THE ROUTE OF ENTERIC INFECTION IN NORMAL MICE

This study followed the early pathogenesis of orally induced murine typhoid fever. Intragastrically administered Salmonella enteritidis moves quickly through the normal undisturbed gut so that only a small residuum remains in the cecum and large intestine after the first few hours. Dye injection of the gut wall was used to show that lymph from discrete portions of the gastrointestinal tract drains to separate lymph nodes, probably via the regional Peyer's patches. Plating techniques capable of detecting a single colony-forming unit of S. enteritidis within the different Peyer's patches and draining lymph nodes indicate that, although the cecum and large intestine are exposed to large numbers of Salmonella for longer time periods than the small intestine, the primary site of bacterial penetration involves the distal ileum. This area of the small intestine as well as the cecum are both drained by the distal mesenteric lymph nodes, and were the only nodes which contained detectable numbers of viable Salmonella over the first 24 h of infection. Neither the pyloric nor the proximal mesenteric lymph nodes (which drain the stomach and duodenum) nor the pancreatic and caudal lymph nodes (which drain the transverse and descending colon) contained viable Salmonella. Salmonella were observed to infect the ileal mucosa and its Peyer's patches. With time, this infection progresses to the draining lymph node and ultimately reaches the liver and spleen. Some of the implications of these findings relative to the development of acquired resistance to enteric disease are discussed

Initial Effects of Prescribed Burning on Survival and Nesting Success of Northern Bobwhites in West-Central Texas

Fire is often prescribed for managing habitat for northern bobwhites (Colinus virginianus) in the southeastern United States, yet little is known about its use as a tool in more xeric portions of the species’ range. This study was conducted from 1994 to 1995 on 3 sites in the northern Edwards Plateau ecoregion of Texas to monitor immediate post-burn effects on bobwhite ecology. Each site included a burned pasture paired with an unburned control. We radiomarked \u3e= 50 bobwhites (25/pasture) at each study site with neck-loop transmitters just prior to burning and monitored their survival and nesting habits for 6 months post-burn. Survival was similar (P \u3e 0.05) between burned and unburned areas. Predation was the leading cause of mortality, with mammals and raptors accounting for 68% and 31% of the predation, respectively. Nest initiation and success were low for both treatments. Nest sites occurred mostly in association with prickly pear cactus (Opuntia spp.). Our results suggest that relatively ‘‘cool’’ prescribed burns had few short-term effects on bobwhite survival in west-central Texas. However, reductions in cacti density and cover that often occur post-burn, especially if followed by an application of herbicide (i.e., picloram), may reduce the number of potential nesting sites for bobwhites

Intrinsic and extrinsic factors drive ontogeny of early-life at-sea behaviour in a marine top predator

Young animals must learn to forage effectively to survive the transition from parental provisioning to independent feeding. Rapid development of successful foraging strategies is particularly important for capital breeders that do not receive parental guidance after weaning. The intrinsic and extrinsic drivers of variation in ontogeny of foraging are poorly understood for many species. Grey seals (Halichoerus grypus) are typical capital breeders; pups are abandoned on the natal site after a brief suckling phase, and must develop foraging skills without external input. We collected location and dive data from recently-weaned grey seal pups from two regions of the United Kingdom (the North Sea and the Celtic and Irish Seas) using animal-borne telemetry devices during their first months of independence at sea. Dive duration, depth, bottom time, and benthic diving increased over the first 40 days. The shape and magnitude of changes differed between regions. Females consistently had longer bottom times, and in the Celtic and Irish Seas they used shallower water than males. Regional sex differences suggest that extrinsic factors, such as water depth, contribute to behavioural sexual segregation. We recommend that conservation strategies consider movements of young naïve animals in addition to those of adults to account for developmental behavioural changes

Explaining the variability of WD 1145+017 with simulations of asteroid tidal disruption

Post-main-sequence planetary science has been galvanised by the striking variability, depth and shape of the photometric transit curves due to objects orbiting white dwarf WD 1145+017, a star which also hosts a dusty debris disc and circumstellar gas, and displays strong metal atmospheric pollution. However, the physical properties of the likely asteroid which is discharging disintegrating fragments remain largely unconstrained from the observations. This process has not yet been modelled numerically. Here, we use the N-body code PKDGRAV to compute dissipation properties for asteroids of different spins, densities, masses, and eccentricities. We simulate both homogeneous and differentiated asteroids, for up to two years, and find that the disruption timescale is strongly dependent on density and eccentricity, but weakly dependent on mass and spin. We find that primarily rocky differentiated bodies with moderate (∼ 3−4 g/cm3 ) bulk densities on near-circular (e . 0.1) orbits can remain intact while occasionally shedding mass from their mantles. These results suggest that the asteroid orbiting WD 1145+017 is differentiated, resides just outside of the Roche radius for bulk density but just inside the Roche radius for mantle density, and is more akin physically to an asteroid like Vesta instead of one like Itokawa

