Larvae and relationships of epinepheline serranids (Teleostei: Percoidei)

Morphology is described for larvae of Diploprion bifasciatus, Belonoperca chabanaudi, Jeboehlkia gladifer, and Grammistes sexlineatus of the serranid subfamily Epinephelinae. Known larvae of all epinephelines are compared. Larval epinephelines differ in patterns of pigmentation, head spination, sequence of fin formation and morphology of elongate (often spectacularly so!) dorsal-fin spines. Relationships among epinepheline genera are investigated based on phylogenetic analysis of larval and adult morphology. Five monophyletic tribes are cladistically delineated, and relationships among tribes and among genera of the tribe Grammistini are hypothesized. Generic composition of tribes differs from Johnson\u27s (1983) classification only in the allocation of Jeboehlkia to the tribe Grammistini rather than the Liopropomini. Despite the presence of the ichthyotoxin grammistin in the Diploprionini and Grammistini, the latter is proposed to be the sister group of the Liopropomini. This hypothesis is based, in part, on previously unrecognized larval features. Larval morphology also provides convincing evidence of monophyly of the subfamily Epinephelinae, the clade comprising all epinepheline tribes except Niphonini, and the tribe Grammistini. Larval features provide the only evidence of a monophyletic Epinephelini and a monophyletic clade comprising the Diploprionini, Liopropomini and Grammistini; identification of larvae of more epinephelines is needed to test those hypotheses. Within the tribe Grammistini, Jeboehlkia gladifer is hypothesized to be the sister group of a natural assemblage comprising the former pseudogrammid genera (Aporops, Pseudogramma and Suttonia). The soapfishes (Grammistes, Grammistops, Pogonoperca and Rypticus) are not monophyletic, but form a series of sequential sister groups of Jeboehlkia, Aporops, Pseudogramma and Suttonia (the closest of these being Grammistops, followed by Rypticus, then Grammistes plus Pogonoperca). The absence in adult Jeboehlkia of several derived features shared by Grammistops, Aporops, Pseudogramma and Suttonia is incongruous with this hypothesis but may be attributable to paedomorphosis. The generic phylogeny of the Grammistini proposed herein emerges as the single most parsimonious hypothesis largely because of the method chosen for analyzing multistate characters. This study demonstrates that ontogeny is valuable in phylogenetic studies as a source of characters, means of assessing homology and aid to identifying heterochrony

CEJ Ankle Support Report

This document provides information about an ankle support device designed to allow for ankle dorsiflexion and plantar flexion. In this document the product’s specifications, objectives and expectations are outlined. Included in this document is background information, stage gate process, prototype design development and considerations, and qualification requirements

The 1967 Project

This program profile describes an intergenerational workshop focused on the 1967 Detroit Rebellion. The workshop was nested within a 15-week community-based intermediate composition course in which undergradu- ates interviewed older adults while older adults wrote personal narratives of their firsthand experiences during the rebellion. The workshop is an example of how intergenerational collaboration built around inquiry into historical events can be the basis for authentic community-university relationships

Natural variation in linalool metabolites: One genetic locus, many functions?

The ubiquitous volatile linalool is metabolized in plants to nonvolatile derivatives. We studied Nicotiana attenuata plants which naturally vary in (S)‐(+)‐linalool contents, and lines engineered to produce either (R)‐(‐)‐ or (S)‐(+)‐linalool. Only (S)‐(+)‐linalool production was associated with slower growth of a generalist herbivore, and a large fraction was present as nonvolatile derivatives. We found that variation in volatile linalool and its nonvolatile glycosides mapped to the same genetic locus which harbored the biosynthetic gene, NaLIS, but that free linalool varied more in environmental responses. This study reveals how (S)‐(+)‐linalool and conjugates differ in their regulation and possible functions in resistance

Games for civic learning: A conceptual framework and agenda for research and design

Scholars, educators, and media designers are increasingly interested in whether and how digital games might contribute to civic learning. However, there are three main barriers to advancing understanding of games’ potential for civic education: the current practices of formal schooling, a dearth of evidence about what kinds of games best inspire learning about public life, and divergent paradigms of civic engagement. In response, this article develops a conceptual framework for how games might foster civic learning of many kinds. The authors hypothesize that the most effective games for civic learning will be those that best integrate game play and content, that help players make connections between their individual actions and larger social structures, and that link ethical and expedient reasoning. This framework suggests an agenda for game design and research that could illuminate whether and how games can be most fruitfully incorporated into training and education for democratic citizenship and civic leadership

Portrayals of information and communication technology on World Wide Web sites for girls

This study reports a content analysis of 35 World Wide Web sites that included in their mission the goal of engaging girls with information and communication technology (ICT). It finds that sites emphasize cultural and economic uses of ICT, doing little to foster civic applications that could empower girls as citizens of the information age. The study also finds that sites foster a narrow range of ICT proficiencies, focusing mostly on areas such as communication, in which girls have already achieved parity with boys. An examination of the role models portrayed in ICT occupations indicates that the sites show females mainly in elite technology jobs, reversing stereotypical mass media depictions of females in low-status roles in relation to ICT. Employing an original index of ICT knowledge and skills, the study finds that the sites that scored highest both on fostering comprehensive knowledge and skills as well as featuring civic content were general interest Web communities. Ownership (for-profit or not-for-profit) of sites was less important than editorial control: Sites that offered girls a place to contribute their own content were more likely to offer civic material and a broader range of ICT knowledge and skills. We conclude with recommendations for Web site designers to rethink their design strategies and their rationales for closing the gender gap in computing

