4 research outputs found
Implementaci贸n de un protocolo para la producci贸n de ra铆ces pilosas (hairy roots) de u帽a de gato (Uncaria tomentosa) mediante transformaci贸n con Agrobacterium rhizogenes
The beneficial health proper ties registered of secondary metabolites produced by the plant cat鈥檚 claw (Uncaria tomentosa) had generated a strong market demand and intensive extraction of this species in the countries where distributed, with the deterioration of this genetic resource in its natural habitat. Because of this, is necessary to implement protocols for cell and tissue culture of this species in order to achieve the synthesis of compounds in a controlled manner.The root bark is one of the tissues where the production of these compounds is concentrated. Because of this,the production of hairy roots becomes an alternative technique for scale production of the metabolites of interest. In this research, a protocol was implemented using agroinfection of U. tomentosa shoot tips with wild strains of Agrobacterium rizhogenes (AR1500 and A4RS) and maintenance of hairy roots obtained. In collaboration with the Laboratory of Molecular Biology of the PIPRA program (UC Davis), we determined the efficacy of this agroinfection protocol and the use of other molecular tools which showed satisfactory results in testing agroinfection under the methodologies established.Los beneficios para la salud registrados a partir del uso de metabolitos secundarios de la planta llamada u帽a de gato (Uncaria tomentosa) han generado una fuerte demanda comercial, as铆 como la extracci贸n intensiva de esta especie en los pa铆ses en los cuales se distribuye, con el consecuente deterioro de este recurso gen茅tico en su h谩bitat natural. Es por eso que resulta necesario implementar protocolos de cultivo de c茅lulas y tejidos de esta especie, con el fin de lograr la s铆ntesis de los compuestos en forma controlada. La corteza de las ra铆ces es uno de los tejidos en los que se concentra la producci贸n de estos compuestos, raz贸n por la cual la producci贸n de ra铆ces de cabellera (hairy roots) resulta ser una t茅cnica alternativa para la producci贸n a escala de los metabolitos de inter茅s. En este proyecto se implement贸 un protocolo de agroinfecci贸n de microestacas de U. tomentosa utilizando cepas silvestres de Agrobacterium rizhogenes (AR1500 y A4RS), as铆 como el mantenimiento en medio l铆quido de las ra铆ces pilosas obtenidas. En colaboraci贸n con el Laboratorio de Biolog铆a Molecular del programa PIPRA (UC Davis), se determin贸 la eficacia del protocolo de agroinfecci贸n, as铆 como el uso de otras herramientas moleculares para la detecci贸n de expresi贸n g茅nica, las cuales mostraron resultados satisfactorios en los ensayos de agroinfecci贸n, bajo las metodolog铆as establecidas en el proyecto
Implementaci贸n de un protocolo para la producci贸n de ra铆ces pilosas (hairy roots) de u帽a de gato (Uncaria tomentosa) mediante transformaci贸n con Agrobacterium rhizogenes
The beneficial health proper ties registered of secondary metabolites produced by the plant cat鈥檚 claw (Uncaria tomentosa) had generated a strong market demand and intensive extraction of this species in the countries where distributed, with the deterioration of this genetic resource in its natural habitat. Because of this, is necessary to implement protocols for cell and tissue culture of this species in order to achieve the synthesis of compounds in a controlled manner.The root bark is one of the tissues where the production of these compounds is concentrated. Because of this,the production of hairy roots becomes an alternative technique for scale production of the metabolites of interest. In this research, a protocol was implemented using agroinfection of U. tomentosa shoot tips with wild strains of Agrobacterium rizhogenes (AR1500 and A4RS) and maintenance of hairy roots obtained. In collaboration with the Laboratory of Molecular Biology of the PIPRA program (UC Davis), we determined the efficacy of this agroinfection protocol and the use of other molecular tools which showed satisfactory results in testing agroinfection under the methodologies established.Los