Wavelength dependent ac-Stark shift of the 1S0 - 3P1 transition at 657 nm in Ca

We have measured the ac-Stark shift of the 4s2 1S0 - 4s4p 3P1 line in 40Ca for perturbing laser wavelengths between 780 nm and 1064 nm with a time domain Ramsey-Borde atom interferometer. We found a zero crossing of the shift for the mS = 0 - mP = 0 transition and \sigma polarized perturbation at 800.8(22) nm. The data was analyzed by a model deriving the energy shift from known transition wavelengths and strengths. To fit our data, we adjusted the Einstein A coefficients of the 4s3d 3D - 4s4p 3P and 4s5s 3S - 4s4p 3P fine structure multiplets. With these we can predict vanishing ac-Stark shifts for the 1S0 m = 0 - 3P1 m = 1 transition and \sigma- light at 983(12) nm and at 735.5(20) nm for the transition to the 3P0 level.Comment: 8 pages, 5 figures, 2 table

Resonance Fluorescence Spectrum of a Trapped Ion Undergoing Quantum Jumps

We experimentally investigate the resonance fluorescence spectrum of single 171Yb and 172Yb ions which are laser cooled to the Lamb-Dicke regime in a radiofrequency trap. While the fluorescence scattering of 172Yb is continuous, the 171Yb fluorescence is interrupted by quantum jumps because a nonvanishing rate of spontaneous transitions leads to electron shelving in the metastable hyperfine sublevel 2D3/2(F=2). The average duration of the resulting dark periods can be varied by changing the intensity of a repumping laser field. Optical heterodyne detection is employed to analyze the fluorescence spectrum near the Rayleigh elastic scattering peak. It is found that the stochastic modulation of the fluorescence emission by quantum jumps gives rise to a Lorentzian component in the fluorescence spectrum, and that the linewidth of this component varies according to the average duration of the dark fluorescence periods. The experimental observations are in quantitative agreement with theoretical predictions.Comment: 14 pages including 4 figures, pdf file, fig.1 replace

General Relativity in Electrical Engineering

In electrical engineering metamaterials have been developed that offer unprecedented control over electromagnetic fields. Here we show that general relativity lends the theoretical tools for designing devices made of such versatile materials. Given a desired device function, the theory describes the electromagnetic properties that turn this function into fact. We consider media that facilitate space-time transformations and include negative refraction. Our theory unifies the concepts operating behind the scenes of perfect invisibility devices, perfect lenses, the optical Aharonov-Bohm effect and electromagnetic analogs of the event horizon, and may lead to further applications

Performance of a 229 Thorium solid-state nuclear clock

The 7.8 eV nuclear isomer transition in 229 Thorium has been suggested as an etalon transition in a new type of optical frequency standard. Here we discuss the construction of a "solid-state nuclear clock" from Thorium nuclei implanted into single crystals transparent in the vacuum ultraviolet range. We investigate crystal-induced line shifts and broadening effects for the specific system of Calcium fluoride. At liquid Nitrogen temperatures, the clock performance will be limited by decoherence due to magnetic coupling of the Thorium nucleus to neighboring nuclear moments, ruling out the commonly used Rabi or Ramsey interrogation schemes. We propose a clock stabilization based on counting of flourescence photons and present optimized operation parameters. Taking advantage of the high number of quantum oscillators under continuous interrogation, a fractional instability level of 10^{-19} might be reached within the solid-state approach.Comment: 28 pages, 9 figure

Density Matrix Kinetic Equation Describing a Passage of Fast Atomic Systems Through Matter

The quantum-mechanical consideration of a passage of fast dimesoatoms through matter is given. A set of quantum-kinetic equations for the density matrix elements describing their internal state evolution is derived. It is shown that probabilistic description of internal dynamics of hydrogen-like atoms is impossible even at sufficiently low energies because of the ``accidental'' degeneracy of their energy levels.Comment: 12 pages, LATEX, submitted to J. Phys.

