'Face Up to the Truth': Helping Gay Men in Vietnam Protect Themselves from AIDS.

Appropriate AIDS prevention information is not available in Vietnam for men who have sex with men. Current AIDS prevention messages can be misunderstood with potentially dangerous results. We outline some features of gay culture in a provincial city in Vietnam. We describe the activities of a peer educator who made contact with a small group of young gay men during 1996 and 1997. All the young men were ill-informed about AIDS. Their attitudes and sexual practices made them vulnerable to AIDS. The peer educator provided clear information and emotional support. The peer education was done without government endorsement and on a very low budget

Radiative transfer of synchrotron radiation through a compressed random magnetic field

The generally low polarization levels present in extragalactic radio sources suggest magnetic fields that are largely turbulent, presumably maintained by turbulence motion of the underlying plasma. The polarization of radio emission is often generated as the plasma is compressed by shock waves, and this type of configuration has been widely applied, from the cores of AGN to the lobes of classical double radio sources. Here we note that, in the absence of Faraday rotation, the synchrotron radiative transfer equations for such a configuration, separate, leading to relatively simple solutions for opaque or partially opaque sources. Expressions for the emission and absorption coefficients are derived, and, although in general, these must be evaluated numerically, the process is much simpler than a full numerical solution of the transfer equations

Multimodal nanoparticle imaging agents: Design and applications

Molecular imaging, where the location of molecules or nanoscale constructs can be tracked in the body to report on disease or biochemical processes, is rapidly expanding to include combined modality or multimodal imaging. No single imaging technique can offer the optimum combination of properties (e.g. resolution, sensitivity, cost, availability). The rapid technological advances in hardware to scan patients, and software to process and fuse images, are pushing the boundaries of novel medical imaging approaches, and hand-in-hand with this is the requirement for advanced and specific multimodal imaging agents. These agents can be detected using a selection from radioisotope, magnetic resonance and optical imaging, among others. Nanoparticles offer great scope in this area as they lend themselves, via facile modification procedures, to act as multifunctional constructs. They have relevance as therapeutics and drug delivery agents that can be tracked by molecular imaging techniques with the particular development of applications in optically guided surgery and as radiosensitizers. There has been a huge amount of research work to produce nanoconstructs for imaging, and the parameters for successful clinical translation and validation of therapeutic applications are now becoming much better understood. It is an exciting time of progress for these agents as their potential is closer to being realized with translation into the clinic. The coming 5–10 years will be critical, as we will see if the predicted improvement in clinical outcomes becomes a reality. Some of the latest advances in combination modality agents are selected and the progression pathway to clinical trials analysed. This article is part of the themed issue ‘Challenges for chemistry in molecular imaging’

Reduced maximum capacity of glycolysis in brown adipose tissue of genetically obese, diabetic (db/db) mice and its restoration following treatment with a thermogenic β-adrenoceptor agonist

AbstractThe maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic β-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in activity of hexokinase and 6-phosphofructokinase in the BRL 26830-treated db/db mice occurred in brown adipose tissue where the total tissue activity increased 10- and 11-fold respectively. These changes together with increased 2-deoxyglucose uptake in vivo suggest that brown adipose tissue can play a quantitatively important role in the removal of glucose from the blood

iShocks: X-ray binary jets with an internal shocks model

In the following paper we present an internal shocks model, iShocks, for simulating a variety of relativistic jet scenarios; these scenarios can range from a single ejection event to an almost continuous jet, and are highly user configurable. Although the primary focus in the following paper is black hole X-ray binary jets, the model is scale and source independent and could be used for supermassive black holes in active galactic nuclei or other flows such as jets from neutron stars. Discrete packets of plasma (or `shells') are used to simulate the jet volume. A two-shell collision gives rise to an internal shock, which acts as an electron re-energization mechanism. Using a pseudo-random distribution of the shell properties, the results show how for the first time it is possible to reproduce a flat/inverted spectrum (associated with compact radio jets) in a conical jet whilst taking the adiabatic energy losses into account. Previous models have shown that electron re-acceleration is essential in order to obtain a flat spectrum from an adiabatic conical jet: multiple internal shocks prove to be efficient in providing this re-energization. We also show how the high frequency turnover/break in the spectrum is correlated with the jet power, $\nu_b \propto L_{\textrm W}^{\sim 0.6}$, and the flat-spectrum synchrotron flux is correlated with the total jet power, $F_{\nu}\propto L_{\textrm W}^{\sim 1.4}$. Both the correlations are in agreement with previous analytical predictions.Comment: 13 pages, 17 figures, 5 tables. Accepted for publication in MNRA

Resonant-Cavity-Induced Phase Locking and Voltage Steps in a Josephson Array

We describe a simple dynamical model for an underdamped Josephson junction array coupled to a resonant cavity. From numerical solutions of the model in one dimension, we find that (i) current-voltage characteristics of the array have self-induced resonant steps (SIRS), (ii) at fixed disorder and coupling strength, the array locks into a coherent, periodic state above a critical number of active Josephson junctions, and (iii) when $N_a$ active junctions are synchronized on an SIRS, the energy emitted into the resonant cavity is quadratic with $N_a$. All three features are in agreement with a recent experiment [Barbara {\it et al}, Phys. Rev. Lett. {\bf 82}, 1963 (1999)]}.Comment: 4 pages, 3 eps figures included. Submitted to PRB Rapid Com

Adaptive filtering framework to remove nonspecific and low-efficiency reactions in multiplex digital PCR based on sigmoidal trends.

