Measurement of biceps brachii muscle cross-sectional area by extended-field-of-view ultrasound imaging technique

This study investigated the reliability of the extended-field-of-view (EFOV) ultrasonography technique and its validity against magnetic resonance imaging (MRI) for biceps brachii muscle cross-sectional area (CSA) assessment, and applied the method to examine changes in CSA following 10 sets of 3 maximal eccentric contractions of the elbow flexors. Bicep brachii CSA was assessed using both EFOV and MRI techniques at the mid-point of the upper arm. A Pearson product moment analysis showed a high correlation (r = 0.99) between the EFOV and MRI measures; however, the CSA obtained from the EFOV (12.5 ± 6.3 cm² ) was smaller (P=0.004) than that of MRI (12.9 ± 6.1 cm²). The reliability of the EFOV technique was assessed using the same scan image tracing twice (between-traces) and two images scanned from the same site (between-scans), and using the images taken from the same site one hour apart (between-measures). An Intra-class correlation (ICC) analysis showed good reliability (R=1.0) for both between-traces and between-scans, and coefficient of variation (CV) was less than 0.1 %. The reliability was also high for the measurements taken one hour apart (R=0.99, CV=0.7 %). These results show that EFOV is a valid and reliable method to assess biceps brachii CSA, but EFOV could give a smaller (~1 %) CSA than MRI. However, brachial is CSA was difficult to assess in this method, because of the limitation of the scanning technique. Biceps brachii CSA was increased (P\u3c0.05) immediately (8.7 ± 5.8 %) and 4 days (7.7 ± 6.0 %) following eccentric exercise of the elbow flexors, illustrating muscle swelling

The Rotating Mass Matrix, the Strong CP Problem and Higgs Decay

We investigate a recent solution to the strong CP problem, obtaining a theta-angle of order unity, and show that a smooth trajectory of the massive eigenvector of a rank-one rotating mass matrix is consistent with the experimental data for both fermion masses and mixing angles (except for the masses of the lightest quarks). Using this trajectory we study Higgs decay and find suppression of $\Gamma(H\to c\bar{c})$ compared to the standard model predictions for a range of Higgs masses. We also give limits for flavour violating decays, including a relatively large branching ratio for the $\tau^-\mu^+$ mode.Comment: 15 pages, 6 figures; improvements to introduction and preliminarie

Eta model forecasts as input to crop models for the Mid-Southern region of Brazil

Este trabalho tem por objetivo avaliar a precipitação pluvial e as temperaturas máximas e mínimas previstas pelo Modelo Eta para até 120 dias de previsão em 24 localidades distribuídas na região Centro-Sul do Brasil. A avaliação se baseia na comparação de séries históricas de chuva e temperaturas de 1997 a 2002, com as previsões de 30, 60 e 120 dias de antecedência do Modelo Eta para as 24 localidades. Foram utilizados valores de média, mediana e desvio padrão nesta avaliação. Os resultados indicam que estas previsões geralmente subestimam as chuvas e a amplitude térmica nestas localidades. Os menores erros de precipitação pluvial se localizam mais destacadamente em Itumbiara e Rio Verde, enquanto que os maiores, em Porangatu e Manduri. Por outro lado, os menores erros de temperatura máxima se localizam destacadamente em Brasília, Manduri e Piracicaba, enquanto que os maiores ocorrem em Barreiras e Porangatu. Apesar de o modelo apresentar erros sistemáticos nas previsões de temperaturas, estes erros podem ser removidos para que os valores corrigidos possam ser introduzidos nos modelos de culturas.The objective of this study was to evaluate the 120-day precipitation and maximum and minimum temperature forecasts by Eta Model over 24 locations around the Mid-Southern of Brazil. The evaluation was based on comparing observed time series of precipitation and temperatures from 1997 to 2002 to 30, 60 and 120-day forecasts of Eta Model over these 24 locations. Mean, median and standard deviation were used in the evaluation. The results show that these forecasts generally underestimate rain and temperature range. The smallest precipitation errors occurred in Itumbiara and Rio Verde, whereas the largest errors occurred in Porangatu and Manduri. The smallest maximum temperature errors occurred in Brasília, Manduri and Piracicaba whereas the largest errors in Barreiras and Porangatu. Despite the systematic errors exhibited by the precipitation and temperature forecasts, these errors can be removed and the corrected values input into the crop models

Allergen Delivery Inhibitors: Characterisation of Potent and Selective Inhibitors of Der p 1 and Their Attenuation of Airway Responses to House Dust Mite Allergens

Group 1 allergens of house dust mites (HDM) are globally significant triggers of allergic disease. They are considered as initiator allergens because their protease activity enables the development of allergy to a spectrum of unrelated allergens from various sources. This initiator-perpetuator function identifies Group 1 HDM allergens as attractive drug design targets for the first small-molecule approach directed towards a non-human, root cause trigger of allergic disease. The purpose of this study was to: (i) identify exemplar inhibitors of these allergens using Der p 1 as a design template, and (ii) characterise the pharmacological profiles of these compounds using in vitro and in vivo models relevant to allergy. Potent inhibitors representing four different chemotypes and differentiated by mechanism of action were investigated. These compounds prevented the ab initio development of allergy to the full spectrum of HDM allergens and in established allergy they inhibited the recruitment of inflammatory cells and blunted acute allergic bronchoconstriction following aerosol challenge with the full HDM allergen repertoire. Collectively, the data obtained in these experiments demonstrate that the selective pharmacological targeting of Der p 1 achieves an attractive range of benefits against exposure to all HDM allergens, consistent with the initiator-perpetuator function of this allergen

