SEVERE WHIPWORM INFESTATION - A RARE CAUSE of INFLAMMATORY DYSENTERIC BOWEL-DISEASE

ESCOLA PAULISTA MED,DEPT MED,DISCIPLINA GASTROENTEROL CLIN,RUA BOTUCATU 740,BR-04023 São Paulo,BRAZILESCOLA PAULISTA MED,DEPT MED,DISCIPLINA GASTROENTEROL CLIN,RUA BOTUCATU 740,BR-04023 São Paulo,BRAZILWeb of Scienc

Prevalence of Helicobacter pylori detected by polymerase chain reaction in the oral cavity of periodontitis patients

Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. the oral cavity has been indicated as a possible H. pylori reservoir, and may therefore be involved in the reinfection of the stomach which sometimes follows treatment of H. pylori infection. the objective of the present study was to evaluate the prevalence of H. pylori as detected by polymerase chain reaction (PCR) in the oral cavity of periodontitis patients testing positive for this bacterium in the stomach. Thirty adult patients with alterations of the superior digestive tract, testing urease positive after endoscopy and biopsy, were selected. A full-mouth periodontal examination was performed in every patient and the subjects were allocated to two groups: gingivitis (15 patients) and chronic periodontitis (15 patients). Plaque and saliva samples collected from each patient were stored in 0.5 ml of TE buffer. DNA was extracted from the samples by the boiling method and was evaluated for the presence of H. pylori using the PCR method. JW 22/23 primers were used. the DNA of ATCC H. pylori 43629 (positive control) and water (negative control) were used for controlling the reactions. of the 30 evaluated patients, 13 (43.3%) harbored H. pylori in the mouth. the bacterium was not found on the dorsum of the tongue of any patient, but was found in saliva in three patients (10%), in the supragingival plaque in six patients (20%), and in the subgingival plaque in eight patients (26.6%). the presence of H. pylori was similar in the gingivitis and chronic periodontitis groups. in conclusion, a high percentage of patients harbored H. pylori in their mouth. the bacterium was detected in saliva, supragingival and subgingival plaque, suggesting that these sites may be considered reservoirs for H. pylori in urease-positive patients.Univ São Paulo, Dent Branch, Dept Stomatol, Div Periodont, São Paulo, BrazilUniv São Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Div Gastroenterol, São Paulo, BrazilUniversidade Federal de São Paulo, Div Gastroenterol, São Paulo, BrazilWeb of Scienc

Prevalence of Helicobacter pylori detected by polymerase chain reaction in the oral cavity of periodontitis patients

Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. the oral cavity has been indicated as a possible H. pylori reservoir, and may therefore be involved in the reinfection of the stomach which sometimes follows treatment of H. pylori infection. the objective of the present study was to evaluate the prevalence of H. pylori as detected by polymerase chain reaction (PCR) in the oral cavity of periodontitis patients testing positive for this bacterium in the stomach. Thirty adult patients with alterations of the superior digestive tract, testing urease positive after endoscopy and biopsy, were selected. A full-mouth periodontal examination was performed in every patient and the subjects were allocated to two groups: gingivitis (15 patients) and chronic periodontitis (15 patients). Plaque and saliva samples collected from each patient were stored in 0.5 ml of TE buffer. DNA was extracted from the samples by the boiling method and was evaluated for the presence of H. pylori using the PCR method. JW 22/23 primers were used. the DNA of ATCC H. pylori 43629 (positive control) and water (negative control) were used for controlling the reactions. of the 30 evaluated patients, 13 (43.3%) harbored H. pylori in the mouth. the bacterium was not found on the dorsum of the tongue of any patient, but was found in saliva in three patients (10%), in the supragingival plaque in six patients (20%), and in the subgingival plaque in eight patients (26.6%). the presence of H. pylori was similar in the gingivitis and chronic periodontitis groups. in conclusion, a high percentage of patients harbored H. pylori in their mouth. the bacterium was detected in saliva, supragingival and subgingival plaque, suggesting that these sites may be considered reservoirs for H. pylori in urease-positive patients.Univ São Paulo, Dent Branch, Dept Stomatol, Div Periodont, São Paulo, BrazilUniv São Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Div Gastroenterol, São Paulo, BrazilUniversidade Federal de São Paulo, Div Gastroenterol, São Paulo, BrazilWeb of Scienc

