Journey to the heart of macrophages: the delicate relationship between HIV-1 and a multifaceted cell type

International audienceCells of the monocyte-macrophage lineage play multiple roles during the infection of primate lentiviruses serving as reservoirs for viral production or as vectors for viral spread to other cells and tissues. The human immunodeficiency type I virus is not only capable of establishing such complex and dynamic relations with this cell type, but is also able to modulate their physiology and behavior, thus shaping ensuing cellular immune responses. In this issue of Retrovirology, a series of reviews explores the multiple manners in which the virus and cells belonging to the monocyte-macrophage lineage interact and affect each other

Multicomponent reactions

Multicomponent Reactions appear to be ideal for any form of synthesis, because of their numerous advantages in terms of sustainability and selectivity in building up complex molecular architectures, with high molecular diversity. This Special Issue collects seven contributions which expand our knowledge about Multicomponent Reactions, providing a good overview about innovative reactivities and applications

Dog Owners’ Interaction Styles: Their Components and Associations with Reactions of Pet Dogs to a Social Threat

The bond dogs develop with their owner received increased attention in the last years but no study aimed at characterizing the way in which owners interact with their dogs in their daily life and how this might influence dog behavior. In order to examine how dog owners interact with their dogs, we first analyzed the behavior of 220 dog owners in 8 different standardized situations involving the owner-dog dyad. We extracted 3 behavioral factors related to Owner Warmth, Owner Social Support and Owner Control. Further, we investigated whether owner personality, gender and age are associated with these three factors. Results indicated that older owners scored lower in Owner Warmth and in Owner Social Support and higher in Owner Control than younger owners. Furthermore, owners scoring high in Owner Control scored lower in the personality trait Openness and owners scoring high in Owner Social Support scored lower in the personality trait Conscientiousness. Finally, we also analyzed whether the dogs´ reaction to an unfamiliar woman’s threatening approach was associated with the owners’ interaction styles. Results showed that dogs that searched for proximity of their owners during the threatening situation had owners scoring higher in Owner Warmth, as compared to dogs that reacted more autonomously, approaching the unfamiliar experimenter. Analogies between dog-owner interaction styles and human parenting styles are discussed considering the implications of the present findings for human social psychology as well as the practical relevance for dog welfare and human safety

Prediction of turbulence control for arbitrary periodic spanwise wall movement

In order to generalize the well-known spanwise-oscillating-wall technique for drag reduction, non-sinusoidal oscillations of a solid wall are considered as a means to alter the skin-friction drag in a turbulent channel flow. A series of Direct Numerical Simulations is conducted to evaluate the control performance of nine different temporal waveforms, in addition to the usual sinusoid, systematically changing the wave amplitude and the period for each waveform. The turbulent average spanwise motion is found to coincide with the laminar Stokes solution that is constructed, for the generic waveform, through harmonic superposition. This allows us to define and compute, for each waveform, a new penetration depth of the Stokes layer which correlates with the amount of turbulent drag reduction, and eventually to predict both turbulent drag reduction and net energy saving rate for arbitrary waveforms. Among the waveforms considered, the maximum net energy saving rate is obtained by the sinusoidal wave at its optimal amplitude and period. However, the sinusoid is not the best waveform at every point in the parameter space. Our predictive tool offers simple guidelines to design waveforms that outperform the sinusoid for given (suboptimal) amplitude and period of oscillation. This is potentially interesting in view of applications, where physical limitations often preclude the actuator to reach its optimal operating conditions

The Inside Out of Lentiviral Vectors

Lentiviruses induce a wide variety of pathologies in different animal species. A common feature of the replicative cycle of these viruses is their ability to target non-dividing cells, a property that constitutes an extremely attractive asset in gene therapy. In this review, we shall describe the main basic aspects of the virology of lentiviruses that were exploited to obtain efficient gene transfer vectors. In addition, we shall discuss some of the hurdles that oppose the efficient genetic modification mediated by lentiviral vectors and the strategies that are being developed to circumvent them

