29 research outputs found
Analysis Of The Ergosterol Biosynthesis Pathway Cloning, Molecular Characterization And Phylogeny Of Lanosterol 14 α-demethylase (erg11) Gene Of Moniliophthora Perniciosa
The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches’ broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed.ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.374683693Aime, M.C., Phillips-Mora, W., The causal agents of witches’ broom and frost pod rot of cacao (chocolate, Theobroma cacao) from a new lineage of Marasmiaceae (2005) Mycologia, 97, pp. 1012-1022Albertini, C., Thebaud, G., Fournier, E., Leroux, P., Eburicol 14α-demethylase gene (CYP51) polymorphism and speciation in Botrytis cinerea (2002) Mycol Res, 106, pp. 1171-1178Altschul, S.F., Gish, W., Miller, W., Myersewand Lipman, D.J., Basic local alignment search tool (1990) J Mol Biol, 215, pp. 403-410Bak, S., Kahn, R.A., Oisen, C.E., Halkier, B.A., Cloning and expression in Escherichia coli of the obtusifoliol 14α-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14α demethylases (CYP51) from fungi and mammals (1997) Plant J, 11, pp. 191-201Barrett-Bee, K., Dixon, G., Ergosterol biosynthesis inhibition: A target for antifungal agents (1995) Acta Biochim Pol, 42, pp. 465-480Bellamine, A., Mangla, A.T., Nes, W.D., Waterman, M.R., Characterization and catalytic properties of the sterol 14α-demethylase from Mycobacterium tuberculosis (1999) Proc Natl Acad Sci USA, 96, pp. 8937-8942Butler, G., Rasmussen, M.D., Lin, M.F., Santos, M.A., Sakthikumar, S., Munro, C.A., Rheinbay, E., Reedy, J.L., Evolution of pathogenicity and sexual reproduction in eight Candida genomes (2009) Nature, 459, pp. 657-662Carrillo-Muñoz, A.J., Giusiano, G., Ezkurra, P.A., Quindós, G., Antifungal agents: Mode of action in yeast cells (2006) Rev Esp Quim, 19, pp. 130-139Ceita, G.O., Macedo, J.N., Santos, T.B., Alemanno, L., Gesteira, A.S., Micheli, F., Mariano, A.C., Meinhardt, L.W., Involvement of calcium oxalate degradation during programmed cell death in Theobroma cacao tissues triggered by the hemibiotrophic fungus Moniliophthora perniciosa (2007) Plant Sci, 173, pp. 106-117D’souza, C.A., Kronstad, J.W., Taylor, G., Warren, R., Yuen, M., Hu, G., Jung, W.H., Tangen, K., Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts (2011) MBio, 2, pp. e00342-e00410Délye, C., Laigret, F., Corio-Costet, M.F., Cloning and sequence analysis of the eburicol 14α-demethylase gene of the obligate biotrophic grape powdery mildew fungus (1997) Gene, 195, pp. 29-33Dujon, B., Sherman, D., Fischer, G., Durrens, P., Casaregola, S., Lafontaine, I., De Montigny, J., Talla, E., Genome evolution in yeasts (2004) Nature, 430, pp. 35-44Evans, H.C., Cacao diseases - The trilogy revisited (2007) Phytopathology, 97, pp. 1640-1643Felsenstein, J., Confidence limits on phylogenies: An approach using the bootstrap (1985) Evolution, 39, pp. 783-791Formighieri, E.F., Tiburcio, R.A., Armas, E.D., Medrano, F.J., Shimo, H., Carels, N., GóEs Neto, A., Sardinha-Pinto, N., The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109 kb in size and contains a stable integrated plasmid (2008) Mycol Res, 112, pp. 1136-1152Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M.R., Appel, R.D., Bairoch, A., Protein identification and analysis tools on the ExPASy Server (2005) The Proteomics and Protocols Handbook, pp. 571-607. , In: Walker JM, Humana Press, TotowaGoffeau, A., Barrell, B.G., Bussey, H., Davis, R.W., Dujon, B., Feldmann, H., Galibert, F., Johnston, M., Life with 6000 genes (1996) Science, 265, pp. 2077-2082Griffith, G.W., Bravo-Velasquez, E., Wilson, F.J., Lewis, D.M., Hedger, J.N., Autecology and evolution of the witches’ broom pathogen (Crinipellis perniciosa) of cocoa (1994) The Ecology of Plant Pathogens, pp. 245-265. , In: Blakeman JP and Williamson B, CAB International, WallingfordGriffith, G.W., Nicholson, J., Neinninger, A., Birch, R., Witches’ brooms and frosty pods: Two major pathogens of cacao (2003) New Zeal J Bot, 41, pp. 423-435Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Res, 41, pp. 95-98Hof, H., Critical annotations to the use of azole antifungals for plant protection (2001) Antimicrob Agents Chemother, 45, pp. 2987-2990Jackson, C.J., Lamb, D.C., Marczylo, T.H., Parker, J.E., Manning, N.L., Kelly, D.E., Kelly, S.L., Conservation and cloning of CYP51: A sterol 14α-demethylase from Mycobacterium smegmatis (2003) Biochem Biophys Res Commun, 301, pp. 558-563James, T.Y., Kauff, F., Schoch, C.L., Matheny, P.B., Hofstetter, V., Cox, C.J., Celio, G., Miadlikowska, J., Reconstructing the early evolution of Fungi using a six-gene phylogeny (2006) Nature, 19, pp. 818-822Jones, T., Federspiel, N.A., Chibana, H., Dungan, J., Kalman, S., Magee, B.B., Newport, G., Magee, P.T., The diploid genome sequence of Candida albicans (2004) Proc Natl Acad Sci USA, 101, pp. 7329-7334Kairuz, P.B., Zuber, J.P., Jaunin, P., Buchman, T.G., Bille, J., Rossier, M., Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-α-demethylase (L1A1) gene fragment (1994) J Clin Microbiol, 32, pp. 1902-1907Kalb, V.F., Woods, C.W., Turi, T.G., Dey, C.R., Sutter, T.R., Loper, J.C., Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae (1987) DNA, 6, pp. 529-537Kall, L., Krogh, A., Sonnhammer, E.L., Advantages of combined transmembrane topology and signal peptide prediction- the Phobius web server (2007) Nucleic Acids Res 35:429-, p. 432Kim, D., Lim, Y.R., Ohk, S.O., Kim, B.J., Chun, Y.J., Functional expression and characterization of CYP51 from dandruffcausing Malassezia globosa (2011) FEMS Yeast Res, 11, pp. 80-87Lai, M.H., Kirsch, D.R., Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14α-demethylase) from Candida albicans (1989) Nucleic Acids Res, 17, p. 804Lamb, D.C., Kelly, D.E., Manning, N.M., Hollomon, D.W., Kelly, S.L., Expression, purification, reconstitution and inhibition of Ustilago maydis sterol 14α-demethylase (CYP 51P450) (1998) FEMS Microbiol Lett, 169, pp. 369-373Lee, C.H., Hsu, K.H., Wang, S.Y., Chang, T.T., Chu, F.H., Shaw, J.F., Cloning and characterization of the lanosterol 14α-demethylase gene from Antrodia cinnamomea (2010) J Agr Food Chem, 58, pp. 4800-4807Lees, N.D., Skaggs, B., Kirsch DR and BirdM(1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae - A review Lipids, 30, pp. 221-226Lepesheva, G.I., Waterman, M.R., Sterol 14α-demethylase cytochrome P450 (CYP51), a P450 in all biological kingdoms (2007) Biochim Biophys Acta, 3, pp. 467-477Luo, C.X., Schnabel, G., The cytochrome P450 lanosterol 14α-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilia fructicola field isolates from Georgia (2008) Appl Environ Microb, 74, pp. 359-366Martin, F., Aerts, A., Ahrén, D., Brun, A., Danchin, E.G., Duchaussoy, F., Gibon, J., Pereda, V., The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis (2008) Nature, 452, pp. 88-92McQuilken, M.P., Rudgard, S.A., Sensitivity of Crinipellis periciosa to two triazole fungicides in vitro and their effect on development of the fungus in cocoa (1988) Plant Pathol, 37, pp. 499-506Meinhardt, L.W., Bellato, C.M., Rincones, J., Azevedo, R.A., Cascardo, J.C.M., Pereira, G.A.G., In vitro production of biotrophic- like cultures of Crinipellis perniciosa, the causal agent of Witches’ broom disease of Theobroma cacao (2006) Curr Microbiol, 52, pp. 191-196Mellado, E., Guerra, T.M.D., Estrela, M.C., Tudela, J.L.R., Identification of two different 14α-sterol demethylase related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species (2001) J Clin Microbiol 39:2431-, p. 2438Mondego, J.M.C., Carazzolle, M.F., Costa, G.G.L., Formighieri, E.F., Parizzi, L.P., Rincones, J., Cotomacci, C., Carrer, H., A genome survey of Moniliophthora perniciosa gives new insights into Witches’ broom disease of cacao (2008) BMC Genomics, 9, pp. 