Aphidicidal potential of Azadirachta indica, Adhatoda vasica, Vitex negundo, Parthenium hysterophorus and Lantana camara against mustard aphid, Lipaphis erysimi Kaltenbach (Hemiptera:Aphididae)

Use of eco-friendly naturally occurring plant products in the management of the mustard aphid, Lipaphis erysimi Kaltenbach (Hemiptera:Aphididae) under laboratory conditions was carried out. The results revealed that the nymphs and adults mortality of mustard aphid varied significantly with three different concentrations (0.5, 1.0 and 2.0%) of five plant products. The mortality was high with seed kernel extracts of neem, Azadirachta indica killed nymphs and adults of L. erysimi (70.82 %) followed by leaves extracts of lagundi, Vitex negundo Linn. (69.40 %), leaves extract of Parthenium hysterophorus Linn. (65.38 %), leaves extract of arusa, Adhatoda vasica Nees. (55.81%), aerial parts of aripple, Lantana camara Linn. (51.70%) and untreated control (1.5 %), respectively. The seed kernel extracts of A. indica differed significantly from the remaining ones except V. negundo from which it does not differ significantly to one another. The effects of carrot grass, P. hysterophorus was recorded moderate aphidicidal action while L. camara caused the lowest mortality of nymphs and adults of L. erysimi among all treated natural products. Of the five plant products tested A. indica performed better under all the experimental conditions where as untreated control gave only 01.5% aphid mortality. The concentrations 2.0% are superior to concentration 1.0% and 0.5% concentrations. Similarly maximum aphid mortality was observed after 24 hours of exposure and minimum after 6hours. The exposure periods of 24 hours was significantly superior to 12 hours and 6 hours in both control and plant products

Assessing Sustainable Ecotourism Opportunities in Western Rajasthan, India, through Advanced Geospatial Technologies

The present study focuses on finding potential sites for ecotourism development using GIS and remote-sensing-based weightage sum overlay techniques in Western Rajasthan, India. Ecotourism is one of the fastest growing and revenue-making sectors incorporating a sustainable future. Western Rajasthan has a broad scope to develop tourism-based activity in various ways, mainly through cultural heritage, historical and archaeological wonders, and rare wildlife. Weightage sum overlay analysis is a useful and simple tool to compare each thematic layer. These values are based on various factors and understanding taken during the study. For this purpose, different data types have been taken from the USGS website. Arc GIS 10.8 and ERDAS Imagine software 2015 have been utilized to process the data. This research incorporates seven thematic layers, i.e., elevation, proximity to streams, land use/cover, population density, road connectivity, proximity to protected areas, and heritage hotspots. Based on the physical and cultural characteristics of Western Rajasthan, the weightage of each thematic layer has been decided, which is finally overlaid using Arc GIS software. After processing all the thematic layers, we finally get an outcome in the form of a suitability map. The final suitability map represents five suitability classes that divide the total area into the following categories, very high (37.31%), high (26.85%), moderate (7.89%), low (0.83%), and very low (27.12%), which represents the potential of ecotourism in Western Rajasthan

Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid gestation

© 2020, The Author(s). Transplacental transmission (TPT) of wild-type Indian BTV-1 had never been experimentally proved. This study was first time investigated TPT of Indian BTV-1 (isolated from aborted and stillborn goat fetal spleens). The sequential pathology, virological and immune cell kinetics (CD4+, CD8+ T-lymphocytes and NK cells in spleen and PBMCs), and apoptosis in IFNAR1-blocked pregnant mice during early (infected on 1 GD) and mid (infected on 8 GD) gestation have been studied. There was higher rate of TPT during mid stage (71.43%) than early (57.14%) stage. In early stage reduced implantation sites, early embryonic deaths, abortions, and necro-haemorrhagic lesions had observed. Mid stage, congenital defects and neurological lesions in foetuses like haemorrhages, diffuse cerebral edema, necrotizing encephalitis and decreased bone size (Alizarin red staining) were noticed. BTV-1 antigen was first time demonstrable in cells of mesometrium, decidua of embryos, placenta, uterus, ovary, and brain of foetuses by immunohistochemistry and quantified by real-time qRT-PCR. BTV-inoculated mice were seroconverted by 7 and 5 dpi, and reached peak levels by 15 and 9 dpi in early and mid gestation, respectively. CD4+ and CD8+ cells were significantly decreased (increased ratio) on 7 dpi but subsequently increased on 15 dpi in early gestation. In mid gestation, increased CD8+ cells (decreased ratio) were observed. Apoptotic cells in PBMCs and tissues increased during peak viral load. This first time TPT of wild-type Indian BTV-1 deserves to be reported for implementation of control strategies. This model will be very suitable for further research into mechanisms of TPT, overwintering, and vaccination strategies