Numerical experiments on turbulent entrainment and mixing of scalars

Numerical experiments on the turbulent entrainment and mixing of scalars in a incompressible flow have been performed. These simulations are based on a scale decomposition of the velocity field, thus allowing the establishment from a dynamic point of view of the evolution of scalar fields under the separate action of large-scale coherent motions and small-scale fluctuations. The turbulent spectrum can be split into active and inactive flow structures. The large-scale engulfment phenomena actively prescribe the mixing velocity by amplifying inertial fluxes and by setting the area and the fluctuating geometry of the scalar interface. On the contrary, small-scale isotropic nibbling phenomena are essentially inactive in the mixing process. It is found that the inertial mechanisms initiate the process of entrainment at large scales to be finally processed by scalar diffusion at the molecular level. This last stage does not prescribe the amount of mixing but adapts itself to the conditions imposed by the coherent anisotropic motion at large scales. The present results may have strong repercussions for the theoretical approach to scalar mixing, as anticipated here by simple heuristic arguments which are shown able to reveal the rich dynamics of the process. Interesting repercussions are also envisaged for turbulence closures, in particular for large-eddy simulation approaches where only the large scales of the velocity field are resolved

Numerical experiments on scalar transport and mixing in turbulent boundary layers

In this work, we present numerical experiments aimed at dynamically establishing the separate role of the inner and outer cycles on the scalar transport in the configuration of a temporally evolving boundary layer. The experiments are based on the study of the evolution of passive scalars driven by velocity fields where inner and outer cycles are alternately suppressed. Two different approaches are implemented. In the first, the discrimination between inner and outer cycle activities is based on the scale dimension of the involved motions. The second instead, discriminates on the basis of the distance from the wall of the turbulent motions. The two approaches depict the same scenario. Both the inner and outer cycles appear to be autonomous and, in a sense, independent, since their dynamics remain qualitatively unaltered despite facing two different conditions. The outer cycle faces a free boundary at the top and simply rescales according to what is supplied by the inner cycle. The inner cycle, on the other hand, resides between the wall and the outer region. As a result, the reduction of the scalar fluxes in the outer region due to the suppression of the outer cycle causes a damping in the near-wall region activities

On wind-wave interaction phenomena at low Reynolds numbers

After decades of research efforts, wind-wave interaction mechanisms have been recognized as extremely elusive. The reason is the complex nature of the problem, which combines complex coupling mechanisms between turbulent wind and water waves with the presence of multiple governing parameters, such as the friction Reynolds number of the wind, the water depth and the wind fetch. As shown unequivocally here, the use of suitable flow settings allows us to reduce the complex problem of wind-wave interaction to its essential features, mainly as a function of the sole friction Reynolds number of the wind. The resulting numerical solution allows us to study the interactions between water and air layers with their own fluid properties, and to unveil very interesting features, such as an oblique wave pattern travelling upstream and a wave-induced Stokes sublayer. The latter is responsible for a drag reduction mechanism in the turbulent wind. Despite the simulated flow conditions being far from the intense events occurring at the ocean-atmosphere interface, the basic flow phenomena unveiled here may explain some experimental evidence in wind-wave problems. Among other things, the wave-induced Stokes sublayer may shed light on the large scatter of the drag coefficient data in field measurements where swell waves of arbitrary directions are often present. Hence the present results and the developed approach pave the way for the understanding and modelling of the surface fluxes at the ocean-atmosphere interface, which are of overwhelming importance for climate science

Screening, isolation, and purification of active compounds against Klebsiella pneumoniae

The spread of multi-resistant bacteria is a contemporary real threat that constitutes a public health risk. Resistance to antibiotics has increased progressively since their discovery due to their misuse and abuse. Therefore, finding new antimicrobial compounds has become a worldwide task.By screening a culture collection, this study aimed to find bacteria and fungi able to inhibit the growth of Klebsiella pneumoniae. Replica plating, SPE-X columns, disk- diffusion assays, and agar-well diffusion assays are some of the techniques that have been used to isolate and purify the active compounds.Of all microorganisms tested, Epicoccum nigrum (MES 1587) showed the most promising results. It was able to inhibit not only K. pneumoniae, but also S. aureus, P. aeruginosa, B. subtilis, E. faecalis, and E. coli in both disk-diffusion assays and agar- well diffusion assays. It was also shown that its inhibition started upon 8 days of culture and increased over time.Regarding the rest of the microorganisms that inhibited K. pneumoniae in replica plating, it was impossible to reproduce the positive results after SPE-X purification.<br /