Phenotypic and Genotypic Analysis of Amino Acid Metabolism in \u3cem\u3eLactobacillus helveticus\u3c/em\u3e CNRZ 32

This study investigated genetic predictions for amino acid biosynthesis and catabolism by Lactobacillus helveticus CNRZ 32, a commercial cheese flavor adjunct that reduces bitterness and intensifies flavor notes. Conversion of amino acids into volatile and nonvolatile flavor compounds by L. helveticus and other lactic acid bacteria in cheese is thought to represent the rate-limiting step in the development of mature cheese flavor and aroma. One of the primary mechanisms for amino acid breakdown by these microbes involves the reversible action of enzymes involved in biosynthetic pathways, so our group investigated the genetics of amino acid biosynthesis in L. helveticus CNRZ 32. Most lactic acid bacteria are auxotrophic for several amino acids, and phenotypic characterization of L. helveticus CNRZ 32 has shown this bacterium requires 14 amino acids. Reconstruction of amino acid biosynthetic pathways from a draft-quality (incomplete) genome sequence for L. helveticus CNRZ 32 showed generally good agreement between gene content and phenotypic amino acid requirements. One exception involved the requirement ofCNRZ 32 for Asp (or Asn) for growth, where predictions derived from the genome sequence suggested this strain may be able to synthesize Asp from citrate. This prediction was confirmed as Asp auxotrophy in L. helveticus CNRZ 32 could be alleviated by the addition of citrate to a chemically defined medium that lacked Asp and Asn. Genome analysis also predicted that L. helveticus CNRZ 32 possessed ornithine decarboxylase activity, and would therefore catalyze the conversion of ornithine to putrescine, a volatile biogenic amine. Putrescine production in cheese would be undesirable because this compound may impart a rotting flesh flavor and can also have adverse effects on human health. Experiments to confirm ornithine decarboxylase activity in L. helveticus CNRZ 32 using a special growth medium, thin layer chromatography, high performance liquid chromatograph, or 13C nuclear magnetic resonance were unsuccessful, however, which indicated this bacterium does not contribute to putrescine production in cheese

Three & One

Faculty recital at USU.https://digitalcommons.usu.edu/music_programs/1260/thumbnail.jp

Mid-Atlantic expedition 2003/2004 : research vessel Meteor, cruise No. M60 ; 11. November 2003 – 15. April 2004

