A Narrative Inquiry into the Learning Experiences of Deaf Individuals in Tennis

Deaf individuals do not have equal access to participating in sports as their hearing counterparts in the United States. Tennis is one sport to which this lack of access applies. The purpose of this qualitative, narrative research study was to add to the research literature by illuminating the learning experiences of Deaf individuals in tennis in the United States. This study was built on a tripartite conceptual framework consisting of motivation, interaction, and the flow experience. The study explored how tennis influenced other aspects in the lives of Deaf individuals. These other aspects included exposure to the game, learning opportunities, underlying motivations, and social interaction. Participants were 9 Deaf adults who had tennis playing experience. Data were collected from the Deaf Tennis Profile Questionnaire, Deaf Tennis Experience Questionnaire, and a Deaf Tennis Videophone Interview. A priori typological and inductive analyses were used to search for themes and patterns from the data. Findings indicated that learning the game of tennis was relevant to participants\u27 lives. As individuals experienced improvements in their tennis abilities, positive changes in self-perceptions occurred. Participants reported that experiencing the highs and lows while playing tennis increased their motivation and social interaction. Insights from these Deaf tennis participants will give administrators, physical education teachers, coaches, and recreation professionals a better understanding of more effective ways to provide tennis instruction and skills. Self-confident, skillful Deaf tennis players are better contributors to society as a whole. Participation in sports enhances the confidence, self-worth, self-image, and interaction skills of Deaf persons

Mn(II)-oxidizing Bacteria are Abundant and Environmentally Relevant Members of Ferromanganese Deposits in Caves of the Upper Tennessee River Basin

The upper Tennessee River Basin contains the highest density of our nation's caves; yet, little is known regarding speleogenesis or Fe and Mn biomineralization in these predominantly epigenic systems. Mn:Fe ratios of Mn and Fe oxide-rich biofilms, coatings, and mineral crusts that were abundant in several different caves ranged from ca. 0.1 to 1.0 as measured using ICP-OES. At sites where the Mn:Fe ratio approached 1.0 this represented an order of magnitude increase above the bulk bedrock ratio, suggesting that biomineralization processes play an important role in the formation of these cave ferromanganese deposits. Estimates of total bacterial SSU rRNA genes in ferromanganese biofilms, coatings, and crusts measured approximately 7×107–9×109 cells/g wet weight sample. A SSU-rRNA based molecular survey of biofilm material revealed that 21% of the 34 recovered dominant (non-singleton) OTUs were closely related to known metal-oxidizing bacteria or clones isolated from oxidized metal deposits. Several different isolates that promote the oxidation of Mn(II) compounds were obtained in this study, some from high dilutions (10–8–10–10) of deposit material. In contrast to studies of caves in other regions, SSU rRNA sequences of Mn-oxidizing bacterial isolates in this study most closely matched those of Pseudomonas, Leptothrix, Flavobacterium, and Janthinobacterium. Combined data from geochemical analyses, molecular surveys, and culture-based experiments suggest that a unique consortia of Mn(II)-oxidizing bacteria are abundant and promoting biomineralization processes within the caves of the upper Tennessee River Basin

Sustained Anthropogenic Impact in Carter Saltpeter Cave, Carter County, Tennessee and the Potential Effects On Manganese Cycling

Anthropogenic impact is a pervasive problem in heavily trafficked cave systems and fecal contamination is equally problematic in many cave and karst waters worldwide. Carter Saltpeter Cave in Carter County, Tennessee exhibits Mn(III/IV) oxide coatings associated with groundwater seeps, as well as manganese oxide growth on litter. Culturing results revealed that Mn(III/IV) oxide production on litter was associated with Mn(II)-oxidizing fungi. Immediately prior to this study, a massive Mn(II)-oxidizing biofilm bloomed at a cave seep. During the course of this study from 2009–2011, the seep exhibited a dramatic visual reduction in Mn(III/IV) oxide production, which was hypothesized to correlate with a decrease in fecal nutrient input. Molecular methods (16S rRNA gene sequencing) confirmed the presence of Bacteroides-Prevotella human fecal indicators in this seep, and most probable number assays and ion chromatography of the associated seep water confirmed nutrient loading at the site. Further, phylogenetic analysis from clone sequences suggested a strong initial human-specific fecal signature (50% of the sequences clustering with human feces sequences) in July 2009, and a weaker human signature (20% clustering) by June 2011. Most Probable Number (MPN) analyses of heterotrophic bacteria at this site suggested that Mn(II) oxidation was correlated with heterotrophic activity, due to point source exogenous nutrient loading