Impacts of organic and conventional crop management on diversity and activity of free-living nitrogen fixing bacteria and total bacteria are subsidiary to temporal effects

A three year field study (2007-2009) of the diversity and numbers of the total and metabolically active free-living diazotophic bacteria and total bacterial communities in organic and conventionally managed agricultural soil was conducted at the Nafferton Factorial Systems Comparison (NFSC) study, in northeast England. The result demonstrated that there was no consistent effect of either organic or conventional soil management across the three years on the diversity or quantity of either diazotrophic or total bacterial communities. However, ordination analyses carried out on data from each individual year showed that factors associated with the different fertility management measures including availability of nitrogen species, organic carbon and pH, did exert significant effects on the structure of both diazotrophic and total bacterial communities. It appeared that the dominant drivers of qualitative and quantitative changes in both communities were annual and seasonal effects. Moreover, regression analyses showed activity of both communities was significantly affected by soil temperature and climatic conditions. The diazotrophic community showed no significant change in diversity across the three years, however, the total bacterial community significantly increased in diversity year on year. Diversity was always greatest during March for both diazotrophic and total bacterial communities. Quantitative analyses using qPCR of each community indicated that metabolically active diazotrophs were highest in year 1 but the population significantly declined in year 2 before recovering somewhat in the final year. The total bacterial population in contrast increased significantly each year. Seasonal effects were less consistent in this quantitative study

A Tethered Bilayer Assembled on Top of Immobilized Calmodulin to Mimic Cellular Compartmentalization

International audienceBACKGROUND: Biomimetic membrane models tethered on solid supports are important tools for membrane protein biochemistry and biotechnology. The supported membrane systems described up to now are composed of a lipid bilayer tethered or not to a surface separating two compartments: a "trans" side, one to a few nanometer thick, located between the supporting surface and the membrane; and a "cis" side, above the synthetic membrane, exposed to the bulk medium. We describe here a novel biomimetic design composed of a tethered bilayer membrane that is assembled over a surface derivatized with a specific intracellular protein marker. This multilayered biomimetic assembly exhibits the fundamental characteristics of an authentic biological membrane in creating a continuous yet fluid phospholipidic barrier between two distinct compartments: a "cis" side corresponding to the extracellular milieu and a "trans" side marked by a key cytosolic signaling protein, calmodulin. METHODOLOGY/PRINCIPAL FINDINGS: We established and validated the experimental conditions to construct a multilayered structure consisting in a planar tethered bilayer assembled over a surface derivatized with calmodulin. We demonstrated the following: (i) the grafted calmodulin molecules (in trans side) were fully functional in binding and activating a calmodulin-dependent enzyme, the adenylate cyclase from Bordetella pertussis; and (ii) the assembled bilayer formed a continuous, protein-impermeable boundary that fully separated the underlying calmodulin (trans side) from the above medium (cis side). CONCLUSIONS: The simplicity and robustness of the tethered bilayer structure described here should facilitate the elaboration of biomimetic membrane models incorporating membrane embedded proteins and key cytoplasmic constituents. Such biomimetic structures will also be an attractive tool to study translocation across biological membranes of proteins or other macromolecules

Yersinia enterocolitica Serum Resistance Proteins YadA and Ail Bind the Complement Regulator C4b-Binding Protein

Many pathogens are equipped with factors providing resistance against the bactericidal action of complement. Yersinia enterocolitica, a Gram-negative enteric pathogen with invasive properties, efficiently resists the deleterious action of human complement. The major Y. enterocolitica serum resistance determinants include outer membrane proteins YadA and Ail. Lipopolysaccharide (LPS) O-antigen (O-ag) and outer core (OC) do not contribute directly to complement resistance. The aim of this study was to analyze a possible mechanism whereby Y. enterocolitica could inhibit the antibody-mediated classical pathway of complement activation. We show that Y. enterocolitica serotypes O:3, O:8, and O:9 bind C4b-binding protein (C4bp), an inhibitor of both the classical and lectin pathways of complement. To identify the C4bp receptors on Y. enterocolitica serotype O:3 surface, a set of mutants expressing YadA, Ail, O-ag, and OC in different combinations was tested for the ability to bind C4bp. The studies showed that both YadA and Ail acted as C4bp receptors. Ail-mediated C4bp binding, however, was blocked by the O-ag and OC, and could be observed only with mutants lacking these LPS structures. C4bp bound to Y. enterocolitica was functionally active and participated in the factor I-mediated degradation of C4b. These findings show that Y. enterocolitica uses two proteins, YadA and Ail, to bind C4bp. Binding of C4bp could help Y. enterocolitica to evade complement-mediated clearance in the human host