Public space planning in minor historic centres exposed to seismic risk: lessons learnt from the experience in Navelli (AQ)

Risk reduction in minor historic centres exposed to seismic hazards is essential for the protection of life and cultural heritage but also for social and economic development and requires appropriate strategies. The current state of knowledge and technology suggests that intervention on sensitive mitigation of urban systems vulnerability is the most desirable solution to prevent the devastating earthquake’s effects. This requires a careful planning of both built and public spaces. Within this framework, the contribution illustrates an integrated methodology that accompanied the drafting of the Reconstruction plan of Navelli (AQ) and Civitaretenga, drawn up in response to the earthquake that struck the Abruzzo Region in 2009. Although dated, this methodology can be considered a best practice due to the innovative systematic assessment of both built heritage and open space in the two historical centres, supported by an Integrated Information System (IIS). An innovative approach to the assessment of vulnerability and accessibility of public spaces is also introduced. Monitoring the first outcomes of the Plan implementation provides a pretext for a critical reflection, about 10 years later, on the role of post-earthquake planning tools and on the evident relations or gaps between the scientific and technical contribution of the university and ordinary reconstruction processes in minor urban centres, generally prioritising interventions on the built heritage over the public space

Exploring the 15-minute neighbourhoods. An evaluation based on the walkability performance to public facilities

Recent trends in urban policies are rediscovering a new focus on different urban life models that exclude the obsolete vehicle model and lead to renewed attention on the proximity dimension and active mobility. This vision takes concrete form in the concept of the 15-minute city model, focused on pedestrian accessibility from one’s home to nearby urban services and spaces. In this perspective, the paper aims at exploring the theme of 15-minute cities through a GIS-based model to evaluate pedestrian accessibility to neighbourhood facilities. The implemented methodology integrates the assessment of walking distances, considering the time factor as crucial, and mapping the resident population. The method is then applied to measure the current performances of an existing neighbourhood in Parma from the 15 minutes city perspective, assessing accessibility based on home-facility travel times and the resident population distribution within reach. A reflection is proposed on what has been learned and on the possible contribution that the method can bring to monitoring the 15-minute city and to urban planning

Widespread Urban Regeneration of Existing Residential Areas in European Medium-Sized Cities. A Framework to Locate Redevelopment Interventions

Within the scientific debate on urban regeneration, this paper intends to question possible planning techniques to address the implementation of widespread regeneration interventions in obsolete residential areas within medium-sized European cities, with particular attention to constructing a cognitive framework to locate redevelopment interventions. The widespread urban regeneration approach has yet to be sufficiently explored in the scientific literature, which focuses much more on replacing large derelict areas, though it seems particularly relevant for research and urban practice. This paper aims to illustrate a methodological framework for defining obsolete and degraded areas (at the block scale) suitable for redevelopment. Various criteria are considered, e.g., land use, buildings’ dating, state of preservation, population density, public spaces, and facility provision, and degree of accessibility. The methodological framework is then tested in the medium-sized Italian city of Parma. Data set parameters and threshold values to quantify the previously introduced criteria and perform GIS-based statistical and spatial analysis. The results show 96 areas potentially in need of regeneration, providing an opportunity to reflect on the criteria of suitability and priority for transformation and the framework of past and future planning scenarios

From urban planning techniques to 15-minute neighbourhoods. A theoretical framework and GIS-based analysis of pedestrian accessibility to public services

The enthusiasm with which several metropolises have promoted and embraced the idea of the '15-minute city' may now provide a useful opportunity to reflect once again on the well-known relationship between urban planning and mobility planning. Urban policies and plans are recently rediscovering a new focus on the proximity dimension and active mobility, progressively excluding the obsolete car-centric model. In this perspective, the paper aims at assessing 15-minute neighbourhoods through a GIS-based model to evaluate pedestrian accessibility to urban services and spaces. The implemented methodology integrates the assessment of walking distances and the mapping of the resident population distribution. The method is then applied to measure the current performance of an existing neighbourhood in Parma, assessing accessibility based on home-facility travel times and the resident population within reach. A reflection is proposed on what has been learned and on the possible contribution that the method can bring to urban monitoring and planning

Urban Development and Airports in Northern and Central Italy: Main Trends and a Focus on Parma Giuseppe Verdi Case Study

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Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development

<p>Abstract</p> <p>Background</p> <p><it>Prox1</it>, the vertebrate homolog of <it>prospero </it>in <it>Drosophila melanogaster</it>, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development.</p> <p>Results</p> <p>Here we report that, in zebrafish, <it>prox1 </it>is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced <it>prox1 </it>expression in a group of neurons, already positive for <it>otp1</it>, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic <it>prox1/otp1 </it>overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium.</p> <p>Conclusion</p> <p>Our findings indicate that <it>prox1 </it>activity is crucial for the proper development of the <it>otp1</it>-positive hypothalamic neuronal precursors to their terminal CA phenotype.</p