Oxidation of Alpha-Ketoglutarate Is Required for Reductive Carboxylation in Cancer Cells with Mitochondrial Defects

SummaryMammalian cells generate citrate by decarboxylating pyruvate in the mitochondria to supply the tricarboxylic acid (TCA) cycle. In contrast, hypoxia and other impairments of mitochondrial function induce an alternative pathway that produces citrate by reductively carboxylating α-ketoglutarate (AKG) via NADPH-dependent isocitrate dehydrogenase (IDH). It is unknown how cells generate reducing equivalents necessary to supply reductive carboxylation in the setting of mitochondrial impairment. Here, we identified shared metabolic features in cells using reductive carboxylation. Paradoxically, reductive carboxylation was accompanied by concomitant AKG oxidation in the TCA cycle. Inhibiting AKG oxidation decreased reducing equivalent availability and suppressed reductive carboxylation. Interrupting transfer of reducing equivalents from NADH to NADPH by nicotinamide nucleotide transhydrogenase increased NADH abundance and decreased NADPH abundance while suppressing reductive carboxylation. The data demonstrate that reductive carboxylation requires bidirectional AKG metabolism along oxidative and reductive pathways, with the oxidative pathway producing reducing equivalents used to operate IDH in reverse

Clinicopathological studies of Pasteurella multocida B:2 experimental infection in rabbits

The present study was carried out to study pathology and virulence of Pasteurella multocida B:2 isolated from natural outbreak of Pasteurella infection in rabbits. Healthy Pasteurella multocida B:2 free rabbits (12) were divided in control and challenged group. The challenged group rabbits were inoculated intra-nasally by spraying of 0.5 ml of inoculum in each nares, containing 1 × 105 CFU of Pasteurella multocida B:2 in BHI. The control group of rabbits was inoculated with 0.5 ml uncultured BHI broth in each nares in the same manner. Live animals were observed for atleast 14 days. The main clinical signs observed in infected rabbits comprised of conjunctivitis, nasal discharge, pyrexia, sneezing, dyspnoea and abdominal breathing. The pathomorphological changes in lungs comprised of acute fibrinous and/or fibrino suppurative bronchopneumonia/pleuropneumonia. Trachea showed acute inflammatory changes with multifocal erosion/ulcer and necrosis. Nasal cavity showed acute inflammation with accumulation of cellular debris in nasal meatus. Three infected rabbits showed meningitis characterized by congestion, infiltration of heterophils and minimal oedema. Pasteurella multocida B:2 was successfully detected in blood and tissues of infected rabbits by PCR. The organisms were also demonstrated in blood/impression smear from different organs

Probing the Role of Magnetic-Field Variations in NOAA AR 8038 in Producing Solar Flare and CME on 12 May 1997