Die Meteor-Reise 60 besteht aus 5 Fahrtabschnitten im subtropischen Wirbel des Nordatlantiks von den Azoren bzw. Madeira bis zum tropischen Westatlantik. Fahrtabschnitt 1 konzentriert sich auf die Ökologie und Biogeochemie von sogenannten „Seamounts“ im östlichen Atlantik im Rahmen eines EU-Projektes; Fahrtabschnitt 2 umfasst, in Zusammenarbeit mit französischen Kollegen, detailierte seismische und geophysikalische Untersuchungen eines aktiven Segments des Mittelatlantischen Rückens; Fahrtabschnitt 3 ist eine multidisziplinäre (geologisch, biologisch und chemisch) Untersuchung über die Auswirkungen der hydrothermalen Zirkulation am Mittelatlantischen Rücken im Rahmen eines DFG-Schwerpunkt-programms; Fahrtabschnitt 4 ist einer physikalisch-ozeanographische Studie der langfristigen Variation der thermohalien Zirkulation in westlichen Becken des Atlantiks im Kontext des BMBF-CLIVAR programms gewidmet; und Fahrtabschnitt 5 schließlich, ist eine multidisziplinäre (chemisch/biologisch) Untersuchung über die CO2-Aufnahme und die biologische Pumpe in der Wassersäule des nordatlantischen Subtropenwirbels (DFG-Sonderforschungsbereich 460). Der erste Fahrtabschnitt der Reise M 60 dient der physikalischen, biogeochemischen und biologischen Probengewinnung im Rahmen des EU- Projektes OASIS (OceAnic Seamounts: an Integrated Study). OASIS ist ein interdisziplinäres Projekt mit 9 Partnern aus 5 europäischen Ländern und untersucht die funktionale Charakteristik von Seamount-Ökosystemen. Basierend auf 2 Fallstudien werden die Prozesse, die Seamount-Ökosysteme charakterisieren, und ihre Einflüsse auf den umgebenden Ozean beschrieben. Die wissenschaftlichen Ergebnisse, zusammengeführt in einem konzeptionellen Ökosystemmodell, werden Ausgangsbasis für die Entwicklung von generellen und ortspezifischen Managementplänen sein. Das primäre Ziel von OASIS, eine holistische Erfassung von Seamount-Ökosystemen, wird durch die Integration folgender Sachgebiete erreicht: a) Identifikation und Beschreibung der physikalischen Einflußkräfte auf Seamount-Ökosysteme b) Erfassung der Herkunft, Qualität und Dynamik partikulärer organischer Substanz in der Wassersäule und an der Sedimentoberfläche c) Beschreibung von Aspekten der Biodiversität und Ökologie von Seamount-Lebensgemeinschaften und Erfassung ihrer Dynamik und Produktionsmechanismen d) Modellierung der trophischen Ökologie von Seamount-Ökosystemen e) Anwendung der wissenschaftlichen Ergebnisse auf den Naturschutz. Ein weiteres Ziel der Fahrt ist die Erprobung eines neu entwickelten Drucklabors zur Untersuchung von Tiefseeorganismen unter in situ-Druckbdingenungen von bis zu 500 bar.The Meteor 60 expedition is comprised of 5 legs covering the sub-tropical gyre of the North Atlantic from the Azores and Madeira to the tropical western Atlantic. Leg 1 focuses on the ecology and biogeochemistry of seamounts in the eastern Atlantic in the context of an EU program; Leg 2 comprises a detailed seismic and geophysical investigation of a propagating ridge segment at the Mid-Atlantic Ridge in cooperation with French scientists; Leg 3 is a multidisciplinary (geological, biological and chemical) investigation of the effects of hydrothermal circulation at the Mid-Atlantic ridge in support of a DFG Special Priority Program; Leg 4 is a physical oceanographic study of long-term variation of the thermohaline circulation in the western basin of the Atlantic in the context of the BMBF-CLIVAR program; and Leg 5 is a multidisciplinary (chemical/biological) investigation of CO2 uptake and the biological pump in the water column of the North Atlantic sub-tropical gyre (DFG Special Research Area 460). The first leg of cruise M 60 aims at physical, biogeochemical and biological sampling in the framework of the EU project OASIS (OceAnic Seamounts: an Integrated Study). OASIS is an interdisciplinary project and comprises 9 partners from 5 European countries. The project studies the functional characteristics of seamount ecosystems. Based on two case studies, OASIS will yield an advanced mechanistic understanding of the processes characterizing seamount ecosystems, and their influence on the surrounding ocean. The scientific knowledge gained, condensed in a conceptual ecosystem model, will be applied to outline a model management plan as well as site-specific management plans for the seamounts investigated The primary goal of OASIS, to provide a holistic, integrated assessment of seamount ecology, will be achieved by addressing the following main objectives: a) To identify and describe the physical forcing mechanisms effecting seamount systems. b) To assess the origin, quality and dynamics of particulate organic material within the water column and surface sediment at seamounts. c) To describe aspects of the biodiversity and the ecology of seamount biota, to assess their dynamics and the maintenance of their production. d) Modelling the trophic ecology of seamount ecosystems. e) Application of scientific knowledge to practical conservation. A further goal of the cruise will be to test a newly developed hyperbaric laboratory which is designed to study deep-sea organisms under in situ pressure

Do low-calorie sweetened beverages help to control food cravings? Two experimental studies

Low-calorie sweetened (LCS) beverages may help consumers to satisfy hedonic food cravings without violating dieting goals, however this remains unexplored. The present research investigated the effect of priming hedonic eating motivations on ad libitum energy intake in frequent and non-consumers of LCS beverages. It was hypothesised that energy intake would be greater after the hedonic eating prime relative to a control prime in non-consumers, but that frequent LCS beverage consumers would be protected from this effect. In Study 1 (N = 120), frequent and non-consumers were exposed to either chocolate or neutral cues (craving vs. control condition) and then completed a beverage-related visual probe task with concurrent eye-tracking. Ad libitum energy intake from sweet and savoury snacks and beverages (including LCS) was then assessed. Study 2 followed a similar protocol, but included only frequent consumers (N = 172) and manipulated the availability of LCS beverages in the ad libitum eating context (available vs. unavailable). Measures of guilt and perceived behavioural control were also included. In Study 1, as hypothesised, non-consumers showed greater energy intake in the craving condition relative to the control condition, but frequent consumers had similar energy intake in both conditions. Frequent consumers (but not non-consumers) also demonstrated an attentional bias for LCS beverage stimuli compared to both sugar and water stimuli. In contrast, in Study 2 frequent consumers showed greater energy intake in the craving condition relative to the control condition; however, overall energy intake was significantly greater when LCS beverages were unavailable compared to when they were available. Ratings of guilt were higher and perceived control was lower in the LCS-unavailable condition relative to the LCS-available condition. Conclusions: LCS beverages did not consistently protect consumers from craving-induced increases in energy intake. However, frequent consumers consumed fewer calories overall when LCS beverages were available (relative to unavailable), as well as perceiving more control over their food intake and feeling less guilty

Understanding decision-making in prosthetic rehabilitation by prosthetists and people with lower limb amputation: a qualitative study.

