Piglets Comfort with Hot Water by Biogas Combustion under Controllable Ventilation

The purpose of this study is to develop a hot-water heating system for pig farms which use biogas as the energy source while the air quality is regulated using an inverter-controlled fan. The biogas is a by-product from the 3-stage wastewater treatment process in regular pig farms. The biogas is burned for hot water which is circulated to warm piglet compartments with regulated, forced ventilation. The hot water is connected to a heat exchanger and hot air is hence blown into the pigsty. To maintain the pigsty at a comfort atmosphere, ventilation is regulated using an inverter-controlled fan. The mechanical ventilation is to be optimized as a compromise between indoor air quality and ventilation rate. The temperature uniformity and air quality in the pigsty is to be secured for comfortability. Experimental results show that hot water circulating at 0.043 m3/min and 60°C could keep the pigsty at 28°C for a stocking density of 1.77 pig/m2. Forced ventilation of 1.7 ACH (air change rate per hour) at 28°C could keep the pigsty comfort in terms of indoor temperature, relative humidity, and carbon-dioxide concentration

On the Wind Reduction Effect of Windbreak Nets in Front of the Simple Greenhouse for Planting Fruits and Vegetables

The island of Taiwan experiences frequent strong winds coming with typhoons in the summer and with northeast monsoons in the winter. The strong winds are hazardous to the structure of a simple greenhouse that is widely used for vegetable and fruit plantation. The damage may reduce agricultural yields. Windbreak netting is a practical procedure for protecting a greenhouse from strong winds. The purpose of this study is to carry on the wind tunnel simulation test of windbreak nets in front of the simple greenhouse. Windbreak nets of densities 50%, 40%, 30% and 20% had a uniform dimension of 10 cm (per unit 1 h) in height and 29.2 cm in width. A windbreak net was tested at tilt angles of 0o, 5o, 10o and 15o. The wind tunnel simulation test is carried on at 10 m/s of reference wind velocity. The wind velocities at different horizontal distances and vertical distances front and behind the windbreak net are measured. The relative wind velocity is calculated. The wind reduction effect at different horizontal distances behind the windbreak net is analyzed and discussed. From the test results, it is known that when the tilt angle of windbreak net is 0o and the height of windbreak net is 1h, the corresponding wind reduction effect of windbreak nets with 20%, 30%, 40% and 50% porosity is 62%, 61%, 59% and 52% respectively at 2h horizontal distance. The corresponding wind reduction effect of windbreak nets with 20%, 30%, 40% and 50% porosity is 46%, 45%, 40% and 32% respectively at 10h horizontal distance. However, the increase of tilt angle of windbreak net does not have much influence on the wind reduction effect

Causal Evidence for the Role of Specific GABAergic Interneuron Types in Entorhinal Recruitment of Dentate Granule Cells

The dentate gyrus (DG) is the primary gate of the hippocampus and controls information flow from the cortex to the hippocampus proper. To maintain normal function, granule cells (GCs), the principal neurons in the DG, receive fine- tuned inhibition from local-circuit GABAergic inhibitory interneurons (INs). Abnormalities of GABAergic circuits in the DG are associated with several brain disorders, including epilepsy, autism, schizophrenia, and Alzheimer disease. Therefore, understanding the network mechanisms of inhibitory control of GCs is of functional and pathophysiological importance. GABAergic inhibitory INs are heterogeneous, but it is unclear how individual subtypes contribute to GC activity. Using cell-type-specific optogenetic perturbation, we investigated whether and how two major IN populations defined by parvalbumin (PV) and somatostatin (SST) expression, regulate GC input transformations. We showed that PV-expressing (PV+) INs, and not SST- expressing (SST+) INs, primarily suppress GC responses to single cortical stimulation. In addition, these two IN classes differentially regulate GC responses to θ and γ frequency inputs from the cortex. Notably, PV+ INs specifically control the onset of the spike series, whereas SST+ INs preferentially regulate the later spikes in the series. Together, PV+ and SST+ GABAergic INs engage differentially in GC input-output transformations in response to various activity patterns

Rikitake dynamo system, its circuit simulation and chaotic synchronization via quasi-sliding mode control

Rikitake dynamo system (1958) is a famous two-disk dynamo model that is capable of executing nonlinear chaotic oscillations similar to the chaotic oscillations as revealed by palaeomagnetic study. First, we detail the Rikitake dynamo system, its signal plots and important dynamic properties. Then a circuit design using Multisim is carried out for the Rikitake dynamo system. New synchronous quasi-sliding mode control (QSMC) for Rikitake chaotic system is studied in this paper. Furthermore, the selection on switching surface and the existence of QSMC scheme is also designed in this paper. The efficiency of the QSMC scheme is illustrated with MATLAB plots

A novel smart somatosensory wearable assistive device for older adults’ home rehabilitation during the COVID-19 pandemic