Cluster Alignments and Ellipticities in LCDM Cosmology

The ellipticities and alignments of clusters of galaxies, and their evolution with redshift, are examined in the context of a Lambda-dominated cold dark matter cosmology. We use a large-scale, high-resolution N-body simulation to model the matter distribution in a light cone containing ~10^6 clusters out to redshifts of z=3. Cluster ellipticities are determined as a function of mass, radius, and redshift, both in 3D and in projection. We find strong cluster ellipticities: the mean ellipticity increases with redshift from 0.3 at z=0 to 0.5 at z=3, for both 3D and 2D ellipticities; the evolution is well-fit by e=0.33+0.05z. The ellipticities increase with cluster mass and with cluster radius; the main cluster body is more elliptical than the cluster cores, but the increase of ellipticities with redshift is preserved. Using the fitted cluster ellipsoids, we determine the alignment of clusters as a function of their separation. We find strong alignment of clusters for separations <100 Mpc/h; the alignment increases with decreasing separation and with increasing redshift. The evolution of clusters from highly aligned and elongated systems at early times to lower alignment and elongation at present reflects the hierarchical and filamentary nature of structure formation. These measures of cluster ellipticity and alignment will provide a new test of the current cosmological model when compared with upcoming cluster surveys.Comment: 29 pages including 13 figures, to appear in ApJ Jan. 2005 (corrected typos, added reference

Navigating uncertain waters:a critical review of inferring foraging behaviour from location and dive data in pinnipeds

In the last thirty years, the emergence and progression of biologging technology has led to great advances in marine predator ecology. Large databases of location and dive observations from biologging devices have been compiled for an increasing number of diving predator species (such as pinnipeds, sea turtles, seabirds and cetaceans), enabling complex questions about animal activity budgets and habitat use to be addressed. Central to answering these questions is our ability to correctly identify and quantify the frequency of essential behaviours, such as foraging. Despite technological advances that have increased the quality and resolution of location and dive data, accurately interpreting behaviour from such data remains a challenge, and analytical methods are only beginning to unlock the full potential of existing datasets. This review evaluates both traditional and emerging methods and presents a starting platform of options for future studies of marine predator foraging ecology, particularly from location and two-dimensional (time-depth) dive data. We outline the different devices and data types available, discuss the limitations and advantages of commonly-used analytical techniques, and highlight key areas for future research. We focus our review on pinnipeds - one of the most studied taxa of marine predators - but offer insights that will be applicable to other air-breathing marine predator tracking studies. We highlight that traditionally-used methods for inferring foraging from location and dive data, such as first-passage time and dive shape analysis, have important caveats and limitations depending on the nature of the data and the research question. We suggest that more holistic statistical techniques, such as state-space models, which can synthesise multiple track, dive and environmental metrics whilst simultaneously accounting for measurement error, offer more robust alternatives. Finally, we identify a need for more research to elucidate the role of physical oceanography, device effects, study animal selection, and developmental stages in predator behaviour and data interpretation

Remotely sensed and modelled pasture biomass, land condition and the potential to improve grazing-management decision tools across the Australian rangelands

This report assesses the potential for expanding on current capacity to monitor land condition using remotely sensed fractional cover products to improve biomass estimation, animal productivity, pasture growth models and grazing decision tools (e.g. safe carrying capacity) across the Australian rangelands. We focus on northern Australia and include relevant research and implementation from southern Australia where appropriate

UBC-Nepal expedition: The use of oral antioxidants does not alter cerebrovascular function at sea-level or high-altitude

Hypoxia is associated with an increased systemic and cerebral formation of free radicals and associated reactants that may be linked to impaired cerebral vascular function a neurological sequela. To what extent oral antioxidants prophylaxis impacts cerebrovascular function in humans throughout the course of acclimatization to the hypoxia of terrestrial high-altitude has not been examined. Thus, the purpose of the current study was to examine the influence of orally ingested antioxidants at clinically relevant doses (vitamin C, E, and alpha-lipoic acid) on cerebrovascular regulation at sea-level (344 m; n = 12; female n = 2 participants), and at high altitude (5050 m; n = 9; female n = 2), in a randomized, placebo-controlled, and double-blinded crossover design. Hypercapnic and hypoxic cerebrovascular reactivity tests of the internal carotid (ICA)] were conducted at sea-level, while global and regional cerebral blood flow [i.e. ICA and vertebral artery (VA)] were assessed after 10–12 days following arrival at 5050 m. At sea-level, acute administration of antioxidants did not alter cerebral hypoxic cerebrovascular reactivity (pre vs. post: 1.5 ± 0.7 vs. 1.2 ± 0.8 %∆CBF/-%∆SpO2; P = 0.96), or cerebral hypercapnic cerebrovascular reactivity (pre vs. post: 5.7 ± 2.0 vs. 5.8 ± 1.9 %∆CBF/∆mmHg; P = 0.33). Furthermore, global cerebral blood flow (P = 0.43), as well as cerebral vascular conductance (ICA P = 0.08; VA P = 0.32), were unaltered at 5050 m following antioxidant administration. In conclusion, these data show that an oral antioxidant cocktail known to attenuate systemic oxidative stress failed to alter cerebrovascular function at sea-level and cerebral blood flow during acclimatization to high-altitude