Tracking of synoptic weather systems in the Siberian Arctic and their impact on the Laptev Sea polynya

Synoptic weather systems in the Arctic are studied using a track algorithm based on a feature-tracking method. The tracking is performed with the 850 hPa relative vorticity field derived from the NCEP/NCAR reanalysis dataset for the winter seasons 1978-2007. A climatology of synoptic systems is calculated for the whole Arctic. A low cyclone track density is found for eastern Siberia and the Pacific part of the Arctic. Cyclonic and anticyclonic track density decreases from the first half to the second half of the 30-year investigation period in large parts of the Arctic. A detailed study is performed for the Laptev Sea area of the Siberian Arctic, whis is an active area for sea ice production in flaw polynyas. The reaction of the Laptev polynya system to tracks of cyclones and anticyclones is investigated by selecting favourate track directions. The Anabar-Lena Polynya is affected by cyclones moving eastwards across the Laptev Sea. These cyclones cause an opening of the polynya on the day before the cyclone passage and a closing on the day after. The West-New-Siberian Polynya (WNS) is affected by cyclone tracks moving northwards along the western flank of the Laptev Sea. The cyclones mainly have an opening impact one and two days before the cyclone passage. For the WNS polynya, anticyclones passing from the east have the largest effect

The effect of sodium thiosulfate on immune cell metabolism during porcine hemorrhage and resuscitation

Introduction Sodium thiosulfate (Na2S2O3), an H2S releasing agent, was shown to be organ-protective in experimental hemorrhage. Systemic inflammation activates immune cells, which in turn show cell type-specific metabolic plasticity with modifications of mitochondrial respiratory activity. Since H2S can dose-dependently stimulate or inhibit mitochondrial respiration, we investigated the effect of Na2S2O3 on immune cell metabolism in a blinded, randomized, controlled, long-term, porcine model of hemorrhage and resuscitation. For this purpose, we developed a Bayesian sampling-based model for 13C isotope metabolic flux analysis (MFA) utilizing 1,2-13C2-labeled glucose, 13C6-labeled glucose, and 13C5-labeled glutamine tracers. Methods After 3 h of hemorrhage, anesthetized and surgically instrumented swine underwent resuscitation up to a maximum of 68 h. At 2 h of shock, animals randomly received vehicle or Na2S2O3 (25 mg/kg/h for 2 h, thereafter 100 mg/kg/h until 24 h after shock). At three time points (prior to shock, 24 h post shock and 64 h post shock) peripheral blood mononuclear cells (PBMCs) and granulocytes were isolated from whole blood, and cells were investigated regarding mitochondrial oxygen consumption (high resolution respirometry), reactive oxygen species production (electron spin resonance) and fluxes within the metabolic network (stable isotope-based MFA). Results PBMCs showed significantly higher mitochondrial O2 uptake and lower O 2 • − production in comparison to granulocytes. We found that in response to Na2S2O3 administration, PBMCs but not granulocytes had an increased mitochondrial oxygen consumption combined with a transient reduction of the citrate synthase flux and an increase of acetyl-CoA channeled into other compartments, e.g., for lipid biogenesis. Conclusion In a porcine model of hemorrhage and resuscitation, Na2S2O3 administration led to increased mitochondrial oxygen consumption combined with stimulation of lipid biogenesis in PBMCs. In contrast, granulocytes remained unaffected. Granulocytes, on the other hand, remained unaffected. O 2 • − concentration in whole blood remained constant during shock and resuscitation, indicating a sufficient anti-oxidative capacity. Overall, our MFA model seems to be is a promising approach for investigating immunometabolism; especially when combined with complementary methods

Functional modeling of the craniospinal system for in-vitro parameter studies on the pathogenesis of NPH

Normal Pressure Hydrocephalus (NPH) has become a common disease in the elderly coming along with typical symptoms of dementia, gait ataxia and urinary incontinence, which make the differential diagnosis with other forms of dementia difficult. Furthermore the pathogenesis of NPH is still not understood. About 10% of all demented patients might be suffering from NPH [1]. Many hypotheses suggest that modified biomechanical boundary conditions affect the craniospinal dynamics inducing the pathogenesis of NPH. We present a novel approach for an in-vitro model of the craniospinal system to investigate important hydrodynamic influences on the system such as (dynamic) compliance of the vascular system and especially the spinal subarachnoid space (SAS) as well as reabsorption and hydrostatics. The experimental set-up enables the individual adjustment of relevant parameters for sensitivity analyses regarding the impact of resulting CSF dynamics on the pathogenesis of NPH