Bell lysaker emotion recognition test: a contribution for the italian validation

INTRODUCTION: Emotion recognition deficits in psychopathology have been extensively studied with a variety of measures. The Bell Lysaker Emotion Recognition Test (BLERT; Bell et al., 1997) is an effective method to assess emotion recognition by presenting affect stimuli which may have greater verisimilitude with real life events. Indeed, BLERT combines facial expressions with affective information transmitted in prosody or body posture. This method has allowed the study of emotion recognition deficit in psychotic patients, as well as its relationships with other aspects of psychopathology (Vohs et al., 2014). OBJECTIVES: We aimed at testing the validity and reliability of an Italian version of the BLERT. AIMS: First, a group-comparison was carried out between clinical and nonclinical participants. Then, correlations among BLERT scores and other indices of psychological functioning were explored. METHODS: We recruited 12 inpatients with psychotic disorders (mean age= 54.75; 58.3% female) and 45 nonclinical participants (mean age= 24.04; 75.6% female). We administered the BLERT (Bell et al., 1997), along with the following measures: Empathy Quotient (Lawrence et al., 2004), Interpersonal Reactivity Index (Davis, 1980), Difficulties in Emotion Regulation Scale (Gratz & Roemer, 2004), and the Inventory of Interpersonal Problems-47 (Pilkonis et al., 1996). RESULTS: Clinical participants resulted impaired in all indices of the BLERT. Further, the construct validity of the BLERT was confirmed by associations with measures of empathy, emotion dysregulation, and interpersonal problems. CONCLUSIONS: The use of the Italian version of the BLERT seemed promising for the study of emotion recognition in both clinical and nonclinical samples

Noncommutative gravity with self-dual variables

We build a noncommutative extension of Palatini-Holst theory on a twist-deformed spacetime, generalizing a model that has been previously proposed by Aschieri and Castellani. The twist deformation entails an enlargement of the gauge group, and leads to the introduction of new gravitational degrees of freedom. In particular, the tetrad degrees of freedom must be doubled, thus leading to a bitetrad theory of gravity. The model is shown to exhibit new duality symmetries. The introduction of the Holst term leads to a dramatic simplification of the dynamics, which is achieved when the Barbero-Immirzi parameter takes the value $\beta=-i$, corresponding to a self-dual action. We study in detail the commutative limit of the model, focusing in particular on the role of torsion and non-metricity. The effects of spacetime noncommutativity are taken into account perturbatively, and are computed explicitly in a simple example. Connections with bimetric theories and the role of local conformal invariance in the commutative limit are also explored.Comment: 52 pages; v2: added references, corrected minor typos; matches published versio

Design of a Monitoring Tool for Eco-Regions

An Eco-Region (Biodistretto in Italian) is a rural area where different actors work together for the sustainable management of local resources, based on the principles and models of organic farming. Social, environmental, economic as well as ethical dimensions are involved. Eco-Regions are quickly growing in number in the EU and worldwide; different Eco-Regions types emerged, showing a variety of cultural, physical, socio-economic characteristics, and related policies and regulations. The Eco-Regions represent an innovative sustainable integrated rural development approach; their success can be supported by analyzing their basic features and their development dynamics. A balance between a strictly centralized and bureaucratic Eco-Regions management and a bottom-up non-regulated Eco-Regions proliferation should be provided to grant the development of community-based resilient initiatives. To this end a monitoring tool, based on the Porter's Diamond model, involving the local actors in analyzing the Eco-Regions structure and performances, could provide useful. Previous studies defined an Eco-Region monitoring tool, where different shortcomings characterized the analytical framework definition. The goal of the present paper is therefore to define an improved monitoring tool, more consistent to the Porter's Diamond model by reviewing the cluster related literature, focusing on the Porter's approach. The integrations resulting from the literature analysis contributed to a major increase in the indicators directly related to the original Porter's approach; new food security and sovereignty, as well as specifically designed sustainability indicators have also been added. By expanding the pool of indicators, the monitoring tool is more adaptive and able to support sustainable management of Eco-Regions in different contexts

RANBP2 (RAN binding protein 2)

Review on RANBP2 (RAN binding protein 2) , with data on DNA, on the protein encoded, and where the gene is implicated

Mitotic cell death induction by targeting the mitotic spindle with tubulin-inhibitory indole derivative molecules

Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division. The final fates of microtubule-inhibited cells are however often heterogeneous and difficult to predict. While recent work has provided insight into the cell response to inhibitors of microtubule dynamics (taxanes), the cell response to tubulin polymerization inhibitors remains less well characterized. Arylthioindoles (ATIs) are recently developed tubulin inhibitors. We previously identified ATI members that effectively inhibit tubulin polymerization in vitro and cancer cell growth in bulk cell viability assays. Here we characterise in depth the response of cancer cell lines to five selected ATIs. We find that all ATIs arrest mitotic progression, yet subsequently yield distinct cell fate profiles in time-lapse recording assays, indicating that molecules endowed with similar tubulin polymerization inhibitory activity in vitro can in fact display differential efficacy in living cells. Individual ATIs induce cytological phenotypes of increasing severity in terms of damage to the mitotic apparatus. That differentially triggers MCL-1 down-regulation and caspase-3 activation, and underlies the terminal fate of treated cells. Collectively, these results contribute to define the cell response to tubulin inhibitors and pinpoint potentially valuable molecules that can increase the molecular diversity of tubulin-targeting agents

Evaluation of intestinal bacterial flora of conventional and organic broilers using culture-based methods

