Conceptual Ecological Modelling of Shallow Sublittoral Sand Habitats Output processes are variable between functional faunal groups depending on the fauna present. Important processes include secondary production, biodeposition, bioturbation, bioengineering and the supply of propagules. These influence ecosystem functions at the local scale such as nutrient and biogeochemical cycling, supply of food resources, sediment stability, habitat provision and in some cases microbial activity. The export of biodiversity and organic matter, biodiversity enhancement and biotope stability are the resulting ecosystem functions which occur at the regional to global scale. Features within the models which are most useful for monitoring habitat status and change due to natural variation have been identified using the information gathered during the literature review, through interpretation of the models and through the application of expert judgement. Features within the models which may be useful for monitoring to identify anthropogenic causes of change within the ecosystem have also been identified. Physical and biological features of the ecosystem have mostly been identified as potential indicators to monitor natural variation, whilst physical features and output processes have predominantly been identified as most likely to indicate change due to anthropogenic pressures

Conceptual Ecological Modelling of Shallow Sublittoral Mixed Sediment Habitats to Inform Indicator Selection.

The purpose of this study is to produce a series of conceptual ecological models (CEMs) which represent shallow sublittoral mixed sediment habitats in the UK. CEMs are diagrammatic representations of the influences and processes which occur within an ecosystem. They can be used to identify critical aspects of an ecosystem which may be taken forward for further study, or serve as the basis for the selection of indicators for environmental monitoring purposes. The models produced by this project are control diagrams, representing the unimpacted state of the environment free from anthropogenic pressures. The project scope included the Marine Strategy Framework Directive (MSFD) predominant habitat type ‘shallow sublittoral mixed sediment’. This definition includes those habitats which fall into the EUNIS Level 4 classifications A5.43 Infralittoral Mixed Sediments and A5.44 Circalittoral Mixed Sediments, along with their constituent Level 5 biotopes which are relevant to UK waters. A species list of characterising fauna to be included within the scope of the models was identified using an iterative process to refine the full list of species found within the relevant Level 5 biotopes. A literature review was conducted to gather evidence regarding species traits and information to inform the models. All information gathered during the literature review was entered into a data logging pro forma spreadsheet which accompanies this report. Wherever possible, attempts were made to collect information from UK-specific peer-reviewed studies, although other sources were used where necessary. All data gathered was subject to a detailed confidence assessment. Expert judgement by the project team was utilised to provide information for aspects of the models for which references could not be sourced within the project timeframe. A model hierarchy was developed based on groups of fauna with similar species traits which aligned with previous sensitivity studies of ecological groups. A general model was produced to indicate the high level drivers, inputs, biological assemblages, ecosystem processes and outputs which occur in shallow sublittoral mixed sediment habitats. In addition to this, five detailed sub-models were produced. Each focussed on a particular functional group of fauna within the habitat: ‘temporary or permanently attached epifauna’, ‘mobile epifauna, scavengers and predators’, ‘suspension and deposit feeding fauna’, ‘temporary or permanently attached surface dwelling or shallowly buried larger bivalves’ and ‘small mobile epifauna and tube dwelling crustaceans’. Each sub-model is accompanied by an associated confidence model which presents confidence in the links between each model component. The models are split into seven levels and take spatial and temporal scale into account through their design, as well as magnitude and direction of influence. The seven levels include regional to global drivers, water column processes, local inputs/processes at the seabed, habitat and biological assemblage, output processes, local ecosystem functions, and regional to global ecosystem functions. The models indicate that whilst the high level drivers which affect each functional group are largely similar, the output processes performed by the biota and the resulting ecosystem functions vary both in number and importance between groups. Confidence within the models as a whole is generally high, reflecting the level of information gathered during the literature review. Important drivers which influence the ecosystem include factors such as wave exposure, depth, water currents, climate and propagule supply. These factors, in combination with seabed and water column processes such as primary production, seabed mobility, suspended sediments, water chemistry and temperature and recruitment define and Conceptual Ecological Modelling of Shallow Sublittoral Mixed Sediment Habitats influence the biological assemblages. In addition, the habitat sediment type plays an important factor in shaping the biology of the habitat. Output processes are variable between functional faunal groups depending on the fauna present. Important processes include secondary production, biodeposition, bioturbation, bioengineering and the supply of propagules. These influence ecosystem functions at the local scale such as nutrient and biogeochemical cycling, supply of food resources, sediment stability, habitat provision and in some cases microbial activity. The export of biodiversity and organic matter, biodiversity enhancement and biotope stability are the resulting ecosystem functions which occur at the regional to global scale. Features within the models which are most useful for monitoring habitat status and change due to natural variation have been identified using the information gathered during the literature review, through interpretation of the models and through the application of expert judgement. Features within the models which may be useful for monitoring to identify anthropogenic causes of change within the ecosystem have also been identified. Physical and biological features of the ecosystem have mostly been identified as potential indicators to monitor natural variation, whilst physical features and output processes have predominantly been identified as most likely to indicate change due to anthropogenic pressures