Enhancing experiential learning in planning education through an online toolkit of resources

This paper reports on the development of an online toolkit of resources to support the use and assessment of experiential learning (EL) across planning programs. Planning graduates face diverse workplace demands with expectations to address the local and regional implications of global trends, integrate technological advances with existing planning processes, as well as interpret and integrate changing legislative and institutional arrangements. Planning education is about educators, practitioners and students coming together. Equipping students for increasing complexity and change requires planning educators to design programs that facilitate the learning of personal and professional skills and both broad and specialised planning knowledge. Planning practitioners, in partnership with educators, make further valuable contributions by providing a range of ‘real world’ learning experiences where students can directly develop new skills, knowledge and qualities. Experiential learning allows students to develop confidence within a safe environment through a series of activities and reflection that link theory to practice. However, developing EL learning outcomes, teaching activities and assessment can be demanding and time consuming for planning educators. In response, the Experiential Learning in Planning Education: Resources and Tools for Good Practice research project developed an online toolkit of case studies and associated resources to assist planning educators and practitioners in their application of EL. These case studies, developed by the partners in the project, explain the rationale of using particular EL activities based on a set of EL principles; how these activities are assessed; and reflections on how they could be improved for future use. The case studies also include numerous ‘how to’ resources, such as checklists and handy hints for organising activities, evaluation tools and examples of students’ work. Furthermore, the toolkit website is an interactive and ‘living’ repository where additional case studies by other EL users can be included to enhance the diversity and richness of the resources available. The toolkit is expected to particularly benefit new planning educators but should also be of interest to planning schools and practitioners around the globe who are endeavouring to facilitate students’ education in a rapidly changing world

A persistent major mutation in canonical jasmonate signaling is embedded in an herbivory-elicited gene network

When insect herbivores attack plants, elicitors from oral secretions and regurgitants (OS) enter wounds during feeding, eliciting defense responses. These generally require plant jasmonate (JA) signaling, specifically, a jasmonoyl-L-isoleucine (JA-Ile) burst, for their activation and are well studied in the native tobacco Nicotiana attenuata. We used intraspecific diversity captured in a 26-parent MAGIC population planted in nature and an updated genome assembly to impute natural variation in the OS-elicited JA-Ile burst linked to a mutation in the JA-Ile biosynthetic gene NaJAR4. Experiments revealed that NaJAR4 variants were associated with higher fitness in the absence of herbivores but compromised foliar defenses, with two NaJAR homologues (4 and 6) complementing each other spatially and temporally. From decade-long seed collections of natural populations, we uncovered enzymatically inactive variants occurring at variable frequencies, consistent with a balancing selection regime maintaining variants. Integrative analyses of OS-induced transcriptomes and metabolomes of natural accessions revealed that NaJAR4 is embedded in a nonlinear complex gene coexpression network orchestrating responses to OS, which we tested by silencing four hub genes in two connected coexpressed networks and examining their OS-elicited metabolic responses. Lines silenced in two hub genes (NaGLR and NaFB67) co-occurring in the NaJAR4/6 module showed responses proportional to JA-Ile accumulations; two from an adjacent module (NaERF and NaFB61) had constitutively expressed defenses with high resistance. We infer that mutations with large fitness consequences can persist in natural populations due to compensatory responses from gene networks, which allow for diversification in conserved signaling pathways and are generally consistent with predictions of an omnigene model

Oral nutrition supplements and between-meal snacks for nutrition therapy in patients with COPD identified as at nutritional risk: a randomised feasibility trial.

To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked DownloadINTRODUCTION: Intervention studies have mainly used oral nutritional supplements (ONS) for the management of patients with chronic obstructive pulmonary disease (COPD) identified as at nutritional risk. In this 12-month randomised feasibility trial, we assessed the (1) feasibility of the recruitment, retention and provision of two interventions: ONS and between-meal snacks (snacks) and (2) the potential impact of the provision of snacks and ONS on body weight and quality of life in patients with COPD. METHODS : Hospitalised patients with COPD, at nutritional risk, were randomised to ONS (n=19) or snacks (n=15) providing 600 kcal and 22 g protein a day in addition to regular daily diet. The intervention started in hospital and was continued for 12 months after discharge from the hospital. RESULTS : Study recruitment rate was n=34 (45%) and retention rate at 12 months was similar for both groups: n=13 (68%) in the ONS group and n=10 (67%) in the Snacks group. Both groups gained weight from baseline to 12 months (2.3±4.6 kg (p=0.060) in the ONS group and 4.4±6.4 kg (p=0.030) in the Snacks group). The St George's Respiratory Questionnaire total score improved from baseline to 12 months in both groups (score 3.9±11.0 (p=0.176) in the ONS group and score 8.9±14.1 (p=0.041) in the Snacks group). DISCUSSION : In patients with COPD who are at nutritional risk snacks are at least as feasible and effective as ONS, however, adequately powered trials that take account of the difficulties in recruiting this patient group are required to confirm this effect.Icelandic Research Fund of the Icelandic Centre for Research Eimskip University Fund Landspitali University Hospital Research Fund Nutricia (Icepharma