Bicriterion scheduling with equal processing times on a batch processing machine

Author name used in this publication: C. T. NgAuthor name used in this publication: T. C. E. Cheng2008-2009 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Single-machine scheduling with a time-dependent learning effect

Author name used in this publication: J.-B. WangAuthor name used in this publication: C. T. NgAuthor name used in this publication: T. C. E. Cheng2007-2008 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Purification and biochemical characterization of a serine alkaline protease TC4 from a new isolated Bacillus alcalophilus TCCC11004 in detergent formulations

An extracellular alkaline protease producing strain was isolated from alkaline soil and identified as Bacillus alcalophilus TCCC11004 on the basis of 16S rDNA gene sequencing and biochemical properties. The most appropriate medium for the protease production was composed of (g/l): maltodextrin 110, yeast extract 17.5, cotton seed meal 29.3, K2HPO4 18, trisodium citrate 3.3 and CaCl2 2.6. The alkaline protease TC4 was purified from the culture supernatant by ammonium sulfate precipitation, Sephadex G-75 gel filtration and SP-Sepharose HP ion exchange chromatography, with a 6.8 fold increase in specific activity and 15.2% recovery. The molecular weight was estimated to be 26 kDa on SDS-PAGE. The protease was highly active from pH 9.0-12.0 with an optimal at pH 11.0. It was active at 30 - 60°C and exhibited maximal activity at 50°C. The thermostability of the protease was increased by the addition of CaCl2. It retained 70 and 81% of its initial activity after heating for 2 h at 50°C, in the absence or presence of 2 mM CaCl2, respectively. The enzyme was inactivated by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, suggesting that it is a serine protease. The protease was stable in 0.5% SDS and retained 70.3% of its initial activity after 1 h of incubation. It was active in the presence of 3% Triton X-100 with 100% activity and stable towards oxidizing agent with 69.2% activity in the presence of 1% H2O2. The enzyme showed excellent compatibility with commercial detergents such as TaiZi, BiLang, DiaoPai and TianQing, retaining more than 90% of its initial activity in the tested detergents after 1 h of preincubation at 40°C.Keywords: Serine alkaline protease, Bacillus alcalophilus, stability, detergent compatibility

The Influence of Accumulated Precipitation on Debris Flow Hazard

Debris flow warning system in Taiwan uses effective accumulated precipitation as the warning criteria. Little research has studied the affected area associated with different levels of accumulated precipitation. The Taipei DF024 potential debris flow torrent is used as an example to study the relation between an affected area and accumulated precipitation. Three different amount of accumulated precipitation 300, 500 (official warning criteria) and 700 mm in 24 hours are chosen for comparison. Average rainfall intensity per hour is calculated from accumulated rainfall through the Monobe formula. Total water flow rate is estimated using the Rational formula and a discharge hydrograph. Maximum debris flow volume is determined by the equilibrium concentration formula. All sources of debris are distributed on landslide areas and river bed from field investigation. DEBRIS-2D is used to simulate debris flows. The results show that the hazard area is proportional to precipitation, and the thickness of maximum debris flow accumulation is between 3 and 3.5 m for all three cases. The relation between accumulated precipitation and hazard area can provide officials with additional information related to resident evacuation.土石流預警在台灣是以有效累積雨量為基準，但在不同累積雨量下的土石流災害範圍，卻少有學者討論。針對這個問題，本文以北市DF024 為例，用其警戒雨量500 毫米做為基準，再選300 和700 毫米的累積雨量做影響範圍的比較。透過物部公式 (Monobe formula) 將累積雨量轉換為降雨強度，並搭配合理化公式和流量歷線去估算集水區產生的總水量，再以土石流平衡濃度公式推估土石流體積量。並經過現場調查，將料源分佈於崩坍地與河床崩積層，最後採用DEBRIS-2D 去模擬土石流影響範圍。在北市DF024 的案例中發現，土石流影響範圍會隨著累積雨量的增加而擴大，但最大土石堆積深度皆在3~3.5 公尺之間。透過此方法得到的土石流影響範圍不只可以連結與累積雨量的關係，並且可提供相關單位一個修正警戒雨量或疏散 範圍決策的參考

Environment-specific combinatorial cis-regulation in synthetic promoters

When a cell's environment changes, a large transcriptional response often takes place. The exquisite sensitivity and specificity of these responses are controlled in large part by the combinations of cis-regulatory elements that reside in gene promoters and adjacent control regions. Here, we present a study aimed at accurately modeling the relationship between combinations of cis-regulatory elements and the expression levels they drive in different environments. We constructed four libraries of synthetic promoters in yeast, consisting of combinations of transcription factor binding sites and assayed their expression in four different environments. Thermodynamic models relating promoter sequences to their corresponding four expression levels explained at least 56% of the variation in expression in each library through the different conditions. Analyses of these models suggested that a large fraction of regulated gene expression is explained by changes in the effective concentration of sequence-specific transcription factors, and we show that in most cases, the corresponding transcription factors are expressed in a pattern that is predicted by the thermodynamic models. Our analysis uncovered two binding sites that switch from activators to repressors in different environmental conditions. In both the cases, the switch was not the result of a single transcription factor changing regulatory modes, but most likely due to competition between multiple factors binding to the same site. Our analysis suggests that this mode of regulation allows for large and steep changes in expression in response to changing transcription factor concentrations. Our results demonstrate that many complex changes in gene expression are accurately explained by simple changes in the effective concentrations of transcription factors