BackgroundDue to the Coronavirus disease 19 (COVID-19) related social distancing measures and health service suspension, physical activity has declined, leading to increased falling risk and disability, and consequently, compromising the older adult health. How to improve the quality of older adult life has become a crucial social issue.ObjectiveIn traditional rehabilitation, manual and repetitive muscle training cannot identify the patient’s rehabilitation effect, and increasing the willingness to use it is not easy. Therefore, based on the usability perspective, this study aims to develop a novel smart somatosensory wearable assistive device (called SSWAD) combined with wireless surface electromyography (sEMG) and exergame software and hardware technology. The older adult can do knee extension, ankle dorsiflexion, and ankle plantar flexion rehabilitation exercises at home. Meanwhile, sEMG values can be digitally recorded to assist physicians (or professionals) in judgment, treatment, or diagnosis.MethodsTo explore whether the novel SSWAD could improve the older adult willingness to use and motivation for home rehabilitation, 25 frail older adult (12 males and 13 females with an average age of 69.3) perform the rehabilitation program with the SSWAD, followed by completing the system usability scale (SUS) questionnaire and the semi-structured interview for the quantitative and qualitative analyses. In addition, we further investigate whether the factor of gender or prior rehabilitation experience would affect the home rehabilitation willingness or not.ResultsAccording to the overall SUS score, the novel SSWAD has good overall usability performance (77.70), meaning that the SSWAD makes older adult feel interested and improves their willingness for continuous rehabilitation at home. In addition, the individual item scores of SUS are shown that female older adult with prior rehabilitation experience perform better in “Learnability” (t = 2.35, p = 0.03) and “Confidence” (t = −3.24, p = 0.01). On the contrary, male older adult without rehabilitation experience are more willing to adopt new technologies (t = −2.73, p = 0.02), and perform better in “Learnability” (t = 2.18, p = 0.04) and “Confidence” (t = −3.75, p < 0.001) with the SSWAD. In addition, the result of the semi-structured interview shows that the operation of the SSWAD is highly flexible, thus reducing older adult burden during the rehabilitation exercise and using them long-term.ConclusionThis novel SSWAD receives consistently positive feedback regardless of the gender or prior rehabilitation experience of elders. The SSWAD could be used as a novel way of home rehabilitation for elders, especially during the COVID-19 pandemic. Older adult can do rehabilitation exercises at home, and physicians could make proper judgments or adjust suitable treatments online according to the sEMG data, which older adult can know their rehabilitation progress at the same time. Most importantly, older adult do not have to go to the hospital every time for rehabilitation, which significantly reduces time and the risk of infection

High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development

<p>Abstract</p> <p>Background</p> <p>Chicken anemia virus (CAV), the causative agent chicken anemia, is the only member of the genus <it>Gyrovirus </it>of the <it>Circoviridae </it>family. CAV is an immune suppressive virus and causes anemia, lymph organ atrophy and immunodeficiency. The production and biochemical characterization of VP1 protein and its use in a subunit vaccine or as part of a diagnostic kit would be useful to CAV infection prevention.</p> <p>Results</p> <p>Significantly increased expression of the recombinant full-length VP1 capsid protein from chicken anemia virus was demonstrated using an <it>E. coli </it>expression system. The VP1 gene was cloned into various different expression vectors and then these were expressed in a number of different <it>E. coli </it>strains. The expression of CAV VP1 in <it>E. coli </it>was significantly increased when VP1 was fused with GST protein rather than a His-tag. By optimizing the various rare amino acid codons within the N-terminus of the VP1 protein, the expression level of the VP1 protein in <it>E. coli </it>BL21(DE3)-pLysS was further increased significantly. The highest protein expression level obtained was 17.5 g/L per liter of bacterial culture after induction with 0.1 mM IPTG for 2 h. After purification by GST affinity chromatography, the purified full-length VP1 protein produced in this way was demonstrated to have good antigenicity and was able to be recognized by CAV-positive chicken serum in an ELISA assay.</p> <p>Conclusions</p> <p>Purified recombinant VP1 protein with the gene's codons optimized in the N-terminal region has potential as chimeric protein that, when expressed in <it>E. coli</it>, may be useful in the future for the development of subunit vaccines and diagnostic tests.</p

Women with endometriosis have higher comorbidities: Analysis of domestic data in Taiwan

AbstractEndometriosis, defined by the presence of viable extrauterine endometrial glands and stroma, can grow or bleed cyclically, and possesses characteristics including a destructive, invasive, and metastatic nature. Since endometriosis may result in pelvic inflammation, adhesion, chronic pain, and infertility, and can progress to biologically malignant tumors, it is a long-term major health issue in women of reproductive age. In this review, we analyze the Taiwan domestic research addressing associations between endometriosis and other diseases. Concerning malignant tumors, we identified four studies on the links between endometriosis and ovarian cancer, one on breast cancer, two on endometrial cancer, one on colorectal cancer, and one on other malignancies, as well as one on associations between endometriosis and irritable bowel syndrome, one on links with migraine headache, three on links with pelvic inflammatory diseases, four on links with infertility, four on links with obesity, four on links with chronic liver disease, four on links with rheumatoid arthritis, four on links with chronic renal disease, five on links with diabetes mellitus, and five on links with cardiovascular diseases (hypertension, hyperlipidemia, etc.). The data available to date support that women with endometriosis might be at risk of some chronic illnesses and certain malignancies, although we consider the evidence for some comorbidities to be of low quality, for example, the association between colon cancer and adenomyosis/endometriosis. We still believe that the risk of comorbidity might be higher in women with endometriosis than that we supposed before. More research is needed to determine whether women with endometriosis are really at risk of these comorbidities

AI is a viable alternative to high throughput screening: a 318-target study

: High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery