Rapid therapy evaluation using chronic, wide-field optical imaging of microvascular blood flow dynamics

We employ speckle imaging and window chamber models to study vascular remodeling processes after light-based therapies. Our data demonstrate that the response involves substantial remodeling over a monitoring period of up to 30 days post-intervention. © 2007 Optical Society of America

Chronic, wide-field optical imaging of blood flow dynamics

Noninvasive blood flow characterization is essential to assess the health status of biological tissue and to evaluate the efficacy of therapies which target the microvasculature. Optimization of laser therapy for disfiguring vascular birthmarks is one specific clinical application. Current treatment protocols involve the use of high-power pulsed laser irradiation with parameters selected to induce selective photocoagulation of the targeted blood vessels. Protocol design is based largely on results from numerical modeling studies, which have predictive capability of the laser light distribution within the skin and subsequent photothermal response leading towards selective photocoagulation. However, the biological response of the microvasculature to therapeutic laser intervention remains a poorly-researched field. We hypothesize that the acute photothermal response of the microvasculature is a poor predictor of the chronic response, due to vascular remodeling processes which are not included in current modeling studies. To test this hypothesis, we employ an optical imaging method to assess blood flow dynamics in response to therapeutic intervention