13 research outputs found
Regenerative endodontic procedure combined with apical surgery of a necrotic permanent incisor with extensive periapical lesion using plasma rich in growth factors (PRGF) : a case report with 6 years post-op evaluation using CBCT
The aim of this case report is to describe the step-by step and outcomes of a treatment approach which simultaneously combines a Regenerative Endodontic Procedure (REP) and apical surgery using PRGF as treatment of a post-traumatic necrotic permanent cen
The effect of glass ionomer and adhesive cements on substance P expression in human dental pulp
Objectives: The purpose of this study was to quantify the effect of glass ionomer and adhesive cements on SP
expression in healthy human dental pulp.
Study Design: Forty pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic
reasons. In thirty of these premolars a Class V cavity preparation was performed and teeth were equally divided
in three groups: Experimental Group I: Glass Ionomer cement was placed in the cavity. Experimental Group
II: Adhesive Cement was placed in the cavity. Positive control group: Class V cavities only. The remaining ten
healthy premolars where extracted without treatment and served as a negative control group. All pulp samples
were processed and SP was measured by radioimmunoassay.
Results: Greater SP expression was found in the adhesive cement group, followed by the glass ionomer and the
positive control groups. The lower SP values were for the negative control group. ANOVA showed statistically
significant differences between groups (p<0.0001). Tukey HSD post hoc tests showed statistically significant differences
in SP expression between negative control group and the 3 other groups (p<0.01). Differences between
the cavity-only group and the two experimental groups were also statistically significant (p<0.05 and p<0.01 respectively).
There is also a statistically significant difference between the two experimental groups (p<0.01).
Conclusions: These findings suggest that adhesive cements provoke a greater SP expression when compared with
glass ionomer
Comparative outcomes of platelet concentrates and blood clot scaffolds for regenerative endodontic procedures : a systematic review of randomized controlled clinical trials
The main objective of this systematic review is to evaluate the effectiveness of platelet concentrates -Platelet-rich plasma (PRP) or Fibrin-rich plasma (PRF)- compared with blood clot (BC) as scaffolds for maturogenesis, in patients with immature perman
Dentin growth after direct pulp capping with the different fractions of plasma rich in growth factors (PRGF) vs MTA: experimental study in animal model
Background: The study aimed to evaluate the area of dentin growth in rabbit incisors after pulp capping with plasma rich in growth factors (PRGF) compared with mineral trioxide aggregate (MTA) by fluorescence. Methods: 27 upper and lower incisors of rabbits were divided into 4 groups: poor PRGF (F1) (n = 9 teeth), rich PRGF (F2) (n = 8 teeth), ProRoot MTA (positive control, n = 5 teeth) and untreated (NC) (negative control, n=5). Fluorochrome markers were injected 24 hours before surgery and the day before euthanasia, 28 days after the vital pulp therapy (VPT). Two transverse cuts were performed to every tooth: the first cut (A), 1 mm incisal to the gingival margin, and the second cut (B), 5 mm apical to the first cut. The sections were assessed with histomorphometric evaluation by fluorescence microscopy, comparing the dentin area between fluorescence marks and the total mineralized area. Results: The higher percentage of dentin growth was observed in the F2 group (B=63.25%, A=36.52%), followed by F1 (B=57.63%, A=30,12%) and MTA (B=38.64%, A=15,74%) The group with lowest percentage of dentin growth was the NC group (B=29.22%, A=7.82%). Significant difference (p <0.05) was found between F2 group and MTA, also statistically significant difference has been observed comparing dentin growth areas of NC group with F1 and F2 groups. Conclusions: The application of PRGF rich and poor fraction as a pulp capping material stimulated dentin formation significantly more intensively than MTA and NC
Effectiveness, efficiency, and apical extrusion of 2 rotaries and 2 reciprocating systems in removing filling material during endodontic retreatment. A systematic review
This systematic review investigated the effectiveness, efficiency and apical extrusion of the debris of two rotary and two reciprocating single-file systems used for the removal of filling material from straight root canals. A literature search was per
The effect of orthodontic forces on calcitonin gene-related peptide (CGRP) expression in the human periodontal ligament and its relationship with the human dental pulp
The purpose of this study was to quantify the effect of moderate and severe orthodontic forces on Calcitonin gene-related peptide (CGRP) expression in the healthy human periodontal ligament (PDL) and its possible relationship with the human dental pulp
ExpresiĂłn de sustancia P, pĂ©ptido relacionado con el gen de la calcitonina y factor de crecimiento endotelial vascular en pulpa dental humana bajo diferentes estĂmulos clĂnicos
8 páginasBackground: The aim of this study was to measure the dental pulp infammatory response through neuropeptides
(SP and CGRP) as a response to occlusal trauma, orthodontic movements and a combination of both, as well as
the angiogenic defense mechanism through VEGF expression, which could be the initial step to mineralized tissue
formation.
Methods: Forty human dental pulp samples were collected from healthy frst premolars with extraction indicated
due to orthodontic reasons from a sample of 20 patients. Patients were divided into four groups with 10 premolars
each (1 mandibular and 1 maxillary premolar from each patient): healthy pulp control group, occlusal trauma group,
moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were
applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and
VEGF were measured by radioimmunoassay. The Kruskal–Wallis test was performed to assess signifcant diferences
among groups and Mann–Whitney’s U post hoc pairwise comparisons were also performed.
