Response to fungal exudates of the rhizosphere isolate Pseudomonas sp. UMAF110 involves a GGDEF/EAL domain-containing protein

Pseudomonas sp. UMAF110, isolated from rhizosphere soil in Spain, display in vitro antagonism towards the pythopathogenic fungus Rosellinia necatrix and is able grow in fungal exudates (BM-RE medium). A transposon mutant library of this strain was constructed and several mutants were selected by their reduced competitiveness in BM-RE medium. Pseudomonas sp. UMAF110-G3, which contains the transposon into a gene encoding a putative REC/PAS/GGDEF/EAL protein, was selected for further characterization. Blastn searches using the sequence of the gene interrupted by the transposon in UMAF110-G3, here called cmpA (c-di-GMP Metabolizing Protein), yielded a single positive hit (98% cover, 78% identity) with a gene from a terpene-degrading Pseudomonas sp. strain isolated from soil. Context analysis of the cmpA gene in Pseudomonas sp. UMAF110 showed that this gene is located downstream from several genes involved in flagellar motility/chemotaxis. RT-PCR experiments further confirmed that cmpA form a transcriptional unit with the che gene cluster. Expression analysis of cmpA by qRT-PCR clearly showed upregulation of this gene after transfer of Pseudomonas sp. UMAF110 cells to BM-RE medium, suggesting a role for this operon in response to fungal exudates. Deletion of cmpA in Pseudomonas sp. UMAF110 did not affect the ability of the strain to form biofilms under the conditions tested. However, overexpression of wild type CmpA in Pseudomonas putida KT2440 negatively regulated biofilm formation in this strain. Together, these results suggest that CmpA could be involved in signal transduction pathways regulating flagellar motility/chemotaxis in response to fungal exudates.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Microstructural evolution of a low-alloy steel / nickel superalloy dissimilar metal weld during post-weld heat treatment

The microstructural evolution of a dissimilar metal weld (DMW) obtained by narrow-gap gas tungsten arc welding (NG-GTAW) was investigated after it was subjected to a post-weld heat treatment (PWHT). The case studied here is a joint between low-alloy steel pipes and a stainless steel steam generator using a nickel based alloy as filler material. The fusion boundary that was the focus of this work was that between the low-alloy steel (2.25Cr-1Mo) and the nickel alloy (alloy 82). The difference in matrix phase and chemical composition between the two alloys leads to a large difference in chemical potential for carbon, which is mobile at the PWHT temperature. A number of advanced characterization techniques were used to assess the gradient of composition, hardness and microstructures across the fusion line, both as welded and after PWHT. This complete analysis permits to highlight and understand the main microstructural changes occurring during the PWHT

C. Plinii Secundi Librorum dubii sermonis VIII reliquiae

XXVII, 96 p

Estrutura populacional da raça Girolando.

RESUMO - O objetivo neste estudo foi avaliar a estrutura genética da população de bovinos da raça Girolando no Brasil. Analisou-se o arquivo de pedigree de 26.969 animais, composto de 3.031 machos e 23.938 fêmeas. O nível de conteúdo de informação do pedigree na geração atual foi 61%, mostrando ser de qualidade moderada. O coeficiente de endogamia médio e o coeficiente de relação médio da população Girolando foram 0,11 e 0,13%, respectivamente. O tamanho efetivo da população, considerando a geração completa traçada, foi 188, acima do nível crítico. Do total de 9.457 ancestrais que contribuíram para a população de referência, 457 explicaram 50% da variabilidade genética da população. O número efetivo de fundadores foi 551 e o de ancestrais 393. O intervalo médio de geração foi de 5,26 anos, sendo ligeiramente maior nas trilhas gaméticas mãe-filho e pai-filha. A partir dos coeficientes estimados, pode-se concluir que a endogamia nos rebanhos da raça Girolando foi de pequena magnitude e que as práticas de acasalamento foram adequadas durante o período avaliado. No entanto, é importante continuar com o monitoramento desses coeficientes a fim de prevenir perda de variabilidade genética. ABSTRACT - The aim of this study was to evaluate the population structure of Girolando cattle in Brazil. The pedigree file contained 26,969 individuals, from which 3,031 were males and 23,938 were females. The average level of completeness of the pedigree in the current generation was of reasonable quality (61%). Inbreeding and average relatedness coefficients were low: 0.11 and 0.13%, respectively. Estimates of effective population size considering the full generations traced was 188, which is above the critical level range. The number of ancestors that contributed to the reference population was 9,457 animals, from which 457 explained 50% of the genetic variability of the population. The effective number of founders and the effective number of ancestors in this population were, respectively, 551 and 393. The average generation interval was 5.26 years, slightly higher in genetic pathways dam-son and sire-daughter. The inbreeding in the Girolando breed was of small magnitude, indicating that the current practices of mating were adequate during the study period. However, it is important to continue monitoring these coefficients in order to prevent loss of genetic variability

