Immersion Pulmonary Edema in Female Triathletes

Pulmonary edema has been reported in SCUBA divers, apnea divers, and long-distance swimmers however, no instances of pulmonary edema in triathletes exist in the scientific literature. Pulmonary edema may cause seizures and loss of consciousness which in a water environment may become life threatening. This paper describes pulmonary edema in three female triathletes. Signs and symptoms including cough, fatigue, dyspnea, haemoptysis, and rales may occur within minutes of immersion. Contributing factors include hemodynamic changes due to water immersion, cold exposure, and exertion which elevate cardiac output, causing pulmonary capillary stress failure, resulting in extravasation of fluid into the airspace of the lung. Previous history is a major risk factor. Treatment involves immediate removal from immersion and in more serious cases, hospitalization, and oxygen administration. Immersion pulmonary edema is a critical environmental illness of which triathletes, race organizers, and medical staff, should be made aware

FGFR2 amplification in colorectal adenocarcinoma

FGFR2 is recurrently amplified in 5% of gastric cancers and 1%–4% of breast cancers; however, this molecular alteration has never been reported in a primary colorectal cancer specimen. Preclinical studies indicate that several FGFR tyrosine-kinase inhibitors (TKIs), such as AZD4547, have in vitro activity against the FGFR2-amplified colorectal cell line, NCI-H716. The efficacy of these inhibitors is currently under investigation in clinical trials for breast and gastric cancer. Thus, better characterizing colorectal tumors for FGFR2 amplification could identify a subset of patients who may benefit from FGFR TKI therapies. Here, we describe a novel FGFR2 amplification identified by clinical next-generation sequencing in a primary colorectal cancer. Further characterization of the tumor by immunohistochemistry showed neuroendocrine differentiation, similar to the reported properties of the NCI-H716 cell line. These findings demonstrate that the spectrum of potentially clinically actionable mutations detected by targeted clinical sequencing panels is not limited to only single-nucleotide polymorphisms and insertions/deletions but also to copy-number alterations.</jats:p

Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells

Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells. Jasmine D. Carter1, Giovanni Reyes1, Abeeha Choudhary2 and Eric A. Albrecht1 Breast cancer is known for its diverse clinical classifications and expressing different levels of membrane proteins such as ion channels and gap junctions. This diversity allows more variations in cell polarization, which can lead to enhanced directional ion fluxes in certain breast cancer subtypes. We utilized the interactive web portal UALCAN to evaluate the gene expression data of gap junctions, ion exchange channels and cytoskeletal proteins in breast cancer tissues. Our data showed several gene targets (e.g., GJA1(Cx43),GJB2(Cx26) increased expression during tumor development compared to normal breast tissue. Immunocytochemistry protocols were developed to examine the spatial expression of GJB2(Cx26) in MCF10A (normal breast cells) and MCF7 (weakly metastatic) breast cancer cell lines under static conditions. Primary antibodies to GJB2(Cx26) were visualized using fluorescein conjugated anti-mouse IgG (H+L) secondary antibodies. Our data suggests that the use of genomic and proteomic expression data is an effective approach for identifying differential expression differences in normal and malignant tissues. 1Kennesaw State University, Department of Molecular and Cellular Biology, Kennesaw, GA 30144 2Kennesaw State University, Department of Psychological Science, Kennesaw, GA 3014

Multiple Captures of White-footed Mice (Peromyscus leucopus): Evidence for Social Structure?

Multiple captures (34 double, 6 triple) in standard Sherman live traps accounted for 6.3% of 1355 captures of Peromyscus leucopus (white-footed mice) in forested habitat in southern Illinois, from Oct. 2004 through Oct. 2005. There was a significant positive relationship between both the number and the proportion of multiple captures and estimated monthly population size. Multiple captures were all intraspecific and occurred significantly more often from Nov. through Mar. when animals were not reproductively active, but this was confounded by seasonal changes in abundance. Age/gender distribution of animals in double captures did not differ from that expected from random pairing. We suggest that sociality and synchronous entry of two white-footed mice into traps were the primary determinants of double captures; sensitivity of traps may have been the primary factor in triple capture