The major bacteria colonizing the intestinal tract (ileum and caecum) of organic (O) and conventional (C) chickens were counted, isolated and identified by conventional methods. Chickens were obtained from 7 conventional and 7 organic chicken farms (n=203). Intestinal sampling was performed at different ages, every 10 days, starting at 20 days until 40 and from 20 days to 80 days of age, respectively, for conventional and organic birds. Statistical analysis was performed on two separate data sets (40 days of age and all ages).The comparison of C vs O systems was analyzed with univariate and multivariate procedures. There were large differences in bacterial counts in relation to the portion of intestine, the rearing system and the farms. In the ileum of conventional birds Enterobacteria were higher than in organic birds (7.03 vs 6.09 CFUxlog/g; P<0.05), whereas the contrary was observed for Lactobacilli (6.75 vs 7.07 CFUxlog/g; P<0.05). With respect to the other microflora, the effect of farm probably masked possible differences. The effect of rearing system was more visible in the caecum than in the ileum: Enterobacteria levels were higher in C than in O chickens (7.42 vs 7.05 CFUxlog/g; P<0.01), whereas Enterococci (7.65 vs 6.55 CFUxlog/g; P<0.05), Lactobacilli (7.85 vs 7.31 CFUxlog/g; P<0.05) and total aerobia (8.12 vs 7.66 CFUxlog/g; P<0.01) counts were higher in organic chickens. Multivariate analysis of caecum microflora showed the possibility of discriminating the rearing system. In the ileum of conventional birds Enterobacteria and total aerobia increased with age, while Lactobacilli decreased. In the O system, Enterobacteria, Lactobacilli and total anaerobia showed a similar trend, whereas total aerobia and Enterococci showed the opposite trend. A similar situation was observed in the caecum. Further investigations are necessary to better assess the role and effect of the enteric flora on the productive performance and on the health status of reared chickens

G-CSF–stimulated Neutrophils Are a Prominent Source of Functional BLyS

B lymphocyte stimulator (BLyS) is a novel member of the TNF ligand superfamily that is important in B cell maturation and survival. We demonstrate that human neutrophils, after incubation with G-CSF or, less efficiently, IFNγ, express high levels of BLyS mRNA and release elevated amounts of biologically active BLyS. In contrast, surface expression of the membrane-bound BLyS was not detected in activated neutrophils. Indeed, in neutrophils, uniquely among other myeloid cells, soluble BLyS is processed intracellularly by a furin-type convertase. Worthy of note, the absolute capacity of G-CSF–stimulated neutrophils to release BLyS was similar to that of activated monocytes or dendritic cells, suggesting that neutrophils might represent an important source of BLyS. In this regard, we show that BLyS serum levels as well as neutrophil-associated BLyS are significantly enhanced after in vivo administration of G-CSF in patients. In addition, serum obtained from two of these patients induced a remarkable accumulation of neutrophil-associated BLyS in vitro. This effect was neutralized by anti–G-CSF antibodies, indicating that G-CSF, present in the serum, stimulated neutrophils to produce BLyS. Collectively, our findings suggest that neutrophils, through the production of BLyS, might play an unsuspected role in the regulation of B cell homeostasis

Prognostic and Functional Significant of Heat Shock Proteins (HSPs) in Breast Cancer Unveiled by Multi-Omics Approaches

Heat shock proteins (HSPs) are a well-characterized molecular chaperones protein family, classified into six major families, according to their molecular size. A wide range of tumors have been shown to express atypical levels of one or more HSPs, suggesting that they could be used as biomarkers. However, the collective role and the possible coordination of HSP members, as well as the prognostic significance and the functional implications of their deregulated expression in breast cancer (BC) are poorly investigated. Here, we used a systematic multi-omics approach to assess the HSPs expression, the prognostic value, and the underlying mechanisms of tumorigenesis in BC. By using data mining, we showed that several HSPs were deregulated in BC and significantly correlated with a poor or good prognosis. Functional network analysis of HSPs co-expressed genes and miRNAs highlighted their regulatory effects on several biological pathways involved in cancer progression. In particular, these pathways concerned cell cycle and DNA replication for the HSPs co-expressed genes, and miRNAs up-regulated in poor prognosis and Epithelial to Mesenchymal Transition (ETM), as well as receptors-mediated signaling for the HSPs co-expressed genes upregulated in good prognosis. Furthermore, the proteomic expression of HSPs in a large sample-set of breast cancer tissues revealed much more complexity in their roles in BC and showed that their expression is quite variable among patients and confined into different cellular compartments. In conclusion, integrative analysis of multi-omics data revealed the distinct impact of several HSPs members in BC progression and indicate that collectively they could be useful as biomarkers and therapeutic targets for BC management

Post-operative computed tomography imaging evaluation of ascending aorta surgery

Ascending thoracic aorta disease is often a life-threatening condition. Aortic aneurysm and aortic dissection are the most frequent ascending aorta diseases requiring surgical intervention. Surgical repair techniques of the ascending aorta are various; they include reconstruction of the ascending aorta by using a graft with or without a prosthetic valve, reconstruction with a composite artificial graft or using a biological graft, and reconstruction of the ascending aorta with a composite graft preserving the native valve and arch repair. The radiologist plays a key role in the identification of post-operative complications; differentiation from normal postoperative findings is fundamental. Our aim is to discuss the main diseases affecting the ascending aorta requiring surgery and the different techniques used to treat them. We also discuss the normal computed tomography (CT) imaging findings and after-surgery complications