The Effect of NAG–thiazoline on Morphology and Surface Hydrophobicity of Escherichia Coli

The β-hexosaminidase inhibitor and structural analog of the putative oxazolium reaction intermediate of lytic transglycosylases, N-acetylglucosamine thiazoline (NAG–thiazoline), was synthesized in 46% overall yield and tested as an inhibitor of Escherichia coli growth. NAG–thiazoline, at concentrations up to 1 mg/ml, was not found to affect the viability of E. coli DH5α

Autonomous Cars, Electric and Hybrid Cars, and Ridesharing: Perceptions vs. Reality

Autonomous Cars, Electric and Hybrid Cars, and Ridesharing are all important new technologies in today\u27s society that can have potentially large impacts on the environment in the future. This study was conducted to determine the differences in perceptions of Gettysburg College students regarding Autonomous Cars, Electric and Hybrid Cars, and Ridesharing and the reality of these topics in the real world. This paper also compares the perceptions of Environmental Studies majors/minors to the perceptions of other majors at Gettysburg College. The primary research was conducted by analyzing questions that were a part of a survey consisting of 16 questions which was administered to Gettysburg College students via Facebook class group pages and the Environmental Studies majors email alias. The study group consisted of 110 students with 31 of them being Environmental Studies majors/minors and 79 of them being non-Environmental Studies majors/minors. It was determined that there were no statistically significant differences between the Environmental Studies majors/minors and students that are other majors/minors at Gettysburg College. From our survey, we found that there is a distinct gap in knowledge on the current and future impacts on the environment from Autonomous Cars, Electric and Hybrid Cars, and Ridesharing. The questions that ask which power method produces more greenhouse gas emissions as well as the questions about the miles per gallon of participants’ personal vehicles were the most accurately answered. Overall, Gettysburg College students regardless of major or minor were found to have mostly inaccurate perceptions on the topics of Autonomous Cars, Electric and Hybrid Cars, and Ridesharing

Rhodium/phospholane-phosphite catalysts give unusually high regioselectivity in the enantioselective hydroformylation of vinyl arenes

Using the phospholane-phosphite ligand, BOBPHOS, almost perfect regioselectivities and high enantioselectivities (up to 92% ee) are observed in Rh catalysed enantioselective hydroformylation of vinyl arenes. This can be achieved under solvent-free conditions.PostprintPeer reviewe

Transitions in the morphological features, habitat use, and diet of young-of-the-year goosefish (Lophius americanus)

This study was designed to improve our understanding of transitions in the early life history and the distribution, habitat use, and diets for young-of-the-year (YOY) goosefish (Lophius americanus) and, as a result, their role in northeastern U.S. continental shelf ecosystems. Pelagic juveniles (>12 to ca. 50 mm total length [TL]) were distributed over most portions of the continental shelf in the Middle Atlantic Bight, Georges Bank, and into the Gulf of Maine. Most individuals settled by 50−85 mm TL and reached approximately 60−120 mm TL by one year of age. Pelagic YOY fed on chaetognaths, hyperiid amphipods, calanoid copepods, and ostracods, and benthic YOY had a varied diet of fishes and benthic crustaceans. Goosefish are widely scattered on the continental shelf in the Middle Atlantic Bight during their early life history and once settled, are habitat generalists, and thus play a role in many continental shelf habi

Evidence of traffic-related pollutant control in soil-based Sustainable Urban Drainage Systems (SUDS)

SUDS are being increasingly employed to control highway runoff and have the potential to protect groundwater and surface water quality by minimising the risks of both point and diffuse sources of pollution. While these systems are effective at retaining polluted solids by filtration and sedimentation processes, less is known of the detail of pollutant behaviour within SUDS structures. This paper reports on investigations carried out as part of a co-ordinated programme of controlled studies and field measurements at soft-engineered SUDS undertaken in the UK, observing the accumulation and behaviour of traffic-related heavy metals, oil and PAHs. The field data presented were collected from two extended detention basins serving the M74 motorway in the south-west of Scotland. Additional data were supplied from an experimental lysimeter soil core leaching study. Results show that basin design influences pollutant accumulation and behaviour in the basins. Management and/or control strategies are discussed for reducing the impact of traffic-related pollutants on the aqueous environment

