Hepatitis C virus infection protein network

A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein–protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFβ pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins

ViralORFeome: an integrated database to generate a versatile collection of viral ORFs

Large collections of protein-encoding open reading frames (ORFs) established in a versatile recombination-based cloning system have been instrumental to study protein functions in high-throughput assays. Such ‘ORFeome’ resources have been developed for several organisms but in virology, plasmid collections covering a significant fraction of the virosphere are still needed. In this perspective, we present ViralORFeome 1.0 (http://www.viralorfeome.com), an open-access database and management system that provides an integrated set of bioinformatic tools to clone viral ORFs in the Gateway® system. ViralORFeome provides a convenient interface to navigate through virus genome sequences, to design ORF-specific cloning primers, to validate the sequence of generated constructs and to browse established collections of virus ORFs. Most importantly, ViralORFeome has been designed to manage all possible variants or mutants of a given ORF so that the cloning procedure can be applied to any emerging virus strain. A subset of plasmid constructs generated with ViralORFeome platform has been tested with success for heterologous protein expression in different expression systems at proteome scale. ViralORFeome should provide our community with a framework to establish a large collection of virus ORF clones, an instrumental resource to determine functions, activities and binding partners of viral proteins

The Spot-Forward Exchange Rate Relation in Indian Foreign Exchange Market An Analysis

Forward exchange rate bias explanation generally falls into two categories – assumption of rational expectation resulting in a risk premium and expectation errors which is systematic. The paper tests the bias in the Indian forward exchange markets using one-month and three month forward contracts. The study finds that the three month contracts have larger prediction errors than the one-month contracts. The also paper finds that the prediction errors have information content which leads to assume the presence of risk premium. The study also finds that risk one-month contracts have lesser variability vis-à-vis the three month contracts

Monitoring small scale fisheries (canoe) trajectories in West Africa: interest for management and research perspective [résumé]

ICAWA : International Conference AWA, Dakar, SEN, 13-/12/2016 - 15/12/2016The West African small scale fisheries is often the main component vs industrial fisheries in biomass landing. More over the small scale fisheries is of major interest at socio economics level and is sometime a cultural activity. In Senegal the number of canoe operating in the sub region is around 16 000 to 22 000 canoes according to the source. Such huge fleet is particularly complex to manage and if industrial fishing fleet can be monitored with ad hoc system as VMS (Vessel Monitoring System) this is not the case (technical limitation) for fishermen canoe. In this work we present the results of a demonstration project showing the interest to monitor the canoe fishing trip using low cost portable autonomous global positioning system (GPS) developed by IRD. During one year some trial have been carry out in Hann a landing site of Dakar (Senegal). Preliminary results show that at a high temporal resolution the GPS allow to distinguish the fishing operation (e.g. line and seine) from the transit and exploration route. Such data allow to improve the estimation of the small scale fisheries fishing effort but also allow to know the maximum distance of operation to check potential interferences with the industrial fishing area (usually between around 6 to 10 nmi). We also report that fishermen based in a landing site can fish in another area and even land is catch in a third landing site, thus ecological and fisheries study made from data collected in landing site must be analyzed with care because au bias that such fishermen behavior can introduce in fisheries studies. The analysis of fishermen kinematics allow to improve the estimation of catchability coefficient, the estimation of economics costs, and allow to set spatial Predator/prey model. Moreover the migration of fisherman from local to regional, which is sensitive in the sub region can be monitored. Conservation authorities can also use this information to check Marine Protected Area frequentation as well as local fishing committees the activities in the protected fishing area (ZPP). We want to underline the interest of such data in fisheries management which can also with little technical improvement play an important role for fishermen security at sea with the implementation of rescue system but also could allow participative data collection (physics and chemical parameters as well as fish sample) from small scale fisheries for manager and fisheries scientist. At the time of miniaturization of electronic component and big data analysis, we recommend the equipment of such system at large scale for small scale fisheries in West Africa

International conference ICAWA 2016 : extended book of abstract : the AWA project : ecosystem approach to the management of fisheries and the marine environment in West African waters

The West African small scale fisheries is often the main component vs industrial fisheries in biomass landing. More over the small scale fisheries is of major interest at socio economics level and is sometime a cultural activity. In Senegal the number of canoe operating in the sub region is around 16 000 to 22 000 canoes according to the source. Such huge fleet is particularly complex to manage and if industrial fishing fleet can be monitored with ad hoc system as VMS (Vessel Monitoring System) this is not the case (technical limitation) for fishermen canoe. In this work we present the results of a demonstration project showing the interest to monitor the canoe fishing trip using low cost portable autonomous global positioning system (GPS) developed by IRD. During one year some trial have been carry out in Hann a landing site of Dakar (Senegal). Preliminary results show that at a high temporal resolution the GPS allow to distinguish the fishing operation (e.g. line and seine) from the transit and exploration route. Such data allow to improve the estimation of the small scale fisheries fishing effort but also allow to know the maximum distance of operation to check potential interferences with the industrial fishing area (usually between around 6 to 10 nmi). We also report that fishermen based in a landing site can fish in another area and even land is catch in a third landing site, thus ecological and fisheries study made from data collected in landing site must be analyzed with care because au bias that such fishermen behavior can introduce in fisheries studies. The analysis of fishermen kinematics allow to improve the estimation of catchability coefficient, the estimation of economics costs, and allow to set spatial Predator/prey model. Moreover the migration of fisherman from local to regional, which is sensitive in the sub region can be monitored. Conservation authorities can also use this information to check Marine Protected Area frequentation as well as local fishing committees the activities in the protected fishing area (ZPP). We want to underline the interest of such data in fisheries management which can also with little technical improvement play an important role for fishermen security at sea with the implementation of rescue system but also could allow participative data collection (physics and chemical parameters as well as fish sample) from small scale fisheries for manager and fisheries scientist. At the time of miniaturization of electronic component and big data analysis, we recommend the equipment of such system at large scale for small scale fisheries in West Africa