Explaining student achievement: the influence of teachers' pedagogical content knowledge in statistics

Statistics is an increasingly important component of the mathematics curriculum. StatSmart was a project intended to influence middle-years students’ learning outcomes in statistics through the provision of appropriate professional learning opportunities and technology to teachers. Participating students in grade 5/6 to grade 9 undertook three tests, a pre-test, a post-test and a longitudinal retention test over a period of 2 years. Their teachers completed a survey that included items measuring pedagogical content knowledge (PCK) for teaching statistics. Despite the development of valid instruments to measure both student and teacher content knowledge and teachers’ PCK, linking teachers’ knowledge directly to students’ learning outcomes has proved elusive. Multilevel modelling of results from 789 students for whom there were 3 completed tests and measures from their teachers indicated that students’ outcomes were influenced positively by their initial teacher’s PCK. Extended participation of teachers in the project also appeared to reduce negative effects of changing teachers

Mn(II)-Oxidizing Bacteria Are Abundant And Environmentally Relevant Members Of Ferromanganese Deposits In Caves Of The Upper Tennessee River Basin

The upper Tennessee River Basin contains the highest density of our nation’s caves; yet, little is known regarding speleogenesis or Fe and Mn biomineralization in these predominantly epigenic systems. Mn:Fe ratios of Mn and Fe oxide-rich bio?lms, coatings, and mineral crusts that were abundant in several different caves ranged from ca. 0.1 to 1.0 as measured using ICP-OES. At sites where the Mn:Fe ratio approached 1.0 this represented an order of magnitude increase above the bulk bedrock ratio, suggesting that biomineralization processes play an important role in the formation of these cave ferromanganese deposits. Estimates of total bacterial SSU rRNA genes in ferromanganese bio?lms, coatings, and crusts measured approximately 7×107–9×109 cells/g wet weight sample. A SSU-rRNA based molecular survey of bio?lm material revealed that 21% of the 34 recovered dominant (non-singleton) OTUs were closely related to known metal-oxidizing bacteria or clones isolated from oxidized metal deposits. Several different isolates that promote the oxidation of Mn(II) compounds were obtained in this study, some from high dilutions (10–8–10–10) of deposit material. In contrast to studies of caves in other regions, SSU rRNA sequences of Mn-oxidizing bacterial isolates in this study most closely matched those of Pseudomonas, Leptothrix, Flavobacterium, and Janthinobacterium. Combined data from geochemical analyses, molecular surveys, and culture-based experiments suggest that a unique consortia of Mn(II)-oxidizing bacteria are abundant and promoting biomineralization processes within the caves of the upper Tennessee River Basin

Geomicrobiology of Ferromanganese Deposits in Caves of the upper Tennessee River Basin

Ferromanganese deposits in epigenic cave systems located within the Knox Dolomite bedrock formation of the upper Tennessee River Basin were analyzed to determine the role of microbes in the cycling of manganese and the transformation of cave mineralogy. Results from geochemical analyses, molecular surveys, and culture-dependent surveys indicate that Mn(II)-oxidizing bacteria are abundant and environmentally relevant members of ferromanganese deposits in the study area, and that these cave systems harbor unique microbial communities. Over the duration of the study period (July 2009-2011), a decline in the intensity of ferromanganese deposits in a cave biofilm was noted. Because a sewage discharge may have occurred at this site prior to July 2009, it was hypothesized that the decline may have been associated with the gradual abatement of an acute, point source of pollution. The change in biofilm appearance combined with water chemistry data and molecular-based and culture-dependent results suggest an association between the bloom of Mn(II)-oxidizing bacteria and a nutrient loading event. More work is needed to determine if blooms of Mn(II)-oxidizing bacteria are widespread where contamination is prevalent. However, if this hypothesis is validated, monitoring of Mn-oxidizing microbial communities would provide a method of assessing anthropogenic impact in shallow cave systems

Microbial Sequencing Analyses Suggest the Presence of a Fecal Veneer on Indoor Climbing Wall Holds

