A CASE WITH POSTCHEMOTHERAPY ERUPTIVE COMPOUND NEVUS

An 8-year-old male patient who had been diagnosed as acute lymphoblastic leukemia (ALL) 4 years ago and received 120 cures of chemotherapy presented at our clinic complaining of spots on his body 3 months after the end of chemotherapy. Anamnesis of the patient revealed that the lesions started 3 months after the last cure of chemotherapy on the abdomen and spread throughout the body. It was learnt that the number of spots increased very rapidly in a period of 2 months, and exceeded 100. A dermatological examination of the patient showed 142 hyperpigmented macules, the largest of which was 1 cm × 1 cm and the smallest was 0.2 cm × 0.2 cm in size, diffusely scattered to the skin and oral mucosa. When the biopsy material taken from the lesions was histopathologically examined, nest structures composed of nevus cells with oval nuclei and eosinophilic cytoplasm starting from the dermoepidermal junction and extending toward the lower epidermis were observed and found to be consistent with the compound nevus. We present this rare case who was diagnosed as “eruptive compound nevus” on the basis of clinical signs, ALL diagnosis, chemotherapy history and histopathologic evaluation

Comparing of 540 and 540E PTO Operational Characteristics of Tractors in Field Conditions

WOS: 000279315400005The objective of this study is to determine the operating characteristics of economical PTO operation (540E) in field conditions and to make a comparison with 540 PTO operation. For this purpose three different brands of tractors (JD 5625, NH TD85, MF 3085) having similar technical properties and three PTO driven agricultural machine (single disc fertilizer spreader, turbo atomizer and rotovator. In the experiments tractor ground speed, slip values, PTO torque, PTO power, fuel consumption per time, effective field capacity and fuel consumption per field parameters were determined for two PTO operations. According to the fuel consumption data obtained, the fuel saving ratios obtained with 540E operation varied between 14.69%-34.41% To for the tractor-machine interactions used. It was determined that the motor-PTO speed ratios of the tractors were effective on fuel saving ratios. In the field experiments made at a single gear level for each tractor, the tractor ground speed values decreased by ratios varying between 12.83%-22.60% for 540E PTO operations in comparison to 540 PTO operations. This result that is caused by running both PTO operations at different motor speeds also caused a decrease in the machine's effective field capacity values calculated for 540E operation. Taking into account all the measurement parameters, it was concluded that economical PTO operation would bring certain advantages in terms of fuel consumption for many PTO driven machines and that these advantages decreased depending on the increase in the machine power requirement. According to this result, if economical PTO operation is used together with the proper gear level and ground speed selections for the tractors, it can be suggested as an important alternative to the 540 operation for machines used in this study and also for those having similar capacities and properties

Brucella Melitensis Misidentified As Roseomonas Gilardii By Maldi-Tof Ms: Experience Of A Clinical Microbiology Laboratory

Misidentification of a blood culture isolate of Brucella melitensis as Roseomonas gilardii by the VITEK (R) MS (bioMerieux, Marcy l'Etoile, France) matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) microbial identification system is herein drawn attention. Blood and serum samples of a 42-year-old male with pre-diagnosed neurobrucellosis were evaluated in bacteriology and serology laboratories. Gram-negative coccobacilli from positive blood culture bottle were identified as R. gilardii by VITEK (R) MS, but it was not identified by VITEK (R) 2 Compact (bioMerieux, Marcy l'Etoile, France) MALDI-TOF MS microbial identification system. Serological tests were positive for brucellosis, so it was an important clue for the possibility of a misidentification. The isolated organism was confirmed as B. melitensis by polymerase chain reaction and biotyping in the National Reference Laboratory. Microbiology laboratories in countries where brucellosis is endemic must be careful while using commercial bacterial identification systems for identification of Brucella spp.WoSScopu

Accurate Diagnosis of Pseudomonas luteola in Routine Microbiology Laboratory: On the Occasion of Two Isolates

Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P.luteola infections. This report was aimed to draw attention to the accurate identification of P.luteola in clinical samples, upon the isolation and identification in two cases in the medical microbiology laboratory of a university hospital. In February 2011, a 66-year-old man, with chronic obstructive pulmonary disease, coronary artery disease and aplastic anemia, was admitted to our hospital due to progressive dyspnea. A chest tube was inserted on the 20th day of admission by the reason of recurrent pleural effusion. Staphylococcus aureus and a non-fermentative gram-negative bacillus (NFGNB) with wrinkled, sticky yellow colonies were isolated from the pleural fluid sample obtained on the 9th day following the insertion of the chest tube. In February 2012, a 7-year-old male cystic fibrosis patient who had no signs and symptoms of acute pulmonary exacerbation was admitted to the hospital for a routine control. This patient had chronic colonization with Pseudomonas aeruginosa and S.aureus and his sputum sample obtained at this visit revealed isolation of P.aeruginosa, S.aureus, Aspergillus fumigatus and a wrinkled, sticky yellow NFGNB. Both of these NFGNB were identified as P.luteola by the Phoenix automated microbial identification system (BD Diagnostics, USA). To evaluate the microbiological characteristics of these two isolates, the strains were further analysed by VITEK MS (bioMerieux, France) and Microflex LT mass spectrometer (Bruker Daltonics, Germany). Both of the MALDI-TOF-MS systems identified the isolates as P.luteola and 16S rRNA gene sequencing (ABI PRISM 3100, Applied Biosystems, USA) also confirmed the identification. The strains had wrinkled, sticky yellow colonies which were oxidase-negative, catalase-positive and non-fermentative. The Gram stained smears of the colonies revealed clusters of gramnegative bacilli probably embedded into a biofilm matrix. Since there are no accepted standards for testing the antibiotic susceptibility of P.luteola strains, the standards determined by CLSI for "other non-Enterobacteriaceae" (non-fermentative bacteria excluding P.aeruginosa, Acinetobacter spp., Burkholderia cepacia, B.mallei, B.pseudomallei and Stenotrophomonas maltophilia) were used for the susceptibility testing. Gradient MIC method (E-Test, bioMerieux, France) revealed that the isolates were susceptible to gentamicin, piperacillin-tazobactam, ceftazidime, cefepime, meropenem, colistin and levofloxacin. Accurate and prompt identification of P.luteola which is identified as a rare pathogen in serious cases is of critical importance since it has been suggested that this organism is likely to become more frequent as a nosocomial pathogen since the interventional processes increase in current medical practice. This report supported that Phoenix automated phenotypic identification system (BD Diagnostics, USA) and the two MALDI-TOF-MS based systems (VITEK MS and Bruker Microflex LT mass spectrometer) were successfull in the accurate identification of P.luteola

Epidemiological, Clinical, and Laboratory Features of Children With COVID-19 in Turkey

Objectives: The aim of this study is to identify the epidemiological, clinical, and laboratory features of coronavirus disease 2019 (COVID-19) in children