197 research outputs found
A taxonomic and phylogenetic revision of Penicillium section Aspergilloides
AbstractSpecies belonging to Penicillium section Aspergilloides have a world-wide distribution with P. glabrum, P. spinulosum and P. thomii the most well-known species of this section. These species occur commonly and can be isolated from many substrates including soil, food, bark and indoor environments. The taxonomy of these species has been investigated several times using various techniques, but species delimitation remains difficult. In the present study, 349 strains belonging to section Aspergilloides were subjected to multilocus molecular phylogenetic analyses using partial β-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) sequences. Section Aspergilloides is subdivided into 12 clades and 51 species. Twenty-five species are described here as new and P. yezoense, a species originally described without a Latin diagnosis, is validated. Species belonging to section Aspergilloides are phenotypically similar and most have monoverticillate conidiophores and grow moderately or quickly on agar media. The most important characters to distinguish these species were colony sizes on agar media, growth at 30 °C, ornamentation and shape of conidia, sclerotium production and stipe roughness
Magnetic states of granular layered CoFe-Al\u3csub\u3e2\u3c/sub\u3eO\u3csub\u3e3\u3c/sub\u3e
The granular layered magnetic system Co80Fe20(t)/Al2 O3 (3 nm), where the Co80Fe20 layers of nominal thickness t form separate, almost spherical magnetic granules of typical diameter 2-3 nm between the Al2O3 spacers, was studied. We discuss measurements of the dc and ac magnetic susceptibility χ for 1 n
Endothelial loss of Fzd5 stimulates PKC/Ets1-mediated transcription of Angpt2 and Flt1
Aims: Formation of a functional vascular system is essential and its formation is a highly regulated process initiated during embryogenesis, which continues to play important roles throughout life in both health and disease. In previous studies, Fzd5 was shown to be critically involved in this process and here we investigated the molecular mechanism by which endothelial loss of this receptor attenuates angiogenesis. Methods and results: Using short interference RNA-mediated loss-of-function assays, the function and mechanism of signaling via Fzd5 was studied in human endothelial cells (ECs). Our findings indicate that Fzd5 signaling promotes neovessel formation in vitro in a collagen matrix-based 3D co-culture of primary vascular cells. Silencing of Fzd5 reduced EC proliferation, as a result of G0/G1 cell cycle arrest, and decreased cell migration. Furthermore, Fzd5 knockdown resulted in enhanced expression of the factors Angpt2 and Flt1, which are mainly known for their destabilizing effects on the vasculature. In Fzd5-silenced ECs, Angpt2 and Flt1 upregulation was induced by enhanced PKC signaling, without the involvement of canonical Wnt signaling, non-canonical Wnt/Ca2+-mediated activation of NFAT, and non-canonical Wnt/PCP-mediated activation of JNK. We demonstrated that PKC-induced transcription of Angpt2 and Flt1 involved the transcription factor Ets1. Conclusions: The current study demonstrates a pro-angiogenic role of Fzd5, which was shown to be involved in endothelial tubule formation, cell cycle progression and migration, and partly does so by repression of PKC/Ets1-mediated transcription of Flt1 and Angpt2
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Experimental and Numerical Examination of the Thermal Transmittance of High Performance Window Frames
While window frames typically represent 20-30percent of the overall window area, their impact on the total window heat transfer rates may be much larger. This effect is even greater in low-conductance (highly insulating) windows which incorporate very low conductance glazings. Developing low-conductance window frames requires accurate simulation tools for product research and development. The Passivhaus Institute in Germany states that windows (glazing and frames, combined) should have U-values not exceeding 0.80 W/(m??K). This has created a niche market for highly insulating frames, with frame U-values typically around 0.7-1.0 W/(m2 cdot K). The U-values reported are often based on numerical simulations according to international simulation standards. It is prudent to check the accuracy of these calculation standards, especially for high performance products before more manufacturers begin to use them to improve other product offerings. In this paper the thermal transmittance of five highly insulating window frames (three wooden frames, one aluminum frame and one PVC frame), found from numerical simulations and experiments, are compared. Hot box calorimeter results are compared with numerical simulations according to ISO 10077-2 and ISO 15099. In addition CFD simulations have been carried out, in order to use the most accurate tool available to investigate the convection and radiation effects inside the frame cavities. Our results show that available tools commonly used to evaluate window performance, based on ISO standards, give good overall agreement, but specific areas need improvement
New Insights into White-Light Flare Emission from Radiative-Hydrodynamic Modeling of a Chromospheric Condensation
(abridged) The heating mechanism at high densities during M dwarf flares is
poorly understood. Spectra of M dwarf flares in the optical and
near-ultraviolet wavelength regimes have revealed three continuum components
during the impulsive phase: 1) an energetically dominant blackbody component
with a color temperature of T 10,000 K in the blue-optical, 2) a smaller
amount of Balmer continuum emission in the near-ultraviolet at lambda 3646
Angstroms and 3) an apparent pseudo-continuum of blended high-order Balmer
lines. These properties are not reproduced by models that employ a typical
"solar-type" flare heating level in nonthermal electrons, and therefore our
understanding of these spectra is limited to a phenomenological interpretation.
