Experimentally Based Numerical Simulation of the Influence of the Agricultural Subsurface Drainage Pipe Geometric Structure on Drainage Flow

The geometric structure of corrugated plastic pipes affects performance in agricultural subsurface drainage systems. To explore the influence of pipe geometry on flow field characteristics and the characterization of water movements, we developed a three-dimensional (3D) steady-state subsurface drainage model based on computational fluid dynamics (CFD). An analysis of the CFD and sand tank results indicated that the proposed model can accurately simulate the subsurface drainage process (R2 = 0.99). The corrugation structure parameters of the drainpipe, including the outside diameter, corrugation valley width and corrugation height, were taken as the objects for this study, and the influence of corrugation parameters on drainage discharge was orthogonally analysed. During drainage, the soil water initially collects in the corrugation valley and then approximately ninety percent of the water flows into the pipe through the bottom perforations; increasing the contact face area between the corrugation valley and soil can increase the flow rate of the drainpipe and the water table height above the pipe, which decreases the intersection position of the pipe and water table. The results of the analysis of the range and variance of the orthogonal experiment showed that the order of the primary and secondary factors influencing the drainage discharge was the outside diameter, corrugation valley width and corrugation height, with the outside diameter being most critical influencing factor

Influence of Sediment, Plants, and Microorganisms on Nitrogen Removal in Farmland Drainage Ditches

The removal of nitrogen from water is a consequence of the synergistic action of plant uptake, sediment sorption, and microbial decomposition. However, there is a lack of long-term experimental studies on the effects of each component in the process of nitrogen removal. In this study, we investigated the effect of sediment, plants, and microorganisms on nitrogen removal by setting up three systems: water–sterilized sediment, water–sediment, and water–sediment–plant. The nitrogen removal effect followed the following rank order of effectiveness: the “water–sediment–plant” system > the “water–sediment” system > the “water–sterilized sediment” system. The ditch sediment had a strong enrichment effect for nitrogen. In addition, the migration rate of nitrogen in the sediment with different depths was different. The ammonia-nitrogen migration rate in the sediment showed an increasing trend with time and depth. The nitrate-nitrogen migration process in the sediment showed a trend of enrichment toward the middle layer (15.0–25.0 cm). Aquatic plants and microorganisms can promote the removal of nitrogen in water, with the average purification rates of 13.92% and 19.92%, respectively

Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and β‐alanine metabolism

Summary Mevalonate (MVA) pathway is the core for terpene and sterol biosynthesis, whose metabolic flux influences the synthesis efficiency of such compounds. Saccharomyces cerevisiae is an attractive chassis for the native active MVA pathway. Here, the truncated form of Enterococcus faecalis MvaE with only 3‐Hydroxy‐3‐methylglutaryl coenzyme A reductase (HMGR) activity was found to be the most effective enzyme for MVA pathway flux using squalene as the metabolic marker, resulting in 431‐fold and 9‐fold increases of squalene content in haploid and industrial yeast strains respectively. Furthermore, a positive correlation between MVA metabolic flux and β‐alanine metabolic activity was found based on a metabolomic analysis. An industrial strain SQ3‐4 with high MVA metabolic flux was constructed by combined engineering HMGR activity, NADPH regeneration, cytosolic acetyl‐CoA supply and β‐alanine metabolism. The strain was further evaluated as the chassis for terpenoids production. Strain SQ3‐4‐CPS generated from expressing β‐caryophyllene synthase in SQ3‐4 produced 11.86 ± 0.09 mg l−1 β‐caryophyllene, while strain SQ3‐5 resulted from down‐regulation of ERG1 in SQ3‐4 produced 408.88 ± 0.09 mg l−1 squalene in shake flask cultivations. Strain SQ3‐5 produced 4.94 g l−1 squalene in fed‐batch fermentation in cane molasses medium, indicating the promising potential for cost‐effective production of squalene

Effects of Nitrogen Input and Aeration on Greenhouse Gas Emissions and Pollutants in Agricultural Drainage Ditches

Understanding the patterns of greenhouse gas emissions and the changes in pollution load in terrestrial freshwater systems is crucial for accurately assessing the global carbon cycle and overall greenhouse gas emissions. However, current research often focuses on wetlands and rivers, with few studies on agricultural drainage ditches, which are an important part of the agricultural ecosystem. Investigating the greenhouse gas emission patterns and pollution load changes in agricultural drainage ditches can help accurately assess the greenhouse effect of agricultural systems and improve fertilization measures in farmlands. This study explored the effects of nitrogen input and aeration on the pollution load and greenhouse gas emission processes in paddy field drainage ditches. The results showed that aeration significantly reduced the concentration of ammonium nitrogen (NH4+) in the water, decreased the emissions of nitrous oxide (N2O) and methane (CH4), and slightly increased the emission of carbon dioxide (CO2), resulting in an overall reduction of the global warming potential (GWP) by 34.02%. Nitrogen input significantly increased the concentration of ammonium nitrogen in the water, slightly reduced the emissions of N2O and CH4, and increased the CO2 emissions by 46.60%, thereby increasing the GWP by 15.24%. The drainage ditches reduced the pollution load in both the water and sediment, with the overall GWP downstream being 9.34% lower than upstream