Conceptual Ecological Modelling of Shallow Sublittoral Mud Habitats to Inform Indicator Selection

The purpose of this study is to produce a series of Conceptual Ecological Models (CEMs) that represent the shallow sublittoral mud habitat in the UK. CEMs are diagrammatic representations of the influences and processes that occur within an ecosystem. The models can be used to identify critical aspects of an ecosystem that may be developed for further study, or serve as the basis for the selection of indicators for environmental monitoring purposes. The models produced by this project are ‘control diagrams’, representing the unimpacted state of the environment, free from anthropogenic pressures. It is intended that the models produced by this project will be used to guide indicator selection for the monitoring of this habitat in UK waters. CEMs will eventually be produced for a range of habitat types defined under the UK Marine Biodiversity Monitoring R&D Programme (UKMBMP), which, along with stressor models designed to show the interactions within impacted habitats, would form the basis of a robust method for indicator selection. This project builds on the work to develop CEMs for shallow sublittoral coarse sediment habitats (Alexander. 2014). The project scope included the Marine Strategy Framework Directive (MSFD) predominant habitat type ‘shallow sublittoral mud’. This definition includes those habitats that fall into the EUNIS Level 4 classifications A5.33 Infralittoral Sandy Mud, A5.34 Infralittoral Fine Mud, A5.35 Circalittoral Sandy Mud and A5.36 Circalittoral Fine Mud, along with their constituent Level 5 biotopes which are relevant to UK waters. A species list of characterising fauna to be included within the scope of the models was identified using an iterative process to refine the full list of species found within the relevant Level 5 biotopes. A literature review was conducted using a pragmatic and iterative approach to gather evidence regarding species traits and information that would be used to inform the models and the interactions that occur within the shallow sublittoral mud habitat. All information gathered during the literature review was entered into a data logging pro forma spreadsheet which accompanies this report. Wherever possible, attempts were made to collect information from UK-specific peer-reviewed studies, although other sources were used where necessary. All data gathered was subject to a detailed confidence assessment. Expert judgement by the project team was utilised to provide information for aspects of the models for which references could not be sourced within the project timeframe. A model hierarchy was developed based on groups of fauna with similar species traits which aligned with previous sensitivity studies of ecological groups. One general control model was produced that indicated the high level drivers, inputs, biological assemblages, ecosystem processes and outputs that occur in shallow sublittoral mud habitats. In addition to this, five detailed sub-models were produced, which each focussed on a particular functional group of fauna within the habitat: tube building fauna, burrowing fauna, suspension and deposit feeding infauna, mobile epifauna, scavengers and predators, and echinoderms and sessile epifauna. Each sub-model is accompanied by an associated confidence model that presents confidence in the links between each model component. The models are split into seven levels and take spatial and temporal scale into account through their design, as well as magnitude and direction of influence. The seven levels include regional to global drivers, water column processes, local inputs/processes at the seabed, habitat and biological assemblage, output processes, local ecosystem functions, and regional to global ecosystem functions. The models indicate that whereas the high level drivers which affect each functional group are largely similar, the output processes performed by the biota and the resulting ecosystem functions vary both in number and importance between groups. Confidence within the Conceptual Ecological Modelling of Shallow Sublittoral Mud Habitats models as a whole is generally high, reflecting the level of information gathered during the literature review. Important drivers that influence the ecosystem include factors such as wave exposure, depth, water currents, climate and propagule supply. These factors, in combination with seabed and water column processes, such as primary production, suspended sediments, water chemistry, temperature and recruitment define and influence the food sources consumed by the biological assemblages of the habitat, and the biological assemblages themselves. In addition, the habitat sediment type plays an important factor in shaping the biology of the habitat. Output processes performed by the biological assemblage are variable between functional faunal groups depending on the specific fauna present and the role they perform within the ecosystem. Important processes include secondary production, biodeposition, bioturbation, bioengineering and the supply of propagules; these in turn influence ecosystem functions at the local scale such as nutrient and biogeochemical cycling, supply of food resources, sediment stability, habitat provision and in some cases microbial activity. The export of biodiversity and organic matter, biodiversity enhancement and biotope stability are the resulting ecosystem functions that occur at the regional to global scale. Features within the models that are most useful for monitoring habitat status and change due to natural variation have been identified; as have those which may be useful for monitoring to identify anthropogenic causes of change within the ecosystem. Physical and chemical features of the ecosystem have mostly been identified as potential indicators to monitor natural variation, whilst biological factors have predominantly been identified as most likely to indicate change due to anthropogenic pressures

