Virucidal activity of 70% Ethanol vs Enveloped and Non-Enveloped Viruses

Viruses contain genetic material packaged in a protein coat called the capsid. For some viruses, the capsid is surrounded by an envelope composed of a lipid bilayer derived from the host cell membrane (Figure 2). Virus structure determines the stability characteristics of the virus particle, such as resistance to chemical or physical inactivation (Lucas, 2010). To maintain a safe environment at the Institute for Antiviral Research, it is important to understand which viruses are resistant to which chemicals. Thus, the objective of this experiment was to test survival of the viruses listed in table 1, when exposed to various chemicals. Neutral red dye is routinely used in antiviral assays and 70% ethanol is common disinfectant used. MEM & H2O were used as negative controls. Mt. Dew was also tested because we thought it would be fun

Isosorbide dinitrate-hydralazine combination therapy in African Americans with heart failure

Despite significant improvement in therapy and management, heart failure remains a worrisome disease state that is especially problematic in special populations. African Americans suffer a disproportionately higher prevalence of heart failure when compared to other populations. It has been recently demonstrated that vasodilator therapy using the combination of isosorbide dinitrate (ISDN) and hydralazine (HYD) as an adjunct to background evidence-based therapy appears to display the strongest signal of benefit in reducing mortality and morbidity in the African American population. Through review of the retrospective and more recent prospective data, we will focus on the benefit of ISDN-HYD as adjunctive therapy for use in African Americans with systolic heart failure on concomitant appropriate evidence based therapy. This review also closely examines some of the potential contributions to endothelial dysfunction in African Americans, and the relationship of vascular homeostasis and nitric oxide. The role of oxidative stress in left ventricular dysfunction will also be explored as a reduction of oxidative stress offers particular promise in the management of heart failure. Although neurohormonal blockade has been responsible for notable event reductions in patients with systolic heart failure, the addition of ISDN-HYD, vasodilator therapy that enhances nitric oxide and reduces oxidative stress, further improves quality of life and survival in African American patients with heart failure. These findings strongly imply that nitric oxide enhancement and/or oxidative stress reduction may be important new therapeutic directions in the management of heart failure

Measuring Market Saturation in the U.S. Casino Industry: An Analytical and Empirical Analysis

The national and regional economies in the U.S. remain on a slow growth trajectory, while the casino gaming industry has seen a rapid and ongoing expansion. Consequently, states, Native American tribes, and gaming operators have increasingly shifted their attention from gaming expansion to the problems of regional competition, cannibalization, market maturation, and market saturation. The question of “market saturation” has become a salient point of public policy debate and a topic that is now frequently raised in the industry and media. This paper analyzes the concept of saturation in the context of casino gaming markets and compares several metrics for measuring saturation. We examine several markets widely acknowledged and accepted by the industry as being “saturated” to assess the sufficiency of these metrics for determining whether a market is saturated

Psychosocial Variables as Prospective Predictors of Violent Events Among Adolescents

Violent events are main causes of mortality among children and include intentional (e.g., homicide) and unintentional (e.g., accidents) circumstances. This study investigated the prediction of the self-reported occurrence of 14 violent events among eighth-grade youth from psychosocial variables measured in these same youth in seventh grade. Psychosocial variables ineluded tobacco and alcohol use, demographic variables, interpersonal variables such as family conflict, and intrapersonal variables such as risk taking. An iterative procedure, involving selection of a set of predictors and a test of the correlation of the set of predictors to the set of events, provided support for an extension of problem behavior theory to violent events

Identification of Which High Risk Youth Smoke Cigarettes Regularly

This study investigated which variables distinguish high school-aged adolescents who identify themselves as members of a high-risk group and, among them, those who report regular (weekly) vs. light levels of cigarette smoking. Youth who identified with a high-risk group were most likely to report problem-prone characteristics, such as a preference to take risks and smoke cigarettes. Yet, only half of them reported regular levels of smoking. Two variables delineated light smoking among these youth: not having a close friend who smoked and placing an importance on health as a value. Development of new tobacco-use prevention strategies to impart health values is suggested