Infrared study of the eta Chamaeleontis cluster and the longevity of circumstellar discs

We have analyzed JHKL observations of the stellar population of the ~9 Myr-old eta Chamaeleontis cluster. Using infrared (IR) colour-colour and colour-excess diagrams, we find the fraction of stellar systems with near-IR excess emission is 0.60 pm 0.13 (2_sigma). This results implies considerably longer disc lifetimes than found in some recent studies of other young stellar clusters. For the classical T Tauri (CTT) and weak-lined T Tauri (WTT) star population, we also find a strong correlation between the IR excess and H_alpha emission. The IR excesses of these stars indicate a wide range of star-disc activity; from a CTT star showing high levels of accretion, to CTT - WTT transition objects with evidence for some on-going accretion, and WTT stars with weak or absent IR excesses. Of the 15 known cluster members, 4 stars with IR excesses delta(K-L) > 0.4 mag are likely experiencing on-going accretion owing to strong or variable optical emission. The resulting accretion fraction (0.27 pm 0.13; 2_sigma) shows that the accretion phase, in addition to the discs themselves, can endure for at least ~10 Myr.Comment: 7 pages, 4 figures, accepted for MNRA

Pathogenicity locus, core genome, and accessory gene contributions to Clostridium difficile virulence

Clostridium difficile is a spore-forming anaerobic bacterium that causes colitis in patients with disrupted colonic microbiota. While some individuals are asymptomatic C. difficile carriers, symptomatic disease ranges from mild diarrhea to potentially lethal toxic megacolon. The wide disease spectrum has been attributed to the infected host’s age, underlying diseases, immune status, and microbiome composition. However, strain-specific differences in C. difficile virulence have also been implicated in determining colitis severity. Because patients infected with C. difficile are unique in terms of medical history, microbiome composition, and immune competence, determining the relative contribution of C. difficile virulence to disease severity has been challenging, and conclusions regarding the virulence of specific strains have been inconsistent. To address this, we used a mouse model to test 33 clinical C. difficile strains isolated from patients with disease severities ranging from asymptomatic carriage to severe colitis, and we determined their relative in vivo virulence in genetically identical, antibiotic-pretreated mice. We found that murine infections with C. difficile clade 2 strains (including multilocus sequence type 1/ribotype 027) were associated with higher lethality and that C. difficile strains associated with greater human disease severity caused more severe disease in mice. While toxin production was not strongly correlated with in vivo colonic pathology, the ability of C. difficile strains to grow in the presence of secondary bile acids was associated with greater disease severity. Whole-genome sequencing and identification of core and accessory genes identified a subset of accessory genes that distinguish high-virulence from lower-virulence C. difficile strains

Donning a Novel Lower-Limb Restrictive Compression Garment During Training Augments Muscle Power and Strength

International Journal of Exercise Science 13(3): 890-899, 2020. The popularity of graduated compression garments (GCG) in sport and exercise is largely driven by the abundance of anecdotal claims suggesting their efficacy. A new line of compression apparel, restrictive compression garments (RCG), integrate novel resistance technology into lower-limb compression garments designed to provide variable resistance to movement. This study aimed to investigate the effect of donning an RCG during a 4-week training program on selected performance variables. Twelve college-aged males were recruited for four weeks of lower-body strength-power resistance training. Participants were randomized 1:1 and blinded to (i) an intervention group (RCG; n = 6) that donned a lower-body RCG during training or (ii) a control group (SHAM; n = 6) that donned a sham during identical training. Both groups demonstrated significant increases in 1-repetition maximum (1-RM) on a seated leg press after 4 weeks (both p \u3c 0.001), with RCG showing a significantly greater increase compared SHAM (p = 0.005, g = 3.35). Similarly, RCG demonstrated significantly greater increases in jump height, peak power, and average power compared to SHAM (p = 0.032, g = 3.44; p \u3c 0.001, g = 4.40; p \u3c 0.001, g = 4.50, respectively). Donning a RCG while engaging in lower-body strength-power training may augment increases 1-RM on a seated leg press, jump height, peak and average power, compared with same exercise training without an RCG