Critical Factors Affecting Water and Nitrogen Losses from Sloping Farmland during the Snowmelt Process

Water and nitrogen losses from farmland during the snowmelt process play a vital role in water and nitrogen management in cold regions. To explore the mechanisms and factors contributing to water and nitrogen loss from different sloping farmlands during the snowmelt period, field experiments were conducted under two slope treatments (8° and 15°), two soil water content (SWC) treatments, and two snow water equivalent (SWE) (5 mm and 10 mm) treatments in a seasonal freezing agricultural watershed of Northeast China. The results showed that during the snowmelt process, SWE was the most important factor affecting water and nitrogen production through the surface and total runoff of the sloping farmland, followed by the slope. The water and nitrogen yield in the high snow (HS) treatments ranged from 1.76 to 8.15 and 1.65 to 12.62 times higher than those in the low snow (LS) treatments. The generation of nitrogen was advanced compared with that of water induced by the preferential production of nitrogen. A higher slope promoted this preferential production function of nitrogen. Enhanced infiltration combined with the preferential yield of nitrogen resulted in a greatly decreased yield of water and nitrogen in the gentle slope and LS (GS_LS) treatments. These findings are valuable for accurately describing the water and nitrogen cycling in seasonally freezing sloping farmland

Effects of Freezing–Thawing Processes on Net Nitrogen Mineralization in Salinized Farmland Soil

Nitrogen is an indispensable and limiting element for plant and microbial growth. To investigate the combined effects of salinity and freezing–thawing (FT) processes on soil inorganic nitrogen (SIN) transformation in seasonally freezing salinized farmland, laboratory incubation experiments were conducted under five soil salt content (SSC) treatments (0.08%, 0.25%, 0.35%, 0.50%, and 0.70%), four FT temperature treatments (C (5 °C), FT (−5 + 5 °C), FT (−10 + 5 °C), and FT (−15 + 5 °C)), and two soil water content (SWC) treatments (40% and 80% of maximum water holding capacity (WHC)). Ammonium (NH4+-N) and nitrate (NO3−-N) nitrogen were monitored at the first, second, fifth, and eighth incubation days. The FT processes increased relative NH4+-N content by 13%, 39%, and 77% with the decreasing of freezing temperature from −5 °C to −15 °C compared with C (5 °C) treatments, respectively. FT (−5 + 5 °C) and FT (−15 + 5 °C) treatments decreased the relative NO3--N contents by 4% and 6% compared with C (5 °C) treatments, respectively. Under FT treatments, the increment of relative NH4+-N content was higher in low-SSC treatments and lower in high-SSC treatments. The relationship between relative NO3–-N content and SSC gradually changed from a decrease in C (5 °C) to an increase in FT (−15+5 °C) treatments. SWC decreased NH4+-N content in high-SSC and low-freezing temperature treatments (SSC × freezing temperature 4+-N increased in low-SSC and unfrozen treatments. The variations of SIN/Rmin (nitrogen mineralization rate) were mostly affected by NO3–-N/Rnit (net nitrification rate) and NH4+-N/Ra (net ammonification rate) in C (5 °C) and FT treatments, respectively. Overall, the results suggested that enhanced salinity inhibited the effects of freezing temperature on NH4+-N and NO3−-N formation, respectively. The increase in SWC weakened the NH4+-N formation induced by the decrease in freezing temperature, and this function increased with the increase in salinity

Effects of Aeration on Pollution Load and Greenhouse Gas Emissions from Agricultural Drainage Ditches

Human activities input a large amount of carbon and nitrogen nutrients into water, resulting in inland freshwater becoming an important source of greenhouse gas (GHG) emissions. Agricultural drainage ditches are the main transport route of non-point source pollution. Understanding the rules for how greenhouse gas emissions from drainage ditches impact the environment can help to accurately estimate the greenhouse effect of agricultural systems. However, current research mainly focuses on the effect of different measures on the migration and transformation process of pollutants in drainage ditches. The process of greenhouse gas emissions when the non-point source of pollution is transported by drainage ditches is still unclear. In this study, the influence of aeration on the pollution load and GHG emission process of a drainage ditch in a paddy field was explored. The following conclusions were drawn: Aeration reduced the content of nitrate nitrogen in the water but had no significant effect on the content of ammonium nitrogen and it reduced the chemical oxygen demand (COD) of water by 24.9%. Aeration increased the potential of hydrogen (PH), dissolved oxygen (DO) and oxidation–reduction potential (ORP) of water and reduced the total organic carbon content, microbial carbon content and soluble carbon content of the soil in the sediment. Aeration reduced the N2O and CH4 emission fluxes and increased the CO2 emission fluxes in the drainage ditch, but it reduced the greenhouse effect generated by the drainage ditch by 33.7%. This study shows that aeration can reduce both the pollution load and the greenhouse gas emission flux in drainage ditches