241 research outputs found
Heralded single photon sources: a route towards quantum communication technology and photon standards
Single photon counting, based on single photon sources and detectors, is a key ingredient for certain applications aiming at new quantum information technologies. Quantum cryptography, quantum radiometry, distributed quantum computing, as well as adjacent technologies such as biomedical and astronomical imaging, and low power classical communication also rely on single-photon technology. This paper reviews the present status of single photon sources and related counting measurement techniques, based on correlated (or heralded) photons in parametric down-conversion, and their possible impact on the above mentioned technologies, as well as an assessment for photon standards in the future
The intramitochondrial localization of succinate-yellow tetrazolium reductase with the electron microscope
A new tetrazolium salt, yellow tetrazolium, has been used to localise succinatetetrazolium reductase with the electron microscope. As expected, the formazan did not give high contrast in the optical microscope, but localization with the EM was good. The size of the formazan granules was 60–100Å; lead staining was essential to secure good contrast.Facultad de Ciencias Médica
Phonon-induced dephasing of chromium colour centres in diamond
We report on the coherence properties of single photons from chromium-based
colour centres in diamond. We use field-correlation and spectral lineshape
measurements to reveal the interplay between slow spectral wandering and fast
dephasing mechanisms as a function of temperature. We show that the zero-phonon
transition frequency and its linewidth follow a power-law dependence on
temperature indicating that the dominant fast dephasing mechanisms for these
centres are direct electron-phonon coupling and phonon-modulated Coulomb
coupling to nearby impurities. Further, the observed reduction in the quantum
yield for photon emission as a function of temperature is consistent with the
opening of additional nonradiative channels through thermal activation to
higher energy states predominantly and indicates a near-unity quantum
efficiency at 4 K
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Self-assembly of Fmoc-tetrapeptides based on the RGDS cell adhesion motif
Self-assembly in aqueous solution has been investigated for two Fmoc [Fmoc ¼ N-(fluorenyl)-9-methoxycarbonyl] tetrapeptides comprising the RGDS cell adhesion motif from fibronectin or the scrambled sequence GRDS. The hydrophobic Fmoc unit confers amphiphilicity on the molecules, and
introduces aromatic stacking interactions. Circular dichroism and FTIR spectroscopy show that the self-assembly of both peptides at low concentration is dominated by interactions among Fmoc units, although Fmoc-GRDS shows b-sheet features, at lower concentration than Fmoc-RGDS. Fibre X-ray diffraction indicates b-sheet formation by both peptides at sufficiently high concentration. Strong
alignment effects are revealed by linear dichroism experiments for Fmoc-GRDS. Cryo-TEM and smallangle
X-ray scattering (SAXS) reveal that both samples form fibrils with a diameter of approximately 10 nm. Both Fmoc-tetrapeptides form self-supporting hydrogels at sufficiently high concentration. Dynamic shear rheometry enabled measurements of the moduli for the Fmoc-GRDS hydrogel, however syneresis was observed for the Fmoc-RGDS hydrogel which was significantly less stable to shear. Molecular dynamics computer simulations were carried out considering parallel and antiparallel b-sheet configurations of systems containing 7 and 21 molecules of Fmoc-RGDS or Fmoc-GRDS, the results being analyzed in terms of both intermolecular structural parameters and energy contributions
Hybrid mimetic finite-difference and virtual element formulation for coupled poromechanics
We present a hybrid mimetic finite-difference and virtual element formulation
for coupled single-phase poromechanics on unstructured meshes. The key
advantage of the scheme is that it is convergent on complex meshes containing
highly distorted cells with arbitrary shapes. We use a local pressure-jump
stabilization method based on unstructured macro-elements to prevent the
development of spurious pressure modes in incompressible problems approaching
undrained conditions. A scalable linear solution strategy is obtained using a
block-triangular preconditioner designed specifically for the saddle-point
systems arising from the proposed discretization. The accuracy and efficiency
of our approach are demonstrated numerically on two-dimensional benchmark