Transcriptional Changes Underlying Elemental Stoichiometry Shifts in a Marine Heterotrophic Bacterium

Marine bacteria drive the biogeochemical processing of oceanic dissolved organic carbon (DOC), a 750-Tg C reservoir that is a critical component of the global C cycle. Catabolism of DOC is thought to be regulated by the biomass composition of heterotrophic bacteria, as cells maintain a C:N:P ratio of ∼50:10:1 during DOC processing. Yet a complicating factor in stoichiometry-based analyses is that bacteria can change the C:N:P ratio of their biomass in response to resource composition. We investigated the physiological mechanisms of resource-driven shifts in biomass stoichiometry in continuous cultures of the marine heterotrophic bacterium Ruegeria pomeroyi (a member of the Roseobacter clade) under four element limitation regimes (C, N, P, and S). Microarray analysis indicated that the bacterium scavenged for alternate sources of the scarce element when cells were C-, N-, or P-limited; reworked the ratios of biomolecules when C- and P- limited; and exerted tighter control over import/export and cytoplasmic pools when N-limited. Under S limitation, a scenario not existing naturally for surface ocean microbes, stress responses dominated transcriptional changes. Resource-driven changes in C:N ratios of up to 2.5-fold and in C:P ratios of up to sixfold were measured in R. pomeroyi biomass. These changes were best explained if the C and P content of the cells was flexible in the face of shifting resources but N content was not, achieved through the net balance of different transcriptional strategies. The cellular-level metabolic trade-offs that govern biomass stoichiometry in R. pomeroyi may have implications for global carbon cycling if extendable to other heterotrophic bacteria. Strong homeostatic responses to N limitation by marine bacteria would intensify competition with autotrophs. Modification of cellular inventories in C- and P-limited heterotrophs would vary the elemental ratio of particulate organic matter sequestered in the deep ocean

Assessment of Hypertension Using Clinical Electrocardiogram Features: A First-Ever Review

Hypertension affects an estimated 1.4 billion people and is a major cause of morbidity and mortality worldwide. Early diagnosis and intervention can potentially decrease cardiovascular events later in life. However, blood pressure (BP) measurements take time and require training for health care professionals. The measurements are also inconvenient for patients to access, numerous daily variables affect BP values, and only a few BP readings can be collected per session. This leads to an unmet need for an accurate, 24-h continuous, and portable BP measurement system. Electrocardiograms (ECGs) have been considered as an alternative way to measure BP and may meet this need. This review summarizes the literature published from January 1, 2010, to January 1, 2020, on the use of only ECG wave morphology to monitor BP or identify hypertension. From 35 articles analyzed (9 of those with no listed comorbidities and confounders), the P wave, QTc intervals and TpTe intervals may be promising for this purpose. Unfortunately, with the limited number of articles and the variety of participant populations, we are unable to make conclusions about the effectiveness of ECG-only BP monitoring. We provide 13 recommendations for future ECG-only BP monitoring studies and highlight the limited findings in pregnant and pediatric populations. With the advent of convenient and portable ECG signal recording in smart devices and wearables such as watches, understanding how to apply ECG-only findings to identify hypertension early is crucial to improving health outcomes worldwide

Allergen Delivery Inhibitors: A Rationale for Targeting Sentinel Innate Immune Signaling of Group 1 House Dust Mite Allergens through Structure-Based Protease Inhibitor Design

Diverse evidence from epidemiologic surveys and investigations into the molecular basis of allergenicity have revealed that a small cadre of “initiator” allergens promote the development of allergic diseases, such as asthma, allergic rhinitis, and atopic dermatitis. Pre-eminent among these initiators are the group 1 allergens from house dust mites (HDM). In mites, group 1 allergens function as cysteine peptidase digestive enzymes to which humans are exposed by inhalation of HDM fecal pellets. Their protease nature confers the ability to activate high gain signaling mechanisms which promote innate immune responses, leading to the persistence of allergic sensitization. An important feature of this process is that the initiator drives responses both to itself and to unrelated allergens lacking these properties through a process of collateral priming. The clinical significance of group 1 HDM allergens in disease, their serodominance as allergens, and their IgE-independent bioactivities in innate immunity make these allergens interesting therapeutic targets in the design of new small-molecule interventions in allergic disease. The attraction of this new approach is that it offers a powerful, root-cause-level intervention from which beneficial effects can be anticipated by interference in a wide range of effector pathways associated with these complex diseases. This review addresses the general background to HDM allergens and the validation of group 1 as putative targets. We then discuss structure-based drug design of the first-in-class representatives of allergen delivery inhibitors aimed at neutralizing the proteolytic effects of HDM group 1 allergens, which are essential to the development and maintenance of allergic diseases