Carcinoid Syndrome. Preclinical Models and Future Therapeutic Strategies

Carcinoid syndrome represents a debilitating paraneoplastic disease, caused by the se- cretion of several substances, occurring in about 10–40% of patients with well-differentiated neu- roendocrine tumors (NETs). The main signs and symptoms associated with carcinoid syndrome are flushing, diarrhea, hypotension, tachycardia, bronchoconstriction, venous telangiectasia, dyspnea and fibrotic complications (mesenteric and retroperitoneal fibrosis, and carcinoid heart disease). Although there are several drugs available for the treatment of carcinoid syndrome, the lack of therapeutic response, poor tolerance or resistance to drugs are often reported. Preclinical models are indispensable tools for investigating the pathogenesis, mechanisms for tumor progression and new therapeutic approaches for cancer. This paper provides a state-of-the-art overview of in vitro and in vivo models in NETs with carcinoid syndrome, highlighting the future developments and therapeutic approaches in this field

Risks in Loading and Unloading LPG at Storage Sites: Analysis of a set of Quasi-accidental Events

In Europe, several industrial plants present significant quantities of dangerous substances that can potentially cause major accidents with catastrophic consequences and domino effects on the surrounding areas. LPG storage sites belong to that category of installations and they are subject to the Directive 2012/18/EU. In such high-risk contexts, near-misses associated to LPG transfer and handling can represent a possible precursor of catastrophic events in the long term, therefore suggesting corrective and improvement actions. In the present study, the sequence of quasi-accidental events that occurred between 2012 and 2021 in an Italian LPG storage site, whose main activity consists of receiving, storing, bottling and shipping LPG, is analysed. Data are extracted from the company accidents database in the form of operative experience sheets for Seveso audits, as well as from other internal technical documents. The focus is on LPG transfer operations and the multiple factors (technical, organizational and human ones) that may have contributed to the occurrence of the undesired events, with a particular attention to maintenance procedures and machinery management. Results are expected to contribute to the understanding of the relationships between precursor events and possible major accidents in critical industrial sites

Modeling Lung Carcinoids with Zebrafish Tumor Xenograft

Lung carcinoids are neuroendocrine tumors that comprise well-differentiated typical (TCs) and atypical carcinoids (ACs). Preclinical models are indispensable for cancer drug screening since current therapies for advanced carcinoids are not curative. We aimed to develop a novel in vivo model of lung carcinoids based on the xenograft of lung TC (NCI-H835, UMC-11, and NCI-H727) and AC (NCI-H720) cell lines and patient-derived cell cultures in Tg(fli1a:EGFP)(y1) zebrafish embryos. We exploited this platform to test the anti-tumor activity of sulfatinib. The tumorigenic potential of TC and AC implanted cells was evaluated by the quantification of tumor-induced angiogenesis and tumor cell migration as early as 24 h post-injection (hpi). The characterization of tumor-induced angiogenesis was performed in vivo and in real time, coupling the tumor xenograft with selective plane illumination microscopy on implanted zebrafish embryos. TC-implanted cells displayed a higher pro-angiogenic potential compared to AC cells, which inversely showed a relevant migratory behavior within 48 hpi. Sulfatinib inhibited tumor-induced angiogenesis, without affecting tumor cell spread in both TC and AC implanted embryos. In conclusion, zebrafish embryos implanted with TC and AC cells faithfully recapitulate the tumor behavior of human lung carcinoids and appear to be a promising platform for drug screening

Foodborne Salmonellosis in Italy: Characterization of Salmonella enterica Serovar Typhimurium and Monophasic Variant 4,[5],12:i- Isolated from Salami and Human Patients.

Salmonella enterica serovar Typhimurium (STm) and its monophasic variant 4,[5],12:i:- (VMSTm) have been responsible for an increased number of foodborne infections in humans in Europe in recent years. The aim of this study was to investigate the origin of three foodborne salmonellosis outbreaks that occurred in Pavia Province (Lombardy region, northern Italy) in 2010. Phenotypic and genetic characteristics of the STm and VMSTm isolates from patients and from food that were recovered in the framework of the three outbreaks were evaluated through serotyping, phage typing, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and multiple-locus variable-number tandem repeat analysis (MLVA). Salami from three artisan producers, which had all purchased meat from the same slaughterhouse, was the food source of infection in outbreak I. STm isolates were recovered from salami and patients with symptoms of gastroenteritis. These isolates had the same PFGE type and the same rare MLVA profile (3-18-9-NA-211). The same molecular profiles were found in an STm isolate from a salami, which likely was the source of another family outbreak (II). A VMSTm strain with common phenotypic and molecular profiles was isolated from three hospitalized patients and identified as the cause of another putative outbreak (III). During the following 3 years (2011 through 2013), 360 salami produced in Pavia Province were monitored for the presence of S. enterica . In 2011, no STm and VMSTm isolates were recovered from 159 salami tested. During 2012 and 2013, 13.9% of 201 tested salami harbored S. enterica , and half of the isolates were VMSTm, mainly in salami from those artisan producers involved in the previous outbreaks. These isolates were genetically variable, especially in terms of MLVA profiles. The data collected suggest that from 2012, VMSTm has replaced STm in the environments of the salami producers monitored in this study, and these data confirm the dominance of this emergent serovar along the pork supply chain