beneficios para la salud registrados a partir del uso de metabolitos secundarios de la planta llamada u帽a de gato (Uncaria tomentosa) han generado una fuerte demanda comercial, as铆 como la extracci贸n intensiva de esta especie en los pa铆ses en los cuales se distribuye, con el consecuente deterioro de este recurso gen茅tico en su h谩bitat natural. Es por eso que resulta necesario implementar protocolos de cultivo de c茅lulas y tejidos de esta especie, con el fin de lograr la s铆ntesis de los compuestos en forma controlada. La corteza de las ra铆ces es uno de los tejidos en los que se concentra la producci贸n de estos compuestos, raz贸n por la cual la producci贸n de ra铆ces de cabellera (hairy roots) resulta ser una t茅cnica alternativa para la producci贸n a escala de los metabolitos de inter茅s. En este proyecto se implement贸 un protocolo de agroinfecci贸n de microestacas de U. tomentosa utilizando cepas silvestres de Agrobacterium rizhogenes (AR1500 y A4RS), as铆 como el mantenimiento en medio l铆quido de las ra铆ces pilosas obtenidas. En colaboraci贸n con el Laboratorio de Biolog铆a Molecular del programa PIPRA (UC Davis), se determin贸 la eficacia del protocolo de agroinfecci贸n, as铆 como el uso de otras herramientas moleculares para la detecci贸n de expresi贸n g茅nica, las cuales mostraron resultados satisfactorios en los ensayos de agroinfecci贸n, bajo las metodolog铆as establecidas en el proyecto
Implementaci贸n de un protocolo para la producci贸n de ra铆ces pilosas (hairy roots) de u帽a de gato (Uncaria tomentosa) mediante transformaci贸n con Agrobacterium rhizogenes
The beneficial health proper ties registered of secondary metabolites produced by the plant cat鈥檚 claw (Uncaria tomentosa) had generated a strong market demand and intensive extraction of this species in the countries where distributed, with the deterioration of this genetic resource in its natural habitat. Because of this, is necessary to implement protocols for cell and tissue culture of this species in order to achieve the synthesis of compounds in a controlled manner.The root bark is one of the tissues where the production of these compounds is concentrated. Because of this,the production of hairy roots becomes an alternative technique for scale production of the metabolites of interest. In this research, a protocol was implemented using agroinfection of U. tomentosa shoot tips with wild strains of Agrobacterium rizhogenes (AR1500 and A4RS) and maintenance of hairy roots obtained. In collaboration with the Laboratory of Molecular Biology of the PIPRA program (UC Davis), we determined the efficacy of this agroinfection protocol and the use of other molecular tools which showed satisfactory results in testing agroinfection under the methodologies established.Los beneficios para la salud registrados a partir del uso de metabolitos secundarios de la planta llamada u帽a de gato (Uncaria tomentosa) han generado una fuerte demanda comercial, as铆 como la extracci贸n intensiva de esta especie en los pa铆ses en los cuales se distribuye, con el consecuente deterioro de este recurso gen茅tico en su h谩bitat natural. Es por eso que resulta necesario implementar protocolos de cultivo de c茅lulas y tejidos de esta especie, con el fin de lograr la s铆ntesis de los compuestos en forma controlada. La corteza de las ra铆ces es uno de los tejidos en los que se concentra la producci贸n de estos compuestos, raz贸n por la cual la producci贸n de ra铆ces de cabellera (hairy roots) resulta ser una t茅cnica alternativa para la producci贸n a escala de los metabolitos de inter茅s. En este proyecto se implement贸 un protocolo de agroinfecci贸n de microestacas de U. tomentosa utilizando cepas silvestres de Agrobacterium rizhogenes (AR1500 y A4RS), as铆 como el mantenimiento en medio l铆quido de las ra铆ces pilosas obtenidas. En colaboraci贸n con el Laboratorio de Biolog铆a Molecular del programa PIPRA (UC Davis), se determin贸 la eficacia del protocolo de agroinfecci贸n, as铆 como el uso de otras herramientas moleculares para la detecci贸n de expresi贸n g茅nica, las cuales mostraron resultados satisfactorios en los ensayos de agroinfecci贸n, bajo las metodolog铆as establecidas en el proyecto