Evaluación de la suplementación de creatina sobre el desempeño productivo y variables bioquímicas en lechones al destete

El destete es considerado como el período más estresante en la producción porcina y se asocia con trastornos intestinales, musculares y bajo rendimiento productivo. Estudios demuestran que la suplementación de creatina favorece la síntesis proteica. El objetivo de este trabajo de investigación fue evaluar el efecto de la suple- mentación de creatina en lechones mediante la medición del rendimiento productivo y pruebas bioquímicas en sangre. El trabajo de investigación se realizó en el Centro Experimental Uyumbicho (CEU) perteneciente a la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Central del Ecuador. Se utilizó un total de 16 lechones (Landrace x Yorkshire x Pietrain x Duroc). Los lechones se distribuyeron de manera aleatoria en cada uno de los 4 tratamientos: T1 = testigo 1 (sin suplemento lácteo), T2 = testigo 2 (suplemento lácteo), T3 = suplemento lácteo + creatina (0.5%), T4 = suplemento lácteo + creatina (1%). Los lechones que recibieron creatina al 1% presentaron el mayor consumo diario de alimento (0.432 kg/ día) y la mayor ganancia diaria de peso (0.336 kg/día) (42 días edad) y la mejor conversión alimenticia (1.288 kg/kg) (62 días de edad). La enzima creatina cinasa presentó el valor más elevado (2007.5 UL) en los lechones del grupo testigo 1. El uso de un sustituto lácteo y la suplementación de creatina mitiga los efectos adversos post-destete en lechones

Measurement of the B± production cross-section in pp collisions at s=7 and 13 TeV

The production of B± mesons is studied in pp collisions at centre-of-mass energies of 7 and 13 TeV, using B± → J/ψ K± decays and data samples corresponding to 1.0 fb−1 and 0.3 fb−1, respectively. The production cross-sections summed over both charges and integrated over the transverse momentum range 0 < pT < 40 GeV/c and the rapidity range 2.0 < y < 4.5 are measured to be σpp→B±X,s=7TeV=43.0±0.2±2.5±1.7μb, σpp→B±X,s=13TeV=86.6±0.5±5.4±3.4μb, where the first uncertainties are statistical, the second are systematic, and the third are due to the limited knowledge of the B± → J/ψ K± branching fraction. The ratio of the cross-section at 13 TeV to that at 7 TeV is determined to be 2.02 ± 0.02 (stat) ± 0.12 (syst). Differential cross-sections are also reported as functions of pT and y. All results are in agreement with theoretical calculations based on the state-of-art fixed next-to-leading order quantum chromodynamics

Measurement of the J/ψ pair production cross-section in pp collisions at s=13 TeV

The production cross-section of J/ψ pairs is measured using a data sample of pp collisions collected by the LHCb experiment at a centre-of-mass energy of s=13 TeV, corresponding to an integrated luminosity of 279 ±11 pb−1. The measurement is performed for J/ψ mesons with a transverse momentum of less than 10 GeV/c in the rapidity range 2.0 < y < 4.5. The production cross-section is measured to be 15.2 ± 1.0 ± 0.9 nb. The first uncertainty is statistical, and the second is systematic. The differential cross-sections as functions of several kinematic variables of the J/ψ pair are measured and compared to theoretical predictions