Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Media intrusion or development journalism? Framing the 2006 Singapore General Election.

This study examined the dominant type of frame in Singapore press coverage of the 2006 Singapore General Election by pitting two theories against each other. While media intrusion theory states that media adopt game frames as a result of following journalistic values that favor the competitive aspects of campaigns, the development journalism model predicts more issue frames since it emphasizes issues and policies. Election news articles that appeared in The Straits Times, the dominant English newspaper in Singapore, over a 16-day period were content analyzed. Game-framed coverage predominated, lending support to media intrusion theory rather than the development model. Objectivity of the press was also examined, revealing a bias toward the ruling People’s Action Party to some extent. The findings indicate that Singapore’s dominant newspaper follows international journalistic conventions in election coverage, emphasizing strategic contests between candidates and parties instead of reporting on substantive issues that are ultimately more important to voters. However, favorable coverage toward the government also points to presence of some aspects of the development model. By following the agenda of the government and casting it in a favorable light, Singapore’s dominant newspaper exhibited characteristics of development journalism.Bachelor of Communication Studie

Advances of metaheuristic algorithms in training neural networks for industrial applications

In recent decades, researches on optimizing the parameter of the artificial neural network (ANN) model has attracted significant attention from researchers. Hybridization of superior algorithms helps improving optimization performance and capable of solving complex applications. As a traditional gradient-based learning algorithm, ANN suffers from a slow learning rate and is easily trapped in local minima when training techniques such as gradient descent (GD) and back-propagation (BP) algorithm are used. The characteristics of randomization and selection of the best or near-optimal solution of metaheuristic algorithm provide an effective and robust solution; therefore, it has always been used in training of ANN to improve and overcome the above problems. New metaheuristic algorithms are proposed every year. Therefore, the review of its latest developments is essential. This article attempts to summarize the metaheuristic algorithms which have been proposed from the year 1975 to 2020 from various journals, conferences, technical papers, and books. The comparison of the popularity of the metaheuristic algorithm is presented in two time frames, such as algorithms proposed in the recent 20 years and those proposed earlier. Then, some of the popular metaheuristic algorithms and their working principle are reviewed. This article further categorizes the latest metaheuristic search algorithm in the literature to indicate their efficiency in training ANN for various industry applications. More and more researchers tend to develop new hybrid optimization tools by combining two or more metaheuristic algorithms to optimize the training parameters of ANN. Generally, the algorithm’s optimal performance must be able to achieve a fine balance of their exploration and exploitation characteristics. Hence, this article tries to compare and summarize the properties of various metaheuristic algorithms in terms of their convergence rate and the ability to avoid the local minima. This information is useful for researchers working on algorithm hybridization by providing a good understanding of the convergence rate and the ability to find a global optimum

Complex Impedance and Modulus Analysis on Porous and Non-Porous Scaffold Composites Due to Effect of Hydroxyapatite/Starch Proportion

This study aims to investigate the electric responses (complex modulus and complex impedance analysis) of hydroxyapatite/starch bone scaffold as a function of hydroxyapatite/starch proportion and the microstructural features. Hence, the non-porous and porous hydroxyapatite/starch composites were fabricated with various hydroxyapatite/starch proportions (70/30, 60/40, 50/50, 40/60, 30/70, 20/80, and 10/90 wt/wt%). Microstructural analysis of the porous hydroxyapatite/starch composites was carried out by using scanning electron microscopy. It shows that the formation of hierarchical porous microstructures with high porosity is more significant at a high starch proportion. The complex modulus and complex impedance analysis were conducted to investigate the electrical conduction mechanism of the hydroxyapatite/starch composites via dielectric spectroscopy within a frequency range from 5 MHz to 12 GHz. The electrical responses of the hydroxyapatite/starch composites are highly dependent on the frequency, material proportion, and microstructures. High starch proportion and highly porous hierarchical microstructures enhance the electrical responses of the hydroxyapatite/starch composite. The material proportion and microstructure features of the hydroxyapatite/starch composites can be indirectly reflected by the simulated electrical parameters of the equivalent electrical circuit models

