El comerç de mascotes exòtiques : aspectes legals i ètics /

Treball presentat a l'assignatura de Deontologia i Veterinària Legal (21223

Intracellular Ruthenium‐Promoted (2+2+2) Cycloadditions

This is the peer reviewed version of the following article: J. Miguel-Ávila, M. Tomás-Gamasa, J. L. Mascareñas, Angew. Chem. Int. Ed. 2020, 59, 17628, which has been published in final form at https://doi.org/10.1002/anie.202006689. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsMetal‐mediated intracellular reactions are becoming invaluable tools in chemical and cell biology, and hold promise for strongly impacting the field of biomedicine. Most of the reactions reported so far involve either uncaging or redox processes. Demonstrated here for the first time is the viability of performing multicomponent alkyne cycloaromatizations inside live mammalian cells using ruthenium catalysts. Both fully intramolecular and intermolecular cycloadditions of diynes with alkynes are feasible, the latter providing an intracellular synthesis of appealing anthraquinones. The power of the approach is further demonstrated by generating anthraquinone AIEgens (AIE=aggregation induced emission) that otherwise do not go inside cells, and by modifying the intracellular distribution of the products by simply varying the type of ruthenium complexThis work has received financial support from the Spanish Government (SAF2016‐76689‐R, ORFEO‐CINQA network CTQ2016‐81797‐REDC) the Consellería de Cultura, Educación e Ordenación Universitaria (2015‐CP082, ED431C‐2017/19 and Centro Singular de Investigación de Galicia Accreditation 2019–2022, ED431G 2019/03), the European Union (European Regional Development Fund‐ERDF corresponding to the multiannual financial framework 2014–2020), and the European Research Council (Advanced Grant No. 340055). J.M.Á. thanks the Ministerio de Educación, Cultura y Deporte for the FPU fellowship (FPU16/00711) and M.T.G. thanks the financial support from the Agencia Estatal de Investigación (RTI2018‐093813‐J‐I00)S

Bioimpedance Technique for Point-of-Care Devices Relying on Disposable Label-Free Sensors – An Anemia Detection Case

In this chapter, the development of a point-of-care device for bio-medical applications has been discussed. Our main objective is to research new electronic solutions for the detection, quantification, and monitoring of important biological agents in medical environments. The proposed systems and technologies rely on label-free disposable sensors, with portable electronics for user-friendly, low-cost solutions for medical disease diagnosis, monitoring, and treatment. In this chapter, we will focus on a specific point-of-care device for cellular analysis, applied to the case of anemia detection and monitoring. The methodology used for anemia monitoring is based on hematocrit measurement directly from whole blood samples by means of impedance analysis. The designed device is based on straightforward electronic standards for low power consumption and low-cost disposable sensor for low volume samples, resulting in a robust and low power consumption device for portable monitoring purposes of anemia. The device has been validated through different whole blood samples to prove the response, effectiveness, and robustness to detect anemia

La autoeficacia docente e investigadora del profesorado universitario: relación con su estilo docente e influencia en sus concepciones sobre el nexo docencia-investigación

The aim of this paper was to explore whether self-efficacy as teacher and researcher perceived by university lecturers was related to teaching styles and approaches. Two instruments were administered to 259 teachers at the University of Girona (Spain), who were active researchers and had a teaching workload of 9 ECTS or above. The results showed that self-efficacy as a researcher was the variable which yielded most differences. The higher the self-efficacy of the researcher, the stronger the belief that teaching was guided by research. Certain beliefs about the teaching-research nexus were very different according to the degree of self-efficacy as a researcher, while there were fewer differences in terms of gender and field of study.El objetivo de este trabajo es observar si la autoeficacia como profesor e investigador percibida por los profesores universitarios está relacionada con sus estilos o enfoques de enseñar. Se aplicaron dos instrumentos a 259 profesores de la Universidad de Girona con dedicación docente de 9 o más créditos y a miembros activos en tareas de investigación.En conclusión la variable autoeficacia como investigador es la variable que genera más diferencias. Los resultados indican que cuanto mayor es la autoeficacia como investigador, más fuerte es la convicción de que la investigación guía la enseñanza. Ciertas  convicciones sobre el nexo docencia-investigación son muy diferenciadas según  la autoeficacia investigadora  y existen menos diferencias en función de las variables género y ámbito de estudio

