A faunistic study on the braconid wasps (Hymenoptera: Braconidae) of Iran

Twenty-three species of Braconidae (Hymenoptera) within eight subfamilies, Agathidinae, Alysiinae, Blacinae, Brachistinae, Braconinae, Cheloninae, Euphorinae, Macrocentrinae, Microgastrinae and Neoneurinae are recorded for the fauna of Iran. Five species, Macrocentrus grandii Goidanich, 1937 (Macrocentrinae), Apanteles elpis Nixon, 1973, Apanteles urgo Nixon, 1965, Microplitis sofron Nixon, 1970, and Protapanteles aliphera (Nixon, 1973) (Microgastrinae) are newly recorded from Iran

A faunistic study on Chalcidoidea (Hymenoptera) of Iran

Abstract: The fauna of some families of Iranian Chalcidoidea is studied. In total, 24 species of six families, Chalcididae (3 species from 3 genera Brachymeria Westwood, Chalcis Fabricius and Dirhinus Dalman), Encyrtidae (7 species from 6 genera Anagyrus Howard, 1896, Copidosoma Ratzeburg, 1844, Diversinervus Silvestri, 1915, Encyrtus Latreille, 1809, Microterys Thomson, 1876 and Syrphophagus Ashmead, 1900), Eupelmidae (8 species from 4 genera Anastatus Motschulsky, Eupelmus Dalman, Eusandalum Ratzeburg and Pentacladia Westwood), Eurytomidae (3 species from 2 genera Eurytoma Illiger and Tetramesa Walker), Mymaridae (single species from the genus Anaphes Haliday) and Perilampidae (2 species from the genus Perilampus Latreille) were collected and identified

Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch.) Sorokin (Ascomycota: Hypocreales) against two Species of Storage Pest

The susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch.) Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, five aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v). Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and significant difference was observed between concentrations. The corrected cumulative mortality of adult of O. surinamensis 10 days after immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. These amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for different concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. The parameters of probit analysis demonstrated non-overlap of 95% confidence limits of LC50 and LC95 and significant difference was observed among three isolates tested against each insect. The lowest and the highest LC50 and LC95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×105 and 1/5×108) and IRAN 715C for T. castaneum (6.2×108 and 6.9×1014), respectively. This observation highlights the importance and need of screening for more virulent isolates against storage pests for use in the management of these pests

Laboratory Bioassay of Iranian Isolates of Entomopathogenic Fungus Metarhizium anisopliae (Metsch.) Sorokin (Ascomycota: Hypocreales) against two Species of Storage Pest 36 Adel KHASHAVEH, Hamid Sakenin CHELAV

Summary Th e susceptibility of adults of Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) to three Iranian Isolates of the entomopathogenic fungi Metarhizium anisopliae (Metsch.) Sorokin was evaluated through bioassays with direct immersion under laboratory conditions. For each isolates, fi ve aqueous suspensions were prepared in a logarithmic series in Tween 80 (0.05% v/v). Results showed that adult of both species were susceptible to all isolates of M. anisopliae. For all three isolates, mortality percentage of the two species increased with increasing conidial concentration and signifi cant diff erence was observed between concentrations. Th e corrected cumulative mortality of adult of O. surinamensis 10 days aft er immersion ranged from 12.38 to 85.84%, 18.6 to 62.83% and 10.63 to 77.87% for diff erent concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. Th ese amounts for T. castaneum varied from 31.07 to 74.78%, 26.02 to 75.61% and 23.33 to 89.99% for diff erent concentrations of DEMI001, IRAN 715C and IRAN 1018C, respectively. Th e parameters of probit analysis demonstrated non-overlap of 95% confi dence limits of LC 50 and LC 95 and signifi cant diff erence was observed among three isolates tested against each insect. Th e lowest and the highest LC 50 and LC 95 values were observed in the isolates DEMI001 for O. surinamensis (3/1×10 5 and 1/5×10 8) and IRAN 715C for T. castaneum (6.2×10 8 and 6.9×10 14 ), respectively. Th is observation highlights the importance and need of screening for more virulent isolates against storage pests for use in the management of these pests