We present a new 1D radiative-hydrodynamic model of an M dwarf flare from
precipitating nonthermal electrons with a large energy flux of erg
cm s. The simulation produces bright continuum emission from a
dense, hot chromospheric condensation. For the first time, the observed color
temperature and Balmer jump ratio are produced self-consistently in a
radiative-hydrodynamic flare model. We find that a T 10,000 K
blackbody-like continuum component and a small Balmer jump ratio result from
optically thick Balmer and Paschen recombination radiation, and thus the
properties of the flux spectrum are caused by blue light escaping over a larger
physical depth range compared to red and near-ultraviolet light. To model the
near-ultraviolet pseudo-continuum previously attributed to overlapping Balmer
lines, we include the extra Balmer continuum opacity from Landau-Zener
transitions that result from merged, high order energy levels of hydrogen in a
dense, partially ionized atmosphere. This reveals a new diagnostic of ambient
charge density in the densest regions of the atmosphere that are heated during
dMe and solar flares.Comment: 50 pages, 2 tables, 13 figures. Accepted for publication in the Solar
Physics Topical Issue, "Solar and Stellar Flares". Version 2 (June 22, 2015):
updated to include comments by Guest Editor. The final publication is
available at Springer via http://dx.doi.org/10.1007/s11207-015-0708-
Investigation of the role of Li resonances in the halo structure of Li through the Li(p, d)Li transfer reaction
International audienceThe first measurement of the one-neutron transfer reaction 11Li(p,d)10Li performed using the IRIS facility at TRIUMF with a 5.7AMeV11Li beam interacting with a solid H2 target is reported. The 10Li residue was populated strongly as a resonance peak with energy Er=0.62 ±0.04MeV having a total width = 0.33 ±0.07MeV. The angular distribution of this resonance is characterized by neutron occupying the 1p1/2orbital. A DWBA analysis yields a spectroscopic factor of 0.67 ±0.12for p1/2 removal strength from the ground state of 11Li to the region of the peak
Influence of Bleeding Risk on Outcomes of Radial and Femoral Access for Percutaneous Coronary Intervention: An Analysis From the GLOBAL LEADERS Trial
Background: Radial artery access has been shown to reduce mortality and bleeding events, especially in patients with acute coronary syndromes. Despite this, interventional cardiologists experienced in femoral artery access still prefer that route for percutaneous coronary intervention. Little is known regarding the merits of each vascular access in patients stratified by their risk of bleeding. Methods: Patients from the Global Leaders trial were dichotomized into low or high risk of bleeding by the median of the PRECISE-DAPT score. Clinical outcomes were compared at 30 days. Results: In the overall population, there were no statistical differences between radial and femoral access in the rate of the primary end point, a composite of all-cause mortality, or new Q-wave myocardial infarction (MI) (hazard ratio [HR] 0.70, 95% confidence interval [CI] 0.42-1.15). Radial access was associated with a significantly lower rate of the secondary safety end point, Bleeding Academic Research Consortium (BARC) 3 or 5 bleeding (HR 0.55, 95% CI 0.36-0.84). Compared by bleeding risk strata, in the high bleeding score population, the primary (HR 0.47, 95% CI 0.26-0.85; P = 0.012; Pinteraction = 0.019) and secondary safety (HR 0.57, 95% CI 0.35-0.95; P = 0.030; Pinteraction = 0.631) end points favoured radial access. In the low bleeding score population, however, the differences in the primary and secondary safety end points between radial and femoral artery access were no longer statistically significant. Conclusions: Our findings suggest that the outcomes of mortality or new Q-wave MI and BARC 3 or 5 bleeding favour radial access in patients with a high, but not those with a low, risk of bleeding. Because thisContexte : Il a et e d emontr e que l ’accès par l’artère radiale reduit la
mortalite et les h emorragies, en particulier chez les patients
presentant un syndrome coronarien aigu. Malgr e cela, les cardiologues
interventionnels qui ont acquis de l’experience en matière d ’accès par
l’artère femorale pr efèrent encore utiliser cette voie lorsqu ’ils doivent
pratiquer une intervention coronarienne percutanee. On connaît mal l’interêt de chacune de ces techniques d ’accès vasculaire au regard du
risque d’hemorragie.
