83 research outputs found
Pneumococcal Colonisation Rates in Patients Admitted to a UK Hospital with Lower Respiratory Tract Infection - a prospective case-control study
BACKGROUND
Current diagnostic tests are ineffective at identifying the aetiological pathogen in hospitalised adults with lower respiratory tract infection (LRTI). The association of pneumococcal colonisation with disease has been suggested as a means to increase diagnostic precision. We compared pneumococcal colonisation rate and density of nasal pneumococcal colonisation by a) classical culture and b) quantitative real time lytA Polymerase Chain Reaction (qPCR) in patients admitted to hospital in the UK with LRTI compared to control patients.
METHODS
826 patients were screened for inclusion in this prospective case-control study. 38 patients were recruited, 19 with confirmed LRTI and 19 controls with another diagnosis. Nasal wash (NW) was collected at the time of recruitment.
RESULTS
Pneumococcal colonisation was detected in 1 LRTI patient and 3 controls (p=0.6) by classical culture. Using qPCR pneumococcal colonisation was detected in 10 LRTI patients and 8 controls (p=0.5). Antibiotic usage prior to sampling was significantly higher in the LRTI than control group 19 v. 3 (p8000 copies/ml on qPCR pneumococcal colonisation was found in 3 LRTI patients and 4 controls (p > 0.05).
CONCLUSIONS
We conclude that neither prevalence nor density of nasal pneumococcal colonisation (by culture and qPCR) can be used as a method of microbiological diagnosis in hospitalised adults with LRTI in the UK. A community based study recruiting patients prior to antibiotic therapy may be a useful future step
The properties of extragalactic radio sources selected at 20 GHz
We present some first results on the variability, polarization and general
properties of radio sources selected in a blind survey at 20 GHz, the highest
frequency at which a sensitive radio survey has been carried out over a large
area of sky. Sources with flux densities above 100 mJy in the AT20G Pilot
Survey at declination -60 to -70 were observed at up to three epochs during
2002-4, including near-simultaneous measurements at 5, 8 and 18 GHz in 2003. Of
the 173 sources detected, 65% are candidate QSOs, BL Lac objects or blazars,
20% galaxies and 15% faint (b > 22 mag) optical objects or blank fields.
On a 1-2 year timescale, the general level of variability at 20 GHz appears
to be low. For the 108 sources with good-quality measurements in both 2003 and
2004, the median variability index at 20 GHz was 6.9% and only five sources
varied by more than 30% in flux density.
Most sources in our sample show low levels of linear polarization (typically
1-5%), with a median fractional polarization of 2.3% at 20 GHz. There is a
trend for fainter sources to show higher fractional polarization.
At least 40% of sources selected at 20GHz have strong spectral curvature over
the frequency range 1-20 GHz. We use a radio `two-colour diagram' to
characterize the radio spectra of our sample, and confirm that the radio-source
population at 20 GHz (which is also the foreground point-source population for
CMB anisotropy experiments like WMAP and Planck) cannot be reliably predicted
by extrapolating the results of surveys at lower frequencies. As a result,
direct selection at 20 GHz appears to be a more efficient way of identifying 90
GHz phase calibrators for ALMA than the currently-proposed technique of
extrapolation from all-sky surveys at 1-5 GHz.Comment: 14-page paper plus 5-page data table. Replaced with published versio
Pneumococcal colonisation is an asymptomatic event in healthy adults using an experimental human colonisation model
Introduction
Pneumococcal colonisation is regarded as a pre-requisite for developing pneumococcal disease. In children previous studies have reported pneumococcal colonisation to be a symptomatic event and described a relationship between symptom severity/frequency and colonisation density. The evidence for this in adults is lacking in the literature. This study uses the experimental human pneumococcal challenge (EHPC) model to explore whether pneumococcal colonisation is a symptomatic event in healthy adults.
Methods
Healthy participants aged 18–50 were recruited and inoculated intra-nasally with either Streptococcus pneumoniae (serotypes 6B, 23F) or saline as a control. Respiratory viral swabs were obtained prior to inoculation. Nasal and non-nasal symptoms were then assessed using a modified Likert score between 1 (no symptoms) to 7 (cannot function). The rate of symptoms reported between the two groups was compared and a correlation analysis performed.
