701 research outputs found
Book Reviews
Reviews of the following books: Reflections in Bullongh’s Pond: Economy and Ecosystem in New England by Diana Muir; Maine in America: American Art at the Farnsworth Art Museum. By Pamela J. Belanger; foreword by Christopher B. Crosman; essay by William H. Gerdt
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Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates.
Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples without detectable levels of mercury. Phylogenetic analyses of the plasmid-encoded mercury reductases from the studied groundwater site show them to be distinct from those reported in proximal metal-contaminated sites. We synthesized the entire native plasmid and demonstrated that the plasmid was sufficient to confer functional mercury resistance in Escherichia coli Given the possibility that natural transformation is a prevalent HGT mechanism in the low-cell-density environments of groundwaters, we also assayed bacterial strains from this environment for competence. We used the native plasmid-encoded metal resistance to design a screen and identified 17 strains positive for natural transformation. We selected 2 of the positive strains along with a model bacterium to fully confirm HGT via natural transformation. From an ecological perspective, the role of the native plasmid population in providing advantageous traits combined with the microbiome's capacity to take up environmental DNA enables rapid adaptation to environmental stresses.IMPORTANCE Horizontal transfer of mobile genetic elements via natural transformation has been poorly understood in environmental microbes. Here, we confirm the functionality of a native plasmid-encoded mercury resistance operon in a model microbe and then query for the dissemination of this resistance trait via natural transformation into environmental bacterial isolates. We identified 17 strains including Gram-positive and Gram-negative bacteria to be naturally competent. These strains were able to successfully take up the plasmid DNA and obtain a clear growth advantage in the presence of mercury. Our study provides important insights into gene dissemination via natural transformation enabling rapid adaptation to dynamic stresses in groundwater environments
Self-Similarity of Friction Laws
The change of the friction law from a mesoscopic level to a macroscopic level
is studied in the spring-block models introduced by Burridge-Knopoff. We find
that the Coulomb law is always scale invariant. Other proposed scaling laws are
only invariant under certain conditions.}Comment: Plain TEX. Figures not include
Book Reviews
Reviews of the following books: A Brides Passage: Susan Hathorns Year Under Sail edited by Catherine Petroski; Inventing Acadia: Artists and Tourists at Mount Desert by Pamela J. Belanger; Migration and the Origins of the English Atlantic World by Alison Games; Johnson\u27s Kingdom: The Story of a Nineteenth Century Industrial Kingdom in the Town of Wayne, Maine by Edward Kallop; Saltwater Foodways: New Englanders and Their Food, at Sea and Ashore in the Nineteenth Century By Sandra L. Olive
Inhibition of microbial sulfate reduction in a flow-through column system by (per)chlorate treatment.
Microbial sulfate reduction is a primary cause of oil reservoir souring. Here we show that amendment with chlorate or perchlorate [collectively (per)chlorate] potentially resolves this issue. Triplicate packed columns inoculated with marine sediment were flushed with coastal water amended with yeast extract and one of nitrate, chlorate, or perchlorate. Results showed that although sulfide production was dramatically reduced by all treatments, effluent sulfide was observed in the nitrate (10 mM) treatment after an initial inhibition period. In contrast, no effluent sulfide was observed with (per)chlorate (10 mM). Microbial community analyses indicated temporal community shifts and phylogenetic clustering by treatment. Nitrate addition stimulated Xanthomonadaceae and Rhizobiaceae growth, supporting their role in nitrate metabolism. (Per)chlorate showed distinct effects on microbial community structure compared with nitrate and resulted in a general suppression of the community relative to the untreated control combined with a significant decrease in sulfate reducing species abundance indicating specific toxicity. Furthermore, chlorate stimulated Pseudomonadaceae and Pseudoalteromonadaceae, members of which are known chlorate respirers, suggesting that chlorate may also control sulfidogenesis by biocompetitive exclusion of sulfate-reduction. Perchlorate addition stimulated Desulfobulbaceae and Desulfomonadaceae, which contain sulfide oxidizing and elemental sulfur-reducing species respectively, suggesting that effluent sulfide concentrations may be controlled through sulfur redox cycling in addition to toxicity and biocompetitive exclusion. Sulfur isotope analyses further support sulfur cycling in the columns, even when sulfide is not detected. This study indicates that (per)chlorate show great promise as inhibitors of sulfidogenesis in natural communities and provides insight into which organisms and respiratory processes are involved
Fragmentation of exotic oxygen isotopes
Abrasion-ablation models and the empirical EPAX parametrization of projectile fragmentation are described. Their cross section predictions are compared to recent data of the fragmentation of secondary beams of neutron-rich, unstable 19,20,21O isotopes at beam energies near 600 MeV/nucleon as well as data for stable 17,18O beams
Effective action approach and Carlson-Goldman mode in d-wave superconductors
We theoretically investigate the Carlson-Goldman (CG) mode in two-dimensional
clean d-wave superconductors using the effective ``phase only'' action
formalism. In conventional s-wave superconductors, it is known that the CG mode
is observed as a peak in the structure factor of the pair susceptibility
only just below the transition temperature T_c and only
in dirty systems. On the other hand, our analytical results support the
statement by Y.Ohashi and S.Takada, Phys.Rev.B {\bf 62}, 5971 (2000) that in
d-wave superconductors the CG mode can exist in clean systems down to the much
lower temperatures, . We also consider the manifestations of
the CG mode in the density-density and current-current correlators and discuss
the gauge independence of the obtained results.Comment: 23 pages, RevTeX4, 12 EPS figures; final version to appear in PR
Identification of Radiopure Titanium for the LZ Dark Matter Experiment and Future Rare Event Searches
The LUX-ZEPLIN (LZ) experiment will search for dark matter particle
interactions with a detector containing a total of 10 tonnes of liquid xenon
within a double-vessel cryostat. The large mass and proximity of the cryostat
to the active detector volume demand the use of material with extremely low
intrinsic radioactivity. We report on the radioassay campaign conducted to
identify suitable metals, the determination of factors limiting radiopure
production, and the selection of titanium for construction of the LZ cryostat
and other detector components. This titanium has been measured with activities
of U~1.6~mBq/kg, U~0.09~mBq/kg,
Th~~mBq/kg, Th~~mBq/kg, K~0.54~mBq/kg, and Co~0.02~mBq/kg (68\% CL).
Such low intrinsic activities, which are some of the lowest ever reported for
titanium, enable its use for future dark matter and other rare event searches.
Monte Carlo simulations have been performed to assess the expected background
contribution from the LZ cryostat with this radioactivity. In 1,000 days of
WIMP search exposure of a 5.6-tonne fiducial mass, the cryostat will contribute
only a mean background of (stat)(sys) counts.Comment: 13 pages, 3 figures, accepted for publication in Astroparticle
Physic
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