1-25Morales, I.J., Vohra, P.K., Puri, V., Kottom, T.J., Limper, A.H., Thomas, C.F., Characterization of a lanosterol 14α demethylase from Pneumocystis carinii (2003) Am J Resp Cell Mol, 29, pp. 232-238Mota, S.G.R., Barros, T.F., Castilho, M.S., In vitro screening and chemometrics analysis on a series of azole derivativeswith fungicide activity against Moniliophthora perniciosa (2010) J Braz Chem Soc, 21, pp. 510-519Nierman, W.C., Pain, A., Anderson, M.J., Wortman, J.R., Kim, H.S., Arroyo, J., Berriman, M., Bermejo, C., Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus (2005) Nature, 438, pp. 1151-1156Page, R.D.M., TREEVIEW: An application to display phylogenetic trees on personal computers (1996) Comput Appl Biosci, 12, pp. 357-358Park, H.G., Lee, I.S., Chun, Y.J., Yun, C.H., Johnston, J.B., Montellano, P.R.O., Kim, D., Heterologous expression and characterization of the sterol 14α-demethylase CYP51F from Candida albicans (2011) Arch Biochem Biophys, 509, pp. 9-15Pereira, J.L., Ram, A., Figueiredo, J.M., Almeida, L.C.C., Primeira ocorrência de vassoura-de-bruxa na principal região produtora de cacau do Brasil (1989) Agrotrópica, 1, pp. 79-81Petersen, T.N., Brunak, S., Heijne, G., Nielsen, H., SignalIP 4.0: Discriminating signal peptides from transmembrane regions (2011) Nat Methods, 10, pp. 785-786Pietila, M.P., Vohra, P.K., Sanyat, B., Wengenack, N.L., Raghavakaimal, S., Thomas, C.F., Cloning and characterization of CYP51 from Mycobacterium avium (2006) Am J Resp Cell Mol, 35, pp. 236-240Pires, A.B.L., Gramacho, K.P., Silva, D.C., Góes-Neto, A., Silva, M.M., Muniz-Sobrinho, J.S., Porto, R.F., Cascardo, J.C.M., Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes (2009) BMC Microbiol, 9, p. e158Purdy, L.H., Schimidt, R.A., Status of cacao witches’ broom: Biology, epidemiology, and management (1996) Annu Rev Phytopathol, 34, pp. 573-594Raeder, U., Broda, P., Rapid preparation of DNA from filamentous fungi (1985) Lett Appl Microbiol, 1, pp. 17-20Revankar, S.G., Fu, J., Rinaldi, M.G., Kelly, S.L., Kelly, D.E., Lamb, D.C., Keller, S.M., Wickes, B.L., Cloning and characterization of the lanosterol 14α-demethylase (ERG11) gene in Cryptococcus neoformans (2004) Biochem Biophys Res Commun, 324, pp. 719-728Rincones, J., Scarpari, L.M., Carazzolle, M.F., Mondego, J.M.C., Formighieri, E.F., Barau, J.G., Costa, G.G.L., Vilas-Boas, L.A., Differential gene expression between the biotrophic-like and saprotrophic mycelia of the witches’ broom pathogen Moniliophthora perniciosa (2008) Mol Plant Microbe Int, 21, pp. 891-908Rio, M.C.S., Oliveira, B.V., Tomazella, D.P.T., Silva, J.A.F., Pereira, G.A.G., Production of calcium oxalate crystals by the basidiomycete Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacao (2008) Curr Microbiol, 56, pp. 363-370Rozman, D., Stromstedt, M., Tsui, L.C., Scherer, S.W., Waterman, M.R., Structure and mapping of the human lanosterol 14α-demethylase gene (CYP51) encoding the cytochrome P450 involved in cholesterol biosynthesis: Comparison of exon/intron organization with other mammalian and fungal CYP genes (1996) Genomics, 38, pp. 371-381Sheng, C., Miao, Z., Ji, H., Yao, J., Wang, W., Che, X., Dong, G., Zhang, W., Three-dimensional model of lanosterol 14α-demethylase from Cryptococcus neoformans: Active- site characterization and insights into azole binding (2009) Antimicrob Agents Chemother, 53, pp. 3487-3495Sigrist, C.J.A., Cerutti, L., Castro, E., Langendijk-Genevaux, P.S., Bulliard, V., Bairoch, A., Hulo, N., PROSITE, a protein domain database for functional characterization and annotation (2009) Nucleic Acids Res, 38, pp. 161-166Stajich, J.E., Wilke, S.K., Ahrén, D., Au, C.H., Birren, B.W., Borodovsky, M., Burns, C., Cheng, C.K., Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus) (2010) Proc Natl Acad Sci USA, 107, pp. 11889-11894Stanke, M., Keller, O., Gunduz, I., Hayes, A., Waack, S., Morgenstern, B., AUGUSTUS: Ab initio