Results
Data from 54 participants were analysed. 46 were inoculated with S. pneumoniae (29 with serotype 6B, 17 with serotype 23F) and 8 received saline (control). In total, 14 became experimentally colonised (30.4%), all of which were inoculated with serotype 6B. There was no statistically significant difference in nasal (p = 0.45) or non-nasal symptoms (p = 0.28) between the inoculation group and the control group. In those who were colonised there was no direct correlation between colonisation density and symptom severity. In the 22% (12/52) who were co-colonised, with pneumococcus and respiratory viruses, there was no statistical difference in either nasal or non-nasal symptoms (virus positive p = 0.74 and virus negative p = 1.0).
Conclusion
Pneumococcal colonisation using the EHPC model is asymptomatic in healthy adults, regardless of pneumococcal density or viral co-colonisation
The Australia Telescope 20GHz (AT20G) Survey: analysis of the extragalactic source sample
The Australia Telescope 20 GHz (AT20G) survey is a blind survey of the whole
Southern sky at 20 GHz with follow-up observations at 4.8, 8.6, and 20 GHz
carried out with the Australia Telescope Compact Array (ATCA). In this paper we
present an analysis of radio spectral properties in total intensity and
polarisation, sizes, optical identifications, and redshifts of the sample of
the 5808 extragalactic sources in the survey catalogue of confirmed sources
over the whole Southern sky excluding the strip at Galactic latitude
|b|<1.5deg. The sample has a flux density limit of 40 mJy. Completeness has
been measured as a function of scan region and flux density. Averaging over the
whole survey area the follow-up survey is 78% complete above 50mJy and 93%
complete above 100mJy. 3332 sources with declination <-15deg have good quality
almost simultaneous observations at 4.8, 8.6, and 20GHz. The spectral analysis
shows that the sample is dominated by flat-spectrum sources. The fraction of
flat-spectrum sources decreases from 81% for 20GHz flux densities S>500mJy, to
60% for S<100mJy. There is also a clear spectral steepening at higher
frequencies with the median spectral index decreasing from -0.16 between 4.8
and 8.6GHz to -0.28 between 8.6 and 20GHz. Simultaneous observations in
polarisation are available for all the sources at all the frequencies. 768
sources have a good quality detection of polarised flux density at 20GHz; 467
of them were also detected in polarisation at 4.8 and/or at 8.6GHz so that it
has been possible to compare the spectral behaviour in total intensity and
polarisation. We have found that the polarised fraction increases slightly with
frequency and decreases with flux density. Cross matches and comparisons have
been made with other catalogues at lower radio frequencies, and in the optical,
X-ray and gamma-ray bands. Redshift estimates are available for 825 sources.Comment: 15 pages, 16 figures, accepted for publication in MNRA
The Australia Telescope 20 GHz (AT20G) Survey: The Bright Source Sample
The Australia Telescope 20 GHz (AT20G) Survey is a blind survey of the whole
Southern sky at 20 GHz (with follow-up observations at 4.8 and 8.6 GHz) carried
out with the Australia Telescope Compact Array (ATCA) from 2004 to 2007. The
Bright Source Sample (BSS) is a complete flux-limited subsample of the AT20G
Survey catalogue comprising 320 extragalactic (|b|>1.5 deg) radio sources south
of dec = -15 deg with S(20 GHz) > 0.50 Jy. Of these, 218 have near simultaneous
observations at 8 and 5 GHz. In this paper we present an analysis of radio
spectral properties in total intensity and polarisation, size, optical
identifications and redshift distribution of the BSS sources. The analysis of
the spectral behaviour shows spectral curvature in most sources with spectral
steepening that increases at higher frequencies (the median spectral index
\alpha, assuming S\propto \nu^\alpha, decreases from \alpha_{4.8}^{8.6}=0.11
between 4.8 and 8.6 GHz to \alpha_{8.6}^{20}=-0.16 between 8.6 and 20 GHz),
even if the sample is dominated by flat spectra sources (85 per cent of the
sample has \alpha_{8.6}^{20}>-0.5). The almost simultaneous spectra in total
intensity and polarisation allowed us a comparison of the polarised and total