Methodologie : Les patients de l’essai GLOBAL LEADERS ont et e
repartis en deux groupes, selon qu ’ils presentaient un risque
d’hemorragie faible ou elev e d ’après le score PRECISE-DAPT median,
puis les resultats cliniques ont et e compar es à 30 jours.
Resultats : Dans l’ensemble de la population, aucune difference sta-
tistiquement significative n’a et e observ ee entre l ’accès radial et
l’accès femoral quant au critère d ’evaluation principal, compos e de la
mortalite toutes causes confondues et d ’un nouvel infarctus du myocarde (IM) avec onde Q (rapport des risques instantanes [RRI] de 0,70;
intervalle de confiance [IC] à 95 % : 0,42-1,15). L’accès radial a et e
associe à un taux signi ficativement plus faible de survenue du critère
secondaire d’evaluation de l ’innocuite, c ’est-à-dire une hemorragie de
type 3 ou 5 selon la classification du BARC (Bleeding Academic
Research Consortium) (RRI de 0,55; IC à 95 % : 0,36-0,84). Lorsqu’on
compare les sujets en fonction du risque d’hemorragie, les critères
d’evaluation de l ’innocuite principal (RRI de 0,47; IC à 95 % : 0,26-
0,85; p ¼ 0,012; pinteraction ¼ 0,019) et secondaire (RRI de 0,57; IC à
95 % : 0,35-0,95; p ¼ 0,030; pinteraction ¼ 0,631) sont favorables à
l’accès radial au sein de la population presentant un risque d ’hemor-
ragie elev e. Dans la population pr esentant un risque d ’hemorragie
faible, les differences entre l ’accès radial et l’accès femoral quant aux
critères d’evaluation de l ’innocuite principal et secondaire ne sont
toutefois plus statistiquement significatives.
Conclusions : Selon ces observations, les resultats concernant la
mortalite ou la survenue d ’un nouvel IM avec onde Q et le risque
d’hemorragie de type 3 ou 5 selon la classi fication du BARC indiquent
que l’accès radial serait à privilegier lorsque le risque d ’hemorragie est
elev e, mais pas lorsqu ’il est faible. Comme il ne s’agissait pas d’une
analyse principale, il convient de considerer ces observations comme
etant g en eratrices d ’hypothèses
Discovery and Fine-Mapping of Glycaemic and Obesity-Related Trait Loci Using High-Density Imputation
Reference panels from the 1000 Genomes (1000G) Project Consortium provide near complete coverage of common and low-frequency genetic variation with minor allele frequency ≥0.5% across European ancestry populations. Within the European Network for Genetic and Genomic Epidemiology (ENGAGE) Consortium, we have undertaken the fi
Comparison of Physical-chemical and Mechanical Properties of Chlorapatite and Hydroxyapatite Plasma Sprayed Coatings
Chlorapatite can be considered a potential biomaterial for orthopaedic applications. Its use as plasma-sprayed coating could be of interest considering its thermal properties and particularly its ability to melt without decomposition unlike hydroxyapatite. Chlorapatite (ClA) was synthesized by a high-temperature ion exchange reaction starting from commercial stoichiometric hydroxyapatites (HA). The ClA powder showed similar characteristics as the original industrial HA powder, and was obtained in the monoclinic form. The HA and ClA powders were plasma-sprayed using a low-energy plasma spraying system with identical processing parameters. The coatings were characterized by physical-chemical methods, i.e. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy, including distribution mapping of the main phases detected such as amorphous calcium phosphate (ACP), oxyapatite (OA), and HA or ClA. The unexpected formation of oxyapatite in ClA coatings was assigned to a side reaction with contaminating oxygenated species (O2, H2O). ClA coatings exhibited characteristics different from HA, showing a lower content of oxyapatite and amorphous phase. Although their adhesion strength was found to be lower than that of HA coatings, their application could be an interesting alternative, offering, in particular, a larger range of spraying conditions without formation of massive impurities.This study was carried out under a MNT ERA-Net Project named NANOMED. The authors gratefully thank the Midi-Pyrénées region (MNT ERA Net Midi-Pyrénées Région, NANOMED2 project) and the Institute National Polytechnique de Toulouse (BQR INPT 2011, BIOREVE project) for supporting this research work, especially the financial support for research carried out in the CIRIMAT and the LGP laboratories (France), and the Basque government and Tratamientos Superficiales Iontech, S.A. for their financial and technical support under the IG-2007/0000381 grant for the development of the LEPS device and deposition of the coatings carried out in Inasmet-Tecnalia.
The French industrial collaborators (TEKNIMED SA and 2PS SA) were financed by the OSEO programs
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