We carried out a multi-wavelength study of a CME and a medium-size 1B/C1.3 flare occurring on 12 May 1997. We present the investigation of magnetic-field variations in the NOAA Active Region 8038 which was observed on the Sun during 7--16 May 1997. Analyses of H{\alpha} filtergrams and MDI/SOHO magnetograms revealed continual but discrete surge activity, and emergence and cancellation of flux in this active region. The movie of these magnetograms revealed two important results that the major opposite polarities of pre-existing region as well as in the emerging flux region (EFR) were approaching towards each other and moving magnetic features (MMF) were ejecting out from the major north polarity at a quasi-periodicity of about ten hrs during 10--13 May 1997. These activities were probably caused by the magnetic reconnection in the lower atmosphere driven by photospheric convergence motions, which were evident in magnetograms. The magnetic field variations such as flux, gradient, and sunspot rotation revealed that free energy was slowly being stored in the corona. The slow low-layer magnetic reconnection may be responsible for this storage and the formation of a sigmoidal core field or a flux rope leading to the eventual eruption. The occurrence of EUV brightenings in the sigmoidal core field prior to the rise of a flux rope suggests that the eruption was triggered by the inner tether-cutting reconnection, but not the external breakout reconnection. An impulsive acceleration revealed from fast separation of the H{\alpha} ribbons of the first 150 seconds suggests the CME accelerated in the inner corona, which is consistent with the temporal profile of the reconnection electric field. In conclusion, we propose a qualitative model in view of framework of a solar eruption involving, mass ejections, filament eruption, CME, and subsequent flare.Comment: 8 figures, accepted for publication in Solar Physic

A hydraulically driven colonoscope

BACKGROUND: Conventional colonoscopy requires a high degree of operator skill and is often painful for the patient. We present a preliminary feasibility study of an alternative approach where a self-propelled colonoscope is hydraulically driven through the colon. METHODS: A hydraulic colonoscope which could be controlled manually or automatically was developed and assessed in a test bed modelled on the anatomy of the human colon. A conventional colonoscope was used by an experienced colonoscopist in the same test bed for comparison. Pressures and forces on the colon were measured during the test. RESULTS: The hydraulic colonoscope was able to successfully advance through the test bed in a comparable time to the conventional colonoscope. The hydraulic colonoscope reduces measured loads on artificial mesenteries, but increases intraluminal pressure compared to the colonoscope. Both manual and automatically controlled modes were able to successfully advance the hydraulic colonoscope through the colon. However, the automatic controller mode required lower pressures than manual control, but took longer to reach the caecum. CONCLUSIONS: The hydraulic colonoscope appears to be a viable device for further development as forces and pressures observed during use are comparable to those used in current clinical practice

Report of the Task Force on Enhancing technology use in agriculture insurance

Pradhan Mantri Fasal Bima Yojana (PMFBY) is a flagship scheme of the Government of India to provide insurance coverage and financial support to farmers in the event of failure of any of the notified crops, unsown area and damage to harvest produce as a result of natural calamities, pests and diseases to stabilise the income of farmers, and to encourage them to adopt modern agricultural practices. The scheme is a considerable improvement over all previous insurance schemes in India and is heavily subsidised by the state and central governments. The scheme aims to cover 50 percent of the farming households within next 3 years. During its implementation in the last one season, several challenges relating to enrolment, yield estimation, loss assessment, and claim settlement were reported by farmers, insurance companies as well as the state governments. It was also noted that several technological opportunities existed for possibly leveraging support to the Indian crop insurance program for enhanced efficiency and effectiveness. NITI Aayog of the Government of India, therefore, constituted a Task Force to deliberate on this subject and identify such potential opportunities. This report summarises the recommendations of the Task Force. The Task Force constituted to address the issue of technology support to crop insurance comprised the following 5 sub-groups: (1) Remote Sensing & Drones; (2) Decision Support Systems, Crop Modelling & Integrated Approaches; (3) IT/ICT in Insurance; (4) Crop Cutting Experiments (CCEs); and (5) Technologies for Livestock and Aquaculture Insurance. Each sub-group had several discussions with experts in the respective areas, and submitted draft reports. More than 100 experts related to professional research agencies, insurance industry, banks, and the government contributed to these discussions. Technological options available in the country and abroad were considered by all groups. The Task Force together with the sub-groups then deliberated on key issues and formulated its recommendations as presented in this report. During the discussions it was realised that there were many administrative and institutional issues that needed to be addressed in PMFBY. However, the focus of the Task Force was on its main mandate, technology use in crop insurance. We hope these recommendations would help the Indian crop insurance sector take full advantage of the technological options suggested so as to increase its efficacy and effectiveness leading to reduced agrarian distress in the country