158 research outputs found
Optimization of Experimental Model Parameter Identification for Energy Storage Systems
The smart grid approach is envisioned to take advantage of all available modern technologies in transforming the current power system to provide benefits to all stakeholders in the fields of efficient energy utilisation and of wide integration of renewable sources. Energy storage systems could help to solve some issues that stem from renewable energy usage in terms of stabilizing the intermittent energy production, power quality and power peak mitigation. With the integration of energy storage systems into the smart grids, their accurate modeling becomes a necessity, in order to gain robust real-time control on the network, in terms of stability and energy supply forecasting. In this framework, this paper proposes a procedure to identify the values of the battery model parameters in order to best fit experimental data and integrate it, along with models of energy sources and electrical loads, in a complete framework which represents a real time smart grid management system. The proposed method is based on a hybrid optimisation technique, which makes combined use of a stochastic and a deterministic algorithm, with low computational burden and can therefore be repeated over time in order to account for parameter variations due to the battery's age and usage
FPGA-based real time compensation method for medium voltage transducers
open5noSince the increase of the distributed power connected to the medium voltage networks, a capillary monitoring of the power quality
becomes essential. This entails the spread of transducers with suitable frequency bandwidths, as required by the relevant standards.
The paper describes a real time compensation method for the extension of the frequency bandwidth of medium voltage dividers
whose performances do not allow to perform measurements over a wide frequency range. This approach will contribute to keep the
costs of this innovation lowopenCrotti, Gabriella; Gallo, Daniele; Giordano, Domenico; Landi, Carmine; Inrim, Mario Luiso2 1Crotti, Gabriella; Gallo, Daniele; Giordano, Domenico; Landi, Carmine; Inrim, Mario Luiso2
Effect of auxotrophies on yeast performance in aerated fed-batch reactor
A systematic investigation on the effects of auxotrophies on the performance of yeast in aerated fed-batch reactor was carried out. Six isogenic strains from the CEN.PK family of Saccharomyces cerevisiae,one prototroph and five auxotrophs, were grown in aerated fed-batch reactor using the same operative conditions and a proper nutritional supplementation. The performance of the strains, in terms of final biomass decreased with increasing the number of auxotrophies. Auxotrophy for leucine exerted a profound negative effect on the performance of the strains. Accumulation of reactive oxygen species (ROS) in the cells of the strain carrying four auxotrophies and its significant viability loss, were indicative of an oxidative stress response induced by exposure of cells to the environmental conditions. The mathematical model was fundamental to highlight how the carbon flux, depending on the number and type of auxotrophies, was diverted towards the production of increasingly large quantities of energy for maintenance
Evaluation of Voltage Transformers’ Accuracy in Harmonic and Interharmonic Measurement
The measurement of Power Quality (PQ), generally performed at Low Voltage (LV) level, is gaining more and more importance also at the Medium Voltage (MV) level, due to the increasing presence of switching power converters (both loads or generators) directly connected to MV grids. In this case, the use of Voltage Transformers (VTs) is unavoidable to scale voltage down to amplitudes compatible with the input ranges of PQ instruments. However, the current absence of an international standard dealing with VTs used for PQ measurements leaves the manufacturers and the users in a situation of complete uncertainty, since different products can have performance specifications tested and stated in completely different ways. This paper aims at defining an integrated approach for the evaluation of VTs accuracy used for PQ measurements, focusing on harmonics and interharmonics. The paper provides experimental results of the tests performed on a MV inductive VT according to the proposed procedure. As a result, it is demonstrated that VT accuracy in the measurement of a specific phenomenon should be evaluated with complex waveforms including the contemporary presence of different PQ phenomena
Effect of auxotrophies on yeast growth in aerated fed-batch reactor
Mutant and deletion strains of the yeast Saccharomyces cerevisiae
having one/several auxotrophies are largely used in the
development of recombinant strains for heterologous protein
production because they ensure maintenance of plasmids with
selectable markers. The production is usually carried out by culturing the recombinant strain in aerated fed-batch, where sugar
limitation achieves high yields of biomass and product.
