Living with congenital heart disease

Congenital heart defects, affecting 9.4 out of 1000 newborns, represent the most prevalent congenital diseases. Advances in medical and surgical interventions have significantly increased the survival rate, with over 90% of affected individuals now reaching adulthood. This study focuses on a cohort of patients who underwent surgical corrections in Rotterdam between 1968 and 1980, offering insights into their quality of life 40-53 years post-surgery, with a specific emphasis on psychosocial aspects. This research, focusing on the psychopathological aspects of adults with congenital heart disease, reveals a temporal decrease in psychopathology, though an increase in the last decade is noted. Gender differences were underlined in psychological well-being, with women experiencing more internalizing problems, while men exhibit fewer externalizing problems compared to their peers. Interestingly, adults with congenital heart disease do not demonstrate lower executive functions, but are more likely to have lower educational and occupational levels than the general population, especially in higher professions.Additionally, adults with congenital heart defects show reduced involvement in sports, irrespective of the severity of their condition. However, engagement in physical activity correlates with better psychological well-being. Moreover, they perceive their life expectancy similarly to, or even more positively than, their healthy peers. Furthermore, they show reluctance to engage in end-of-life discussions with physicians. Finally, this thesis highlights the favourable cardiological long-term prognosis for individuals with atrial septal defect, a relatively uncomplicated congenital heart defect.In summary, this thesis contributes robust data and valuable insights into the long-term development and evolving quality of life of adults with congenital heart diseases, from surgical intervention to adulthood.<br/

Safety Compliance in a Sample of Italian Mechanical Companies: The Role of Knowledge and Safety Climate

The accident rate in the Italian mechanical sector is still too high, and evidence-based interventions to improve safety performance are essential. To better address this, our study contributes to the understanding of how to promote safety compliance through safe behaviours by using a sample of Italian mechanical workers (n = 109). Before and after scheduled safety training, intervention data on organizational factors, as well as on individual factors affecting safety-related behaviours, were collected. Particularly, data were collected using multiple sources, including self-perception questionnaires (to measure the safety climate among the management and colleagues and the safety attitude), paper and pencil tests (to measure safety knowledge), and observations by personnel with experience in observation tasks (to measure safety behaviours objectively). A model class of competing general linear models was built to determine which of the models was best suited for predicting safety-related behaviours. The results showed that both knowledge and the management’s safety climate effectively promoted safety compliance. Crucial implications for the effectiveness of active teaching methods, along with the need for continuous training and the prominent role of the management team members in giving, through their actions, further relevance to the need to respect rules and procedures, were revealed. Finally, practical implications for researchers, corporate decision makers, government agencies, and international bodies are discussed

Main-chain water-soluble polyphosphoesters: multi-functional polymers as degradable PEG-alternatives for biomedical applications

Polyphosphoesters (PPEs) are a class of (bio)degradable polymers with high chemical versatility and functionality. In particular, water-soluble PPEs with the phosphoester group in the polymer backbone are currently discussed as a potential alternative to poly(ethylene glycol) (PEG). Ring-opening polymerization of typically 5-membered cyclic phosphoesters gives straightforward access to various well-defined PPEs. Several PPE candidates have proven their biocompatibility in vitro in terms of cytocompatibility, antifouling properties, “stealth effect”, degradability (hydrolytic and enzymatic), and some promising in vivo results in drug delivery vehicles. The possibility to control the properties with the appropriate tuning of the lateral chain makes PPEs especially appealing. This review summarizes recent developments of such PPEs for biomedical applications, e.g. in protein-polymer conjugates, hydrogels for tissue engineering, or nanocarriers for drug and gene delivery. We summarize the progress made over the years, highlighting the strengths and the shortcomings of PPEs for these applications to date. We critically evaluate the current state of the art, try to assess their potential and to predict future perspectives, shedding light on the pathway that needs to be followed to translate into clinics

Effectiveness of occupational health and safety training. A systematic review with meta-analysis

Purpose This meta-analysis aims to verify the efficacy of occupational health and safety (OHS) training in terms of knowledge, attitude and beliefs, behavior and health. Design/methodology/approach The authors included studies published in English (2007–2014) selected from ten databases. Eligibility criteria were studies concerned with the effectiveness of OHS training for primary prevention of workplace injury; and studies focused on examined outcome related to OHS. Findings The selected studies (n = 28) highlighted a strong support for the effectiveness of training on worker OHS attitudes and beliefs and, to a lesser extent, on worker’s knowledge but only medium for behavior and small evidences for its effectiveness on health. Research limitations/implications Future research should more deeply investigate the efficacy on knowledge increase of trainings delivered by experts and researchers, applying different methods, in a small group; training delivered by peer and by researcher, applying different methods; and trained workers less than 29 years and more than 49 years old, considering that workers in these age groups are particularly vulnerable to fatalities. Practical implications Our study is a contribution for those they intend to grant effective training, in response to specific needs of OHS. The evidences presented could be considered a first step to identify the factors related to the efficacy of OHS training to plan adequate interventions. Social implications The OHS training is effective on the basis of the extent interventions are carried out for each specific learning outcome. Originality/value This meta-analysis suggested that classroom training, although the most used and studied, does not ever revealed itself very effective: it was not significant for outcomes in terms of knowledge and showed a decreasing efficacy for attitudes and beliefs, behaviors and health. It seemed that there was a distinction between interventions on knowledge, attitudes and beliefs, as opposed to behavioral interventions and health

Synthesis, thermal, and mechanical characterisation of metakaolin-based geopolymers coloured with grape marc extract

