The effect of sample properties on the electron velocity in quantum Hall bars

We report on our theoretical investigation of the effects of the confining potential profile and sample size on the electron velocity distribution in (narrow) quantum-Hall systems. The electrostatic properties of the electron system are obtained by the Thomas-Fermi-Poisson nonlinear screening theory. The electron velocity distribution as a function of the lateral coordinate is obtained from the slope of the screened potential at the Fermi level and within the incompressible strips (ISs). We compare our findings with the recent experiments.Comment: 8 pages, 6 figure

Alterations of T cell activation signalling and cytokine production by postmenopausal estrogen levels

<p>Abstract</p> <p>Background</p> <p>Immunosenescence is an age-associated disorder occurring primarily in T cell compartments, including altered subset composition, functions, and activation. In women, evidence implicates diminished estrogen in the postmenopausal period as a contributing factor to diminished T cell responsiveness. Since hypoestrogenism is present in postmenopausal women, our objective focused on whether T cell activation, defined as signalling molecule expressions and activation, and function, identified as IL-2 production, were affected by low estrogen.</p> <p>Methods</p> <p>Using Jurkat 6.1 T cells, consequences of 4 pg/ml (corresponding to postmenopausal levels) or 40 pg/ml (premenopausal levels) of estradiol (E₂) were analyzed on signalling proteins, CD3-zeta, JAK2, and JAK3, determined by Western immunoblotting. These consequences were correlated with corresponding gene expressions, quantified by real time-polymerase chain reaction. Tyrosine phosphorylation of CD3-zeta was defined by immunoprecipitation and western immunoblotting following activation by T cell receptor (TcR) cross-linking. CD3-zeta expression and modulation was also confirmed in T cells from pre- and postmenopausal women. To assess functional consequences, IL-2 production, induced by PMA and ionomycin, was determined using enzyme-linked immunosorbent spot assay (ELISpot).</p> <p>Results</p> <p>At 40 pg/ml E₂, the level of signalling protein CD3-zeta was elevated 1.57-fold, compared with cells exposed to 4 pg/ml E₂. The CD3-zeta proteins also exhibited altered levels of activation-induced phosphorylation in the presence of 40 pg/ml E₂versus 4 pg/ml: 23 kD phosphorylated form increased 2.64-fold and the 21 kD form was elevated 2.95-fold. Examination of kinases associated with activation signalling also demonstrated that, in the presence of 40 pg/ml E₂, JAK2 protein expression was increased 1.64-fold (p < 0.001) and JAK3 enhanced 1.79-fold (p < 0.001) compared to 4 pg/ml. mRNA levels for CD3-zeta, JAK2, and JAK3 were significantly increased following exposure to 40 pg/ml E₂(2.39, 2.01, and 2.21 fold, respectively) versus 4 pg/ml. These findings were confirmed in vivo, since T cells from postmenopausal women exhibited 7.2-fold diminished CD3-zeta expression, compared to pre-menopausal controls and this expression was elevated 3.8-fold by addition of 40 pg/ml E₂. Functionally, Jurkat cells exposed to 40 pg/ml E₂and activated exhibited significantly elevated numbers of IL-2 producing colonies compared to 4 pg/ml (75.3 ± 2.2 versus 55.7 ± 2.1 colonies, p < 0.0001).</p> <p>Conclusion</p> <p>Jurkat T cells exposed to 4 pg/ml E₂expressed significantly diminished activation signalling proteins, correlating with reduced IL-2 production. Lower signalling protein levels appear to result from decreased CD3-zeta, JAK2, and JAK3 gene expressions. These findings may provide a molecular basis for immunosenescence associated with the postmenopausal state.</p

The effect of long-term feeding of conjugated linoleic acid on fertility in Japanese quail