In a previous work, it was evidenced that growth of the auxotrophic S. cerevisiae BY4741 (MATa, ura30, leu20, met150,
his31) engineered for human IL-1 production, and employed
in aerated fed-batch, early arrested even in the presence of a
correct nutritional complementation (being specific nutrients for
genetically uncomplemented auxotrophies provided in no growthlimiting amounts). It was assumed that this behaviour may depend on the high number of auxotrophies, since the prototrophic S288C, from which BY4741 derives, showed a typical performance under the same cultivation mode. Therefore, a systematic investigation on the effect of auxotrophies on yeast growth in aerated fed-batch was carried out. Four isogenic strains of the CEN.PK family, with a progressively increasing number of auxotrophies (from one to four) were assayed under fed-batch conditions and a proper nutritional complementation. Feeding to the reactor was exponentially increased imposing a specific growth rate below the critical one. The behaviour of the auxotrophic strains was compared with that of the isogenic prototrophic strain. by evaluating the capacity to keep the specific growth rate chosen. A clear correlation among optimum growth and number of auxotrophies has been found. Furthermore we have investigated the possible effect of the type of auxotrophy (ura- or leu-) on the strain performance, monitoring as well cell viability of each strain. The study is a contribution to know the phenotypic effects of auxotrophies in yeast and can have implications for biotechnological applications
A Web Service Interface for a Distributed Measurement System Based on Decentralized Sharing Network 1
Abstract: The Web Service technology has increased in importance in these years. Accessing to remote resources without knowledge about physical implementation and with a reduced hardware requirement is the main goal of new portable device. The use of Web Service technology allows clients to create a standard interface to access to the measurement service published by the server. In low cost multipoint distributed measurement systems, the measurement services are provided by Smart Web Sensors. A new concept of distributed measurement system arises from the possibility to fuse all services with the same functionality in a single user-transparent service: the response of distributed services network to a user request gives a complete vision of the service by collecting results from any Smart Web Sensor in the network. So, in this paper the development of a multipoint distributed measurement system, based on the peer-to-peer Gnutella network with Web Service interface, is presented. Copyright © 2013 IFSA
On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
Background: The selection of new yeast strains could lead to
improvements in bioethanol production. Here, we have studied the
fermentative capacity of different auxotrophic mutants of Saccharomyces
cerevisiae , which are routinely used as hosts for the production of
heterologous proteins. It has recently been found that these strains
exhibit physiological alterations and peculiar sensitivities with
respect to the parental prototrophic strains from which they derive. In
this work the performance of auxotrophic S. cerevisiae CEN.PK strains
was compared to the corresponding prototrophic strain, to S. cerevisiae
T5bV, a strain isolated from grape must and to another auxotrophic
strain, S. cerevisiae BY4741. Results: The results indicate that the
fermentative capacity of strains grown in 2% glucose was similar in all
the strains tested. However, in 15% initial glucose, the auxotrophic
strains exhibited a more than doubled ethanol yield on biomass (10 g
g-1dw) compared to the prototrophic strains (less than 5 g g-1dw).
Other tests have also evidenced that in medium depletion conditions,
ethanol production continues after growth arrest. Conclusions: The
results highlight the capacity of auxotrophic yeast strains to produce
ethanol per mass unit, in a higher amount with respect to the
prototrophic ones. This leads to potential applications for auxotrophic
strains of S. cerevisiae in the production of ethanol in both
homogeneous and heterogeneous phases (immobilized systems). The higher
ethanol yield on biomass would be advantageous in immobilized cell
systems, as a reduced yeast biomass could greatly reduce the mass
transfer limitations through the immobilization matrix
Fast Hybrid MPPT Technique for Photovoltaic Applications: Numerical and Experimental Validation
In PV applications, under mismatching conditions, it is necessary to adopt a maximum power point tracking (MPPT) technique which is able to regulate not only the voltages of the PV modules of the array but also the DC input voltage of the inverter. Such a technique can be considered a hybrid MPPT (HMPPT) technique since it is neither only distributed on the PV modules of the PV array or only centralized at the input of the inverter. In this paper a new HMPPT technique is presented and discussed. Its main advantages are the high MPPT efficiency and the high speed of tracking which are obtained by means of a fast estimate of the optimal values of PV modules voltages and of the input inverter voltage. The new HMPPT technique is compared with simple HMPPT techniques based on the scan of the power versus voltage inverter input characteristic. The theoretical analysis and the results of numerical simulations are widely discussed. Moreover, a laboratory test system, equipped with PV emulators, has been realized and used in order to experimentally validate the proposed technique
- …