Humoral immune response of pregnant goats to two Corynebacterium pseudotuberculosis bacterin formulations

Caseous lymphadenitis is a chronic suppurative bacterial disease caused by Corynebacterium pseudotuberculosis, and it has a high prevalence among small ruminants. Different vaccine preparations have been employed to control this disease. The adjuvant used in the formulation is a critical factor for the improvement of vaccine efficacy. No commercial vaccines are available in Argentina. The aims of the present work were to compare the specific antibody response against a C. pseudotuberculosis whole cell vaccine formulated with a classical and an immunoestimulant adjuvant in pregnant goats, and to assess the transfer of vaccine-specific antibodies from the goat to the offspring through colostrum. Twenty three pregnant goats were vaccinated. The animals were randomly allocated to three groups: control, aluminum hydroxide Al(OH)3 adjuvant and a new cage-like particle adjuvant (ISPA). Antibodies (IgG) against this antigen were measured by ELISA. A robust humoral immune response was detected in vaccinated animals, with production of specific IgG. No significant differences were found between the vaccines formulated with different adjuvants. High levels of specific antibodies were detected in colostrum samples from both immunised groups, which were passively transferred from goats to offprings. No adverse local reactions in the vaccines injection sites were detected

Humoral immune response of pregnant goats to two Corynebacterium pseudotuberculosis bacterin formulations

Caseous lymphadenitis is a chronic suppurative bacterial disease caused by Corynebacterium pseudotuberculosis with a high prevalence in small ruminants. Different vaccine preparations have been employed to control this disease. The adjuvant used in the formulation is a critical factor for the improvement of vaccine efficacy. No commercial vaccines are available in Argentina. The aims of the present work were to compare the specific antibody response against a C. pseudotuberculosis whole cell vaccine formulated with a classical and an immunoestimulant adjuvant in pregnant goats, and to assess the transfer of vaccine- specific antibodies from the goat to the offspring through colostrum. Twenty three pregnant goats were vaccinated. The animals were randomly allocated to three groups: control, aluminum hydroxide Al(OH)3 adjuvant and a new cage-like particle adjuvant (ISPA). Antibodies (IgG) against this antigen were measured by ELISA. A robust humoral immune response was detected in vaccinated animals, with production of specific IgG, without finding significant differences between the vaccines formulated with different adjuvants. High levels of specific antibodies were detected in colostrum samples from both immunized groups, which were passively transfered from goats to offprings. No adverse local reactions in the vaccines injection sites were detected.Fil: Auad, Jordana. Universidad Católica de Córdoba; ArgentinaFil: Cerutti, Julieta. Universidad Católica de Córdoba; ArgentinaFil: Cooper, Laura Gabriela. Universidad Católica de Córdoba; ArgentinaFil: Camussone, Cecilia María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lozano, Natalia A.. Universidad Católica de Córdoba; ArgentinaFil: Crespo, Francisco M.. Establecimiento Los Talas. Potrero de Garay, Córdoba; ArgentinaFil: Lozano, Alejandro. Universidad Católica de Córdoba; Argentin

Factores de virulencia de Staphylococcus aureus asociados con infecciones mamarias en bovinos: relevancia y rol como agentes inmunógenos

Staphylococcus aureus es el microorganismo causante de mastitis bovina más prevalente en Argentina y en el mundo. La falta de efectividad frente a este organismo de los métodos tradicionales de control, basados en la higiene y la terapia antibiótica, ha conducido a la búsqueda de alternativas para prevenir la enfermedad. Una de ellas es la manipulación de los mecanismos defensivos del huésped mediante vacunación. La identificación de los factores de virulencia que estimulan las defensas del huésped es fundamental para el desarrollo racional de inmunógenos.S. aureus posee múltiples factores de virulencia que interactúan con el huésped en distintas etapas de la infección mamaria; algunos de ellos han mostrado capacidad de generar una respuesta inmune benéfica para el huésped. El objetivo de este artículo es revisar conceptos de estructura, función y utilización como inmunógenos de los factores de virulencia de S. aureus considerados como más relevantes en las principales etapas de la infección intramamaria