The objective of this study was to determine the effects of the long-term feeding of conjugated linoleic acid (CLA) on laying hen performance, egg fertility and hatchability of fertile eggs of Japanese quail (Coturnix coturnix japonica). One hundred and sixty 7-day old Japanese quail chicks were randomly assigned to four groups (40 birds per group) and fed for five weeks on a commercial diet supplemented with either 0.5% hazelnut (Group A), 0.5% sunflower oil (Group B), 0.25% CLA (Group C) or 0.5% CLA (Group D). The birds received water and feed ad libitum during the study. At the end of the five weeks the birds were sexed, and 21 females and seven males from each group were selected. For a further six weeks these selected birds were fed a layer diet supplemented with their respective sources of oil. Eggs were collected daily and incubated weekly. The fertility rate was determined by visual inspection at the end of the incubation period. Dietary CLA at the inclusion level of 0.5%, but not at 0.25%, caused a significant decrease in percentage hatchability of the fertile eggs compared to Groups A and B. Hatchability of fertile eggs from Groups A, B, C and D was 74, 80, 72 and 70, respectively. Fertility rates between Groups A, B, C and D did not differ significantly, and were 64.4, 56.4, 66.0 and 62.8%, respectively. Hen-day egg production of Groups A, C and D were similar, but in weeks 3, 4, 5 and 6, hens in Group B had a significantly lower hen-day egg production (%) compared to Group A. Also, in weeks 3, 4, 5 and 6, egg size in Group B was significantly lower than that in the other groups. From this study it is concluded that dietary CLA at inclusion levels of 0.25% or 0.5% did not influence egg fertility, egg weight and egg production in Japanese quail. South African Journal of Animal Science Vol. 36(2) 2006: 99-10

The effects of community interventions on unplanned healthcare use in patients with multimorbidity: a systematic review

OBJECTIVES: To summarise the impact of community-based interventions for multimorbid patients on unplanned healthcare use. The prevalence of multimorbidity (co-existence of multiple chronic conditions) is rapidly increasing and affects one-third of the global population. Patients with multimorbidity have complex healthcare needs and greater unplanned healthcare usage. Community-based interventions allow for continued care of patients outside hospitals, but few studies have explored the effects of these interventions on unplanned healthcare usage. DESIGN: A systematic review was conducted. MEDLINE, EMBASE, PsychINFO and Cochrane Library online databases were searched. Studies were screened and underwent risk of bias assessment. Data were synthesised using narrative synthesis. SETTING: Community-based interventions. PARTICIPANTS: Patients with multimorbidity. MAIN OUTCOME MEASURES: Unplanned healthcare usage. RESULTS: Thirteen studies, including a total of 6148 participants, were included. All included studies came from high-income settings and had elderly populations. All studies measured emergency department attendances as their primary outcome. Risk of bias was generally low. Most community interventions were multifaceted with emphasis on education, self-monitoring of symptoms and regular follow-ups. Four studies looked at improved care coordination, advance care planning and palliative care. All 13 studies found a decrease in emergency department visits post-intervention with risk reduction ranging from 0 (95% confidencec interval [CI]: -0.37 to 0.37) to 0.735 (95% CI: 0.688-0.785). CONCLUSIONS: Community-based interventions have potential to reduce emergency department visits in patients with multimorbidity. Identification of specific successful components of interventions was challenging given the overlaps between interventions. Policymakers should recognise the importance of community interventions and aim to integrate aspects of these into existing healthcare structures. Future research should investigate the impact of such interventions with broader participant characteristics

Selective Inhibition of Human AKR1B10 by n-Humulone, Adhumulone and Cohumulone Isolated from Humulus lupulus Extract

Hop-derived compounds have been subjected to numerous biomedical studies investigating their impact on a wide range of pathologies. Isomerised bitter acids (isoadhumulone, isocohumulone and isohumulone) from hops, used in the brewing process of beer, are known to inhibit members of the aldo-keto-reductase superfamily. Aldo-keto-reductase 1B10 (AKR1B10) is upregulated in various types of cancer and has been reported to promote carcinogenesis. Inhibition of AKR1B10 appears to be an attractive means to specifically treat RAS-dependent malignancies. However, the closely related reductases AKR1A1 and AKR1B1, which fulfil important roles in the detoxification of endogenous and xenobiotic carbonyl compounds oftentimes crossreact with inhibitors designed to target AKR1B10. Accordingly, there is an ongoing search for selective AKR1B10 inhibitors that do not interact with endogeneous AKR1A1 and AKR1B1-driven detoxification systems. In this study, unisomerised α-acids (adhumulone, cohumulone and n-humulone) were separated and tested for their inhibitory potential on AKR1A1, AKR1B1 and AKR1B10. Also AKR1B10-mediated farnesal reduction was effectively inhibited by α-acid congeners with Ki-values ranging from 16.79 ± 1.33 µM (adhumulone) to 3.94 ± 0.33 µM (n-humulone). Overall, α-acids showed a strong inhibition with selectivity (115⁻137 fold) for AKR1B10. The results presented herein characterise hop-derived α-acids as a promising basis for the development of novel and selective AKR1B10-inhibitors