prediction of alternative transcripts (2006) Nucleic Acids Res, 34, pp. 435-439Swofford, D.L., PAUP - Phylogenetic Analysis Using Parsimony (and other methods). Version 4.0b10 (2002) Sinauer Associates, , Sunderland, MATer-Hovhannisyan, V., Lomsadze, L., Chernoff, Y.O., Borodovsky, M., Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training (2008) Genome Res, 18, pp. 1979-1990Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weigh matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680Veen, M., Lang, C., Interactions of the ergosterol biosynthetic pathway with other lipid pathways (2005) Biochem Soc Trans, 33, pp. 1178-1181Warrilow, A.G.S., Melo, N., Martel, C.M., Parker, J.E., Nes, W.D., Kelly, S.L., Kelly, D.E., Expression, purification and characterization of Aspergillus fumigatus sterol 14α demethylase (CYP51) isoenzymes A and B (2010) Antimicrob Agents Chemother, 54, pp. 4225-4234Waterman, M.R., Lepesheva, G.I., Sterol 14 _-demethylase, an abundant and essential mixed-function oxidase (2005) Biochem Biophys Res Commun, 338, pp. 418-422Wood, H.M., Dickinson, M.J., Lucas, J.A., Dyer, P.S., Cloning of the CYP51 gene from the eyespot pathogen Tapesia yallundae indicates that resistance to the DMI fungicide prochloraz is not related to sequence changes in the gene encoding the target site enzyme (2001) FEMS Microbiol Lett, 196, pp. 183-187Zhao, L., Liu, D., Zhang, Q., Zhang, S., Wan J and XiaoW(2007) Expression and homology modeling of sterol 14α-demethylase from Penicillium digitatium FEMS Microbiol Lett, 277, pp. 37-4
Distribution and fluctuations of backswimmers (Notonectidae) in a tropical shallow lake and predation on microcrustaceans
Spatial and temporal distribution of gerrid (Heteroptera) and predation on microcrustaceans from a tropical shallow lake
NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics
Xenarthrans – anteaters, sloths, and armadillos – have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with 24 domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, ten anteaters, and six sloths. Our dataset includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data-paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the south of the USA, Mexico, and Caribbean countries at the northern portion of the Neotropics, to its austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n=5,941), and Cyclopes sp. has the fewest (n=240). The armadillo species with the most data is Dasypus novemcinctus (n=11,588), and the least recorded for Calyptophractus retusus (n=33). With regards to sloth species, Bradypus variegatus has the most records (n=962), and Bradypus pygmaeus has the fewest (n=12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other datasets of Neotropical Series which will become available very soon (i.e. Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans dataset
One sixth of Amazonian tree diversity is dependent on river floodplains
Amazonia's floodplain system is the largest and most biodiverse on Earth. Although forests are crucial to the ecological integrity of floodplains, our understanding of their species composition and how this may differ from surrounding forest types is still far too limited, particularly as changing inundation regimes begin to reshape floodplain tree communities and the critical ecosystem functions they underpin. Here we address this gap by taking a spatially explicit look at Amazonia-wide patterns of tree-species turnover and ecological specialization of the region's floodplain forests. We show that the majority of Amazonian tree species can inhabit floodplains, and about a sixth of Amazonian tree diversity is ecologically specialized on floodplains. The degree of specialization in floodplain communities is driven by regional flood patterns, with the most compositionally differentiated floodplain forests located centrally within the fluvial network and contingent on the most extraordinary flood magnitudes regionally. Our results provide a spatially explicit view of ecological specialization of floodplain forest communities and expose the need for whole-basin hydrological integrity to protect the Amazon's tree diversity and its function.Naturali
