Analysis of maize photosyntheis parameters and whole plant oxidative damage under long-term drought.

We test if maize maintain yield under long-term drought throught improvement of photosyntheis (A) coupled with up-regulation of the antioxidant system induced by increase in levels of abscisic acid (ABA). Four maize genotypes with constrasting drought tolerance: BRS1010 and 2B710 (sensitive) and DKB 390 and BRS1055 (tolerant) in two soil water levels, field capacity (FC) and water deficit (WD) were used. WD was applied at the pre-flowering stage for 12 days, and oxidative damage was measured as malondialdehyde (MDA) accumulation in whole plant. Plants from tolerant genotypes DKB390 and BRS1055 showed higher A and had no signal of oxidative damage compared to sensitive genotypes 2B710 and BRS1010 under WD, resulting in a higher yield attributes. For our surprising, it was dissociated from up-regulation of the antioxidant system ABA-mediated. In turn, plants from two sensitive genotypes under WD showed compared to FC consistent reduction of A due to mesophyll conductance (gm) limitation. Only WD plants from sensitive genotype BRS1010 presented leaf ABA levels increased related to its counterparts under FC; however, due to the inactivation of catalase activity the oxidative damage control was not effective, resulting a hardly MDA acumulation in both leaves and roots. The maize tolerance under long-term drought is linked to scape of gm decline

Galaxy properties from J-PAS narrow-band photometry

We study the consistency of the physical properties of galaxies retrieved from SED-fitting as a function of spectral resolution and signal-to-noise ratio (SNR). Using a selection of physically motivated star formation histories, we set up a control sample of mock galaxy spectra representing observations of the local universe in high-resolution spectroscopy, and in 56 narrow-band and 5 broad-band photometry. We fit the SEDs at these spectral resolutions and compute their corresponding the stellar mass, the mass- and luminosity-weighted age and metallicity, and the dust extinction. We study the biases, correlations, and degeneracies affecting the retrieved parameters and explore the r\^ole of the spectral resolution and the SNR in regulating these degeneracies. We find that narrow-band photometry and spectroscopy yield similar trends in the physical properties derived, the former being considerably more precise. Using a galaxy sample from the SDSS, we compare more realistically the results obtained from high-resolution and narrow-band SEDs (synthesized from the same SDSS spectra) following the same spectral fitting procedures. We use results from the literature as a benchmark to our spectroscopic estimates and show that the prior PDFs, commonly adopted in parametric methods, may introduce biases not accounted for in a Bayesian framework. We conclude that narrow-band photometry yields the same trend in the age-metallicity relation in the literature, provided it is affected by the same biases as spectroscopy; albeit the precision achieved with the latter is generally twice as large as with the narrow-band, at SNR values typical of the different kinds of data.Comment: 26 pages, 15 figures. Accepted for publication in MNRA

Desempenho da cultura do feijão-caupi sob efeitos de adubos verdes em Itaquiraí, Mato Grosso do Sul.

bitstream/item/68912/1/043-moitinho-desempenho.pdfPublicado também no Cadernos de Agroecologia, v. 7, n.2, 2012

Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study.Brazilian Research Funding InstitutionsCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Pathogen- and Host-Directed Antileishmanial Effects Mediated by Polyhexanide (PHMB)

BACKGROUND:Cutaneous leishmaniasis (CL) is a neglected tropical disease caused by protozoan parasites of the genus Leishmania. CL causes enormous suffering in many countries worldwide. There is no licensed vaccine against CL, and the chemotherapy options show limited efficacy and high toxicity. Localization of the parasites inside host cells is a barrier to most standard chemo- and immune-based interventions. Hence, novel drugs, which are safe, effective and readily accessible to third-world countries and/or drug delivery technologies for effective CL treatments are desperately needed. METHODOLOGY/PRINCIPAL FINDINGS:Here we evaluated the antileishmanial properties and delivery potential of polyhexamethylene biguanide (PHMB; polyhexanide), a widely used antimicrobial and wound antiseptic, in the Leishmania model. PHMB showed an inherent antileishmanial activity at submicromolar concentrations. Our data revealed that PHMB kills Leishmania major (L. major) via a dual mechanism involving disruption of membrane integrity and selective chromosome condensation and damage. PHMB's DNA binding and host cell entry properties were further exploited to improve the delivery and immunomodulatory activities of unmethylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODN). PHMB spontaneously bound CpG ODN, forming stable nanopolyplexes that enhanced uptake of CpG ODN, potentiated antimicrobial killing and reduced host cell toxicity of PHMB. CONCLUSIONS:Given its low cost and long history of safe topical use, PHMB holds promise as a drug for CL therapy and delivery vehicle for nucleic acid immunomodulators

Modeling rare gene variation to gain insight into the oldest biomarker in autism: construction of the serotonin transporter Gly56Ala knock-in mouse

Alterations in peripheral and central indices of serotonin (5-hydroxytryptamine, 5-HT) production, storage and signaling have long been associated with autism. The 5-HT transporter gene (HTT, SERT, SLC6A4) has received considerable attention as a potential risk locus for autism-spectrum disorders, as well as disorders with overlapping symptoms, including obsessive-compulsive disorder (OCD). Here, we review our efforts to characterize rare, nonsynonymous polymorphisms in SERT derived from multiplex pedigrees carrying diagnoses of autism and OCD and present the initial stages of our effort to model one of these variants, Gly56Ala, in vivo. We generated a targeting vector to produce the Gly56Ala substitution in the Slc6a4 locus by homologous recombination. Following removal of a neomycin resistance selection cassette, animals exhibiting germline transmission of the Ala56 variant were bred to establish a breeding colony on a 129S6 background, suitable for initial evaluation of biochemical, physiological and behavioral alterations relative to SERT Gly56 (wildtype) animals. SERT Ala56 mice were achieved and exhibit a normal pattern of transmission. The initial growth and gross morphology of these animals is comparable to wildtype littermate controls. The SERT Ala56 variant can be propagated in 129S6 mice without apparent disruption of fertility and growth. We discuss both the opportunities and challenges that await the physiological/behavioral analysis of Gly56Ala transgenic mice, with particular reference to modeling autism-associated traits

J-PAS: forecasts on interacting vacuum energy models

The next generation of galaxy surveys will allow us to test some fundamental aspects of the standard cosmological model, including the assumption of a minimal coupling between the components of the dark sector. In this paper, we present the Javalambre Physics of the Accelerated Universe Astrophysical Survey (J-PAS) forecasts on a class of unified models where cold dark matter interacts with a vacuum energy, considering future observations of baryon acoustic oscillations, redshift-space distortions, and the matter power spectrum. After providing a general framework to study the background and linear perturbations, we focus on a concrete interacting model without momentum exchange by taking into account the contribution of baryons. We compare the J-PAS results with those expected for DESI and Euclid surveys and show that J-PAS is competitive to them, especially at low redshifts. Indeed, the predicted errors for the interaction parameter, which measures the departure from a $\Lambda$CDM model, can be comparable to the actual errors derived from the current data of cosmic microwave background temperature anisotropies.Comment: 34 pages, 9 figures, 14 table