The Effects of Visual Color Stimuli on Zebra Finch Behavior and Stress Response

The Australian Zebra Finch, Taeniopygia guttata, is a common vertebrate model for understanding behavioral, neurological, and physiological changes across the life span. The goal of this study was to determine if color in the environment can act as a stimulus and activate the zebra finch stress response. Zebra finches are diurnal and have color vision. Their plumage coloration is sexually dimorphic and they show behavioral changes to color; females prefer males with redder beaks, and both sexes show individual color preferences for materials in nest building. This experiment was conducted to test whether or not a novel color in the environment can elicit a stress response. A colored poster board was introduced to the adult zebra finches’ habitat, and behavioral changes were measured immediately and then again after twenty four hours. In addition, plasma corticosterone (CORT), the main avian stress hormone, concentrations were measured twenty four hours after introduction of the color stimulus. The introduction of the color stimuli resulted in immediate behavioral changes in the birds and increased activity was observed with the addition of green, blue, and red stimuli and decreased activity with the addition of yellow. However, after twenty four hours there were no changes in behavior or plasma CORT levels for any of the colors. These findings suggest that zebra finches show varied behavioral responses to novel stimuli based on color differences and that these changes are temporary

Precipitation of PEG/Carboxyl-modified gold nanoparticles with magnesium pyrophosphate : a new platform for real-time monitoring of loop-mediated isothermal amplification

2016-2017 > Academic research: refereed > Publication in refereed journal201804_a bcmaVersion of RecordRGCOthersRGC: 501413Others: PGMS Project IDs: P0009500 and P0009150Publishe

The role of urban agriculture for a resilient city

Humans are simultaneously facing challenges as climate change, epidemics and scarcity of food and water. It is estimated that by 2021 over 690 million of people will face hunger; by 2050 the global population will increase up to 10 billion with 68% of the population living in urban areas. By providing 30% of self-sufficient food in 2030, urban agriculture will be a practical concept to face these challenges. The work studies the role of agricultural land as a critical part for a resilient city. Parameters related to food production are also explored. As study case, this work aims to investigate the current food security of the Ho Chi Minh city (HCMC), offering productive green solutions at different scales from land-use planning, urban design to green roofs. For a production of 6.7 kg/day of vegetables a day, the costs of are approximately $10,000 for nearly 5.6 square meters of land; this points out A-Go-Gro technology as an effective measure for vertical farming. For example, 0.18 ha of green space can produce 2 tons of vegetables per day in the Lake View settlement (district 2 in HCMC). Moreover, due to green roofs, stormwater volumes directed into the sewer system are decreased by 65% and the penetration of electromagnetic radiation is reduced by 99.4%.Loài người đang đồng thời đối mặt với những thách thức như biến đổi khí hậu, dịch bệnh, khan hiếm thực phẩm và nước. Ước tính đến năm 2021 có hơn 690 triệu người đói và đến năm 2050 dân số toàn cầu tăng lên gần 10 tỷ người, với 68% sống ở khu vực thành thị. Được sử dụng để tự cung tự cấp 30% lương thực vào năm 2030, nông nghiệp đô thị là một khái niệm hiệu quả cho những thách thức. Bài báo là nghiên cứu đất nông nghiệp như một phần quan trọng cho một thành phố có khả năng phục hồi. Các thông số liên quan đến sản xuất lương thực được nghiên cứu. Bài báo cũng tìm hiểu an ninh lương thực của thành phố Hồ Chí Minh hiện nay. Hơn nữa, bài báo đưa ra các giải pháp phủ xanh hiệu quả trên các quy mô khác từ quy hoạch sử dụng đất, thiết kế đô thị đến mái nhà xanh tại các hộ gia đình. Với chi phí 10.000 USD và gần 5,6 mét vuông đất trồng 6,7 kg rau mỗi ngày, công nghệ A-Go-Gro là một biện pháp hữu hiệu cho canh tác theo chiều dọc. Như vậy 0,18 ha không gian xanh có thể sản xuất 2 tấn rau mỗi ngày tại khu dân cư Lake View ở quận 2. Hơn nữa, bằng cách làm mái nhà xanh, nước mưa giảm đến 65% vào hệ thống cống và sự xâm nhập bức xạ điện từ giảm 99,4%