Dendroseius

Key to the species of Dendroseius (deutonymph and female) 1 Most of dorsal setae of prodorsum and opisthonotum short and equal in length, I 4 1 / 3 distance I 4 to I 5, Z 4 1 / 2 distance Z 4 to Z 5, only shoulder setae r 5 and caudal setae Z 5 longer, female 270–290 µm long ................. D. reticulatus (Sheals, 1956) (Palaearctic) ­ Most of dorsal setae of opisthonotum longer than setae of prodorsum, I 4 = 1 / 2 to 3 / 4 dis­ tance I 4 to I 5, Z 4 reaches the base of Z 5......................................................................... 2 2 Only the dorsal setae of the posterior half of opisthonotum longer than the other dorsal setae, I 4 about 2 x I 1, Z 5 = 3 x I 5, prongs of tectum distally serrated............................... 3 ­ All dorsal setae of opisthonotum longer than the other dorsal setae, I 4 and I 1 about the same length, Z 5 = 2 x I 5, prongs of tectum distally pointed and smooth....................... 4 3 Caudal setae Z 5 2 x the length of i 4, I 5 half the length of i 4, I 1 about 1 / 3 distance I 1 to I 2, digitus fixus of chelicerae only with 3 distal teeth, deutonymph 255–295 µm long...... .............................................. D. congoensis Wiśniewski et Hirschmann, 1992 (Africa) ­ Caudal setae Z 5 3 x the length of i 4, I 5 and i 4 in equal length, I 1 about 1 / 2 distance I 1 to I 2, digitus fixus of chelicerae with 5 distal teeth, deutonymph 325 µm long...................... ..................................................... D. gujarati Wiśniewski et Hirschmann, 1989 (India) 4 Ventrianal shield of female nearly triangular and with 5 pairs of setae, peritreme anteriorly reaches to the level of middle of coxa III, dorsal shields reticulated over entire surface, middle prong of tectum shorter than lateral prongs, female 367 µm long........ ............................................................................................... D. amoliensis n. sp. (Iran) ­ Ventrianal shield of female nearly quadratic and with 6 pairs of setae, peritreme reaches anteriorly to the level of anterior margin of coxa II, only posterior portion of posterior dorsal shield reticulated, prongs of tectum in equal length, female 364 µm long ............................................................ D. badenhorsti (Ryke, 1962) (South Africa)Published as part of Faraji, Farid, Sakenin-Chelav, Hamid & Karg, Wolfgang, 2006, A new species of Dendroseius Karg from Iran (Acari: Rhodacaridae), with a key to the known species, pp. 63-68 in Zootaxa 1221 on pages 66-67, DOI: 10.5281/zenodo.17255

Dendroseius amoliensis Faraji, Sakenin-Chelav & Karg, 2006, n. sp.

Dendroseius amoliensis n. sp. Figs. 1–8 Dendroseius amoliensis resembles D. badenhorsti (Ryke, 1962) closely. The new species is distinguished from the other in having 5 pairs of setae on ventrianal shield instead of 6, ventrianal shield roughly triangular instead of quadratic, peritreme anteriorly reaches to the level of mid­coxa III instead of reaching to the level of anterior margin of coxa II, peritreme situated on a slightly wide peritremal plate instead of a narrow peritremal plate, shorter middle prong of tectum and reticulated over entire dorsal shield. Female (n= 5). Dorsal idiosoma. (Fig. 1) — Dorsal shield divided 350 (357) long, 173 (170) wide at I 1; reticulate over entire surface; bears 36 pairs of dorsal setae, 19 pairs on each anterior and posterior dorsal shield; all dorsal setae simple; lateral membrane with 4 pairs of simple marginal setae of r 5­7 and R 1; length of dorsal setae: i 1 19 (18), i 2 22 (22), i 3 20 (20), i 4 20 (19), i 5 19 (19), I 1 23 (22), I 2 24 (23), I 3 23 (23), I 4 24 (24), I 5 20 (19), z 1 22 (21), z 2 20 (20), z 3 22 (21), z 4 20 (20), z 5 21 (21), Z 1, Z 2 23 (23), Z 3 23 (24), Z 4 28 (28), Z 5 40 (40), s 1 20 (20), s 2 21 (21), s 3, s 4 22 (22), s 5 21 (21), S 1 21 (22), S 2 23 (23), S 3 22 (23), S 4 26 (26), S 5 29 (29), r 1 12 (11), r 2 18 (18), r 3 20 (20), r 4, r 6 18 (18), r 5 28 (27), r 7 19 (19), R 1 15 (16), R 2 21 (21), R 3 20 (20), R 4 20 (21), R 5 22 (23); anterior and posterior dorsal shields with 1 and 5 pairs of visible pores respectively; two pairs of scleronoduli are located between i 4 and i 5; anterior margin of posterior dorsal shield with two pairs of small incisions (this feature is clearly recognizable in the holotype and one of the paratypes). Ventral idiosoma. (Fig. 2) — All ventral setae simple; tritosternum 65 (67) with paired laciniae and pilose with slightly short piles, free for more than half of total length; sternal shield divided, anterior part weakly sclerotized bearing setae ST 1 27 (28), posterior part sclerotized and slightly reticulated with 3 pairs of setae of ST 2 27 (27), ST 3, ST 4 24 (24) and 3 pairs of pores, length of sternal shield (including anterior part) 103 (104), excluding anterior part 75 (75) long at midline, width at the level of ST 2 55 (56) long; epigynial shield truncate posteriorly and slightly reticulated, 55 (55) wide at the level of ST 5, with a pair of simple setae ST 5 21 (22), paragenital pores in soft cuticle posterior to ST 5; slender platelets located between epigynal and ventrianal shields; ventrianal shield roughly triangular and reticulated 112 (116) long and 125 (123) wide, with 5 pairs of setae of JV 1 17 (17), JV 2, JV 3 20 (20), ZV 2 19 (19), para­anal setae 33 (32) and post­anal seta 17 (18); anus 17 (18) long; ZV 1 17 (17), ZV 3 20 (20), JV 4 26 (25) and two pairs of small round platelets on soft cuticle surrounding anal shield; metapodal plate 26 (25) long; ventral shields are also faintly punctate; peritreme short on a s­shaped and slightly wide plate, peritreme extending to the level of mid­coxa III, two pores located posteriorly to peritremal plate on soft cuticle; Fig. 5 shows the gonoporus associated with coxa leg IV. Gnathosoma. Tectum (Fig. 3) with three pointed prongs, middle prong 14 (13) shorter than the lateral prongs 18 (17); hypostome (Fig. 6 and 7) with seven rows, six rows with hypognathal denticles most bearing some tiny denticles (the denticles of first row in holotype is not well recognizable, Fig. 7); capitular setae 20 (20), internal posterior rostral setae 19 (20), external 14 (13), and rostral setae 23 (22), all simple; corniculi horn­like and not divided; chelicera (Fig. 4) with fixed digit 35 long and a row of 5 teeth, movable digit 37 long and with 3 teeth; palp apotele two­tined. Legs. Leg I 335 (323), leg II 250 (253), leg III 228 (222), leg IV 305 (304); setation of legs I­II­III­IV: coxae 2 ­ 2 ­ 2 ­ 1, trochanters 6 ­ 5 ­ 5 ­ 5, femora 13 ­ 10 ­ 6 ­ 6, genua 12 ­ 11 ­ 9 ­ 7, tibiae 12 ­ 10 ­ 8 ­ 7; genua and tibiae leg IV without any macrosetae (Fig. 8). Etymology This species is named after the county “Amol” of its origin. Type material Holotype female, Amol, Iran, soil and debris, 25.viii. 2004, collected by H. Sakenin­ Chelav, deposited in National Museum of Natural History, Leiden, The Netherlands (RMNH). Paratypes: four females (RMNH), similar data as holotype.Published as part of Faraji, Farid, Sakenin-Chelav, Hamid & Karg, Wolfgang, 2006, A new species of Dendroseius Karg from Iran (Acari: Rhodacaridae), with a key to the known species, pp. 63-68 in Zootaxa 1221 on pages 64-66, DOI: 10.5281/zenodo.17255

