In situ targeted activation of an anticancer agent using ultrasound-triggered release of composite droplets

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Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis

International audienceThe molecular mechanisms governing the formation of lymphatic vasculature are notyet well understood. Pannexins are transmembrane proteins that form channels which allow fordiffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol.The expression and function of pannexins in blood vessels have been studied in the last few decades.Meanwhile, no studies have been conducted to evaluate the role of pannexins during humanlymphatic vessel formation. Here we show, using primary human dermal lymphatic endothelial cells(HDLECs), pharmacological tools (probenecid, Brilliant Blue FCF, mimetic peptides [10Panx]) andsiRNA-mediated knockdown that Pannexin-1 is necessary for capillary tube formation on Matrigeland for VEGF-C-induced invasion. These results newly identify Pannexin-1 as a protein highlyexpressed in HDLECs and its requirement during in vitro lymphangiogenesis

Cell–cell interactions via non-covalent click chemistry

International audienceMetabolic glycoengineering with unnatural sugars became a valuable tool for introducing recognition markers on the cell membranes via bioorthogonal chemistry. By using this strategy, we functionalized the surface of tumor and T cells using complementary artificial markers based on both β-cyclodextrins (β-CDs) and adamantyl trimers, respectively. Once tied on cell surfaces, the artificial markers induced cell–cell adhesion through non-covalent click chemistry. These unnatural interactions between A459 lung tumor cells and Jurkat T cells triggered the activation of natural killer (NK) cells thanks to the increased production of interleukin-2 (IL-2) in the vicinity of cancer cells, leading ultimately to their cytolysis. The ready-to-use surface markers designed in this study can be easily inserted on the membrane of a wide range of cells previously submitted to metabolic glycoengineering, thereby offering a simple way to investigate and manipulate intercellular interactions

A dendritic β-galactosidase-responsive folate-monomethylauristatin E conjugate.

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A β-glucuronidase-responsive albumin-binding prodrug for potential selective kinase inhibitor-based cancer chemotherapy

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Down-regulation of connexin43 expression reveals the involvement of caveolin-1 containing lipid rafts in human U251 glioblastoma cell invasion

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TCA precipitation and ethanol/HCl single-step purification evaluation: One-dimensional gel electrophoresis, bradford assays, spectrofluorometry and Raman spectroscopy data on HSA, Rnase, lysozyme - Mascots and Skyline data

The data presented here are related to the research paper entitled “Study of a Novel Agent for TCA Precipitated Proteins Washing - Comprehensive Insights into the Role of Ethanol/HCl on Molten Globule State by Multi-Spectroscopic Analyses” (Eddhif et al., submitted for publication) [1]. The suitability of ethanol/HCl for the washing of TCA-precipitated proteins was first investigated on standard solution of HSA, cellulase, ribonuclease and lysozyme. Recoveries were assessed by one-dimensional gel electrophoresis, Bradford assays and UPLC-HRMS. The mechanistic that triggers protein conformational changes at each purification stage was then investigated by Raman spectroscopy and spectrofluorometry. Finally, the efficiency of the method was evaluated on three different complex samples (mouse liver, river biofilm, loamy soil surface). Proteins profiling was assessed by gel electrophoresis and by UPLC-HRMS

beta-Glucuronidase-responsive prodrugs for selective cancer chemotherapy: An update

International audienceThe design of novel antitumor agents allowing the destruction of malignant cells while sparing healthy tissues is one of the major challenges in medicinal chemistry. In this context, the use of non-toxic prodrugs programmed to be selectively activated by beta-glucuronidase present at high concentration in the microenvironment of most solid tumors has attracted considerable attention. This review summarizes the major progresses that have been realized in this field over the past ten years. This includes the new prodrugs that have been designed to target a wide variety of anticancer drugs, the prodrugs employed in the course of a combined therapy, the dendritic glucuronide prodrugs and the concept of beta-glucuronidase-responsive albumin binding prodrugs

Role of gap junctional intercellular communication in lymphangiogenesis and in prostate cancer cell adhesion to lymphatic endothelium

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Study of a novel agent for TCA precipitated proteins washing - comprehensive insights into the role of ethanol/HCl on molten globule state by multi-spectroscopic analyses

International audienc