Growth suppressive effect of pegylated arginase in malignant pleural mesothelioma xenografts

BACKGROUND: Malignant pleural mesothelioma (MPM) is a difficult-to-treat global disease. Pegylated arginase (BCT-100) has recently shown anti-tumor effects in hepatocellular carcinoma, acute myeloid leukemia and melanoma. This study aims to investigate the effects of PEG-BCT-100 in MPM. METHODS: A panel of 5 mesothelioma cell lines (H28, 211H, H226, H2052 and H2452) was used to study the in vitro effects of BCT-100 by crystal violet staining. The in vivo effects of BCT-100 were studied using 211H and H226 nude mice xenografts. Protein expression (argininosuccinate synthetase, ornithine transcarbamylase, cleaved PARP, cleaved caspase 3, cyclins (A2, D3, E1 and H), CDK4 and Ki67) and arginine concentration were evaluated by Western blot and ELISA respectively. Cellular localization of BCT-100 was detected by immunohistochemistry and immunoflorescence. TUNEL assay was used to identify cellular apoptotic events. RESULTS: Argininosuccinate synthetase was expressed in H28, H226, and H2452 cells as well as 211H and H266 xenografts. Ornithine transcarbamylase was undetectable in all cell lines and xenograft models. BCT-100 reduced in vitro cell viability (IC50 values at 13-24 mU/ml, 72 h) across different cell lines and suppressed tumor growth in both 211H and H226 xenograft models. BCT-100 (60 mg/kg) significantly suppressed tumor growth (p < 0.01) with prolonged median survival (p < 0.01) in both xenograft models. Combining BCT-100 with pemetrexed or cisplatin conferred no additional benefits over single agents. Serum and intratumoral arginine levels were effectively decreased by BCT-100, associated with cytosolic accumulation of BCT-100 within tumor cells. Apoptosis (PARP cleavage in 211H xenografts; Bcl-2 downregulation, and cleavage of PARP and caspase 3 in H226 xenografts; positive TUNEL staining in both) and G1 arrest (downregulation of cyclin A2, D3, E1 and CDK4 in 211H xenografts; suppression of cyclin A2, E1, H and CDK4 in H226 xenografts) were evident with BCT-100 treatment. Furthermore, proliferative factor Ki67 was downregulated in BCT-100 treatments arms. CONCLUSIONS: BCT-100 suppressed tumor growth with prolonged median survival partially mediated by intratumoral arginine depletion resulting in apoptosis and G1 arrest in mesothelioma xenograft models. The findings provide scientific evidence to support further clinical development of BCT-100 in treatment of MPM.published_or_final_versio

Flavor SU(3) symmetry and QCD factorization in B→PPB \to PP and PVPV decays

Using flavor SU(3) symmetry, we perform a model-independent analysis of charmless $\bar B_{u,d} (\bar B_s) \to PP, ~PV$ decays. All the relevant topological diagrams, including the presumably subleading diagrams, such as the QCD- and EW-penguin exchange diagrams and flavor-singlet weak annihilation ones, are introduced. Indeed, the QCD-penguin exchange diagram turns out to be important in understanding the data for penguin-dominated decay modes. In this work we make efforts to bridge the (model-independent but less quantitative) topological diagram or flavor SU(3) approach and the (quantitative but somewhat model-dependent) QCD factorization (QCDF) approach in these decays, by explicitly showing how to translate each flavor SU(3) amplitude into the corresponding terms in the QCDF framework. After estimating each flavor SU(3) amplitude numerically using QCDF, we discuss various physical consequences, including SU(3) breaking effects and some useful SU(3) relations among decay amplitudes of $\bar B_s \to PV$ and $\bar B_d \to PV$.Comment: 47 pages, 3 figures, 28 table

Aspects of four-jet production in polarized proton-proton collisions

We examine the intrinsic spin-dependence of the dominant $gg \rightarrow gggg$ subprocess contribution to four-jet production in polarized proton-proton collisions using helicity amplitude techniques. We find that the partonic level, longitudinal spin-spin asymmetry, $\hat{a}_{LL}$, is intrinsically large in the kinematic regions probed in experiments detecting four isolated jets. Such events may provide another qualitative or semi-quantitative test of the spin-structure of QCD in planned polarized $pp$ collisions at RHIC.Comment: 9 pages, LaTeX, 2 uuencoded postscript files attache

Association of Traffic-Related Air Pollution with Children’s Neurobehavioral Functions in Quanzhou, China

http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000270874101349&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Public, Environmental &amp; Occupational HealthSCI(E)CPCI-S(ISTP)06S228-S2292

A consistent treatment for pion form factors in space-like and time-like regions

We write down some relevant matrix elements for the scattering and decay processes of the pion by considering a quark-meson vertex function. The pion charge and transition form factors $F_\pi$, $F_{\pi\gamma}$, and $F_{\pi\gamma^*}$ are extracted from these matrix elements using a relativistic quark model on the light-front. We found that, the form factors $F_\pi$ and $F_{\pi\gamma}$ in the space-like region agree well with experiment. Furthermore, the branching ratios of all observed decay modes of the neutral pion, that are related to the form factors $F_{\pi\gamma}$ and $F_{\pi\gamma^*}$ in the time-like region, are all consistent with the data as well. Additionally, $F_\pi$ in the time-like region, which deals with the nonvalence contribution, is also discussed.Comment: 24 pages, 6 figures, to appear in Phys. Rev.