MOESM1 of SIRT1 ameliorates oxidative stress induced neural cell death and is down-regulated in Parkinson’s disease

Additional file 1: Figure S1. Expression of SIRT1 in toxin treated SH-SY5Y cells. SIRT1WT and SIRT1H363Y were over-expressed in SH-SY5Y cells and control cells were transfected with empty vector following which cells were treated with diquat (20 or 10 μM) or rotenone (20 or 0.5 μM) for 20 h. Cells were harvested and the samples were probed for SIRT1. Data are presented as fold- untreated (+SD) from three independent assays (n = 3) with comparison to GAPDH as a housekeeping control protein. ***p < 0.001 when compared to 0.2% PBS, one-way ANOVA (Bonferroni corrected), ###p < 0.001 when compared to empty vector treatment, two-way ANOVA (Bonferroni corrected). Images are representative blot of SIRT1 and GAPDH

Towards development and validation of an intraoperative assessment tool for robot-assisted radical prostatectomy training: results of a Delphi study

<div><p>ABSTRACT Introduction As urology training shifts toward competency-based frameworks, the need for tools for high stakes assessment of trainees is crucial. Validated assessment metrics are lacking for many robot-assisted radical prostatectomy (RARP). As it is quickly becoming the gold standard for treatment of localized prostate cancer, the development and validation of a RARP assessment tool for training is timely. Materials and methods We recruited 13 expert RARP surgeons from the United States and Canada to serve as our Delphi panel. Using an initial inventory developed via a modified Delphi process with urology residents, fellows, and staff at our institution, panelists iteratively rated each step and sub-step on a 5-point Likert scale of agreement for inclusion in the final assessment tool. Qualitative feedback was elicited for each item to determine proper step placement, wording, and suggestions. Results Panelist’s responses were compiled and the inventory was edited through three iterations, after which 100% consensus was achieved. The initial inventory steps were decreased by 13% and a skip pattern was incorporated. The final RARP stepwise inventory was comprised of 13 critical steps with 52 sub-steps. There was no attrition throughout the Delphi process. Conclusions Our Delphi study resulted in a comprehensive inventory of intraoperative RARP steps with excellent consensus. This final inventory will be used to develop a valid and psychometrically sound intraoperative assessment tool for use during RARP training and evaluation, with the aim of increasing competency of all trainees.</p></div

Supplementary table -Supplemental material for Sleep positioning systems for children and adults with a neurodisability: A systematic review

<p>Supplemental material, Supplementary table for Sleep positioning systems for children and adults with a neurodisability: A systematic review by Ginny Humphreys, Tanya King, Jo Jex, Morwenna Rogers, Sharon Blake, Jo Thompson-Coon and Christopher Morris in British Journal of Occupational Therapy</p

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis-2

Band (-), faintly visible band (+/-), visible band (+), strongly visible band (++), N = Not Done. β-actin served as the internal comparative control. The grey values of PCR products of each gene are analyzed by the AlphaEase 3 software and standardized according to β-actin in every sample. B: a, b, Daughter melanoma cell lines secrete SPP1 (osteopontin). The melanoma cell lysates and conditioned cell-free media was resolved by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with anti-SPP1 antibody. , Antibodies for DSC-3 (c), CLCA2 (d), PDGFRL (e) and α-tubulin (f) as an internal control. Lanes 1–3 are PCM and lanes 4–6 are MM cell lines.<p><b>Copyright information:</b></p><p>Taken from "The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis"</p><p>http://www.biomedcentral.com/1755-8794/1/13</p><p>BMC Medical Genomics 2008;1():13-13.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2408576.</p><p></p