LY86-64: Implementation And Evaluation Of A Y86 Browser-Based Simulator

The Y86-64 PIPE project is a seminal project for Appalachian State University Computer Science majors. For many students, it is their first software product of significant size. After completing the project, students have a better understanding of computer systems and the software engineering skills and tools needed to develop large pieces of software. However, to implement the code, students need a sophisticated understanding of the machine being simulated. Of particular difficulty is the control logic and signals that direct the stages of the PIPE machine. Several Y86-64 simulators are available, but existing simulators display only the contents of memory and general-purpose registers. They do not provide a visualization of the complicated control logic and signals applied to pipeline registers. For this reason, we undertook the development of the LY86-64 (pronounced “lee 86-64”), a Y86-64 browser-based simulator. A survey of 47 students, some currently studying the Y86-64 PIPE machine and some who studied the machine in a previous semester, found that 100% of respondents believed that the simulator provides a better understanding of the control logic

CONTAMINATION BY PERSISTENT ORGANIC POLLUTANTS AND ENDOCRINE DISRUPTING CHEMICALS IN VIETNAM : PATTERNS, BEHAVIOR, TRENDS AND TOXIC POTENTIAL

Joint Research on Environmental Science and Technology for the Eart

Platinum nanoparticles on reduced graphene oxide as peroxidase mimetics for the colorimetric detection of specific DNA sequence

2015-2016 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

PM2.5 Exposure Suppresses Dendritic Maturation in Subgranular Zone in Aged Rats

published_or_final_versio

A minimal CRISPR-Cas3 system for genome engineering [preprint]

CRISPR-Cas technologies have provided programmable gene editing tools that have revolutionized research. The leading CRISPR-Cas9 and Cas12a enzymes are ideal for programmed genetic manipulation, however, they are limited for genome-scale interventions. Here, we utilized a Cas3-based system featuring a processive nuclease, expressed endogenously or heterologously, for genome engineering purposes. Using an optimized and minimal CRISPR-Cas3 system (Type I-C) programmed with a single crRNA, large deletions ranging from 7 - 424 kb were generated in Pseudomonas aeruginosa with high efficiency and speed. By comparison, Cas9 yielded small deletions and point mutations. Cas3-generated deletion boundaries were variable in the absence of a homology-directed repair (HDR) template, and successfully and efficiently specified when present. The minimal Cas3 system is also portable; large deletions were induced with high efficiency in Pseudomonas syringae and Escherichia coli using an “all-in-one” vector. Notably, Cas3 generated bi-directional deletions originating from the programmed cut site, which was exploited to iteratively reduce a P. aeruginosa genome by 837 kb (13.5%) using 10 distinct crRNAs. We also demonstrate the utility of endogenous Cas3 systems (Type I-C and I-F) and develop an “anti-anti-CRISPR” strategy to circumvent endogenous CRISPR-Cas inhibitor proteins. CRISPR-Cas3 could facilitate rapid strain manipulation for synthetic biological and metabolic engineering purposes, genome minimization, and the analysis of large regions of unknown function

Bioefficacy of leaf extracts from Pouzolzia zeylanica (L.) Benn against diamondback moth plutella xylostella in Viet Nam

In Viet Nam, Pouzolzia zeylanica (L.) Benn is a native plant and has been demonstrated its applicability as a medical plant. Additionally, Pouzolzia zeylanica was used to control fly larvae during food processing due to insecticidal activity. We optimized the extraction of Pouzolzia zeylanica by ethanol at different conditions: concentration, the ratio of solid (material) - liquid (ethanol volume) (mg/ml) and the extraction time (hour). Results indicated that extraction yield was effected by all of the factors. The optimized extraction yield was 6.85% (Y) with ethanol concentration at 96 percent ethanol (Z1), the ratio solid to liquid is 1: 25 (mg/ml) (Z2) and extraction time is 4 days (Z3). We tested the efficiency of leaf extracts from Pouzolzia zeylanica and antifeedant activity against diamondback moth Plutella xylostella at different leaf extract concentrations. Results indicated that 80% mortality induced by those compounds was recorded on Plutella xylostella second instars at 30% leaf extract concentration and had significant difference compared to the control (P=0.0000); the leaf extract affected the ratio of pupation, adult emergence and antifeedant activity of P. xylostella (P=0.0000). The obtained results promise a potential of using Pouzolzia zeylanica as biopesticide in Viet Nam