The role of red blood cell exchange for severe imported malaria in the artesunate era: a retrospective cohort study in a referral centre

BACKGROUND: Intravenous artesunate has replaced quinine as the first-line therapy for severe imported malaria, given its anti-malarial superiority shown in clinical trials conducted in endemic countries. Evidence for red blood cell (RBC) exchange in patients with severe malaria treated with artesunate is lacking. This retrospective cohort study describes the experience at Hospital Clinic of Barcelona with the use of artesunate for severe malaria and the joint use of RBC exchange in selected cases. METHODS: Patients treated for severe malaria at Hospital Clinic of Barcelona between August 2013 and January 2015 were included in this retrospective study. Severe malaria was defined according to WHO criteria. Data were extracted from electronic hospital records. A log-linear mixed model approach was used to estimate parasite clearance times. RESULTS: Within the study period, 42 patients were diagnosed of malaria at this centre, of which 38 had Plasmodium falciparum (90.5 %). Sixteen patients (42 %) had severe malaria cases and were treated with intravenous artesunate. Four patients underwent RBC exchange within a period of 15 h after the first dose of artesunate (range 9-21 h). The procedure lasted a median of 2 h (IQR 1.8-2 h), using a median of 12 (IQR 11-14) units of packed RBCs to replace a median of 3794 ml (IQR 2977-4343). The technique was well-tolerated without haemodynamic complications. There were no deaths. The regression model showed an estimated time to 95 % decay of 21.6 h (95 % CI 17.3-28.8). When assessing effect modification by RBC exchange, there was no difference in the parasite elimination rate (p = 0.286). DISCUSSION AND CONCLUSION: In this study RBC exchange failed to show benefits in terms of parasite clearance probably due to the small number of patients analysed. The evidence for exchange transfusion remains limited

Real-world effectiveness of caplacizumab vs the standard of care in immune thrombotic thrombocytopenic purpura

Effectiveness; Caplacizumab; Immune thrombotic thrombocytopenic purpuraEficàcia; Caplacizumab; Púrpura trombocitopènica trombòtica immunitàriaEficacia; Caplacizumab; Púrpura trombocitopénica trombótica inmunitariaImmune thrombotic thrombocytopenic purpura (iTTP) is a thrombotic microangiopathy caused by anti-ADAMTS13 antibodies. Caplacizumab is approved for adults with an acute episode of iTTP in conjunction with plasma exchange (PEX) and immunosuppression. The objective of this study was to analyze and compare the safety and efficacy of caplacizumab vs the standard of care and assess the effect of the concomitant use of rituximab. A retrospective study from the Spanish TTP Registry of patients treated with caplacizumab vs those who did not receive it was conducted. A total of 155 patients with iTTP (77 caplacizumab, 78 no caplacizumab) were included. Patients initially treated with caplacizumab had fewer exacerbations (4.5% vs 20.5%; P < .05) and less refractoriness (4.5% vs 14.1%; P < .05) than those who were not treated. Time to clinical response was shorter when caplacizumab was used as initial treatment vs caplacizumab used after refractoriness or exacerbation. The multivariate analysis showed that its use in the first 3 days after PEX was associated with a lower number of PEX (odds ratio, 7.5; CI, 2.3-12.7; P < .05) and days of hospitalization (odds ratio, 11.2; CI, 5.6-16.9; P < .001) compared with standard therapy. There was no difference in time to clinical remission in patients treated with caplacizumab compared with the use of rituximab. No severe adverse event was described in the caplacizumab group. In summary, caplacizumab reduced exacerbations and refractoriness compared with standard of care regimens. When administered within the first 3 days after PEX, it also provided a faster clinical response, reducing hospitalization time and the need for PEX

