Low temperature, mechanical wound, and exogenous salicylic acid (SA) can stimulate the SA signaling molecule as well as its downstream pathway and the formation of fruiting bodies in Flammulina filiformis

Low temperature (LT) and mechanical wound (MW), as two common physics methods, have been empirically used in production to stimulate the primordia formation of Flammulina filiformis, which is typically produced using the industrial production mode. However, the detailed effect on the fruiting body formation and important endogenous hormones and signaling pathways in this process is poorly understood. In this study, LT, MW, their combination, i.e., MW + LT, and low concentration of SA (0.1 mM SA) treatments were applied to the physiologically mature mycelia of F. filiformis. The results showed that the primordia under the four treatments began to appear on the 5th−6th days compared with the 12th day in the control (no treatment). The MW + LT treatment produced the largest number of primordia (1,859 per bottle), followed by MW (757), SA (141), and LT (22), compared with 47 per bottle in the control. The HPLC results showed that the average contents of endogenous SA were significantly increased by 1.3 to 2.6 times under four treatments. A total of 11 SA signaling genes were identified in the F. filiformis genome, including 4 NPR genes (FfNpr1-4), 5 TGA genes (FfTga1-5), and 2 PR genes (FfPr1-2). FfNpr3 with complete conserved domains (ANK and BTB/POZ) showed significantly upregulated expression under all four above treatments, while FfNpr1/2/4 with one domain showed significantly upregulated response expression under the partial treatment of all four treatments. FfTga1-5 and FfPr1-2 showed 1.6-fold to 8.5-fold significant upregulation with varying degrees in response to four treatments. The results suggested that there was a correlation between “low temperature/mechanical wound—SA signal—fruiting body formation”, and it will help researchers to understand the role of SA hormone and SA signaling pathway genes in the formation of fruiting bodies in fungi

PSR J1926-0652: A Pulsar with Interesting Emission Properties Discovered at FAST

We describe PSR J1926-0652, a pulsar recently discovered with the Five-hundred-meter Aperture Spherical radio Telescope (FAST). Using sensitive single-pulse detections from FAST and long-term timing observations from the Parkes 64-m radio telescope, we probed phenomena on both long and short time scales. The FAST observations covered a wide frequency range from 270 to 800 MHz, enabling individual pulses to be studied in detail. The pulsar exhibits at least four profile components, short-term nulling lasting from 4 to 450 pulses, complex subpulse drifting behaviours and intermittency on scales of tens of minutes. While the average band spacing P3 is relatively constant across different bursts and components, significant variations in the separation of adjacent bands are seen, especially near the beginning and end of a burst. Band shapes and slopes are quite variable, especially for the trailing components and for the shorter bursts. We show that for each burst the last detectable pulse prior to emission ceasing has different properties compared to other pulses. These complexities pose challenges for the classic carousel-type models.Comment: 13pages with 12 figure

Direct and indirect effects of climate on richness drive the latitudinal diversity gradient in forest trees

Data accessibility statement: Full census data are available upon reasonable request from the ForestGEO data portal, http://ctfs.si.edu/datarequest/ We thank Margie Mayfield, three anonymous reviewers and Jacob Weiner for constructive comments on the manuscript. This study was financially supported by the National Key R&D Program of China (2017YFC0506100), the National Natural Science Foundation of China (31622014 and 31570426), and the Fundamental Research Funds for the Central Universities (17lgzd24) to CC. XW was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB3103). DS was supported by the Czech Science Foundation (grant no. 16-26369S). Yves Rosseel provided us valuable suggestions on using the lavaan package conducting SEM analyses. Funding and citation information for each forest plot is available in the Supplementary Information Text 1.Peer reviewedPostprin

Allele-Specific Transcriptional Regulation of Shoot Regeneration in Hybrid Poplar

Plant tissue regeneration is a key process for genetic transformation and genome editing. The exploration of regulatory mechanisms in plant regeneration would improve regeneration efficiency. In comparison to some model plants, the genomic heterozygosity is much higher in forest trees, increasing the complexity of transcriptional regulation. Here, we report the allele-specific transcriptional analysis in hybrid poplar 84K (Populus alba × P. tremula var. glandulosa cv. 84K) during the shoot regeneration process. Firstly, 180 regeneration-related genes (REGs) and 2446 REG-homologous genes (REGHs) were identified in hybrid poplar. The expression patterns of REGs exhibited that about half of them were positively correlated between poplar and Arabidopsis at the locus level. The expression levels of REGHs vary among the gene family at different stages during callus and shoot induction. Among the gene clusters with similar expression patterns, the distribution of gene families in poplar and Arabidopsis also exhibits large variations. At the allele level, most of the allele pairs of REGs were positively correlated in expression. The expression patterns of genes in auxin synthesis, transport, and signaling pathways agree with the general patterns. Due to the presence/absence of variations between two subgenomes, two YUC alleles and two IAA alleles are present only in one subgenome, and the expression patterns of the two alleles are greatly different. Our analysis indicates the conservativeness and diversity of transcriptional regulation during shoot regeneration in poplar and Arabidopsis. The complexity in allele expression contributed by heterozygosity suggests the importance of genotyping in the screening of explants for plant regeneration