The surgical safety checklist and patient outcomes after surgery: a prospective observational cohort study, systematic review and meta-analysis

© 2017 British Journal of Anaesthesia Background: The surgical safety checklist is widely used to improve the quality of perioperative care. However, clinicians continue to debate the clinical effectiveness of this tool. Methods: Prospective analysis of data from the International Surgical Outcomes Study (ISOS), an international observational study of elective in-patient surgery, accompanied by a systematic review and meta-analysis of published literature. The exposure was surgical safety checklist use. The primary outcome was in-hospital mortality and the secondary outcome was postoperative complications. In the ISOS cohort, a multivariable multi-level generalized linear model was used to test associations. To further contextualise these findings, we included the results from the ISOS cohort in a meta-analysis. Results are reported as odds ratios (OR) with 95% confidence intervals. Results: We included 44 814 patients from 497 hospitals in 27 countries in the ISOS analysis. There were 40 245 (89.8%) patients exposed to the checklist, whilst 7508 (16.8%) sustained ≥1 postoperative complications and 207 (0.5%) died before hospital discharge. Checklist exposure was associated with reduced mortality [odds ratio (OR) 0.49 (0.32–0.77); P\u3c0.01], but no difference in complication rates [OR 1.02 (0.88–1.19); P=0.75]. In a systematic review, we screened 3732 records and identified 11 eligible studies of 453 292 patients including the ISOS cohort. Checklist exposure was associated with both reduced postoperative mortality [OR 0.75 (0.62–0.92); P\u3c0.01; I2=87%] and reduced complication rates [OR 0.73 (0.61–0.88); P\u3c0.01; I2=89%). Conclusions: Patients exposed to a surgical safety checklist experience better postoperative outcomes, but this could simply reflect wider quality of care in hospitals where checklist use is routine

Prospective observational cohort study on grading the severity of postoperative complications in global surgery research

Background The Clavien–Dindo classification is perhaps the most widely used approach for reporting postoperative complications in clinical trials. This system classifies complication severity by the treatment provided. However, it is unclear whether the Clavien–Dindo system can be used internationally in studies across differing healthcare systems in high- (HICs) and low- and middle-income countries (LMICs). Methods This was a secondary analysis of the International Surgical Outcomes Study (ISOS), a prospective observational cohort study of elective surgery in adults. Data collection occurred over a 7-day period. Severity of complications was graded using Clavien–Dindo and the simpler ISOS grading (mild, moderate or severe, based on guided investigator judgement). Severity grading was compared using the intraclass correlation coefficient (ICC). Data are presented as frequencies and ICC values (with 95 per cent c.i.). The analysis was stratified by income status of the country, comparing HICs with LMICs. Results A total of 44 814 patients were recruited from 474 hospitals in 27 countries (19 HICs and 8 LMICs). Some 7508 patients (16·8 per cent) experienced at least one postoperative complication, equivalent to 11 664 complications in total. Using the ISOS classification, 5504 of 11 664 complications (47·2 per cent) were graded as mild, 4244 (36·4 per cent) as moderate and 1916 (16·4 per cent) as severe. Using Clavien–Dindo, 6781 of 11 664 complications (58·1 per cent) were graded as I or II, 1740 (14·9 per cent) as III, 2408 (20·6 per cent) as IV and 735 (6·3 per cent) as V. Agreement between classification systems was poor overall (ICC 0·41, 95 per cent c.i. 0·20 to 0·55), and in LMICs (ICC 0·23, 0·05 to 0·38) and HICs (ICC 0·46, 0·25 to 0·59). Conclusion Caution is recommended when using a treatment approach to grade complications in global surgery studies, as this may introduce bias unintentionally