PURPOSE: Little has been published about the process of decision-making between prosthetists and people with lower limb amputation (LLA). The purpose of this study is to identify decisions and factors influencing decision-making in prosthetic rehabilitation from the perspectives of prosthetists and prosthesis users, to identify barriers and opportunities for shared decision-making (SDM). METHODS: Qualitative semi-structured individual interviews were conducted with 13 prosthetists and 14 prosthesis users from three clinics in three states of the Rocky Mountain and Southwest regions of the United States. Transcripts were analyzed using thematic analysis. RESULTS: Four main themes were identified: perceived decision points, importance of relationship, balancing competing priorities, and experience. Contrasts between perceptions of prosthetists and prosthesis users were related to prosthesis design decisions, and the purpose of communication (e.g., goals for a prosthesis vs. goals informing prosthesis design). Both prosthetists and prosthesis users described balancing priorities that contribute to prosthetic rehabilitation decisions, and the role of experience for informing realistic expectations and preferences necessary for participating in decision-making. CONCLUSION: Opportunities for improving SDM between prosthetists and prosthesis users include (1) clarifying key rehabilitation decisions, (2) identifying the purpose of initial communications, (3) support for balancing priorities, and (4) utilizing experience to achieve informed preferences.IMPLICATIONS FOR REHABILITATIONMany people with lower limb amputation experience poor physical function and psychosocial outcomes, which may be further compounded by under informed prosthesis-user expectations for function with a prosthesis.Shared decision-making offers an opportunity for improving realistic prosthesis-user expectations, reducing healthcare costs, and improving prosthesis-user satisfaction and adherence to care plans.Opportunities for improving shared decision-making between prosthetists and prosthesis-users include (1) clarifying key rehabilitation decisions, (2) identifying the purpose of initial communications, (3) support for balancing priorities, and (4) utilizing experience to achieve informed preferences

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Morphologic heterogeneity within an individual tumor is well-recognized by histopathologists in surgical practice. While this often takes the form of areas of distinct differentiation into recognized histological subtypes, or different pathological grade, often there are more subtle differences in phenotype which defy accurate classification (Figure 1). Ultimately, since morphology is dictated by the underlying molecular phenotype, areas with visible differences are likely to be accompanied by differences in the expression of proteins which orchestrate cellular function and behavior, and therefore, appearance. The significance of visible and invisible (molecular) heterogeneity for prognosis is unknown, but recent evidence suggests that, at least at the genetic level, heterogeneity exists in the primary tumor1,2, and some of these sub-clones give rise to metastatic (and therefore lethal) disease

A Class of Eccentric Binaries with Dynamic Tidal Distortions Discovered with Kepler

We have discovered a class of eccentric binary systems within the Kepler data archive that have dynamic tidal distortions and tidally-induced pulsations. Each has a uniquely shaped light curve that is characterized by periodic brightening or variability at time scales of 4-20 days, frequently accompanied by shorter period oscillations. We can explain the dominant features of the entire class with orbitally-varying tidal forces that occur in close, eccentric binary systems. The large variety of light curve shapes arises from viewing systems at different angles. This hypothesis is supported by spectroscopic radial velocity measurements for five systems, each showing evidence of being in an eccentric binary system. Prior to the discovery of these 17 new systems, only four stars, where KOI-54 is the best example, were known to have evidence of these dynamic tides and tidally-induced oscillations. We perform preliminary fits to the light curves and radial velocity data, present the overall properties of this class and discuss the work required to accurately model these systems.Comment: 13 pages, submitted to Ap

Associations between hedonic hunger and BMI during a two-year behavioural weight loss trial.

ObjectiveProspective studies on relationships between hedonic hunger and BMI (Body Mass Index) during weight management are lacking. This study examined if hedonic hunger reduced during a behavioural weight management programme, and if hedonic hunger predicted future BMI.MethodsParticipants were 594 community-dwelling, UK-based adults(396 female; age 56.43 years, s.d. = 12.50, range 20-83 years); 490 participants (82.5%) had obesity. Participants were randomised to a 12- or 52-week behavioural weight management intervention (WW12 or WW52, respectively) or a brief self-help intervention (BI). Relationships between hedonic hunger and BMI over 24 months (baseline, 3, 12, 24 months) were analysed using an autoregressive cross-lagged model.ResultsHedonic hunger scores decreased from 2.71 (s.d. = .91) at baseline to 2.41 (s.d. = .88) at 3 months (p .05). Baseline hedonic hunger scores predicted 3-month scores (B = .76, SE = .03, p .05). BMI at 12 months was lower in WW52 30.87kg/m2, s.d. = 5.02) than WW12 (32.12 kg/m2, s.d. = 5.58, p = .02, CI .16 to 2.34) and BI (32.74 kg/m2, s.d. = 4.15, p = .01, CI .30 to 3.45). BMI was not affected by intervention at any other time point (p's>.05).ConclusionHedonic hunger reduced during weight management irrespective of intervention. Early reductions in hedonic hunger appear to be associated with lower BMI in the medium-term. Identifying ways to reduce hedonic hunger during weight loss could aid weight management for some people