ESCOLHAS LITERÁRIAS E USO DE ANTOLOGIAS POÉTICAS NA SALA DE AULA DO ENSINO MÉDIO

A seleção dos textos literários para trabalhar com as turmas do Ensino Médio tem sido um desafio para os(as) professores(as), uma vez que a autoria docente e os aspectos pedagógicos (curriculares, didáticos, etc.) atravessam tais escolhas. No intuito de refletir sobre a mediação do texto literário na escola, trazemos uma experiência recente com a construção de uma antologia poética a qual foi utilizada em um turma do 2º ano do Ensino Médio, no contexto do estágio supervisionado de um curso de Letras. A partir de tal experiência, discutiremos sobre os critérios e (im)possibilidades para organizar uma antologia para jovens do ensino médio, afastando-se das escolhas prescritas pelos exames vestibulares e pelos livros didáticos. Ao mesmo tempo, o uso da antologia implicou uma reflexão pedagógica sobre os modos de ler e de avaliar a compreensão do texto na escola, destacando os processos singulares e subjetivos de apreciação estética e ética dos leitores

CUIDADO DE ENFERMAGEM NA PERSPECTIVA DA PROMOÇÃO À SAÚDE DO ADOLESCENTE: REVISÃO DE ESCOPO

Objective: To map the scientific evidence on nursing care in adolescent health promotion. Method: Scoping review developed according to the JBI manual with a search conducted in MEDLINE, LILACS, SCOPUS, Web of Science, Cochrane, CINAHL and BDEnf databases, and in the Theses and Dissertations Bank of the CAPES Portal. The data were analyzed using descriptive statistics and narrative synthesis. Results: 16 studies published between 2000 and 2021 were included in this review. As for nursing care for adolescent health promotion, there are widely used actions and strategies, such as lectures, plays, educational sessions, interviews, nursing consultation, groups in message exchange applications, and others specific to schools or health services. Conclusion: Nursing care for adolescent health promotion requires the articulation between health services and school, and may use various strategies, requiring planning and use of specific instruments to improve the nursing approach and adolescent adherence.Objetivo: mapear la evidencia científica sobre los cuidados de enfermería en la promoción de la salud de los adolescentes. Método: Revisión del alcance desarrollada de acuerdo con el manual de JBI con búsqueda realizada en las bases de datos medline, LILACS, SCOPUS, Web of Science, Cochrane, CINAHL y BDEnf, y en la Base de Datos de Tesis y Disertaciones del Portal Capes, incluyendo estudios que abordaron la atención de enfermería centrándose en aspectos de la promoción de la salud del adolescente. Los datos fueron analizados a través de estadística descriptiva y síntesis narrativa. Resultados: En esta revisión se incluyeron 16 estudios, publicados entre 2000 y 2021. En cuanto a la atención de enfermería para la promoción de la salud de los adolescentes, existen acciones y estrategias de amplio uso, como conferencias, obras de teatro, sesiones educativas, entrevistas, consultas de enfermería, grupos en aplicaciones para intercambios de mensajes y otras específicas de escuelas o servicios de salud. Conclusión: La atención de enfermería para la promoción de la salud de los adolescentes requiere la articulación entre los servicios de salud y la escuela, y puede utilizar estrategias variadas, requiriendo planificación y uso de instrumentos específicos para mejorar el enfoque de enfermería y el adcoverage del adolescente.Objetivo: mapear as evidências científicas sobre o cuidado da enfermagem na promoção da saúde ao adolescente. Método: Revisão de escopo desenvolvida conforme o manual do JBI com busca realizada nas bases de dados MEDLINE, LILACS, SCOPUS, Web of Science, Cochrane, CINAHL e BDEnf, e no Banco de Teses e Dissertações do Portal CAPES, sendo incluídos os estudos que abordaram o cuidado de enfermagem enfocando aspectos da promoção à saúde do adolescente. Os dados foram analisados por meio da estatística descritiva e da síntese narrativa. Resultados: 16 estudos, publicados entre 2000 e 2021, foram incluídos nesta revisão. Quanto aos cuidados de enfermagem para a promoção da saúde de adolescentes, existem ações e estratégias de ampla utilização, como palestras, peças teatrais, sessões educativas, entrevistas, consulta de enfermagem, grupos em aplicativos de trocas de mensagens e outras específicas para escolas ou serviços de saúde. Conclusão: O cuidado de enfermagem para a promoção da saúde ao adolescente requer a articulação entre serviços de saúde e escola, podendo utilizar estratégias variadas, requerendo planejamento e utilização de instrumentos específicos para melhorar a abordagem de enfermagem e a adesão do adolescente.Objetivo: mapear as evidências científicas sobre o cuidado da enfermagem na promoção da saúde ao adolescente. Método: Revisão de escopo desenvolvida conforme o manual do JBI com busca realizada nas bases de dados MEDLINE, LILACS, SCOPUS, Web of Science, Cochrane, CINAHL e BDEnf, e no Banco de Teses e Dissertações do Portal CAPES, sendo incluídos os estudos que abordaram o cuidado de enfermagem enfocando aspectos da promoção à saúde do adolescente. Os dados foram analisados por meio da estatística descritiva e da síntese narrativa. Resultados: 16 estudos, publicados entre 2000 e 2021, foram incluídos nesta revisão. Quanto aos cuidados de enfermagem para a promoção da saúde de adolescentes, existem ações e estratégias de ampla utilização, como palestras, peças teatrais, sessões educativas, entrevistas, consulta de enfermagem, grupos em aplicativos de trocas de mensagens e outras específicas para escolas ou serviços de saúde. Conclusão: O cuidado de enfermagem para a promoção da saúde ao adolescente requer a articulação entre serviços de saúde e escola, podendo utilizar estratégias variadas, requerendo planejamento e utilização de instrumentos específicos para melhorar a abordagem de enfermagem e a adesão do adolescente