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis-1

oncogenes (GDF15, SPP-1) in PCM (n = 7) and MM (n = 13) samples. Relative quantitation of target gene expression for each sample was determined using the equation 2, where GAPDH was used as the internal reference and normal skin as the calibrator. Values were Log base 10 transformed (y-axis) so that all values below zero represent a down-regulation in gene expression and values above zero represent an up-regulation in gene expression, compared to normal skin. B: Correlative microarray analysis of gene expression levels in primary and metastatic melanoma samples compared with normal skin. The statistical differences of gene expression between primary (PCM) and metastatic melanoma (MM) samples were analyzed by Wilcoxon's signed rank test; two-tailed significance level was set at α = 0.05. Compared to PCM samples (n = 7), the expression levels of 4 putative tumor suppressor genes (CST6, p < 0.0001; DSC3, p < 0.0001; PITX1, p = 0.0043, POU2F3, p < 0.0001) were significantly decreased in MM samples (n = 40), while the expression of putative oncogenes (GDF15, p = 0.0027; SPP1, p < 0.0001) were significantly increased in MM samples.<p><b>Copyright information:</b></p><p>Taken from "The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis"</p><p>http://www.biomedcentral.com/1755-8794/1/13</p><p>BMC Medical Genomics 2008;1():13-13.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2408576.</p><p></p

The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis-0

Measured utilizing Breslow's depth of invasion. All procured lymph node metastases were macroscopically involved, often completely replacing the entire lymph node parenchyma. Distant metastatic (subcutaneous and solid organ) melanoma often exhibited varying degrees of pigmentation, however, surrounding stroma was avoided in procurement of melanoma samples. B: A distinct change in the gene expression patterns is apparent within the comparative groups of thin/I.M. to thick PCM samples. Gene over-expression (upper graph) is evident at the I.M. thickness sample set, with an average Breslow's tumor thickness of 2.1 mm and 19 mm for thick melanomas. Contrary, there is a decrease in gene expression (lower graph) of the same set of genes, with a comparative difference in gene down-regulation evident at the same interphase of I.M. to thick PCM. Proceeding from left to right: normal skin, BCC, SCC, MIS, I.M., thick primary, metastatic melanoma (subcutaneous, lymph node and distant) and melanoma cell lines derived from patients with stage IV melanoma.<p><b>Copyright information:</b></p><p>Taken from "The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis"</p><p>http://www.biomedcentral.com/1755-8794/1/13</p><p>BMC Medical Genomics 2008;1():13-13.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2408576.</p><p></p

Are consumption of dairy products and physical activity independently related to bone mineral density of 6-year-old children? Longitudinal and cross-sectional analyses in a birth cohort from Brazil

Objective: To evaluate cross-sectional and longitudinal associations of consumption of dairy products and physical activity (PA) with bone mineral density (BMD). Design: Cohort study with children from the 2004 Pelotas (Brazil) Birth Cohort. Setting: Pelotas, a medium-sized Brazilian city. Subjects: The study started in 2004 and mothers/children were interviewed/measured periodically from birth to age 6 years. PA was measured by maternal proxy at 4 and 6 years and by accelerometry at 6 years. Consumption of dairy products was measured using 24 h food recall (at 4 years) and FFQ (at 6 years). Total-body and lumbar-spine BMD (g/cm2) were measured by dual-energy X-ray absorptiometry. Results: At 6 years, BMD was measured in 3444 children and 2636 children provided data on objectively measured PA by accelerometry. Consumption of dairy products at 4 years was associated with higher lumbar-spine BMD at 6 years in boys, while current consumption was positively associated with BMD in both sexes (P < 0·001). PA assessed by maternal report at 4 and 6 years of age was associated with higher BMD at 6 years in boys. PA assessed by accelerometry was positively related to total-body and lumbar-spine BMD in boys and lumbar-spine BMD in girls. We did not find evidence for an interaction between PA and consumption of dairy products on BMD. Conclusions: We observed positive and independent longitudinal and cross-sectional associations between consumption of dairy products and PA with BMD in the total body and at the lumbar spine in young children

Additional file 1: of Mitochondrial DNA point mutations and relative copy number in 1363 disease and control human brains

Supplementary Material. (DOCX 6597 kb