Photoelectrons in the Enceladus plume

The plume of Enceladus is a remarkable plasma environment containing several charged particle species. These include cold magnetospheric electrons, negative and positive water clusters, charged nanograins, and “magnetospheric photoelectrons” produced from ionization of neutrals throughout the magnetosphere near Enceladus. Here we discuss observations of a population newly identified by the Cassini Plasma Spectrometer (CAPS) electron spectrometer instrument—photoelectrons produced in the plume ionosphere itself. These were found during the E19 encounter, in the energetic particle shadow where penetrating particles are absent. Throughout E19, CAPS was oriented away from the ram direction where the clusters and nanograins are observed during other encounters. Plume photoelectrons are also clearly observed during the E9 encounter and are also seen at all other Enceladus encounters where electron spectra are available. This new population, warmer than the ambient plasma population, is distinct from, but adds to, the magnetospheric photoelectrons. Here we discuss the observations and examine the implications, including the ionization source these electrons provide

X-ray structure of a putative reaction intermediate of 5-aminolaevulinic acid dehydratase

The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 Å) resolution. The cyclic intermediate is bound covalently to Lys(263) with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C–C bond linking both substrates in the intermediate is formed before the C–N bond

The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.

The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha

Rotationally driven magnetic reconnection in Saturn's dayside

Magnetic reconnection is a key process that explosively accelerates charged particles, generating phenomena such as nebular flares, solar flares and stunning aurorae. In planetary magnetospheres, magnetic reconnection has often been identified on the dayside magnetopause and in the nightside magnetodisc, where thin-current-sheet conditions are conducive to reconnection. The dayside magnetodisc is usually considered thicker than the nightside due to the compression of solar wind, and is therefore not an ideal environment for reconnection. In contrast, a recent statistical study of magnetic flux circulation strongly suggests that magnetic reconnection must occur throughout Saturn’s dayside magnetosphere. Additionally, the source of energetic plasma can be present in the noon sector of giant planetary magnetospheres. However, so far, dayside magnetic reconnection has only been identified at the magnetopause. Here, we report direct evidence of near-noon reconnection within Saturn’s magnetodisc using measurements from the Cassini spacecraft. The measured energetic electrons and ions (ranging from tens to hundreds of keV) and the estimated energy flux of ~2.6 mW m–2 within the reconnection region are sufficient to power aurorae. We suggest that dayside magnetodisc reconnection can explain bursty phenomena in the dayside magnetospheres of giant planets, which can potentially advance our understanding of quasi-periodic injections of relativistic electrons6 and auroral pulsations

Reconnection acceleration in Saturn's dayside magnetodisc:a multicase study with Cassini

Recently, rotationally driven magnetic reconnection was firstly discovered in Saturn’s dayside magnetosphere (Guo et al. 2018). This newly confirmed process could potentially drive bursty phenomena at Saturn, i.e., pulsating energetic particles and auroral emissions. Using Cassini’s measurements of magnetic fields and charged particles, we investigate particle acceleration features during three magnetic reconnection events observed in Saturn’s dayside magnetodisc. The results suggest that the rotationally driven reconnection process plays a key role in producing energetic electrons (up to 100 keV) and ions (several hundreds of keV). In particular, we find that energetic oxygen ions are locally accelerated at all three reconnection sites. Isolated, multiple reconnection sites were recorded in succession during an interval lasting for much less than one Saturn rotation period. Moreover, a secondary magnetic island is reported for the first time at the dayside, collectively suggesting that the reconnection process is not steady and could be ‘drizzle-like’. This study demonstrates the fundamental importance of internally driven magnetic reconnection in accelerating particles in Saturn’s dayside magnetosphere, and likewise in the rapidly rotating Jovian magnetosphere and beyond