Application of multivariate curve resolution of experimental data generated by several analytical techniques : an exploratory overview of violacein biosynthesis

Orientador: Márcia Miguel Castro FerreiraTese (doutorado) - Universidade Estadual de Campinas, Instituto de QuimicaResumo: A aplicação de métodos de resolução de curvas multivariada (MCR-ALS e PARAFAC) a dados de espectroscopia de fluorescência multicomprimento de onda, absorção molecular e cromatografia líquida de ultra eficiência com detecção por espectrometria de massas foi avaliada como um nova e complementar metodologia para se obter informação inicial sobre a biossíntese de produtos naturais. Para demonstrar o poder dessa proposta, a biossíntese da violaceína pela Chromobacterium violaceum foi usada como exemplo. A violaceína é um metabólito secundário que exibe amplo espectro de atividade biológica e o entendimento de sua rota biossintética pode ser de grande interesse para o planejamento de fármacos. Para tanto, uma cultura da C. violaceum foi cultivada em biorreator, onde, a cada duas horas, alíquotas do meio de cultura eram coletadas para subsequentes análises. Na primeira parte deste trabalho, as matrizes de emissão-excitação das amostras revelaram o comportamento dinâmico de fluoróforos envolvidos na biossíntese. O método de análise de fatores paralelos (PARAFAC) foi aplicado para resolver os perfis de fluorescência, onde seis espectros puros de emissão e excitação foram obtidos e suas relações com a biossíntese da violaceína estabelecidas. Na segunda parte, o método de resolução de curvas multivariada por quadrados mínimos alternados (MCR-ALS) foi utilizado para extrair informações simultâneas dos dados gerados por UV-VIS, fluorescência e UPLC-MS das amostras coletadas durante a biossíntese. Cinco compostos foram resolvidos e informações espectrais e temporais dessas espécies foram obtidas. Compostos com massas moleculares de 453, 465 e 479 u, até então não reportados na literatura como intermediários dessa biossíntese, foram identificados. Por fim, a metodologia baseada na aplicação de métodos de resolução de curvas MCR-ALS e PARAFAC provou ser uma poderosa ferramenta para investigar a biossíntese de produtos naturaisAbstract:The application of Multivariate Curve Resolution methods (MCR-ALS and PARAFAC) into multi-wavelength fluorescence spectroscopy, molecular absorption and ultra-performance liquid chromatography coupled to mass spectrometry data was evaluated as a new and complementary approach for the initial analysis of the biosynthesis of fluorescent natural products. To demonstrate the usefulness of this methodology, violacein biosynthesis by Chromobacterium violaceum was used as an example. Violacein is a secondary metabolite that exhibits a broad spectrum of biological activities and the understanding of its biosynthetic pathway can be of great interest for drug design. Therefore, a culture of C. violaceum was grown in a bioreactor from which aliquots were collected every two hours for subsequent analysis. In the first part of this work, the excitation¿emission matrices of the samples have revealed the dynamic behavior of the fluorophores involved in this biosynthesis. The parallel factor analysis (PARAFAC) method was applied to resolve the fluorescence profiles where six pure emission and excitation spectra were obtained and their relationship with violacein biosynthesis established. In the second part, the multivariate curve resolution procedure based on alternating least squares optimization (MCR-ALS) was used for extracting information from fused data (UV-VIS absorption, fluorescence and ultra-performance liquid chromatography with mass spectrometry) gathered during the violacein biosynthesis. Five chemical components were resolved from which spectral and temporal information were obtained. Compounds with molecular masses 453, 465 and 479 u, until now unreported as intermediate of this biosynthesis, were identified. Finally, the methodology based on the application of curve resolution methods PARAFAC and MCR-ALS has proven to be a unique and powerful tool to achieve a deeper insight of the biosynthesis of natural productsDoutoradoFísico-QuímicaDoutor em Ciência

Exploring In Vivo Violacein Biosynthesis By Application Of Multivariate Curve Resolution On Fused Uv-vis Absorption, Fluorescence, And Liquid Chromatography-mass Spectrometry Data.