Artifcial climbing walls represent a unique indoor environment in which humans interact closely with a variety of surface types. Climbing wall holds may mediate transmission of organisms between individuals, and yet there are no studies that identify microorganisms present on these surfaces. In the current study, the micro-organisms found on climbing wall holds were characterized by analysis of ampli?ed SSU rRNA gene sequences. In contrast to many other studies of built environments, the majority of microorganisms on holds were most closely related to microbes annotated as being recovered from environmental sources, such as soil, with human skin also representing an important source. Regional patterns were evident as rRNA gene sequences from the marine cyano-bacterium Prochlorococcus were abundant in gyms found within 16 km of the ocean. Enterobacteriaceae were present on 100 % of holds surveyed, and the members detected are commonly associated with fecal matter

Standing dead trees are a conduit for the atmospheric flux of CH4 and CO2 from wetlands

In vegetated wetland ecosystems, plants can be a dominant pathway in the atmospheric flux of methane, a potent greenhouse gas. Although the roles of herbaceous vegetation and live woody vegetation in this flux have been established, the role of dead woody vegetation is not yet known. In a restored wetland of North Carolina’s coastal plain, static flux chambers were deployed at two heights on standing dead trees to determine if these structures acted as a conduit for methane emissions. Methane fluxes to the atmosphere were measured in five of the chambers, with a mean flux of 0.4±0.1 mg m-2 h-1. Methane consumption was also measured in three of the chambers, with a mean flux of -0.6±0.3 mg m-2 h-1. Standing dead trees were also a source of the flux of CO2 (114.6±23.8 mg m-2 h-1) to the atmosphere. Results confirm that standing dead trees represent a conduit for the atmospheric flux of carbon gases from wetlands. However, several questions remain regarding the ultimate source of these carbon gases, the controls on the magnitude and direction of this flux, the mechanisms that induce this flux, and the importance of this pathway relative to other sources at the landscape level

Increased Abundance Of Gallionella Spp., Leptothrix Spp. And Total Bacteria In Response To Enhanced Mn And Fe Concentrations In A Disturbed Southern Appalachian High Elevation Wetland

The Sorrento wetland hosts several Fe- and Mn-rich seeps that are reported to have appeared after the area was disturbed by recent attempts at development. Culture-independent and culture-based analyses were utilized to characterize the microbial community at the main site of the Fe and Mn seep. Several bacteria capable of oxidizing Mn(II) were isolated, including members related to the genera Bacillus, Lysinibacillus, Pseudomonas, and Leptothrix,but none of these were detected in clone libraries. Most probable number assays demonstrated that seep and wetland sites contained higher numbers of culturable Mn-oxidizing microorganisms than an upstream reference site. When compared with quantitative real time PCR (qPCR) assays of total bacteria, MPN analyses indicated that less than 0.01% of the total population (estimated around 109 cells/g) was culturable. Light microscopy and fluorescence in situ hybridization (FISH) images revealed an abundance of morphotypes similar to Fe- and Mn-oxidizing Leptothrixspp. and Gallionella spp. in seep and wetland sites. FISH allowed identification of Leptothrix-type sheath-forming organisms in seep samples but not in reference samples. Gallionella spp. and Leptothrixspp. cells numbers were estimated using qPCR with a novel primer set that we designed. Results indicated that numbers of Gallionella,Leptothrix or total bacteria were all significantly higher at the seep site relative to the reference site (where Gallionella was below detection). Interestingly, numbers of Leptothrix in the seep site were estimated at only 107 cells/g and were not statistically different in the late summer versus the late winter, despite dramatic changes in sheath abundance (as indicated by microscopy). qPCRalso indicated that Gallionella spp. may represent up to 10% (3 ×108 cells/g) of the total bacteria in seep samples. These data corroborate clone library data from samples taken in October 2008, where 11 SSU rRNA sequences related to Gallionella spp. were detected out of 77 total sequences (roughly 10–15%), and where Leptothrix sequences were not detected. Analysis of this SSU rRNA clonal library revealed that a diverse microbial community was present at seep sites. At a 3%difference cutoff, 30 different operational taxonomic units were detected out of 77 sequences analyzed. Dominant sequence types clustered among the beta- and gamma- Proteobacteria near sequences related to the genera Ideonella, Rhodoferax,Methylotenera, Methylobacter, andGallionella.Overall, results suggest that high metal concentrations at the seep sites have enriched for Fe- and Mn-oxidizing bacteria including organisms related to Gallionella and Leptothrix species, and that members of these genera coexist within a diverse microbial community