Temporal effects of disturbance on community composition in simulated stage-structured plant communities

© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. In an era of global environmental change, understanding how disturbance affects the dynamics of ecological communities is crucial. However, few studies have theoretically explored the potential influence of disturbance including both intensity and frequency on compositional change over time in communities with stage structure. A spatially explicit, individual-based model was constructed incorporating the various demographic responses to disturbance of plants at two different growth stages: seedlings and adults. In the model, we assumed that individuals within each stage were demographically equivalent (neutral) but differed between stages. We simulated a common phenomenon that seedlings suffered more from disturbance such as grazing and fire than adults. We showed how stage-structured communities of seedlings and adults responded to disturbance with various levels of disturbance frequency and intensity. In “undisturbed” simulations, the relationship between average species abundance (defined here as the total number of individuals divided by species richness) and community composition turnover (measured by the Bray–Curtis similarity index) was asymptotic. However, in strongly “disturbed” simulations with the between-disturbance intervals greater than one, this relationship became unimodal. Stage-dependent response to disturbance underlay the above discrepancy between undisturbed and disturbed communities

Mapping Potential Soil Water Erosion and Flood Hazard Zones in the Yarlung Tsangpo River Basin, China

The ubiquity of soil water erosion in the Yarlung Tsangpo River Basin leads to a series of natural hazards, including landslides, debris flows and floods. In this study, the Revised Universal Soil Loss Equation model (RUSLE) was used to quantify potential soil water erosion, while the Height Above Nearest Drainage model (HAND) was used to delimit potential flood hazard zones. Remote sensing and geographic information system technologies were employed to spatialize the results, which showed that the annual soil loss from water erosion was less than 1239 t ha−1 y−1. The total soil loss was estimated to be over 108 × 106 tons, of which about 13 × 106 tons (12.04% of the total) occurred from the agricultural land in the downstream valley. Soil erosion mapping was performed using six levels of soil erosion intensity and the effects of precipitation, land use/land cover and topography on soil erosion were revealed. Increases in precipitation and slope gradient significantly increased the soil loss rate, while the maximum rate of soil loss occurred from densely vegetated land, reaching 9.41 t ha−1 y−1, which was inconsistent with erosion preconceptions for this land type. This may be due to a combination of the region’s unique climate of high intensity rainfall and steep slopes. Flood hazard mapping showed that all regional cities were located in a flood hazard zone and that, within the total basin area (~258 × 105 ha), 9.84% (2,537,622 ha) was in a high flood occurrence area, with an additional 1.04% in aa vulnerable to moderate flood hazard area. Approximately 1.54% of the area was in a low flood risk area and 4.15% was in a very low flood risk area. The results of this study provide an initial identification of high-risk soil water erosion and flood hazard locations in the basin and provide a foundation upon which decision-makers can develop water and soil conservation and flood prevention policies

Soil microbial biomass increases along elevational gradients in the tropics and subtropics but not elsewhere

Aim: Our aim is to use elevational gradients to quantify the relationship between temperature and ecosystem functioning. Ecosystem functions such as decomposition, nutrient cycling and carbon storage are linked with the amount of microbial biomass in the soil. Previous studies have shown variable relationships between elevation and soil microbial biomass (SMB). Understanding the biological mechanisms linking SMB with elevational gradients will shed light on the environmental impacts of global warming. Location: Global. Time period: 2002–2018. Major taxa studied: Soil microbes. Method: We performed a global meta-analysis of the relationships between SMB and elevation. Data were collected from 59 studies of 73 elevational transects from around the world. Results: We found no consistent global relationship between SMB and elevation. SMB increased significantly with elevation in the tropics and subtropics, but not in other climate zones. However, we found consistent positive relationships between SMB, soil organic carbon and total nitrogen concentrations. Main conclusions: Our results suggest that global warming will impact tropical and subtropical ecosystems more severely than colder regions. Tropical ecosystems, already at risk from species extinctions, will likely experience declines in SMB as the climate warms, resulting in losses of fundamental ecosystem functions such as nutrient cycling and carbon storage