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time, and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space. While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes, vast areas of the tropics remain understudied. In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity, but it remains among the least known forests in America and is often underrepresented in biodiversity databases. To worsen this situation, human-induced modifications may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge, it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost
La lagune de la Barra (Brésil) : causes et conséquences d'une mortalité de poissons
Le zooplancton d'une lagune côtière brésilienne, la lagune de la Barra (22° 57' S, 42° 47'O), a été suivi hebdomadairement pendant une période de six mois, couvrant l'été et l'automne 1990-91. Durant cette période, l'holoplancton est représenté par des espèces typiquement lagunaires, euryhalines, comme les rotifères #Brachionus plicatilis et #Hexarthra fennica et le copépode #Apocyclops procerus, tandis que le méroplancton est constitué par les larves nauplii de crustacés cirripèdes, véligères de gastéropodes et zoés de décapodes. La période d'étude est marquée par une mortalité de poissons qui a lieu en février 1991 et qui provoque diverses altérations dans l'écosystème. Les densités et biomasses zoopplanctoniques se sont maintenues basses jusqu'à deux semaines après la mortalité (respectivement 55-375 ind/l et 2-5 microg/l), puis elles ont atteint des valeurs élevées (respectivment 2 500-11 000 ind/l et 235-1 450 microg/l) avant de revenir aux valeurs initiales deux mois plus tard. L'importance de #Apocyclops procerus s'est accrue dans la communauté zooplanctonique qui auparavant était dominée par #Brachionus plicatilis. Le développement du zooplancton a été enregistré deux semaines aprés le remplacement des #Cyanophyceae par des #Chlorophyceae, #Bacillariophyceae et #Prasinophyceae$ dans la communauté phytoplanctonique. Ce fait a conduit à mettre en évidence l'importance de l'adéquation des espèces d'algues dominantes sur le développement de zooplancton et, en retour, le contrôle exercé par le broutage sur l'abondance du phytoplancton. (Résumé d'auteur
Avaliação psicométrica em português do indicador de dor crônica de Helsinki em cães com sinais crônicos de osteoartrite
RESUMO Objetivou-se determinar a validade, a confiabilidade e a sensibilidade do indicador de dor crônica de Helsinki (IDCH) em português, além de estabelecer correlação entre a plataforma de pressão em cães com displasia coxofemoral (DCF). O questionário foi preenchido por tutores de 40 animais com DCF e por 16 tutores de cães saudáveis. Os animais com DCF foram tratados com carprofeno 4,4mg/kg (GT= 21) ou placebo (GP= 19), administrados uma vez ao dia, durante quatro semanas. A avaliação consistiu no preenchimento do questionário (IDCH), na escala analógica visual de dor (EAVdor) e na escala analógica visual de locomoção (EAVloc) com duas semanas (A1) e imediatamente antes do tratamento (A2), duas (S2) e quatro (S4) semanas de tratamento e após duas semanas do término do tratamento (S6). A consistência interna dos dados foi considerada excelente (alfa de Cronbach = 0,89). Houve correlação moderada entre o IDCH e a EAVdor e boa entre o IDCH e a EAVloc, porém não houve diferença entre o grupo tratado com carprofeno e placebo no IDCH. Não houve correlação entre os dados da plataforma de pressão e o IDCH. O questionário apresentou validade de constructo, critério e fidedignidade e pode-se empregá-lo em países de língua portuguesa em cães com osteoartrite