problems.Comment: 25 pages, 17 figure
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Influence of elastase on alanine-rich peptide hydrogels
The self-assembly of the alanine-rich amphiphilic peptides Lys(Ala)6Lys (KA6K) and Lys(Ala)6Glu (KA6E)with homotelechelic or heterotelechelic charged termini respectively has been investigated in aqueous solution. These peptides contain hexa-alanine sequences designed to serve as substrates for the enzyme elastase. Electrostatic repulsion of the lysine termini in KA6K prevents self-assembly, whereas in contrast KA6E is observed, through electron microscopy, to form tape-like fibrils, which based on X-ray scattering contain layers of thickness equal to the molecular length. The alanine residues enable efficient packing of the side-chains in a beta-sheet structure, as revealed by circular dichroism, FTIR and X-ray diffraction
experiments. In buffer, KA6E is able to form hydrogels at sufficiently high concentration. These were used as substrates for elastase, and enzyme-induced de-gelation was observed due to the disruption of the beta-sheet fibrillar network. We propose that hydrogels of the simple designed amphiphilic peptide KA6E may serve as model substrates for elastase and this could ultimately lead to applications in biomedicine and regenerative medicine
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Tuning chelation by the surfactant-like peptide A6H using predetermined pH values
We examine the self-assembly of a peptide A6H
comprising a hexa-alanine sequence A6 with a histidine (H) “head group”, which chelates Zn2+ cations. We study the self assembly of A6H and binding of Zn2+ ions in ZnCl2 solutions, under acidic and neutral conditions. A6H self-assembles into nanotapes held together by a β-sheet structure in acidic aqueous solutions. By dissolving A6H in acidic ZnCl2 solutions, the carbonyl oxygen atoms in A6H chelate the Zn2+ ions and allow for β-sheet formation at lower concentrations, consequently reducing the onset concentration for nanotape formation. A6H mixed with water or ZnCl2 solutions under neutral conditions produces short sheets or pseudocrystalline tapes, respectively. The imidazole ring of A6H chelates Zn2+ ions in neutral solutions. The internal structure of nanosheets and pseudocrystalline sheets in neutral solutions is similar to the internal structure of A6H nanotapes in acidic solutions. Our results show that it is possible to induce dramatic changes in the self-assembly and chelation sites of A6H by changing the pH of the solution. However, it is likely that the amphiphilic nature of A6H determines the internal structure of the self-assembled aggregates independent from changes in chelation
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High potency of lipid conjugated TLR7 agonist requires nanoparticulate or liposomal formulation
Conjugation of small molecule agonists of Toll-like receptor 7 (TLR7) to proteins, lipids, or polymers is known to modulate potency, and the physical form or formulation of these conjugates is likely to have a major effect on their immunostimulatory activity. Here, we studied the effect of formulation on potency of a 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE) conjugated TLR7 agonist (DOPE-TLR7a) alongside assessing physical form using Dynamic Light Scattering (DLS), Nanosight Particle Tracking (NTA) analysis and Small Angle X-ray Scattering (SAXS). A very high potency of DOPE-TLR7a conjugate (EC50 around 9 nM) was observed either when prepared by direct dilution from DMSO or when formulated into 400-700 nm large multilamella liposomes containing dimethyldioctadecylammonium bromide salt (DDA) and DOPE. When prepared by dissolution in DMSO followed by dilution in aqueous culture medium, 93 ± 5 nm nanoparticles were formed. Without dilution from solution in DMSO, no nanoparticles were observed, and no immunostimulatory activity could be detected without this formulation step. SAXS analysis of the conjugate after DMSO dissolution/water dilution revealed a lamellar order with a layer spacing of 68.7 Å, which correlates with arrangement in groups of 3 bilayers. The addition of another immunostimulatory glycolipid, trehalose-6,6-dibehenate (TDB), to DOPE:DDA liposomes gave no further increase in immunostimulatory activity beyond that provided by incorporating DOPE-TLR7a. Given the importance of nanoparticle or liposomal formulation for activity, we conclude a major mechanism for increase in potency when TLR7 agonists are conjugated to macromolecules is through alteration of physical form
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