Uniform ripening Encodes a Golden 2-like Transcription Factor Regulating Tomato Fruit Chloroplast Development
[EN] Modern tomato (Solanum lycopersicum) varieties are bred for uniform ripening (u) light green fruit phenotypes to facilitate harvests of evenly ripened fruit. U encodes a Golden 2-like (GLK) transcription factor, SlGLK2, which determines chlorophyll accumulation and distribution in developing fruit. In tomato, two GLKs-SlGLK1 and SlGLK2-are expressed in leaves, but only SlGLK2 is expressed in fruit. Expressing GLKs increased the chlorophyll content of fruit, whereas SlGLK2 suppression recapitulated the u mutant phenotype. GLK overexpression enhanced fruit photosynthesis gene expression and chloroplast development, leading to elevated carbohydrates and carotenoids in ripe fruit. SlGLK2 influences photosynthesis in developing fruit, contributing to mature fruit characteristics and suggesting that selection of u inadvertently compromised ripe fruit quality in exchange for desirable production traits.Minimum Information About a Microarray Experiment (MIAME)-compliant microarray data are available at http://ted.bti.cornell.edu. and at http://www.ebi.ac.uk/arrayexpress/(accession E-MEXP-3652). F. Carrari and A. Fernie provided S. pennellii SlGLK2, and J. Maloof provided S. habrochaites SlGLK2 sequences. The U. S. Department of Agriculture (USDA)/National Institute of Food and Agriculture Solanaceae Coordinated Agricultural Project provided potato data. We are grateful to the Tomato Genome Consortium and the SOL Genomics Network for prepublication access to the tomato genome sequence. The S. pennellii introgression lines were provided by the C. M. Rick Tomato Genetics Resource Center; the S. pimpinellifolium populations were provided by the Instituto de Hortofruticultura Subtropical y Mediterranea "La Mayora," Consejo Superior de Investigaciones Cientificas; and both populations are available by request from the sources. The AtGLK-expressing lines were provided by Mendel Biotechnology and Seminis/Monsanto Vegetable Seeds. SlGLK2, the corresponding lines, and the F2 10-1 IL x M82 population lines and seeds are available from J. J. G. without restriction. Seminis/Monsanto will make available, upon request, and under a material transfer agreement indicating they are to be used for noncommercial purposes, the following lines: LexA:AtGLK1:p35S:LexA-Gal4; LexA:AtGLK1:pLTP:LexA-Gal4; LexA:AtGLK1:pRbcS:LexA-Gal4; LexA:AtGLK1:pPDS:LexA-Gal4; LexA:AtGLK2:p35S:LexA-Gal4; LexA:AtGLK2:pLTP:LexA-Gal4; LexA:AtGLK2:pRbcS:LexA-Gal4; LexA:AtGLK2:pPDS:LexA-Gal4; plus the T63 control line. Other biological materials are available by request from A. L. T. P. or J.J.G. A. L. T. P., T. H., K.L.-C., R.F.-B., and A. B. B. have filed a provisional U. S. patent application UC #2011-841, "Introduction of wild species GLK genes for improved ripe tomato fruit quality," through the University of California. A. L. T. P. and A. B. B. have filed the U. S. patent application #2010/0154078, " Transcription factors that enhance traits in plant organs," through Mendel Biotechnology. Assistance from B. Blanco-Ulate, S. Phothiset, S. Reyes, A. Abraham, L. Gilani, and G. Arellano is gratefully acknowledged. J. Langdale provided helpful advice regarding GLK phylogeny and nomenclature. G. Adamson and P. Kysar, Electron Microscopy (EM) Laboratory, University of California Davis Medical Center did the EM work. University of California Discovery and partners funded the pepper analysis and the initial investigations of the Arabidopsis GLKs. The Vietnam Education Foundation supported C.N. Fundacion Genoma Espana ESPSOL Project provided partial funding to A.G. USDA-Agricultural Research Service, USDA-National Research Initiative (2007-02773), and NSF (Plant Genome Program IOS-0923312) provided support to J.J.G.Powell, AL.; Nguyen, CV.; Hill, T.; Cheng, KL.; Figueroa-Balderas, R.; Aktas, H.; Ashrafi, H.... (2012). Uniform ripening Encodes a Golden 2-like Transcription Factor Regulating Tomato Fruit Chloroplast Development. Science. 336:1711-1715. doi:10.1126/science.1222218S1711171533