Фауністичне дослідження родини Braconidae (Hymenoptera: Ichneumonoidea) Ірану

In this research, totally 26 species of Braconidae (Hymenoptera) were collected and identified from some regions of Iran: Agathidinae (one species), Alysiinae (four species, three genera), Brachistinae (one species), Cheloninae (four species, single genus), Dirrhopinae (one species), Doryctinae (two species, two genera), Hormiinae (one species), Microgastrinae (six species, three genera), Opiinae (two species, two genera), Rhysipolinae (one species), Rhyssalinae (one species), and Rogadinae (two species, single genus). Five species, Apanteles kubensis Abdinbekova, 1969 (Microgastrinae), Dirrhope rufa Förster, 1851 (Dirrhopinae), Heterospilus (Heterospilus) austriacus (Szépligeti, 1906) (Doryctinae), Microchelonus (Microchelonus) luzhetzkji (Tobias, 1966) (Cheloninae), and Opiostomus (Jucundopius) impatientis (Fischer, 1957) (Opiinae) are new records for the fauna of Iran.В роботі представлені результати дослідження фауни Braconidae (Hymenoptera) деяких регіонів Ірану. Матеріал був зібраний з використанням пасток Малеза, а також за допомогою косіння ентомологічним сачком. Певна кількість матеріалу була опрацьована з ентомологічних колекцій місцевих університетів та природничих музеїв. Загалом зібрано та ідентифіковано 26 видів Braconidae: Agathidinae (1 вид), Alysiinae (4 види, 3 роди), Brachistinae (1 вид), Cheloninae (4 види, 1 рід), Dirrhopinae (1 вид), Doryctinae (2 види, 2 роди), Hormiinae (1 вид), Microgastrinae (6 видів, 3 роди), Opiinae (2 види, 2 роди), Rhysipolinae (1 вид), Rhyssalinae (1 вид) та Rogadinae (2 види, 1 рід). П’ять видів, а саме – Apanteles kubensis Abdinbekova, 1969 (Microgastrinae), Dirrhope rufa Förster, 1851 (Dirrhopinae), Heterospilus (Heterospilus) austriacus (Szépligeti, 1906) (Doryctinae), Microchelonus (Microchelonus) luzhetzkji (Tobias, 1966) (Cheloninae) і Opiostomus (Jucundopius) impatientis (Fischer, 1957) (Opiinae) – є новими знахідками для фауни Ірану. Результати цього дослідження вказують на те, що фауна іранських родини Braconidae Ірану є дуже різноманітною і в той же час маловідомою для широкого кола дослідників. Фауністичні дослідження іранських браконід проводились не в усіх районах. залишається кілька регіонів, де не проводився систематичний, регулярний збір матеріалу, особливо це стосується східної та південної частини країни. Слід відмітити, що представники родини мають певне практичне значення для використання в біологічному методі контролю деяких видів сільськогосподарських та лісових шкідників. Охорона популяцій цих видів-паразитів має неабияке практичне значення в контексті зменшення чисельності популяцій комах-шкідників