Teníase: uma causa rara de apendicite aguda

Acute appendicitis is the most common surgical condition of acute abdomen. Approximately 7 percent of the population will have appendicitis during their lifetime, with the peak incidence occurring between 10 through 30 years-old. Obstruction of the appendix lumen with subsequent bacterial infection initiates the pathophysiological sequence of acute appendicitis. Obstruction may have multiple causes, including fecalith, lymphoid hyperplasia (related to viral illnesses, including upper respiratory infection, mononucleosis, and gastroenteritis), foreign bodies, carcinoid tumor, and parasites. In Asia, Africa and Latin America, Enterobius vermicularis has been reported as the main parasite that causes appendix obstruction. Rarely, Taenia sp., has been pointed as a cause of parasitic appendicitis. We reported a 30 years-old patient clinically diagnosed with acute appendicitis. The appendectomy was performed through a McBurney incision. The patient's convalescence was uneventful, and he was discharged from hospital 48 hours after operation. Histological examination of the appendix showed acute appendicitis, and it was found a parasite (Taenia sp.) lying inside of the appendix lumen at a transverse section. He has received 10 mg/Kg weight of praziquantel for taeniasis treatment

Ocorrência de enteroparasitas na população geronte de Nova Olinda do Norte Amazonas, Brasil Occurrence of enteroparasites in the elderly population of Nova Olinda do Norte, Amazonas, Brazil

Foi realizado um estudo para avaliar a prevalência de enteroparasitas em um grupo de idosos ribeirinhos, moradores do Município de Nova Olinda do Norte, Estado do Amazonas Brasil, no período de abril e agosto de 1999. Por meio de um estudo de corte transversal foram analisados 81 exames, através do método de Sedimentação Espontânea (Método de Hoffman et al., 1934). Foi constatada positividade em 72,8% dos idosos, predominando o monoparasitismo (43,2%). Os helmintos foram os mais freqüentes (70,4%), destacando-se: Ascaris lumbricoides (35,2%), Trichuris trichiura (16,0%), Ancylostoma duodenale (9,0%) e Strongyloides stercoralis (9,0%). Dentre os protozoários (29,5%), a ocorrência de Entamoeba coli foi de 18,2%, Giárdia lamblia de 7,0% e Entamoeba histolytica 4,5%. Não houve associação estatisticamente significativa entre sexo e grau de parasitismo e entre faixas etárias e condição parasitária. Estes resultados evidenciam um quadro de alta prevalência de parasitas intestinais nesta população e discordam dos reportados por outros pesquisadores quando afirmam que a intensidade da infestação por parasitas diminui na idade avançada. Os achados anteriores exigem das autoridades governamentais medidas de controle e educação para melhorar a qualidade de vida desses idosos, considerando a grave repercussão que esses parasitas tem no estado nutricional dos gerontes de baixa renda.<br>A study was carried out to determine the prevalence of enteroparasites attacking elderly people living in a riverside city of the Municipal district of Nova Olinda do Norte, Amazonas State Brazil. In this cross sectional study were examined the faecal material from 81 elderly people using the method of spontaneous sedimentation (Hoffman et al., 1934). It was found a positiveness in 72,8% of the elderly people examined, predominating the mono parasitism (43,2%). The helminths were more frequents (70,3%): Ascaris lumbricoides (35,2%), Trichuris trichiura (16,0%), Ancylostoma duodenale (9,0%) and Strongyloides stercoralis (9,0%). Among the protozoans (29,7%) the most frequents were: Entamoeba colí (18,2%), Giardia lamblia (7,0%) and Entamoeba histolytica (4,5%). %. There was not a significant statistically association between sex and parasitism degree and between aging profiles and parasitism condition. The above results showed a high prevalence of intestinal parasites in the elderly people of this small town. These results are in disagreement with those reported by another researchers when pointed out that parasitism intensity decreased with aging. The above situation demands from the govern authorities urgent measured of control and education, considering the enormous problems that the enteroparasites cause on the nutritional status and health condition of elderly people with low socio-economic level