In this work, the application of multivariate curve resolution-alternating least squares (MCR-ALS) is proposed for extracting information from multitechnique fused multivariate data (UV-VIS absorption, fluorescence, and liquid chromatography-mass spectrometry) gathered during the biosynthesis of violacein pigment. Experimental data sets were pretreated and arranged in a row-wise augmented data matrix before their chemometric investigation. Five different chemical components were resolved. Kinetic and spectral information about these components were obtained and their relationship with violacein biosynthesis was established. Three new chemical compounds with molar masses of 453, 465, and 479 u, until now not reported in the literature, were identified and proposed as intermediates in the biosynthesis of other indolocarbazoles. The precursor (tryptophan), one intermediate (deoxyviolacein), and the final product (violacein) of violacein biosynthesis were identified and characterized using the proposed approach. The chemometric procedure based on the MCR-ALS method has proved to be a powerful tool to investigate violacein biosynthesis and its application can be easily extended to the study of other bioprocesses.4051293-30

Theoretical Study Of Acid-catalyzed Hydrolysis Of Epoxides.

A theoretical study of propylene oxide acid-catalyzed hydrolysis was performed by investigation of the S(N)1 and S(N)2-like mechanisms. By using chemometric tools, hierarchical cluster analysis (HCA), and principal component analysis (PCA), the MP2/6-311++G** level of theory was selected from HF, MP2, and DFT as the best method to describe the geometry of the basic skeleton (oxirane). At this level of theory, geometry optimizations, vibrational frequencies, intrinsic reaction coordinate (IRC), and other thermodynamic calculations have shown that the borderline S(N)2 mechanism is more favorable than pure S(N)2 and S(N)1 mechanisms in the gas phase. In the S(N)1 mechanism, the existence of the typical carbocation was not observed, and furthermore, the possibility of epoxide conversion to a protonated aldehyde was indicated, even in the presence of a water molecule (nucleophile). The Chelpg charge distribution of the reactants, steric hindrance, synchronous bond breaking-formation and trajectory angle of nucleophilic attack are discussed for pure and borderline S(N)2 mechanisms. Solvation effect calculations indicate that the pure S(N)2 mechanism is more favorable than borderline S(N)2 and S(N)1 mechanisms. This is discussed in terms of hydrogen bond formation.1145187-9

PARAFAC: adjustment for modeling consumer study covering probiotic and conventional yogurt

The feasibility of the parallel factors model (PARAFAC) as a modeling tool for consumer sensory acceptance data of complex food matrices was investigated. The attributes of aroma, flavor, taste, texture, and overall liking of probiotic and conventional yogurts were evaluated by 120 consumers using a 9-point hedonic hybrid scale. Six yogurt samples were used: three were prototypes supplemented with a glucose oxidase/glucose system, which is a potential oxygen scavenging system, and the other three were commercial yogurts. The yogurts supplemented with the glucose oxidase/glucose system presented similar sensory acceptance towards commercial probiotic and conventional yogurts (p > 0.05), besides having garnered lower mean scores for the sensory attributes. Appearance and overall liking were the most significant attributes for the first two components of the PARAFAC model. Finally, the advantage of using this method to explore the intrinsic nature of consumer sensory data is discussed and compared to principal component analysis (PCA)45121121

Parafac: adjustment for modeling consumer study covering probiotic and conventional yogurt

The feasibility of the parallel factors model (PARAFAC) as a modeling tool for consumer sensory acceptance data of complex food matrices was investigated. The attributes of aroma, flavor, taste, texture, and overall liking of probiotic and conventional yogurts were evaluated by 120 consumers using a 9-point hedonic hybrid scale. Six yogurt samples were used: three were prototypes supplemented with a glucose oxidase/glucose system, which is a potential oxygen scavenging system, and the other three were commercial yogurts. The yogurts supplemented with the glucose oxidase/glucose system presented similar sensory acceptance towards commercial probiotic and conventional yogurts (p > 0.05), besides having garnered lower mean scores for the sensory attributes. Appearance and overall liking were the most significant attributes for the first two components of the PARAFAC model. Finally, the advantage of using this method to explore the intrinsic nature of consumer sensory data is discussed and compared to principal component analysis (PCA). © 2011 Elsevier Ltd.The feasibility of the parallel factors model (PARAFAC) as a modeling tool for consumer sensory acceptance data of complex food matrices was investigated. The attributes of aroma, flavor, taste, texture, and overall liking of probiotic and conventional yogurts were evaluated by 120 consumers using a 9-point hedonic hybrid scale. Six yogurt samples were used: three were prototypes supplemented with a glucose oxidase/glucose system, which is a potential oxygen scavenging system, and the other three were commercial yogurts. The yogurts supplemented with the glucose oxidase/glucose system presented similar sensory acceptance towards commercial probiotic and conventional yogurts (p>0.05), besides having garnered lower mean scores for the sensory attributes. Appearance and overall liking were the most significant attributes for the first two components of the PARAFAC model. 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