Structural tuning of color chromaticity through nonradiative energy transfer by interspacing CdTe nanocrystal monolayers

Cataloged from PDF version of article.We proposed and demonstrated architectural tuning of color chromaticity by controlling photoluminescence decay kinetics through nonradiative Forster resonance energy transfer in the heterostructure of layer-by-layer spaced CdTe nanocrystal (NC) solids. We achieved highly sensitive tuning by precisely adjusting the energy transfer efficiency from donor NCs to acceptor NCs via controlling interspacing between them at the nanoscale. By modifying decay lifetimes of donors from 12.05 to 2.96 ns and acceptors from 3.68 to 14.57 ns, we fine-tuned chromaticity coordinates from (x,y)=(0.575,0.424) to (0.632, 0.367). This structural adjustment enabled a postsynthesis color tuning capability, alternative or additive to using the size, shape, and composition of NCs

Betatrophin levels are related to the early histological findings in nonalcoholic fatty liver disease

Betatrophin, a liver hormone, regulates glucose and lipid metabolism. We investigated the betatrophin levels in nonalcoholic fatty liver disease (NAFLD) and searched for any relationship with histological severity and metabolic parameters. Fifty males with NAFLD [Nonalcoholic Steatohepati-tis (NASH) (n = 32); non-NASH (n = 18)] and 30 healthy controls were included. Plasma betatrophin was measured by ELISA method. Insulin sensitivity was assessed by HOMA-IR index. Histological features were scored by the semi quantitative classification and combined as the NAFLD activity score (NAS). Betatrophin levels in the non-NASH group were significantly higher than the controls. Betatrophin was positively correlated to the age, waist circumference, total cholesterol, triglycerides, LDL cholesterol, glucose, insulin, HOMA-IR index and gamma glutamyl transpeptidase levels, and negatively correlated to the steatosis and NAS. In the stepwise linear regression analysis, the triglyceride (β = 0.457, p &lt; 0.001), glucose (β = 0.281, p = 0.02) and NAS (β = −0.260, p = 0.03) were the independent determinants of betatrophin. Betatrophin levels are higher in the early stages of NAFLD and tend to decrease when the disease progresses. This could be an important preliminary mechanistic finding to explain the increased frequency of glucose intolerance during the course of NAFLD

Uptake of agroforestry-based crop management in the semi-arid Sahel – Analysis of joint decisions and adoption determinants.

Open Access JournalIntroduction: Agroforestry plays a vital role in maintaining and developing the resilience and productivity of farms and landscapes. Scientific evidence from the Sahel region suggests that integration of trees and shrubs has the potential to improve temperature and moisture levels whilst providing bio-based fertilizer that contributes to increased yields of annual crops. However, little is known about the factors that influence the diusion of agroforestry. This study examines joint decisions on the use of agroforestry alongside other complementary agricultural practices and disentangles agroforestry awareness fromadoption and disadoption decisions. Methods: Our analysis is based on a comprehensive farm-level dataset covering almost 3,000 farm households in Mali and Senegal. A large number of adoption determinants are utilized, with a special focus on information access, information flows and social groups. Results: The findings suggest that extension access and training participation boost awareness of agroforestry-based soil fertility management, while information provided by public extension, NGOs and community members is strongly associated with higher adoption intensity. In the analysis of disadoption, farmer-to-farmers exchange in the community was found to be a key factor in the decision to maintain agroforestry use. Membership in cooperatives and youth groups appear to have a favorable eect on awareness and adoption in Mali, but less so in the Senegalese case. Similarly, only results from Mali show that adoption of agroforestry is accompanied by the adoption of other sustainable intensification practices and lower use of synthetic pesticides. Discussion: We conclude that in order to support the transition to more widespread agroforestry-based soil fertility management, it is essential to strengthen public and NGO-based advisory systems that fully engage with local knowledge networks