Front microrheology of the non-Newtonian behavior of blood: scaling theory of erythrocyte aggregation by aging

We introduce a new framework to study the non-Newtonian behaviour of fluids at the microscale based on the analysis of front advancement. We apply this methodology to study the non-linear rheology of blood in microchannels. We carry out experiments in which the non-linear viscosity of blood samples is quantified at different haematocrits and ages. Under these conditions, blood exhibits a power-law dependence on the shear rate. In order to analyse our experimental data, we put forward a scaling theory which allows us to define an adhesion scaling number. This theory yields a scaling behaviour of the viscosity expressed as a function of the adhesion capillary number. By applying this scaling theory to samples of different ages, we are able to quantify how the characteristic adhesion energy varies as time progresses. This connection between microscopic and mesoscopic properties allows us to estimate quantitatively the change in the cell-cell adhesion energies as the sample age

Programa de col·laboració entre l’atenció a la salut mental i les addiccions, i l'atenció primària i comunitària

Salut mental; Addiccions; Atenció primàriaSalud mental; Adicciones; Atención primariaMental health; Addictions; Primary careAquest programa es basa, d’una banda, en el reforç i sistematització del treball conjunt entre els professionals de l’APiC i els de SMiA i, de l’altra, en el desplegament del model esglaonat d’atenció que consisteix en l’atribució de nivells assistencials diferenciats i procediments diagnòstics i terapèutics específics en funció de la gravetat clínica i la complexitat psicosocial de les persones ateses. Aquest model defineix què, qui i com s’atén en els diferents esglaons: promoció de la salut mental, prevenció de trastorns mentals en poblacions de risc, detecció precoç, diagnòstic àgil i tractament més eficient en cada cas

Adherence to antiretroviral therapy and the associated factors among people living with HIV/AIDS in Northern Peru : a cross-sectional study

Altres ajuts: This study was partially funded by a research Grant from the Fundació Autònoma Solidària (Resolució Convocatòria FSXXXIII-Peru); and the Oficina de Investigación y Creatividad Intelectual from the Universidad María Auxiliadora (OICI-001-2017).There are approximately 72,000 people living with HIV/AIDS (PLHIV) in Peru. Non-adherence to antiretroviral therapy (ART) is the most important factor for therapeutic failure and the development of resistance. Peru has achieved moderate progress in meeting the 90-90-90 targets, but only 60% of PLHIV receiving ART are virally suppressed. The purpose of this study was to understand ART adherence in the Peruvian context, including developing sociodemographic and clinical profiles, evaluating the clinical management strategies, and analyzing the relationships between the variables and adherence of PLHIV managed at a regional HIV clinic in Lambayeque Province (Northern Peru). This was a cross-sectional study with 180 PLHIV adults, non-randomly but consecutively selected with self-reported ART compliance (78.2% of the eligible population). The PLHIV profile (PLHIV-Pro) and the Simplified Medication Adherence Questionnaire (SMAQ) were used to collect sociodemographic information, clinical variables, and data specific to ART adherence. Descriptive analysis of sociodemographic and clinical characteristics was performed. Bivariate analysis was performed with the Mann-Whitney test, Chi square test, and Yates correction. The 180 PLHIV sample included 78.9% men, 49.4% heterosexual, 45% with a detectable HIV-1 viral load less than 40 copies/ml, 58.3% not consistently adherent, and only 26.1% receiving Tenofovir + Lamivudine + Efavirenz. Risk factors significant for non-adherence included concurrent tuberculosis, discomfort with the ART regime, and previous pauses in ART. Multivariate analysis of nested models indicated having children is a protector factor for adherence. Self-reported adherence appeared to be low and the use of first-line therapy is not being prescribed homogeneously. Factors associated with nonadherence are both medical and behavioral, such as having tuberculosis, pausing ART, or experiencing discomfort with ART. The Peruvian government needs to update national technical standards, monitor medication availability, and provide education to health care professionals in alignment with evidence-based guidelines and international recommendations. Instruments to measure adherence need to be developed and evaluated for use in Latin America