Over the years, several materials have been used for restoration purposes, with different types of dyes and colour hues. Recently, some researchers have proposed geopolymers (GPs) or amorphous aluminosilicate polymers for these purposes. In this work, an alcohol-based grape marc extract (GME, obtained via dark maceration assisted with ultrasound) was used as a natural dyeing agent for metakaolin-based GPs. The geopolymerisation occurrence was assessed by Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffraction analysis, while the colour of the resulting material was determined through the colorimetric analysis in the L*a*b* colour space. Additionally, the thermal stability of GME and GPs was investigated by thermogravimetry coupled with FT-IR spectroscopy. The microstructure, the reticulation stability, and the antimicrobial activity of GPs were examined through the scanning electron microscopy, the pH and ionic conductivity measurements, integrity, and mass loss tests. Overall, a coloured geopolymer with suitable thermal, antimicrobial, and mechanical properties was obtained, justifying its potential use in restoration or, more generally, in the construction field

Clinical significance of inflammatory markers of bacterial infection in critically ill patients with COVID-19 after treatment with anti-inflammatory and immunomodulatory drugs: a complex new scenario.

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Audience effect on domestic dogs’ behavioural displays and facial expressions

In the present study we investigated the infuence of positive and negative arousal situations and the presence of an audience on dogs’ behavioural displays and facial expressions. We exposed dogs to positive anticipation, non-social frustration and social frustration evoking test sessions and measured pre and post-test salivary cortisol concentrations. Cortisol concentration did not increase during the tests and there was no diference in pre or post-test concentrations in the diferent test conditions, excluding a diferent level of arousal. Displacement behaviours of “looking away” and “snifng the environment” occurred more in the frustration-evoking situations compared to the positive anticipation and were correlated with cortisol concentrations. “Ears forward” occurred more in the positive anticipation condition compared to the frustration-evoking conditions, was positively infuenced by the presence of an audience, and negatively correlated to the pre-test cortisol concentrations, suggesting it may be a good indicator of dogs’ level of attention. “Ears fattener”, “blink”, “nose lick”, “tail wagging” and “whining” were associated with the presence of an audience but were not correlated to cortisol concentrations, suggesting a communicative component of these visual displays. These fndings are a frst step to systematically test which subtle cues could be considered communicative signals in domestic dogs

Thermodynamic stability of myoglobin-poly(ethylene glycol) bioconjugates: A calorimetric study

PEGylated proteins are widely used for therapeutic applications, therefore a fundamental understanding of the conjugates’ structure and their behaviour in solution is essential to promote new developments in this field. In the present work, myoglobin-poly(ethylene glycol) conjugates were synthesized and studied by differential scanning calorimetry and UV–vis spectroscopy to obtain information on the bioconjugates’ thermodynamic stability, also focusing on PEG’s role on the solvent-protein surface interaction. The overall results of this study indicated a thermal destabilization of the protein that follows the extent of the bioconjugation without, however, compromising the native structure which remains functional. Moreover, the myoglobin PEGylation prevented the post-denaturation aggregation phenomena and enhanced the protein thermal reversibility. The thermodynamic interpretation of the data indicated that the bioconjugation influences the solvent-exposed protein surface difference between native and denatured state, contributing to the interpretation of the overall protein modification and functionality

Thermodynamic Evaluation of the Interactions between Anticancer Pt(II) Complexes and Model Proteins

In this work, we have analysed the binding of the Pt(II) complexes ([PtCl(4′-phenyl-2,2′:6′,2″-terpyridine)](CF3SO3) (1), [PtI(4′-phenyl-2,2′:6′,2″-terpyridine)](CF3SO3) (2) and [PtCl(1,3-di(2-pyridyl)benzene) (3)] with selected model proteins (hen egg-white lysozyme, HEWL, and ribonuclease A, RNase A). Platinum coordination compounds are intensively studied to develop improved anticancer agents. In this regard, a critical issue is the possible role of Pt-protein interactions in their mechanisms of action. Multiple techniques such as differential scanning calorimetry (DSC), electrospray ionization mass spectrometry (ESI-MS) and UV-Vis absorbance titrations were used to enlighten the details of the binding to the different biosubstrates. On the one hand, it may be concluded that the affinity of 3 for the proteins is low. On the other hand, 1 and 2 strongly bind them, but with major binding mode differences when switching from HEWL to RNase A. Both 1 and 2 bind to HEWL with a non-specific (DSC) and non-covalent (ESI-MS) binding mode, dominated by a 1:1 binding stoichiometry (UV-Vis). ESI-MS data indicate a protein-driven chloride loss that does not convert into a covalent bond, likely due to the unfavourable complexes’ geometries and steric hindrance. This result, together with the significant changes of the absorbance profiles of the complex upon interaction, suggest an electrostatic binding mode supported by some stacking interaction of the aromatic ligand. Very differently, in the case of RNase A, slow formation of covalent adducts occurs (DSC, ESI-MS). The reactivity is higher for the iodo-compound 2, in agreement with iodine lability higher than chlorine

A circular dichroism study of the protective role of polyphosphoesters polymer chains in polyphosphoester‐myoglobin conjugates

Protein-polymer conjugates are a blooming class of hybrid systems with high biomedical potential. Despite a plethora of papers on their biomedical properties, the physical–chemical characterization of many protein-polymer conjugates is missing. Here, we evaluated the thermal stability of a set of fully-degradable polyphosphoester-protein conjugates by variable temperature circular dichroism, a common but powerful technique. We extensively describe their thermodynamic stability in different environments (in physiological buffer or in presence of chemical denaturants, e.g., acid or urea), highlighting the protective role of the polymer in preserving the protein from denaturation. For the first time, we propose a simple but effective protocol to achieve useful information on these systems in vitro, useful to screen new samples in their early stages