Acidic Osteoid Templates the Plywood Structure of Bone Tissue

Bone is created by osteoblasts that secrete osteoid after which an ordered texture emerges, followed by mineralization. Plywood geometries are a hallmark of many trabecular and cortical bones, yet the origin of this texturing in vivo has never been shown. Nevertheless, extensive in vitro work revealed how plywood textures of fibrils can emerge from acidic molecular cholesteric collagen mesophases. This study demonstrates in sheep, which is the preferred model for skeletal orthopaedic research, that the deeper non fibrillar osteoid is organized in a liquid crystal cholesteric geometry. This basophilic domain, rich in acidic glycosaminoglycans, exhibits low pH which presumably fosters mesoscale collagen molecule ordering in vivo. The results suggest that the collagen fibril motif of twisted plywood matures slowly through self assembly thermodynamically driven processes as proposed by the Bouligand theory of biological analogues of liquid crystals. Understanding the steps of collagen patterning in osteoid maturation processes may shed new light on bone pathologies that emerge from collagen physico chemical maturation imbalance

Lagrangian measurements of the fast evaporation of falling diethyl ether droplets using in-line digital holography and a high-speed camera

International audienceThe evaporation of falling diethyl ether droplets is measured by following droplets along their trajectories. Measurements are performed at ambient temperature and pressure by using in-line digital holography. The holograms of droplets are recorded with a single high-speed camera and reconstructed with an ''inverse problems'' approach algorithm previously tested (Chareyron et al. New J Phys 14:43039, 2012). Once evaporation starts, the interfaces of the droplets are surrounded by air/vapor mixtures with refractive index gradients that modify the holograms. The central part of the droplets holograms is unusually bright compared to what is expected and observed for non-evaporating droplets. The reconstruction process is accordingly adapted to measure the droplets diameter along their trajectory. The diethyl ether being volatile, the droplets are found to evaporate in a very short time: of the order of 70 ms for a 50-60 lm diameter at an ambient temperature of 25 C. After this time, the diethyl ether has fully evaporated and droplets diameter reaches a plateau. The remaining droplets are then only composed of water, originating from the cooling and condensation of the humid air at the droplet surface. This assertion is supported by two pieces of evidence: (i) by estimating the evolution of droplets refractive index from light scattering measurements at rainbow angle and (ii) by comparing the evaporation rate and droplets velocities obtained by digital holography with those calculated with a simple model of evaporation/condensation. The overall results show that the in-line digital holography with ''inverse problems''approach is an accurate technique for studying fast evaporation from a Lagrangian point of view

Recent Developments in Particle Tracking Diagnostics for Turbulence Research

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