Results: The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic
forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statisti‑
cally signifcant diferences between all groups for each of the 3 peptides analyzed (Kruskal–Wallis p<0.001). All pos‑
sible pairwise post-hoc comparisons were also signifcant for each peptide analyzed (Mann–Whitney’s U p<0.001).
Conclusion: SP, CGRP, and VEGF expressions signifcantly increase in human dental pulps when stimulated
by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied
independentlyAntecedentes: El objetivo de este estudio fue medir la respuesta inflamatoria de la pulpa dental a través de neuropéptidos
(SP y CGRP) como respuesta a trauma oclusal, movimientos de ortodoncia y una combinaciĂłn de ambos, asĂ como
el mecanismo de defensa angiogĂ©nico a travĂ©s de la expresiĂłn de VEGF, que podrĂa ser el paso inicial hacia el tejido mineralizado
formaciĂłn.
MĂ©todos: Se recolectaron cuarenta muestras de pulpa dental humana de primeros premolares sanos con extracciĂłn indicada.
por motivos de ortodoncia a partir de una muestra de 20 pacientes. Los pacientes fueron divididos en cuatro grupos con 10 premolares
cada uno (1 premolar mandibular y 1 maxilar de cada paciente): grupo de control de pulpa sana, grupo de trauma oclusal,
grupo de fuerzas de ortodoncia moderadas; y grupo de trauma oclusal más fuerzas de ortodoncia moderadas. Los estĂmulos fueron
aplicado durante 24 h antes de la extracciĂłn del diente en todos los grupos experimentales. Todas las muestras fueron procesadas y SP, CGRP y
VEGF se midiĂł por radioinmunoensayo. Se realizĂł la prueba de Kruskal-Wallis para evaluar diferencias signifcativas
entre grupos y también se realizaron comparaciones por pares post hoc U de Mann-Whitney.
Resultados: El mayor incremento en las expresiones de SP, CGRP y VEGF se encontró en el trauma oclusal más ortodoncia.
grupo de fuerzas de ortodoncia, seguido por las fuerzas de ortodoncia moderadas, el trauma oclusal y los grupos de control, con estadĂsticas
diferencias significativas entre todos los grupos para cada uno de los 3 péptidos analizados (Kruskal-Wallis p<0,001). Todas las pos‑
Las posibles comparaciones post-hoc por pares también fueron significativas para cada péptido analizado (Mann-Whitney's Up p <0.001).
ConclusiĂłn: las expresiones de SP, CGRP y VEGF aumentan significativamente en las pulpas dentales humanas cuando se estimulan
por trauma oclusal combinado con fuerzas de ortodoncia moderadas, en comparaciĂłn con estos dos estĂmulos aplicados
independientement
Variation in the expression of insulin-like growth factor-1 in human pulp tissue according to the root-development stage
Insulin-like growth factor-1 (IGF-1) plays an important
role in cell proliferation and differentiation. It actively
participates during odontogenesis and mineralization
of dental hard tissues. The purpose of this study is to
use a radioimmunoassay (RIA) to determine whether
there are differences in the expression of IGF-1 in
human pulp from teeth having incomplete or complete
root development. Twenty-six pulp samples were obtained
from freshly extracted human third molars, half
of them from teeth with incomplete root development.
All of the samples were processed and labeled using an
IGF-1 RIA kit. Results showed IGF-1 presence in all
human pulp samples with a statistically significant
higher expression in pulps having complete root development.
The present findings are consistent with the
hypothesis that IGF-1 contributes toward forming and
mineralizing dental tissues in teeth with incomplete
root development as well as in pulp-repairing process in
teeth with complete root development
Quantification of Lactate-Dehydrogenase and Cell Viability in Postmortem Human Dental Pulp
Expression of insulin-like growth factor-1 and proliferating cell nuclear antigen in human pulp cells of teeth with complete and incomplete root development
Aim To quantify the expression of insulin-like growth
factor-1 (IGF-1) and proliferating cell nuclear antigen
(PCNA) in human pulp cells of teeth with complete or
incomplete root development, to support the specific
role of IGF-1 in cell proliferation during tooth development
and pulp reparative processes.
Methodology Twenty six pulp samples were
obtained from freshly extracted human third molars,
equally divided in two groups according to root
development stage (complete or incomplete root development).
All samples were processed and immunostained
to determine the expression of IGF-1 and PCNA
in pulp cells. Sections were observed with a light
microscope at 80· and morphometric analyses were
performed to calculate the area of PCNA and IGF-1
immunostaining using digital image software. Mann–
Whitney’s test was used to determine statistically
significant differences between groups (P < 0.05) for
each peptide and the co-expression of both.
Results Expression of IGF-1 and PCNA was observed
in all human pulp samples with a statistically significant
higher expression in cells of pulps having
complete root development (P = 0.0009).
Conclusion Insulin-like growth factor-1 and PCNA
are expressed in human pulp cells, with a significant
greater expression in pulp cells of teeth having
complete root development