One-Year Prospective Prediction of Violence Perpetration Among High Risk Youth from Personal and Social-Environmental Variables

Objective: Measures of drug use, law-abidance beliefs, sensation seeking, fear of victimization, high-risk group identification, self- protection needs and behaviors, and demographics were investigated as longitudinal predictors of violence perpetration among 870 high-risk adolescents. Method: Self-reports from the same youth were obtained 1-year apart. Results: In addition to baseline violence perpetration, marijuana use, relatively youn1 age, male sex, high-risk group self-identification, low perceived efficacy of the police department, and nonavoidance of dangerous places predicted later perpetrated violence. Conclusion: Personal and social factors beyond baseline violent behavior predict risk for future violent behavior

Cannabinoid CB1 receptor inhibition blunts adolescent-typical increased binge alcohol and sucrose consumption in male C57BL/6J mice

Increased binge alcohol consumption has been reported among adolescents as compared to adults in both humans and rodent models, and has been associated with serious long-term health consequences. However, the neurochemical mechanism for age differences in binge drinking between adolescents and adults has not been established. The present study was designed to evaluate the mechanistic role of the cannabinoid CB1 receptor in adolescent and adult binge drinking. Binge consumption was established in adolescent and adult male C57BL/6J mice by providing access to 20% alcohol or 1% sucrose for 4h every other day. Pretreatment with the CB1 antagonist/inverse agonist AM-251 (0, 1, 3, and 10mg/kg) in a Latin square design dose-dependently reduced adolescent alcohol consumption to adult levels without altering adult intake. AM-251 (3mg/kg) also reduced adolescent but not adult sucrose consumption. Adolescent reductions in alcohol and sucrose were not associated with alterations in open-field locomotor activity or thigmotaxis. These findings point to age differences in CB1 receptor activity as a functional mediator of adolescent-typical increased binge drinking as compared to adults. Developmental alterations in endocannabinoid signaling in the adolescent brain may therefore be responsible for the drinking phenotype seen in this age group

The Photosynthetic Function of Manganese and Chloride

Author Institution: Charles F. Kettering Foundation, Yellow Springs, Ohi

Anxiety and depression in young and middle aged hypertensive and normotensive subjects

This study examined the effects of essential hypertension on measures of anxiety and depression for two age groups of hypertensive (free from hypertension-related pathology and/or cardiovascular disease) and normotensive subjects. Hypertensive subjects had significantly higher State Anxiety scores and Zung Depression scores than did normotensive subjects. These differences between the blood pressure groups were due largely to the scores of the younger hypertensive subjects. The results of the present study are consistent with previous results from our laboratory that have found that younger hypertensives differed (relative to controls) from middle aged hypertensives on measures, such as, symptoms reported on the Cornell Medical Index and WAIS Performance scores. The results of the present study were discussed within the context of age associated differences in response to hypertension and factors that might account for these differences

Children's biobehavioral reactivity to challenge predicts DNA methylation in adolescence and emerging adulthood.

A growing body of research has documented associations between adverse childhood environments and DNA methylation, highlighting epigenetic processes as potential mechanisms through which early external contexts influence health across the life course. The present study tested a complementary hypothesis: indicators of children's early internal, biological, and behavioral responses to stressful challenges may also be linked to stable patterns of DNA methylation later in life. Children's autonomic nervous system reactivity, temperament, and mental health symptoms were prospectively assessed from infancy through early childhood, and principal components analysis (PCA) was applied to derive composites of biological and behavioral reactivity. Buccal epithelial cells were collected from participants at 15 and 18 years of age. Findings revealed an association between early life biobehavioral inhibition/disinhibition and DNA methylation across many genes. Notably, reactive, inhibited children were found to have decreased DNA methylation of the DLX5 and IGF2 genes at both time points, as compared to non-reactive, disinhibited children. Results of the present study are provisional but suggest that the gene's profile of DNA methylation may constitute a biomarker of normative or potentially pathological differences in reactivity. Overall, findings provide a foundation for future research to explore relations among epigenetic processes and differences in both individual-level biobehavioral risk and qualities of the early, external childhood environment