Gestión de la calidad y la satisfacción del cliente de Deco Clean Express S.A.C., Lima 2022

La presente investigación tuvo como objetivo principal determinar la relación entre la gestión de la calidad y la satisfacción del cliente en la empresa Deco Clean Express S.A.C., Lima 2022., el cual se elaboró bajo el tipo de investigación aplicada, de enfoque cuantitativo, con un diseño no experimental correlacional de corte transversal, la muestra estuvo conformada por 73 clientes de la empresa Deco Clean Express S.A.C., para obtener y recolectar los datos se aplicó la técnica de la encuesta y el instrumento utilizado fue un cuestionario conformado por 42 preguntas, entre las variables de gestión de la calidad y satisfacción del cliente, ambas variables fueron validadas y aplicadas con una confiabilidad de 0.962 y 0.724 siendo aceptable. Se utilizó la estadística inferencial no paramétrica Rho de Spearman, para entender la relación de las variables y dimensiones aplicando el SPSS v.26. Los resultados mostraron que, el 47,9% de los clientes encuestados dijeron que en la empresa Deco Clean Express S.A.C., “Siempre” cumplen con la gestión de la calidad, además el 46,6% consideran que en Deco Clean Express S.A.C.,“A veces”, existe la satisfacción del cliente en la empresa, en conclusión, se evidencian que existe relación significativa entre la gestión de la calidad y la satisfacción del cliente con un nivel de significancia de 0,008 < 0.05 y un factor Rho de Spearman de 0.307 evidenciando que existe relación positiva baja en la empresa Deco Clean Express S.A.C

Resveratrol Enhances Polyubiquitination-Mediated ARV-7 Degradation in Prostate Cancer Cells

Although androgen deprivation therapy (ADT) serves as the primary treatment option for localized or metastatic prostate cancer, most cases eventually develop into castration-resistant prostate cancer (CRPC). However, androgen receptor (AR) continues to be functional in CRPC through various mechanisms, including the development of AR splicing variants, especially ARV7. Since it lacks the ligand binding domain but retains the intact DNA binding domain, ARV7 is constitutively active, which makes ARV7-positive prostate cancer responsive to neither abiraterone nor enzalutamide. In this study, we explored the effect of resveratrol on ARV7 transcriptional activity and the potential for development of resveratrol as a treatment for ARV7-positive prostate cancer. First, we ectopically expressed ARV7 in PC3 cells, an AR-negative prostate cancer cell line, and demonstrated that resveratrol is capable of inhibiting ARV7 transcriptional activity by downregulating ARV7 protein levels. Of note, resveratrol does not affect the mRNA levels of ARV7 nor its nuclear translocation. Next, we demonstrated that resveratrol is capable of downregulating the levels of the endogenously expressed ARV7 as well as AR target gene mRNAs in 22RV1 prostate cancer cells. Mechanistically, resveratrol downregulates ARV7 by enhancing ARV7 polyubiquitination and subsequent proteasome-mediated degradation. These findings suggest that resveratrol could be a potential treatment for ARV7-positive CPRC

Pre-treatment with grape seed extract reduces inflammatory response and oxidative stress induced by helicobacter pylori infection in human gastric epithelial cells

Helicobacter pylori (H. pylori) is a pathogenic bacteria identified as a potential risk factor for gastritis, gastric ulcers and gastric cancer. During the stomach colonization, H. pylori triggers a strong inflammatory response and subsequent oxidative stress, which are associated with tissue damage. For this reason, it is of particular interest to develop alternative natural tools that enable modulation of the associated damaging immune response. With this purpose, we obtained grape seed extract (GSE) from sweet (not fermented) food grade seeds. The aim of our study was to investigate the effect of GSE and its two enriched procyanidins fractions (OPC and PPC) on the inflammatory process and oxidative stress produced by different H. pylori strains in human gastric epithelial cells (AGS). Anti-inflammatory activity was evaluated by measuring the level of interleukin-8 (IL-8) secretion. IL-8 production was significantly reduced in H. pylori-infected human gastric epithelial cells pretreated with GSE or its enriched fractions when compared with non-pre-treated infected cells (from 21.6% to 87.8%). Pre-treatment with GSE or its fractions significantly decreased intracellular reactive oxygen species (ROS) production in AGS cells after infection, depending on the H. pylori strain. Our results also showed that GSE and its fractions demonstrate antibacterial activity against all strains of H. pylori used in the study. This work demonstrates the effectiveness of GSE enriched in procyanidins against the main events associated with H. pylori infection

Vacuum local and global electromagnetic self-energies for a point-like and an extended field source

We consider the electric and magnetic energy densities (or equivalently field fluctuations) in the space around a point-like field source in its ground state, after having subtracted the spatially uniform zero-point energy terms, and discuss the problem of their singular behavior at the source's position. We show that the assumption of a point-like source leads, for a simple Hamiltonian model of the interaction of the source with the electromagnetic radiation field, to a divergence of the renormalized electric and magnetic energy density at the position of the source. We analyze in detail the mathematical structure of such singularity in terms of a delta function and its derivatives. We also show that an appropriate consideration of these singular terms solves an apparent inconsistency between the total field energy and the space integral of its density. Thus the finite field energy stored in these singular terms gives an important contribution to the self-energy of the source. We then consider the case of an extended source, smeared out over a finite volume and described by an appropriate form factor. We show that in this case all divergences in local quantities such as the electric and the magnetic energy density, as well as any inconsistency between global and space-integrated local self-energies, disappear.Comment: 8 pages. The final publication is available at link.springer.co

