Edible bamboo resources of Manipur: consumption pattern of young shoots, processing techniques and their commercial status in the local market

Manipur constitutes one of the major hubs of bamboo resource in the country. Apart from its household and industrial uses, bamboo is also revered in the state for its dietary usefulness through consumption of its tender shoots. To quantify the magnitude of usage patterns of bamboo shoots for food in the state, a detailed survey was undertaken covering all the 16 districts and 21 main markets of Manipur. Of the 53 reported species, 15 species belonging to genera Bambusa, Cephalostachyum, Chimonobambusa, Dendrocalamus and Melocanna were recorded as major edible bamboo species consumed as food in different areas of Manipur. Dendrocalamus latiflorus emerged as the most popular edible bamboo species in the state, being consumed in all the 16 districts. Along with fresh shoots, boiled, fermented, pickled and dried forms are also consumed. Different traditional processing and preservation methods of bamboo shoots are also practised in the state of which fermentation is the most popular one. In market places, fresh shoots are sold during the monsoon season whereas fermented shoots are available throughout the year while the sales of other bamboo shoot products in local markets are in a very nascent state

Edible bamboo resources of Manipur: consumption pattern of young shoots, processing techniques and their commercial status in the local market

73-82Manipur constitutes one of the major hubs of bamboo resource in the country. Apart from its household and industrial uses, bamboo is also revered in the state for its dietary usefulness through consumption of its tender shoots. To quantify the magnitude of usage patterns of bamboo shoots for food in the state, a detailed survey was undertaken covering all the 16 districts and 21 main markets of Manipur. Of the 53 reported species, 15 species belonging to genera Bambusa, Cephalostachyum, Chimonobambusa, Dendrocalamus and Melocanna were recorded as major edible bamboo species consumed as food in different areas of Manipur. Dendrocalamus latiflorus emerged as the most popular edible bamboo species in the state, being consumed in all the 16 districts. Along with fresh shoots, boiled, fermented, pickled and dried forms are also consumed. Different traditional processing and preservation methods of bamboo shoots are also practised in the state of which fermentation is the most popular one. In market places, fresh shoots are sold during the monsoon season whereas fermented shoots are available throughout the year while the sales of other bamboo shoot products in local markets are in a very nascent state

Effects of flanking sequences and cellular context on subcellular behavior and pathology of mutant HTT

Huntington’s disease (HD) is caused by an expansion of a poly glutamine (polyQ) stretch in the huntingtin protein (HTT) that is necessary to cause pathology and formation of HTT aggregates. Here we ask whether expanded polyQ is sufficient to cause pathology and aggregate formation. By addressing the sufficiency question, one can identify cellular processes and structural parameters that influence HD pathology and HTT subcellular behavior (i.e. aggregation state and subcellular location). Using Drosophila, we compare the effects of expressing mutant full-length human HTT (fl-mHTT) to the effects of mutant human HTTexon1 and to two commonly used synthetic fragments, HTT171 and shortstop (HTT118). Expanded polyQ alone is not sufficient to cause inclusion formation since full-length HTT and HTTex1 with expanded polyQ are both toxic although full-length HTT remains diffuse while HTTex1 forms inclusions. Further, inclusions are not sufficient to cause pathology since HTT171-120Q forms inclusions but is benign and co-expression of HTT171-120Q with non-aggregating pathogenic fl-mHTT recruits fl-mHTT to aggregates and rescues its pathogenicity. Additionally, the influence of sequences outside the expanded polyQ domain is revealed by finding that small modifications to the HTT118 or HTT171 fragments can dramatically alter their subcellular behavior and pathogenicity. Finally, mutant HTT subcellular behavior is strongly modified by different cell and tissue environments (e.g. fl-mHTT appears as diffuse nuclear in one tissue and diffuse cytoplasmic in another but toxic in both). These observations underscore the importance of cellular and structural context for the interpretation and comparison of experiments using different fragments and tissues to report the effects of expanded polyQ

AMINO ACID PROFILING IN EDIBLE SHOOTS OF SOME BAMBOO SPECIES BY USING RP-UHPLC AND THEIR THERAPEUTICAL ASPECTS

Objective: This study was conducted to assess the amino acid profiles of edible shoots of five species of bamboos growing in various geographical regions of India. Methods: Qualitative and quantitative estimation of free amino acids was done by Reverse Phase-Ultra High Performance Liquid Chromatography (RP-UHPLC). Separation of amino acids was carried out using Zorbax eclipse plus C18 Reverse Phase (1.8 μm), 50 mm × 2.1 mm column on quaternary UHPLC system using modified gradient elution. Derivatization was done by using auto-sampler Agilent’s 1290 infinity. Data assortment and processing were accomplished by using Open Lab Chromatography Data System EZchrome edition, version A.04.06 (Agilent technologies, USA). Results: In the study, 19 free amino acids were identified, eight essential, five conditionally essential, and six non-essential amino acids (EAA). The principal amino acids were tyrosine (29.41–74.64 μg/mg d.w.) and asparagine (12.81–111.04 μg/mg d.w.). The amount of EAAs ranged from 0.46 to 21.11 μg/mg d.w. For the 1st time, taurine and ornithine are reported in bamboo shoots. The analysis exhibited significant variation (p<0.05) in free amino acids content among the five studied species. Of the five species, Phyllostachys mannii was best compared to the other species in amino acid content. Conclusion: Results showed superior amino acid composition of bamboo shoots that can be utilized to improve nutritional value of functional foods. Amino acids have various health and therapeutic properties can contribute to the basis for further expansion of these bamboos and imminent potential in applications of food and pharmaceuticals