Development of Preventive Measures to Reduce Mortalities for Holding Live Wild-caught Flounder in Recirculating Aquaculture Systems

A substantial high-value market exists for wild-caught live summer flounder (Paralichthys dentatus). An important component of accessing this market is the ability to hold wild-caught flounder in land based recirculating aquaculture systems (RAS). A major constraint to holding summer flounder in land-based RAS is fish mortalities associated with the ectoparasite argulus spp. Very little information exists that provides a live flounder holding facility with FDA approved treatment options to prevent introduction of argulus from wild caught fish into holding systems. The project objective was to test the available FDA approved chemotherapeutics for treatment of ectoparasite infections in summer flounder. To validate treatment efficacy, 8 infected individuals were treated with bath treatments according to maximum recommended doses (250ppm formalin for 60 m; 200 ppm hydrogen peroxide for 30 m; and freshwater for 20 m) and then transferred to separate RAS to monitor for two weeks. Daily observations were made to determine if treatments were lethal to the attached argulus. Following the two week monitoring period, fish from all treatments showed no sign of a reduction in attached argulus. According to the findings of this study, there are currently no FDA approved treatments for argulus infections on summer flounder. It is recommended that a live wild flounder holding facility visually inspect all incoming fish for the presence of argulus and maintain fish in a quarantine system prior to holding in a RAS

A Multi-user virtual environment for building higher order inquiry skills in science

This study investigated novel pedagogies for helping teachers infuse inquiry into a standards-based science curriculum. Using a Multi-User Virtual Environment(MUVE) as a pedagogical vehicle, teams of middle school students collaboratively solved problems around disease in a virtual town called River City. Students interacted with “avatars” of other students, digital artifacts, and computer-based “agents” acting as mentors and colleagues in a virtual community of practice set during the time period when bacteria was just being discovered. This paper describes the results from three implementations of the River City virtual environment in 2004 with approximately 2000 students from geographical diverse urban areas. Results indicate that students were able to conduct inquiry in virtual worlds and were motivated by that process. However, results from assessments vary depending on assessment strategy employed

Absence of p53 in Clara cells favours multinucleation and loss of cell cycle arrest

BACKGROUND: The p53 oncosuppressor protein is a critical mediator of the response to injury in mammalian cells and is mutationally inactivated in the majority of lung malignancies. In this analysis, the effects of p53-deficiency were investigated in short-term primary cultures of murine bronchiolar Clara cells. Clara cells, isolated from gene-targeted p53-deficient mice, were compared to cells derived from wild type littermates. RESULTS: p53 null cultures displayed abnormal morphology; specifically, a high incidence of multinucleation, which increased with time in culture. Multinucleated cells were proficient in S phase DNA synthesis, as determined by BrdU incorporation. However, multinucleation did not reflect altered rates of S phase synthesis, which were similar between wild type and p53-/- cultures. Nucleation defects in p53-/- Clara cells associated with increased centrosome number, as determined by confocal microscopy of pericentrin-stained cultures, and may highlight a novel role of p53 in preserving genomic integrity in lung epithelial cells. Effects of p53-deficiency were also studied following exposure to DNA damage. A p53-dependent reduction in the BrdU index was observed in Clara cells following ionizing radiation. The reduction in BrdU index in wild type cells displayed serum-dependency, and occurred only in the absence of serum. Taken together, these findings demonstrate that in murine primary Clara cell culture, cell cycle arrest is a p53-mediated response to DNA damage, and that extracellular factors, such as serum, influence this response. CONCLUSION: These findings highlight functions of wild type p53 protein in bipolar spindle formation, centrosome regulation, and growth control in bronchiolar Clara cells