Using Social Media as a Research Tool for a Bespoke Web-Based Platform for Stakeholders of Children With Congenital Anomalies: Development Study

BACKGROUND: Limited research evidence exists on the development of web-based platforms for reciprocal communication, coproduction research, and dissemination of information among parents, professionals, and researchers. This paper provides learning and the outcomes of setting up a bespoke web-based platform using social media. OBJECTIVE: This study aims to explore the establishment of a web-based, multicontextual research communication platform for parents and stakeholders of children with congenital anomalies using social media and to identify associated research and ethical and technical challenges. METHODS: The ConnectEpeople e-forum was developed using social media platforms with a stakeholder engagement process. A multilevel approach was implemented for reciprocal engagement between parents of children with congenital anomalies, researchers, health care professionals, and other stakeholders using private and invisible and public Facebook groups, closed Twitter groups, and YouTube. Ethical approval was obtained from Ulster University. RESULTS: Nonprofit organizations (N=128) were invited to engage with an initial response rate of 16.4% (21/128). Of the 105 parents contacted, 32 entered the private and invisible Facebook groups to participate in the coproduction research. Public Facebook page followers rose to 215, a total of 22 posts had an engagement of >10%, and 34 posts had a reach of over 100. Webinars included requested information on childhood milestones and behavior. YouTube coverage included 106 ConnectEpeople videos with 28,708 impressions. Project information was obtained from 35 countries. The highest Facebook activity occurred during the early morning hours. Achievement of these results required dedicated time management, social media expertise, creativity, and sharing knowledge to curate valuable content. CONCLUSIONS: Building and maintaining a multilayered online forum for coproduction and information sharing is challenging. Technical considerations include understanding the functionality and versatility of social media metrics. Social media offers valuable, easily accessible, quantitative, and qualitative data that can drive the reciprocal process of forum development. The identification and integration of the needs of the ConnectEpeople e-forum was a key driver in the dissemination of useful, meaningful, and accessible information. The necessary dedicated administration to respond to requests and posts and collate data required significant time and effort. Participant safety, the development of trust, and the maintenance of confidentiality were major ethical considerations. Discussions on social media platforms enabled parents to support each other and their children. Social media platforms are particularly useful in identifying common family needs related to early childhood development. This research approach was challenging but resulted in valuable outputs requiring further application and testing. This may be of particular importance in response to COVID-19 or future pandemics. Incorporating flexible, adaptable social media strategies into research projects is recommended to develop effective platforms for collaborative and impactful research and dissemination

Lucia Cavero

Lucia Cavero, DO 2020https://digitalcommons.pcom.edu/pa_2020_photos/1016/thumbnail.jp

Lucia Cavero, DO 2020

Philadelphia DO Class of 2020 portrait.https://digitalcommons.pcom.edu/portraits_2020/1040/thumbnail.jp

Effects of Resveratrol on Regulation of ARV7 Protein Levels in Prostate Cancer

Prostate cancer (PCa) has become a commonly diagnosed disease and it is the second-leading cause of cancer-related deaths in the US. A normal functioning prostate is androgen-dependent because it relies on androgens and the androgen receptor (AR) for cell maintenance, proliferation, survival and growth. Normally, the AR is a full-length receptor made up for 4 protein domains: N-terminal transactivation domain (NTD), a central DNA-binding domain (DBD), a hinge region and a C-terminal ligand-binding domain (LBD). Upon androgens binding to the LBD, activation of AR-FL occurs. The androgen-bound AR-FL forms a homodimer and translocates into the nucleus, where it binds to androgen-response elements (AREs) and regulates canonical AR-target genes such as prostate specific antigen (PSA). As PCa progresses to castration-resistant prostate cancer (CRPC), a more aggressive form of PCa, it shifts from being androgen-dependent to becoming androgen-independent. The change occurs because the AR is no longer a full-length receptor, but rather a variant (ARV). The most common variant is ARV7, a truncated protein that has lost the LBD where the androgen binds. As a result, ARV7 is constitutively active and can enhance tumor growth, independent of androgens. At this point resistance to current drug therapies and androgen deprivation therapy (ADT) is reached by PCa and CRPC patients. Recent work has shown that resveratrol, a polyphenol with antioxidant properties, has anti-cancerous effects in PCa by inducing apoptosis and inhibiting cell proliferation. However, the mechanism by which RSV provides these effects is not exactly known. Some studies show that RSV decreases expression of AR mRNA and AR protein levels post-translationally. Identification of the mechanism by which RSV exerts its effects on CRPC cells is crucial as new lines of therapeutic treatments for prostrate cancer are needed, due to the relapse after ADT and resistance developed to current drugs by PCa and CRPC patients. We hypothesize that RSV exerts its anti-cancerous properties in CRPC cell lines via specific ubiquitin degradation of ARV7 at a post-translational level. The 22RV1 cell line is CRPC. Cells were treated with RSV at different concentrations. 22RV1 cells were treated with MG132, a protease inhibitor, used to inhibit specific-ubiquitin degradation of ARV7. RT-PCR, qPCR and Western Blot were all performed. There is a dose-dependent effect of RSV on ARV7 mRNA and protein levels in CRPC. The effects of the combination of RSV/MG132 treatment on ARV7 protein levels seem comparable to the control, meaning that RSV might indeed regulate ARV7 protein levels via the ubiquitin degradation pathway

Presenting at Research Day

Lucia Cavero, DO \u2720, presents her research to Dr. Robert Barsotti at Research Day 2016.https://digitalcommons.pcom.edu/pa_2020_photos/1101/thumbnail.jp