Dengue Serotypes In Manipur – Findings From A Retrospective Analytical Study In A Tertiary Care Hospital

Objectives: As a part of on-going research programme on vector-borne viral diseases especially on Dengue, a retrospective analytical study on the occurrence and distribution of Dengue virus serotype(s) in the state of Manipur – a small state situated in the north eastern region of the Indian subcontinent was carried out at Viral Research & Diagnostic Laboratory (VRDL), Department of Microbiology, Regional Institute of Medical Sciences (RIMS), Imphal which is a tertiary care hospital. Materials & Methods: A total of 914 blood samples from clinically dengue suspected patients were screened for the presence of Dengue infection by adopting ELISA (IgM) technique during the period from 01/06/2022 to 02/12/2022. Further, anti-Dengue IgM antibody positive samples having high Optical Density (OD) value(s) were selected and subjected to RT PCR to determine the serotype(s) of Dengue virus. Results: Of the 914 blood samples examined for the presence of Dengue infection, 111 (12.14%) were found positive for anti-Dengue IgM antibody indicating acute infection of Dengue virus. Of the positive patients, there were 56 (50.45%) males and 55 (49.54%) females. The sex difference was found to be statistically insignificant (।z।CV = 0.016; z ˂ 1.96; P ˂ 0.05). Predominant clinical features observed among the Dengue confirmed patients included – fever (74%), headache (19%), arthralgia / joint pain (9%), myalgia (6%) and vomiting (6%) respectively. Haemorrhagic manifestation was observed in only one (01) patient. Only three (03) Dengue positive patients had the history of travelling outside the state. The study revealed that while circulation of three (03) Dengue virus serotypes, namely DENV – 1, DENV – 2 & DENV – 3 were observed in the Tengnoupal district, the circulation of two Dengue virus serotypes i.e., DENV – 1 & DENV – 2 were evident in Imphal West & Bishnupur districts respectively. The present study also reveals the occurrence of Dengue virus serotype – 1 (DENV - 1) in Churachandpur district. Conclusion: The present study reveals the circulation / distribution of three Dengue virus serotypes namely, DENV – 1, DENV – 2 and DENV – 3 among the studied samples. Findings from this pilot study stresses the need for continuing more in-depth studies among the population of the state towards identifying serotypes endemic/prevalent in the different districts of the state. Keywords: Dengue virus, Dengue IgM ELISA, Dengue serotype (RT PCR

Diversifying European agricultural systems by intercropping grain legumes and cereals

Cropping system diversification is a key factor in developing more sustainable cropping and food systems. The agroecological practice of intercropping, meaning the simultaneous cultivation of two or more species in the same field, has recently gained renewed interest as a means of ecological intensification in European agricultural research. We discuss some recent research developments regarding 1) intercropping for ecological intensification in agroecological and conventional cropping systems, 2) studies on nitrogen resource use by cereal-grain legume intercropping cultivation, 3) the role of intercropping in the management of biotic stressors, especially weeds, and 4) intercropping as a means of creating cropping systems that are more resilient to the abiotic and biotic stress associated with climate change. Finally, we propose methods for the greater adoption of intercropping in European agriculture by unlocking farming systems from upstream and downstream barriers, with the aim of developing more sustainable agricultural and food systems

Melanosis and quality attributes of chill stored farm raised whiteleg shrimp (Litopenaeus vannamei)

Loss of market value of shrimp is mainly due to the formation of black spot called melanosis. A study was conducted for 14 days to determine the extent of melanosis and quality changes during that period of freshly har-vested whiteleg shrimp (Litopenaeus vannamei) under chilled storage (2?). Among quality parameters, total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substances (TBAR-S), were varied from 13.17 mg % to 44.50 mg % and 0.04to 2.57 mg malondehaldehyde/kg of fat respectively whereas melanosis score and metric chroma (C) exhibited significant increases during chilled storage (P<0.05). There was a slight increase in moisture, crude fat and pH from 73.96 % to 74.57 %, 1.05 % to 1.14 % and 6.52 to 7.60 respectively at 14th day of storage. Loss of protein from 22.51 % to 21.28 % may be due to decrease in available amino acids during chilled storage and total plate count (TPC) showed gradual increase of bacterial load up to 1.73*107 log CFU/g at the end of chilled storage. The sensory analysis by panellists indicated, the acceptability of white leg shrimp was up to 6 days in chilled condition and formation of black spot is one of the major parameter for rejection by the panellists