intensity spectra: polarised fraction slightly increases with frequency, but
the shapes of the spectra have little correlation. Optical identifications
provided an estimation of redshift for 186 sources with a median value of 1.20
and 0.13 respectively for QSO and galaxies.Comment: 34 pages, 19 figures, tables of data included, replaced with version
published in MNRA
The Australia Telescope 20 GHz Survey: The Source Catalogue
We present the full source catalogue from the Australia Telescope 20 GHz
(AT20G) Survey. The AT20G is a blind radio survey carried out at 20 GHz with
the Australia Telescope Compact Array (ATCA) from 2004 to 2008, and covers the
whole sky south of declination 0 deg. The AT20G source catalogue presented here
is an order of magnitude larger than any previous catalogue of high-frequency
radio sources, and includes 5890 sources above a 20 GHz flux-density limit of
40 mJy. All AT20G sources have total intensity and polarisation measured at 20
GHz, and most sources south of declination -15 deg also have near-simultaneous
flux-density measurements at 5 and 8 GHz. A total of 1559 sources were detected
in polarised total intensity at one or more of the three frequencies. We detect
a small but significant population of non-thermal sources that are either
undetected or have only weak detections in low-frequency catalogues. We
introduce the term Ultra-Inverted Spectrum (UIS) to describe these radio
sources, which have a spectral index alpha(5, 20) > +0.7 and which constitute
roughly 1.2 per cent of the AT20G sample. The 20 GHz flux densities measured
for the strongest AT20G sources are in excellent agreement with the WMAP 5-year
source catalogue of Wright et al. (2009), and we find that the WMAP source
catalogue is close to complete for sources stronger than 1.5 Jy at 23 GHz.Comment: 21 pages, accepted for publication in MNRA
XGAP: a uniform and extensible data model and software platform for genotype and phenotype experiments.
We present an extensible software model for the genotype and phenotype community, XGAP. Readers can download a standard XGAP (http://www.xgap.org) or auto-generate a custom version using MOLGENIS with programming interfaces to R-software and web-services or user interfaces for biologists. XGAP has simple load formats for any type of genotype, epigenotype, transcript, protein, metabolite or other phenotype data. Current functionality includes tools ranging from eQTL analysis in mouse to genome-wide association studies in humans.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are
Galactic synchrotron emissivity measurements between 250° < l < 355° from the GLEAM survey with the MWA
Synchrotron emission pervades the Galactic plane at low radio frequencies, originating from cosmic ray electrons interacting with the Galactic magnetic field. Using a low-frequency radio telescope, the Murchison Widefield Array (MWA), we measure the free-free absorption of this Galactic synchrotron emission by intervening HII regions along the line of sight. These absorption measurements allow us to calculate the Galactic cosmic ray electron emissivity behind and in front of 47 detected HII regions in the region 250° < l < 355°, |b| < 2°.We find that all average emissivities between the HII regions and the Galactic edge along the line of sight (εb) are in the range of 0.39 ~ 1.45 K pc-1 with a mean of 0.77 K pc-1 and a variance of 0.14 K pc-1 at 88 MHz. Our best model, the two-circle model, divides the Galactic disc into three regions using two circles centring on the Galactic Centre. It shows a high emissivity region near the Galactic Centre, a low emissivity region near the Galactic edge, and a medium emissivity region between these two regions, contrary to the trend found by previous studiesSupport for the operation of the MWA is provided by
the Australian Government (NCRIS), under a contract to Curtin
University administered by Astronomy Australia Limited. We acknowledge the Pawsey Supercomputing Centre which is supported
by the Western Australian and Australian Governments. HS thanks
the support from the NSFC (11473038, 11273025). CAJ thanks the
Department of Science, Office of Premier & Cabinet, WA for their
support through the Western Australian Fellowship Program
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