Real-time digital polymerase chain reaction (qdPCR) coupled with machine learning (ML) methods has shown the potential to unlock scientific breakthroughs, particularly in the field of molecular diagnostics for infectious diseases. One promising application of this emerging field explores single fluorescent channel PCR multiplex by extracting target-specific kinetic and thermodynamic information contained in amplification curves, also known as data-driven multiplexing. However, accurate target classification is compromised by the presence of undesired amplification events and not ideal reaction conditions. Therefore, here, we proposed a novel framework to identify and filter out nonspecific and low-efficient reactions from qdPCR data using outlier detection algorithms purely based on sigmoidal trends of amplification curves. As a proof-of-concept, this framework is implemented to improve the classification performance of the recently reported data-driven multiplexing method called amplification curve analysis (ACA), using available published data where the ACA is demonstrated to screen carbapenemase-producing organisms in clinical isolates. Furthermore, we developed a novel strategy, named adaptive mapping filter (AMF), to adjust the percentage of outliers removed according to the number of positive counts in qdPCR. From an overall total of 152,000 amplification events, 116,222 positive amplification reactions were evaluated before and after filtering by comparing against melting peak distribution, proving that abnormal amplification curves (outliers) are linked to shifted melting distribution or decreased PCR efficiency. The ACA was applied to assess classification performance before and after AMF, showing an improved sensitivity of 1.2% when using inliers compared to a decrement of 19.6% when using outliers (p-value < 0.0001), removing 53.5% of all wrong melting curves based only on the amplification shape. This work explores the correlation between the kinetics of amplification curves and the thermodynamics of melting curves, and it demonstrates that filtering out nonspecific or low-efficient reactions can significantly improve the classification accuracy for cutting-edge multiplexing methodologies

X-ray Properties of the GigaHertz-Peaked and Compact Steep Spectrum Sources

We present {\it Chandra} X-ray Observatory observations of Giga-Hertz Peaked Spectrum (GPS) and Compact Steep Spectrum (CSS) radio sources. The {\it Chandra} sample contains 13 quasars and 3 galaxies with measured 2-10 keV X-ray luminosity within $10^{42} - 10^{46}$ erg s$^{-1}$. We detect all of the sources, five of which are observed in X-ray for the first time. We study the X-ray spectral properties of the sample. The measured absorption columns in the quasars are different than those in the galaxies in the sense that the quasars show no absorption (with limits $\sim 10^{21} \rm cm^{-2}$) while the galaxies have large absorption columns ($> 10^{22} \rm cm^{-2}$) consistent with previous findings. The median photon index of the sources with high S/N is $\Gamma=1.84 \pm0.24$ and it is larger than the typical index of radio loud quasars. The arcsec resolution of {\it Chandra} telescope allows us to investigate X-ray extended emission, and look for diffuse components and X-ray jets. We found X-ray jets in two quasars (PKS 1127-145, B2 0738+32), an X-ray cluster surrounding a CSS quasar (z=1.1, 3C 186), detected a possible binary structure in 0941-080 galaxy and an extended diffuse emission in galaxy PKS B2 1345+12. We discuss our results in the context of X-ray emission processes and radio source evolution. We conclude that the X-ray emission in these sources is most likely unrelated to a relativistic jet, while the sources' radio-loudness may suggest a high radiative efficiency of the jet power in these sources.Comment: 15 pages, to be published in Ap

A Viewing Angle - Kinetic Luminosity Unification Scheme For BL Lacertae Objects

We propose a unified classification for BL Lac objects (BLs), focusing on the synchrotron peak frequency of the spectral energy distribution. The unification scheme is based on the angle Theta that describes the orientation of the relativistic jet and on the electron kinetic luminosity Lambda of the jet. We assume that Lambda scales with the size of the jet r in a self-similar fashion (Lambda propto r^2), as supported by observational data. The jets are self-similar in geometry and have the same pressure and median magnetic field at the inlet, independent of size. The self-similarity is broken for the highest energy electrons, which radiate mainly at high frequencies, since for large sources they suffer more severe radiative energy losses over a given fraction of the jet length. We calculate the optically thin synchrotron spectrum using an accelerating inner jet model based on simple relativistic gas dynamics and show that it can fit the observed infrared to X-ray spectrum of PKS 2155--304. We couple the accelerating jet model to the unification scheme and compare the results to complete samples of BLs. The negative apparent evolution of X-ray selected BLs is explained as a result of positive evolution of the jet electron kinetic luminosity $\Lambda_{kin}$. We review observational arguments in favor of the existence of scaled-down accretion disks and broad emission-line regions in BLs. The proposed unification scheme can explain the lack of observed broad emission lines in X-ray selected BLs, as well as the existence of those lines preferentially in luminous radio-selected BLs. Finally, we review observational arguments that suggest the extension of this unification scheme to all blazars.Comment: 32 pages, 8 figures, to be published in the ApJ (Oct 20, 1998